Comparison and analytical bridging of digital PCR platforms for AAV titration
Adeno-associated virus (AAV) has emerged as the primary vector for in vivo gene therapy applications. AAV samples are measured and dosed based on DNA concentration, traditionally assessed through conventional quantitative PCR (qPCR). However, digital PCR methods, which allow for absolute quantification of target sequences and eliminate the need for a standard curve, are replacing qPCR methods due to their improved precision. An accurate AAV titration method is crucial throughout the entire drug development process, including preclinical studies, process development, and clinical dosing.
This webinar will cover the following topics:
- Highlights of method development for establishing well-controlled AAV titration methods
- Comparison of multiple digital PCR platforms for AAV titration
- Strategies for transitioning from legacy qPCR methods to enhanced digital PCR platforms