Can PyroMark Gold reagents be vortexed?

The therascreen MGMT Pyro Kit is used for quantitative measurement of methylation of four CpG sites in exon 1 of the human MGMT gene. The kit contains primers and reagents for amplification of the genes, plus buffers, primers, and reagents for quantitative methylation detection in real time using Pyrosequencing technology on the PyroMark Q24 system.

The therascreen UGT1A1 Pyro Kit is used for genotyping of allele variant *28 and allele variant *6 of the human UGT1A1 gene. The kit contains primers and reagents for amplification of the genes, plus buffers, primers, and reagents for genotyping in real time using Pyrosequencing technology on the PyroMark Q24 system.

The therascreen NRAS Pyro Kit is a molecular detection kit for identification of mutations and deletions in the NRAS gene. The kit contains primers and reagents for amplification of the NRAS gene plus buffers, primers, and reagents for detection and quantification of mutations in real time using Pyrosequencing technology on the PyroMark Q24 System.

The therascreen EGFR Pyro Kit is a molecular detection kit for identification of mutations and deletions in the EGFR gene. The kit contains enzymes, primers, and reagents for amplification of the EGFR gene plus buffers, primers, and reagents for detection and quantification of mutations in real time using Pyrosequencing technology on the PyroMark Q24 System.

The therascreen BRAF Pyro Kit is a molecular detection kit for the identification of mutations in the BRAF gene. The kit contains primers and reagents for amplification of the BRAF gene plus buffers, primers, and reagents for detection and quantification of mutations in real time using Pyrosequencing technology on the PyroMark Q24 System.

The therascreen KRAS Pyro Kit is a molecular detection kit for identification of mutations and deletions in the KRAS gene. The kit contains primers and reagents for amplification of the KRAS gene plus buffers, primers, and reagents for detection and quantification of mutations in real time using Pyrosequencing technology on the PyroMark Q24 System.

The PyroMark Q96 MD has been discontinued and is no longer supported. The PyroMark Q96 ID was discontinued in 2016 and the instrument is no longer actively sold. Existing instruments will be supported until the end of August 2025. Reagents will continue to be available until the end of August 2025. To future-proof your Pyrosequencing assays, consider upgrading your work to using a PyroMark Q48 Autoprep System, which is actively sold and supported by QIAGEN.

The PyroMark Q24 was discontinued in 2018. The instrument is no longer sold, but existing instruments will be supported until the end of 2026. In addition, reagents will continue to be available until the end of 2026. To future-proof your Pyrosequencing assays, consider upgrading your work to using a PyroMark Q48 Autoprep System which is actively sold and supported by QIAGEN.

Users of PyroMark Q96 ID, the PyroMark Q24 and PyroMark Q24 Advanced and PyroMark Q24 MDx instruments should be aware that reagents for these instruments will be discontinued after August 2025, December 2026 and February 2027, respectively. Assays should be migrated to the PyroMark Q48 Autoprep instrument to improve the Pyrosequencing workflow with automation and advanced methylation, mutation and SNP quantification.