MinElute PCR Purification Kit

低溶出量で最高5 µg までのPCR 産物(70 bp~4 kb)精製用


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MinElute PCR Purification Kit (50)

Cat. No. / ID:  28004

50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
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MinElute PCR Purification Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering


  • 最小限の溶出量
  • 迅速な操作と容易な取り扱い
  • 再現性のある高い回収率
  • ゲル解析をより簡便化するGelPilot Loading Dye付き

Product Details

MinElute PCR Purification Kitは、スピンカラム、バッファー、コレクションチューブで構成され、シリカメンブレンによりPCR 産物(70 bp~4 kb)の精製を実現します。スピンカラムは微量(わずか10 µl)で溶出できるようにデザインされているので、高濃度のDNAが高収量で得られます。オプションのpH指示薬により、DNAがスピンカラムに結合する最適なpHを簡単にチェックできます。本キットはQIAcubeで完全自動化が可能です。


MinElute PCR精製法でDNAサンプルからプライマー、ヌクレオチド、酵素、ミネラルオイル、塩類などの夾雑物を除去することができます(図 “ プライマーの効率的な除去”)。

MinElute PCR Purification Kitには、PCR生成物クリーンアップ用のスピンカラムが含まれています。マイクロ遠心機または吸引マニホールドを使用することにより、高濃度なDNAフラグメント(70 bp~4 kb)が迅速に得られます(4 kb以上のDNAフラグメントの精製には QIAquick PCR Purification Kit をご利用ください)。

See figures


MinElute PCR Purification Kitでは、高塩濃度バッファーによりDNAを結合し、低塩濃度バッファーあるいは水によりDNAを溶出するシリカゲルメンブレンを利用しています。シリカメンブレンテクノロジーにより樹脂漏れ、および懸濁液関連の問題や不便さが解消されます。

Gel Loading Dye

より迅速で簡便なサンプル処理と解析を実現するため、ゲル電気泳動用のローディング色素が付いています。GelPilot Loading Dye は3 種類のマーカー色素(xylene cyanol、bromophenol blueおよびorange G)が入っており、短いDNAフラグメントのゲルからの流出を回避でき、アガロースゲルの泳動時間の至適化が簡単に行なえます(図 " GelPilot Loading Dye")。

See figures


MinEluteシステムでは結合-洗浄-溶出という簡単な操作だけでDNAのクリーンアップが可能です。結合バッファーをPCRサンプルあるいは他の酵素反応液に直接添加し、混合液をMinElute Spin Column にアプライします。ゲル切片をpH指示薬を含んだバッファーに溶解させると、DNA 結合の至適pH を容易に決めることができます (図  “pH指示薬”)。DNAは添付のバッファー中の高塩濃度の条件下でシリカゲルメンブレンに吸着します。不純物は洗い流され、純粋なDNAを添付の低塩濃度溶出バッファー、あるいは水で溶出します。 得られたDNAは、その後のアプリケーションに即使用可能です。


MinElute Spin Column は2つの簡便な操作法オプションのためにデザインされました(フローチャート"MinElute操作手順")。スピンカラムは簡便な卓上型マイクロ遠心機あるいはQIAvac 24 PlusやQIAvac Luer Adapter付きQIAvac 6S (図 "Spin Column操作オプション A B C D、および E")のようなルアーコネクター付き吸引装置で使用でき、QIAcubeでの完全自動化も可能です。

See figures



  • 次世代シークエンシングを含むシークエンシング
  • マイクロアレイ解析
  • ライゲーションやトランスフォーメーション
  • 制限酵素分解
  • 標識反応

Supporting data and figures


MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
クイックスタートプロトコール (1)
キットハンドブック (1)
MinElute Handbook
PDF (611KB)
Certificates of Analysis (1)


Factors involved in root formation in Medicago truncatula.
Imin N; Nizamidin M; Wu T; Rolfe BG;
J Exp Bot; 2006; 58 (3):439-51 2006 Dec 6 PMID:17158109
Expression of c-kit in human osteosarcoma and its relevance as a prognostic marker.
Sulzbacher I; Birner P; Toma C; Wick N; Mazal PR;
J Clin Pathol; 2006; 60 (7):804-7 2006 Oct 3 PMID:17018686
Application of microdroplet PCR for large-scale targeted bisulfite sequencing.
Komori HK; LaMere SA; Torkamani A; Hart GT; Kotsopoulos S; Warner J; Samuels ML; Olson J; Head SR; Ordoukhanian P; Lee PL; Link DR; Salomon DR;
Genome Res; 2011; 21 (10):1738-45 2011 Jul 14 PMID:21757609
Transcriptional organization, regulation and role of the Porphyromonas gingivalis W83 hmu haemin-uptake locus.
Lewis JP; Plata K; Yu F; Rosato A; Anaya C;
Microbiology (Reading); 2006; 152 (Pt 11):3367-3382 2006 Nov PMID:17074906


I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?

CoralLoad dyes supplied in PCR Kits such as, e.g., Taq, HotStarTaq, and TopTaq DNA Polymerase and TopTaq Master Mix do not interfere with most downstream enzymatic applications.

However, for reproducible results, purification of PCR products using the QIAquick or MinElute PCR Purification Kits prior to enzymatic manipulation is recommended.



FAQ ID -1745
What is the composition of Buffer PB?
Buffer PB contains a high concentration of guanidine hydrochloride and isopropanol. The exact composition of Buffer PB is confidential. However, this buffer can be purchased separately: Buffer PB.
FAQ ID -2791
Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?
No - mineral oil will not affect the clean-up procedure with the QIAquick or MinElute PCR Purification Kit.
FAQ ID -575
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
Yes, and therefore they are interchangeable.
FAQ ID -581
Why does my DNA sample float out of the slot when loading it onto an agarose gel?

DNA fragments purified with the QIAGEN DNA Cleanup Systems, i.e., the QIAquick PCR Purification Kit, the MinElute Reaction Cleanup Kit, the QIAEX II Gel Extraction Kit etc. may float out of the loading wells of agarose gels due to residual ethanol carried over from the wash step with Buffer PE (despite the addtition of glycerol-containing loading buffer).

Use either of the following options to remove residual ethanol from the eluate:

  • re-purify the sample using a QIAquick-, or MinElute column, or QIAEX II resin
  • incubate the eluate at 56°C for 10 min to evaporate the ethanol
  • dry down the sample in a vacuum centrifuge, and resuspend the pellet in a small volume of sterile water
FAQ ID -205
Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?

As a rule of thumb, single-stranded DNA binds to silica with approximately half the affinity of a double-stranded DNA fragment of the same length under the buffer conditions used in the QIAquick and MinElute Kits. Even though no systematic experimental data exists, we expect that recovery of ssDNA fragments of approximately 200 nucleotides and below will not be very efficient after cleanup using the QIAquick PCR Purification Kit or MinElute PCR Purification Kit. By comparison, it should be possible to purify fragments longer than 140 nucleotides using the QIAquick Gel Extraction Kit.

Note that recovery of single strand DNA is influenced to some degree also by factors such as base composition and secondary structure. It has to be determined empirically by the researcher if cleanup of single-stranded DNA with QIAquick columns yields satisfactory results.

FAQ ID -759
What is the composition of Buffer EB?

The composition of Buffer EB is:

  • 10 mM Tris-Cl, pH 8.5

Buffer EB is the elution buffer used in the QIAquick PCR, Gel Extraction, Nucleotide Removal Kits, and MinElute Kits for DNA cleanup, and the QIAprep Miniprep Kits for small-scale plasmid purification. The purified DNA can also be eluted in TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0), but the EDTA may inhibit subsequent enzymatic reactions.

FAQ ID -199
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?

Yes, please follow the User-developed protocol 'Forensic post-PCR purification protocol using the MinElute PCR Purification Kit' (ME01).




FAQ ID -1761
What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?

Occasionally, DNA fragments eluted from the silica matrix of QIAquick, MinElute or QIAEX II Kits will contain denatured single-stranded DNA (ssDNA), appearing as a smaller band on an analytical gel. Under certain conditions, chaotropic agents (present in all silica-based DNA purification methods) can denature DNA fragments. This is a rare event that may be influenced by sequence characteristics such as the presence of inverted repeats or A–T-rich stretches.

Because salt and buffering agents promote renaturation of DNA strands, the following tips are recommended:

  • use the eluted DNA to prepare your downstream enzymatic reaction, but omit the enzyme. Incubate the reaction mix at 95°C for 2 minutes to reanneal the ssDNA, and allow the tube to cool slowly to room temperature before adding the enzyme and proceeding
  • alternatively, the DNA can be eluted from the silica-gel membrane or resin in 10 mM Tris buffer containing 10 mM NaCl. However, the salt concentration of the eluate must then be taken into consideration in downstream applications.
FAQ ID -148
Can I buy QIAquick and MinElute columns separately?

The QIAquick Spin Columns (100) (cat. no. 28115) in the QIAquick PCR Purification, Gel Extraction, Nucleotide Removal and PCR & Gel Cleanup kits are also sold separately from the kits.

The MinElute columns in the MinElute PCR Purification, Gel Extraction and Reaction Cleanup kits are not sold separately.

We always provide extra buffers in our kits so you can scale up reactions, add extra washes or allow for spillage.

FAQ ID -2460