Penta·His Antibody, BSA-free

For sensitive detection of His-tagged proteins

Detection of C-terminal, N-terminal, and internal His tags
Highly sensitive and specific detection of HHHHH epitopes

Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.

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Cat No./ID: 34660

Penta·His Antibody, BSA-free (100 μg)

HK$8,870.00

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100 μg mouse anti-(H)₅ (lyophilized, BSA-free, for 1000 ml working solution)

The Penta·His Antibody, BSA-free is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

His-tagged thioredoxin detected by Penta·His Antibody in yeast cells.

Identification of positive expression clones by colony blotting.

Immunohistochemistry with Penta·His Antibodies.

Sensitivity of QIAexpress Anti·His Antibodies.

Performance

Performance of Penta·His Antibody BSA-free
Property	Value
Detection:	N-terminal, C-terminal, and internal 6xHis tags
Sensitivity in dot blots:*	0.5 ng
Isotype:	Mouse IgG1
Cross-reactivity:	Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form:	Lyophilized
Working solution:	1000 ml

Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)

Proteins tested with QIAexpress Anti·His Antibodies
Protein	Tag location	Sequence
A DHFR	N-terminus	MRGSHHHHHHGSVRPLNCIV...
B DHFR	C-terminus	...VYEKKDGSRSHHHHHH
C Thioredoxin	N-terminus	MRGSHHHHHHGSSDKIIHLT...
D TNFα	N-terminus	MRHHHHHHQSTESMIRDV...
E TFIIAγ	N-terminus	MGSSHHHHHHSSGLVPRGSH...
F Chaperonin	C-terminus	...AKIAKDGSRSHHHHHH
G DNA Polymerase	C-terminus	...EDWLSAKERSHHHHHH
H TFIIAαβ	Internal	...QQQHQPQQQQHHHHHHQQAQPQQTVP...

For other experiments with Anti·His Antibodies see figures Identification of positive expression clones by colony blotting and His-tagged thioredoxin detected by Penta·His Antibodies in yeast cells

Principle

All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. See figure Immunohistochemistry with Penta·His Antibodies.

Applications

QIAexpress Detection Systems allow efficient detection of His-tagged proteins and are ideal for many applications, including:

Colony, dot, and western blotting procedures
Screening for positive expression clones
Monitoring expression levels and stability of His-tagged proteins
Immunoprecipitation and ELISA

Specifications

Features	Specifications
Detection	Secondary antibody required
Epitope detected	HHHHH
Sensitivity in dot blots (chemiluminescent detection)	10 pg
Sensitivity in dot blots (chromogenic detection)	0.5 ng
Sensitivity in Western blots (chemiluminescent detection)	50 pg
Sensitivity in Western blots (chromogenic detection)	2 ng
Substrate for blot detection	Dependent on secondary antibody
Substrates for assay procedure	Dependent on secondary antibody
Tag	His-tag

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FAQs (9)

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	What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize? FAQ ID -170	View
	Which Anti-His Antibody is the most sensitive for my protein of interest? FAQ ID -172	View
	How should I store Anti·His and Tag·100 Antibodies? FAQ ID -194	View
	What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies? FAQ ID -1093	View
	Are QIAGEN monoclonal Anti His Antibodies glycosylated? FAQ ID -334	View
	What are the dissociation constants for the Anti-His Antibodies? FAQ ID -385	View
	What is the difference between BSA-free and BSA-containing RGS-His Antibody? FAQ ID -569	View
	How should I dissolve the lyophilized Anti-His and Tag Antibodies? -100	View
	How is an antibody epitope determined? FAQ ID -593	View

Kit Handbooks (1)

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	QIAexpress Detection and Assay Handbook - (EN) For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates	Show details

Safety Data Sheets (2)

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	MSDS Penta·His Antibody, BSA-free (100 μg)
	CofA

References

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