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PAXgene Blood DNA Tubes

For blood collection prior to genomic DNA purification using the PAXgene Blood DNA Kit

Features

  • Storage for up to 14 days at room temperature
  • Easy handling
  • Blood collection and purification in one system
  • Enhanced workflow efficiency
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PAXgene Blood DNA Tubes (100)

Cat. No. / ID: 761115

100 PAXgene Blood DNA Tubes
€183.00
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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The PAXgene Blood DNA system consists of PAXgene Blood DNA Tubes, for blood collection and stabilization, and the PAXgene Blood DNA Kit, for DNA purification in a single-tube procedure.

Performance

DNA isolated using the PAXgene Blood DNA System has an A260/A280 ratio of 1.7–1.9. Fragments are 20–200 kb in size, with an average length of 50–150 kb (see figure " High quality and high molecular weight of genomic DNA"). Average DNA yields are 150–500 µg, depending on the number of nucleated cells present in the blood sample. Purified DNA can be used for PCR and quantitative, real-time PCR (see figure " Efficient multiplex PCR of 3 mitochondrial genes").
See figures

Principle

The PAXgene Blood DNA System is an integrated and standardized system for collection and stabilization of whole blood specimens and isolation of their genomic DNA. The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood DNA Kit.

Blood is collected into PAXgene Blood DNA Tubes, which contain a proprietary blend of reagents that both prevents blood coagulation and stabilizes white blood cells. Blood samples can be stored in the tubes for up to 14 days at room temperature. DNA is isolated from the tubes using appropriate buffers supplied in the PAXgene Blood DNA Kit. Highly pure genomic DNA is obtained, suitable for use in a wide range of downstream applications.

Procedure

The PAXgene Blood DNA procedure is shown in the flowchart " PAXgene Blood DNA procedure". Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.

See figures

Applications

The DNA can be used in a wide range of downstream applications, including:

  • PCR and quantitative, real-time PCR
  • Southern blotting
  • SNP genotyping
  • Pharmacogenomic studies
  • SNP discovery and Validation

Supporting data and figures

Specifications

FeaturesSpecifications
Tube size16 x 100 mm
Draw volume8.5 ml
Label typePaper
Quantity100/box
Closure type/colorBD Hemogard/Blue
Additive/concentrationAdditive 2 ml

Resources

Brochures & Guides (1)
For collection, storage, and transport of whole blood for genomic DNA isolation

 

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (3)
For the collection of whole blood for genomic DNA isolation

 

FAQ

Where can I find additional information for PreAnalytiX PAXgene products?
You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .
FAQ ID - 3515
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?

No. DNA purified with the PAXgene Blood DNA System is free of detectable RNA contamination. In contrast to commercial salting out methods, additional RNase digestion steps are not necessary.

FAQ ID - 3499
How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

See subsequent FAQs for information about storage interval and temperature. Each laboratory should validate shipping procedures.

FAQ ID - 3507
How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

Samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days after blood collection.

FAQ ID - 3508
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?

The PAXgene Blood DNA Tubes can be kept at room temperature for up to 14 days after blood collection. Room temperature is designated as 18–25°C.

FAQ ID - 3492
How should I thaw frozen PAXgene Blood DNA Tubes?

Thaw the PAXgene Blood DNA Tubes in a wire rack at ambient temperature (18–25ºC) for approximately two hours or at up to 37°C in a water bath for approximately 15 minutes. Carefully invert the thawed PAXgene Blood DNA Tubes 10 times.

FAQ ID - 3495
How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?

Blood samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days or at 2–8°C for up to 28 days. For long-term storage, samples should be frozen at –20°C.

FAQ ID - 3490
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?

The typical A260/A280 ratio is from 1.7 to 1.9. The DNA molecules are up to 200 kb in size, with fragments of 50–150 kb predominating.

FAQ ID - 3497
Do I have to use a blood collection set with the PAXgene Blood DNA Tube?

No, a blood collection set is not required to use PAXgene Blood DNA Tubes. Further information on phlebotomy technique with PAXgene Blood DNA Tubes IVD can be found in the product handbook.

FAQ ID - 3484
Can the PAXgene Blood DNA Tubes be stored at 4°C?

Yes, the PAXgene Blood DNA Tubes can be stored at 4°C for up to 28 days after blood collection.

FAQ ID - 3493
Can PAXgene Blood DNA Tubes be stored at higher than room temperature (18–25°C)?

Yes, samples in PAXgene Blood DNA Tubes can be stored at 30°C for up to 3 days after blood collection. However, it is not recommended to store blood samples at temperatures higher than 30°C and storage at high temperatures should not exceed 3 days.

FAQ ID - 3509
What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?

The shelf life of unused PAXgene Blood DNA Tubes is 1 year. The expiration date is printed on the tube label and on the shelf carton. Store PAXgene Blood DNA Tubes at room temperature (18–25°C).

FAQ ID - 3488
What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD?

The typical A260/A280 ratio is from 1.7 to 1.9.

FAQ ID - 3511
What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?

Yield of genomic DNA varies depending on the white blood cell count of the patient blood sample, the extraction method, and the elution volume. However, DNA yield typically ranges between 16–135 µg/ml whole blood. Specifically, DNA yields from magnetic bead-based extraction on the QIAsymphony ranges between 16.9–51.7 µg/ml whole blood, from silica membrane-based extraction on the QIAcube 17.1–45.8 µg/ml whole blood, and from salting-out precipitation 4.9–53.7 µg/ml whole blood.

FAQ ID - 3510
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?

The PAXgene Blood DNA Tubes can be stored at –20ºC for up to 3 months. If tubes are to be kept at temperatures below –20ºC, freeze them first at –20ºC for 24 hours, then transfer them to –70ºC or –80ºC.
To freeze PAXgene Blood DNA Tubes, stand them upright in a wire rack or horizontally in a plastic bag. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack.

FAQ ID - 3491
What is the expected yield from 8.5 ml of blood?

Yield of genomic DNA will vary depending on the cell count from the donor blood sample but average range is from 150 µg to 500 µg.

FAQ ID - 3496
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?

Yes, mitochondrial and genomic DNA are both isolated.

FAQ ID - 3498
Can smaller volumes of blood be processed from the PAXgene Blood DNA Tube?

No. The volume of Buffer BG1 (lysis buffer) prefilled in the processing tubes, and all the other buffer volumes are optimized for use with blood from one PAXgene Blood DNA Tube.

FAQ ID - 3500
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?

To date, DNA purified from blood samples in PAXgene Blood DNA Tubes has been successfully tested for use in PCR, including multiplex PCR.

FAQ ID - 3512
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?

Blood collection using PAXgene Blood DNA Tubes is performed using standard phlebotomy protocol. Blood is collected into the evacuated tube using a needle or blood collection set with a standard needle holder that can accommodate a 13 mm diameter tube. Further information on phlebotomy technique with PAXgene Blood DNA Tubes can be found in the product handbook.

FAQ ID - 3485
How should DNA purified using the PAXgene Blood DNA System be stored?

DNA purified using the PAXgene Blood DNA System should be dissolved in Buffer BG4 and stored at 2–8°C. For long-term storage, DNA can be stored at –20°C but multiple freezing and thawing cycles should be avoided.

FAQ ID - 3502
What is the additive in PAXgene Blood DNA Tubes

PAXgene Blood DNA Tubes contain a proprietary EDTA formulation. Contents of the tube may cause irritation to eyes, respiratory system, and skin. For more information, please refer to the product handbook, download the MSDS, or contact BD Technical Service.

FAQ ID - 3486
What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?

So far, we have shown that DNA purified using the PAXgene Blood DNA System can be used in PCR, including long range and multiplex PCR, restriction digestion and Southern analysis, as well as in various SNP detection procedures including,TaqMan®, LightCycler and Masscode technologies.

See trademarks

FAQ ID - 3506
Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
Yes, the PAXgene Blood DNA Tubes can be stored at 30°C for up to 7 days after blood collection. However it is not recommended to store blood sample at temperatures higher than 30°C
FAQ ID - 3494
Can I use the PAXgene Blood DNA Tube to collect blood from animals?

The system is designed for use only with human blood samples.

FAQ ID - 3504
How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?

See subsequent FAQs for specific storage temperature and time information. Individual laboratories should validate shipping procedures.

FAQ ID - 3489
Are the PAXgene Blood DNA Tubes sterile?

The inside of the PAXgene Blood DNA Tube is sterilized during production.

FAQ ID - 3487