QIAxcel Advanced System

DNAフラグメントおよびRNAの容易な解析

S_2120_IAS_QIAxcel_Advanced_s

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QIAxcel Advanced Instrument

Cat. No. / ID:  9001941

Capillary electrophoresis device: delivered with computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor   
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QIAxcel Advanced
QIAxcel ScreenGel Software
The QIAxcel Advanced Instrument has been discontinued. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced Instrument, including providing associated repair services until December 31, 2029.
QIAxcel Advanced instrumentは、専用のQIAGEN キットと組み合わせて使用し、それぞれのキットハンドブックに記載されているアプリケーションで使用してください。 日本での型番とパッケージ内容は、QIAGEN機器総合カタログ をご参照ください。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • マニュアル操作なしに最大96サンプルの高速解析
  • 即使用可能なゲルカートリッジで安全かつ簡便なシステム
  • わずか0.1 ng/μl の核酸濃度でも正確な結果
  • 3~5 bpの高い分離能で標準化された正確な解析
  • 21 CFR Part 11 complianceに準拠したユーザーフレンドリーな解析ソフトウェア

Product Details

QIAxcel Advanced Systemは労力のかかる従来のDNA/RNAゲル解析に代わる革新的なシステムで、能率化したワークフローにより、結果を得るまでの時間を短縮します。QIAxcel Advanced Systemでは、1ランあたり最大96サンプルのキャピラリー電気泳動を高い分離能で完全自動化できます。12サンプルのDNAフラグメント解析をわずか3分で行なえます。即使用可能なゲルカートリッジを用いると、最小限のマニュアル作業で96サンプルを解析でき、マニュアル作業による間違いが減少し時間のかかるゲル調製も不要です。さらに、ユーザーフレンドリーなQIAxcel ScreenGel Softwareによりデータの解析とドキュメンテーションを簡便に行なえます。

Performance

能率的なワークフロー

QIAxcel Advanced Systemは、マニュアルによる電気泳動解析を排除することにより、DNAおよびRNA解析をスピードアップするだけでなく、同時にサンプル精製から解析までの全ワークフローを能率化します(図“能率的なワークフロー”)。QIAxcel Advanced を実証済みのエンドポイントPCR 用QIAGEN キットと組み合わせることによって、PCR フラグメントの解析を高い信頼性で実現するテスト済みの統合システムを提供します。 高い再現性ならびに時間とコストの大幅な節約が可能です。

1台で多彩なアプリケーションに対応

QIAxcel Advanced System は多様性のある電気泳動装置で、様々なアプリケーションを提供します。プログラミング済みのメソッドと対応するゲルカートリッジを組み合わせることにより、複数のPCRフラグメント、制限酵素反応により切断されたDNA、トータルRNA、cRNAなどの様々な核酸の分離と解析を実現します。ジェノタイピングアプリケーションにおいては、QIAxcel Advanced Systemは従来のアガロースゲル電気泳動に比べて、より正確なサイズ測定と高感度な検出を実現します(図“バクテリアの正確なハイスループットジェノタイピング”)。QIAxcel Advanced System では多数のサンプルを処理できるので、品質管理のために迅速な結果を必要とし、96ウェルによるRNA精製を行っているラボに最適です(図“精製したRNAの解析”)。市販のその他の装置は最大12サンプルまでしか同時に処理できませんが、QIAxcel Advanced Systemは一回の操作で96サンプルの処理を手作業なしに行なうことができます。

Principle

QIAxcel Advancedには発光ダイオードアレイやmicro-optical collectorが搭載されています。キャピラリー内のゲルマトリックスを泳動するフラグメントは励起および検出スポットを通過し、シグナルが光電子増倍管からQIAxcel ScreenGel Softwareにデータ解析のために転送されます(図“サンプルの分離プロセス”)。

QIAxcel Advancedの高感度検出により、低濃度の核酸でも正確な結果が得られます。0.5 kb 未満のフラグメントの分離能が3~5 bp であるQIAxcel Advanced System は、より確実なデータ判断ができるだけではなく、スラブゲル法よりもさら高い精度を実現します。解析に必要なサンプル量は0.1 μl 未満で、残りの貴重なサンプルはダウンストリームアプリケーションに使用可能です。サンプルの自動アプライや充填済みゲルカートリッジにより、エチジウムブロマイドなどの有害物質への接触が最小限に抑えられます(図“すぐに泳動が可能なカートリッジ”)。

分離能やスピードなどの様々なアプリケーションのニーズに対応したQIAxcel Kitが入手可能です(下表参照)。

QIAxcel Kits
QIAxcel Kit 分析サンプル サイズ範囲 100 bp ~ 500 kb* 500 bp ~ 1 kb* 1 bp ~ 5 kb* 5 bp ~ 10 kb* 泳動時間/12サンプル
QIAxcel DNA High Resolution Kit DNA 15 bp ~ 10 kb 3~5 bp 50 bp 200~500 bp 1 ~1.5 kb 7~20分
QIAxcel DNA Screening Kit DNA 15 bp ~ 5 kb 20~50 bp 100 bp 500 bp 5分
QIAxcel RNA QC Kit v2.0 RNA 15 bp ~ 6 kb 200 ~ 500 bpの間で最適な分離能 10分
*最高分離能。ラン時間は使用したメソッドにより異なる。最高分離能は1~3 kb。

Procedure

QIAxcel Advanced System は、ゲルカートリッジのセット、バッファートレイの充填とセット、96 ウェルプレート/PCRチューブ/ストリップチューブにサンプルをアプライ、使用するprocess profile を選択というような簡単なステップで成り立っています。手間のかかるゲル作製や専門的なトレーニングは不要なので、ラボのワークフローが能率化され、ルーチンワークへのインテグレーションが容易に行なえます。泳動開始数分後に、コンピューター画面に最初の結果がリアルタイムで表示されます(図“ラン時間の短縮”)。

Applications

QIAxcel Advanced System は広範なアプリケーションに至適化されているので、基礎研究および薬学、バイオテクノロジー、バイオメディカル研究に最適です。プログラミング済みのメソッドと対応するゲルカートリッジを組み合わせることにより、シングルあるいは複数のPCRフラグメント、制限酵素反応により切断されたDNA、トータルRNA、cRNAなどの様々な核酸の分離と解析を実現します。

Software

QIAxcel ScreenGel softwareはさらなる簡便性を提供

QIAxcel Advanced System用に開発されたQIAxcel ScreenGel Softwareは、データ収集や解析用にデザインされた高性能で使い易いツールです。この画期的なソフトウェアにより、解析とデータ解釈が容易に行なえ、データはゲルおよびエレクトロフェログラムの両フォーマットで表示可能です。結果は個々に表示することも、サンプルおよびデータ比較のために重ねて表示させることもできます。複数データセットの同時解析により評価を単純化できます。ユニークなアルゴリズムが計算を実施し、DNAフラグメントのピーク数、各ピークのサイズ、高さ、幅、面積などを含むピークの特性を示す表を作成します。包括的なデータレポートを容易に作成、保存でき、ニーズに合った個々のドキュメンテーションにエクスポートすることも可能です。サンプル泳動からデータ解析、レポート作製、データエクスポートまでをカバーする包括的なprocess profileでサンプルプロセッシングを標準化することにより、ユーザートレーニングを最小限に抑えることができます(図 "データ収集と解析の簡便化および標準化")。

簡単なセットアップと操作の開始

Process Wizardにより実験を容易に開始できます(図 "使い易いソフトウェア")。 この便利な機能により、セットアップのガイドとランパラメーターの設定ができ、DNA size marker を前もって選択することができます。試薬のロット番号情報も含まれます。マウスを1回クリックするだけでサンプルを選ぶことができ、ランチェックも容易に行なえます。

21 CFR Part 11をサポート

QIAxcel ScreenGel Softwareは21 CFR Part 11の技術要件に準拠しており、電子記録システムの使用が可能です。セキュリティには以下のような特長があります:

  • 不正アクセスやデータ操作を防ぐためにパスワードで保護されたログイン
  • 構成ファイルとシステムイベントの監査証跡ドキュメンテーション
  • 書き込み保護された生データの自動保存とアーカイブ
  • 安全なユーザー管理

様々なユーザープロファイル(Routine、Basic、Advanced、Admin)があり、パスワードにより保護(ユーザーログインが必須)されているので安全です。簡略化されたインターフェイスによりユーザートレーニングの必要性を最小限に抑えられ、経験の浅いユーザーにとってこのソフトウェアは特に有効です。

Services

Are you thinking of upgrading to the latest QIAxcel generation, the QIAxcel Connect?

Then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply trade up your old QIAxcel Advanced to the QIAxcel Connect, or trade in a competitor instrument to the QIAxcel Connect.

Learn more about trade-in/trade-up opportunities.

Resources

キットハンドブック (9)
For calibration of signal intensity
For optimal DNA size determination
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
For optimal RNA fragment size determination
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments
For optimal use of the QIAxcel Gel Cartridges
オペレーティング・ソフトウェア (3)
This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.
This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.
パンフレット (10)
A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher
PCR 実験における重要項目と新技術
A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher
Addressing critical factors and new solutions

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

追加リソース (8)

Method file for use with QIAxcel ScreenGel Software
 
 

Alignment marker file QX15/600 bp - English (XAM)
Method file for use with BioCalculator Software
Method file for use with BioCalculator Software
Method file for use with QIAxcel ScreenGel Software
Method file for use with QIAxcel ScreenGel Software
Process Profile and Method file for use with QIAxcel ScreenGel Software
Method file for use with BioCalculator Software
Application Notes (27)
The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
機器ユーザーマニュアル (2)
For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6
機器テクニカル資料 (1)

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (10)

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Addressing critical factors and new solutions
PCR 実験における重要項目と新技術
A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher
A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher
Additional Resources (8)
Alignment marker file QX15/600 bp - English (XAM)
Method file for use with BioCalculator Software
Method file for use with QIAxcel ScreenGel Software
Method file for use with BioCalculator Software

Method file for use with QIAxcel ScreenGel Software
 
 

Method file for use with QIAxcel ScreenGel Software
Process Profile and Method file for use with QIAxcel ScreenGel Software
Method file for use with BioCalculator Software
Kit Handbooks (9)
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
For optimal DNA size determination
For optimal RNA fragment size determination
For optimal use of the QIAxcel Gel Cartridges
For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
For calibration of signal intensity
Instrument User Manuals (2)
For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6
Operating Software (3)
These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.
This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.
This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
Instrument Technical Documents (1)

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

FAQ

Why does the QIAxcel System or QIAxcel Advanced need to be calibrated?

The QIAxcel System or QIAxcel Advanced works on the basis of fluorescence detection technology. Each channel has a separate excitation light source, with its own optical detection. So each gel cartridge channel is different in the fluorescence emission signal collection mode with its own background and/or baseline noise. When you use a new cartridge, calibration (intensity normalization) is necessary to equalize the variations.

FAQ ID -9019
Why is no signal detected in a lane?

First, check that the glass capillaries at the bottom of the cartridge are intact. You should see approximately 1 mm of glass capillary. Second, check that each tube has at least 10 µl sample volume.

Check the capillary is unblocked by performing the gel-droplet test as outlined in Appendix D in the QIAxcel DNA handbook.

Perform a detector test.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.  
FAQ -9015
Why do I hear a grinding sound during transportation when first installing the QIAxcel System or QIAxcel Advanced?
When the QIAxcel or QIAxcel Advanced is first installed and the Transport Locking assembly is removed, there is a possibility that the Transport lower stop is hitting the bottom. When the Transport Locking assembly is first removed, it is recommended to slowly pull up on the Transport assembly so that the lower stop is not hitting the bottom (be sure that the power is turned off before the Transport Lock is removed).
FAQ ID -9017
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?

Yes, if a different method is used with the QIAxcel System or QIAxcel Advanced, a new reference marker table needs to be created because of different relative migration time.

Please follow the instructions described in the manual and run the DNA marker and the Calibration marker with the new method.

FAQ ID -1857
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

  • Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
  • Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
  • Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
  • Analyze the samples immediately.
FAQ ID -1842
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?

Make sure the Gel Cartridge has been calibrated with the computer currently connected.  If not, please re-calibrate the Gel Cartridge.

Make sure the cartridge has equilibrated to room temperature (20°–25°C [68°– 77°F]) and stood upright in the cartridge stand for 20 min prior to use. Empty the buffer tray.

Wash the buffer tray in warm water and rinse thoroughly with deionized or reverse-osmosis water. Refill the buffer tray. Perform a control run with the alignment marker and bland samples (10 μl QX DNA Dilution Buffer). The marker bands should now appear within the chosen separation time.

FAQ ID -1833
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?

 

Refer to Appendix D of the QIAxcel DNA Handbook for hot water soaking and gel droplet testing.  The handbook can be accessed by going to the Resources tab in the link below:

http://www.qiagen.com/products/catalog/automated-solutions/detection-and-analysis/qiaxcel-dna-kits#

 

 

FAQ ID -1838
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?

Unplug the QIAxcel or QIAxcel Advanced instrument and remove the fuse holder to verify that the fuses are good. If not, please replace the fuses (Cat No. 9241178 Fuse, 4A, 250VAC, QX). If this does not correct the problem, contact QIAGEN Technical Services.

FAQ ID -1851
What should I do if I hear a 'hissing" sound when the QIAxcel System or QIAxcel Advanced is purging?
If you hear a 'hissing" sound when the QIAxcel System or QIAxcel Advanced  is purging, remove the Gel Cartridge and verify that the Cartridge Purge Port label is removed.
FAQ ID -9018
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858
What is the QIAxcel Advanced System?

The revolutionary QIAxcel Advanced System replaces traditional, labor-intensive gel analysis of DNA and RNA.

  • Rapid analysis of up to 96 samples without manual intervention
  • Safety and convenience with ready-to-use gel cartridges
  • Robust results for nucleic acid concentrations as low as 0.1 ng/ul
  • Standardized and accurate analysis with a resolution down to 3-5 bp
  • User-friendly analysis software that supports 21 CFR Part 11 compliance

 

See:  http://www.qiagen.com/Knowledge-and-Support/Videos-and-Virtual-Demos/Assay-Demos/QIAxcelAdvancedDemo/  for a virtual demonstration of the special software, features, and applications of the QIAxcel Advanced System.

 

FAQ ID -2650
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?

The working alignment marker is an indication that the cartridge channel is working well. DNA samples that do not appear in all channels of the QIAxcel System or QIAxcel Advanced may be caused by:

  1. Low sample volume (< 10 ul). The minimum required sample volume is 10 ul. 
  2. Trapped air bubble in the well. The air bubble must be removed. 
  3. High salt concentration in the sample. Dilute the sample with the QX DNA or RNA dilution buffer and rerun the sample.
FAQ ID -1844
Why are bands compacted together on the gel image

Check that the lower and upper alignment markers are detected correctly, and that the threshold is set properly.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.
FAQ ID -9015
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??

Verify that the Cartridge Purge Port label is removed. If it has been removed, verify that the alignment marker and samples are in the appropriate locations in the Buffer Tray and the 96-well Sample Tray used with the QIAxcel System or QIAxcel Advanced?.

FAQ ID -1847
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?

When using this specific marker combination with the QIAxcel System or QIAxcel Advanced, i.e. for STR analysis, the 1.8 kb fragment lies outside of the range of detectable fragments and does not show up on the electropherogram and/or the gel view image.

 

FAQ ID -1835
When should the N2 bottle used with the QIAxcel Advanced System be changed?

N2 cylinders on the QIAxcel Advanced should be changed when one or both low pressure warnings are shown in the "Status Information" panel.

The left panel of the screen in the "Process" environment is called the "Status Information" panel.  

"Pressure 1" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is sufficient for the current sample run; however, the N2 cylinder should be replaced once the run has finished.

"Pressure 2" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is insufficient for the current sample run and the analysis will not be performed. The N2 cylinder should be replaced.

FAQ ID -3001
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

The solutions used with the QIAxcel System or QIAxcel Advanced should not have to be changed for the life of the Gel Cartridge (based on the number of runs).  However, if any contamination is suspected, the Buffer Tray should be cleaned and the buffers should be changed immediately.

FAQ ID -1840
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

  1. Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray. 
  2. Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day. 
  3. Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.
FAQ ID -1823
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

The minimum sample volume required for the QIAxcel System and QIAxcel Advanced is 10 ul to guarantee sample injection in each channel.

 

 

FAQ ID -1852
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

If one of the capillaries of the gel cartridge for the QIAxcel System or QIAxcel Advanced is clogged, the corresponding lane in the gel-view image is typically black.

 

FAQ ID -1836
Why is the buffer tray not moving or making a grinding sound?

When running a QIAxcel Advanced instrument with the BioCalculator software, ensure to use version v3.2.07 or higher. You can download the latest version of the software from:

BioCalculator: http://www.qiagen.com/resources/Download.aspx?id=b68d8727-f0d7-44e5-acc5-2bb6ed50b63d&lang=en&ver=1

ScreenGel: http://www.qiagen.com/Products/Catalog/Automated-Solutions/Detection-and-Analysis/QIAxcel-ScreenGel-Software#resources

FAQ ID -9016
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special considerations?

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

  • Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
  • Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
  • Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
  • Analyze the samples immediately.

 

FAQ ID -9020
Can the QIAxcel System or QIAxcel Advanced be connected to a network?

Yes, the QIAxcel System or QIAxcel Advanced can be connected to a local network.

FAQ ID -1862
Is the QIAxcel Advanced discontinued?
The QIAxcel Advanced has been discontinued as of October 2022. This follows the launch of the QIAxcel Connect instrument, the next generation of the QIAxcel technology. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced including providing associated repair services until December 31, 2029. Please contact your customer care or sales representative if you have any questions about the discontinuation of QIAxcel Advanced.
FAQ ID - 3983

I’m getting the low pressure error on a brand new nitrogen cylinder — why?

The BioCalculator (version 3.2.05) and ScreenGel software have a feature to close the pressure valve after the software is idled for more than 30 minutes. To re-open the pressure valve, execute a command with the software, such as move the buffer tray, unlatch and latch the cartridge; alternatively, you can also exit the software and re-launch it. Also see FAQ ID 3001.

FAQ -9011
What are the common causes of low signal?

The sample DNA concentration might be too low. You can increase sample injection time.

A very strong sample in the same run can cause the threshold to be set too high. As a result, the signal from weaker samples is below this threshold. Try diluting the sample with high concentration with the QX dilution buffer.

High salt concentration in the samples, such as restriction enzyme digested DNA, can interfere with sample injection. Try reducing the salt concentration by diluting such samples with the QX dilution buffer.

If the issue persists, please send data files to QIAGEN Technical Services. See  <a href="/knowledge-and-support/faq/?ID=71818D37-21EF-40B0-BA30-6C8CC8CA82B9"><b>Where are the QIAxcel data stored</a></b>.

FAQ -9013
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?

High or very low salt concentration could lead to the described appearance when running samples on the QIAxcel System and QIAxcel Advanced. Make sure QX DNA Dilution buffer has been used to dilute your samples. Dilute your samples 1:2 in QX DNA Dilution buffer and re-analyze.

FAQ ID -1837