Guidelines for transfection
miRNA mimic experiments — positive control
miRNA mimic experiments — negative control
miRNA inhibitor experiments — positive control
miRNA inhibitor experiments — negative control
For optimal results, transfection efficiency should be as high as possible. When performing start-up experiments or working with a new cell line, it is necessary to perform multiple transfections under different conditions to determine the optimal conditions for maximum transfection efficiency. Transfection conditions that result in the greatest degree of cell death in comparison to transfection with a negative control can be maintained in future experiments. This control should be performed for optimization and start-up experiments and can be used as a routine transfection control in every experiment.