Real-time qPCR

SARS-CoV-2 epidemiology

Reliable genotyping of virus isolates is essential to understand the evolution, virulence and transmission of SARS-CoV-2 and emerging variants. Moreover, during the respiratory virus season, when there is a significant overlap between SARS-CoV-2, flu A/B and RSV A/B symptoms, it is critical to accurately discriminate between the co-circulating viruses by multiplex, real-time PCR. Discover a robust, same-day genotyping approach and a simple, cost-effective multiplexing strategy to match your research goals.

Gene expression analysis

Analyzing gene expression by quantifying the corresponding mRNA or lncRNA is critical to many areas such as cancer and disease research and biomarker identification. For quantification of known targets, reverse transcription qPCR (RT-qPCR) remains the gold standard method, providing fast analysis from just a few to many genes

Our gene expression products enable sensitive quantification with any type of RNA or transcript abundance to deliver unbiased gene expression profiles and get you to your insights faster.

miRNA quantification and profiling

MicroRNAs (miRNAs) have emerged as potential biomarkers for numerous diseases and biological processes, such as cancer, heart disease, nervous system function, hemostasis and DNA repair.

Quantitative PCR is the tool of choice for analyzing known miRNA targets and offers several benefits over NGS. qPCR is fast, simple and inexpensive for most labs to set up and provides extremely sensitive analyses from low amounts of input RNA. It can be easily scaled to various research needs, from screening and profiling, to validation of NGS studies and verification of results from functional studies.

Microbial analysis

Standard PCR kits and reagents can introduce tiny amounts of contaminating nucleic acids into your experiments, jeopardizing results and conclusions.

Designed with microbiome and sensitive microbial applications in mind (e.g., microbial ID, food surveillance, pathogen detection, QC or residual DNA testing, and microbial-host interactions), our new UltraClean Production (UCP) real-time PCR kits bring greater precision to your experiments and confidence to your results.

Detecting microbial contamination

Up to 70% of mammalian cell cultures used to publish work are unknowingly contaminated by bacteria, viruses, or parasites. One company has set the course to help researchers and scientists detect contamination and obtain valid results. Perfectus Biomed has built a microbiology lab from the ground up using QIAGEN PCR technology to ensure valid results, cell line detection, and no contamination.

DNA quantitation

Quantitation of DNA targets plays an important role in many research areas: from detection of genetically modified organisms (GMO) in food to the analysis of genetic variations such as single nucleotide polymorphisms (SNPs), copy number variations (CNV) and somatic mutations.

Our selection of QuantiNova Real-Time PCR Kits help remove the experimental variation and human errors from your qPCR workflow to deliver precise and reproducible quantitation.

Discover the benefits of QuantiNova 

QuantiNova kits represent today’s most advanced portfolio of real-time PCR and RT-PCR kits for gene expression analysis, gene quantification and pathogen detection. A unique combination of in-process controls removes variables and minimizes human error, so your experiments are bound to work. Simple and fast procedures increase your efficiency, saving you time and money. Plus, flexibility in detection chemistry and full real-time cycler compatibility mean there’s no need to compromise.