ipsogen BCR-ABL1 mbcr Kit CE

For quantitative detection of BCR-ABL mbcr e1a2 transcripts using real-time PCR

Features

  • Highly accurate and sensitive quantitative assay
  • EAC standardized assay for reproducible results
  • Compliance with EU IVD Directive 98/79/EC
  • Easy workflow with ready-to-use solutions

Useful Resources

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ipsogen BCR-ABL1 mbcr Kit (24) CE

Cat. No. / ID: 670023

For 24 reactions: ABL Control Gene Standards, BCR-ABL mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL mbcr Fusion Gene
The ipsogen BCR-ABL1 mbcr Kit CE is intended for in vitro diagnostic use in Europe.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The ipsogen BCR-ABL1 mbcr Kit is an in vitro molecular diagnostic kit for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts in bone marrow or peripheral blood samples of Ph-positive acute lymphoblastic leukemia (ALL) patients previously diagnosed with a BCR-ABL mbcr fusion gene event. The test is intended to monitor efficacy of treatment in patients undergoing therapy and for minimal residual disease (MRD) follow-up to monitor disease relapse.

Performance

To ensure highest sensitivity, the ipsogen BCR-ABL1 mbcr Kit has been optimized to detect BCR-ABL mbcr p190 e1a2 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of BCR-ABL mbcr plasmid standards and Reliable detection of ABL plasmid standards).

Principle

The ipsogen BCR-ABL1 mbcr Kit is a ready-to-use kit for the detection of BCR-ABL mbcr p190 e1a2 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA extracted from bone marrow or peripheral blood samples of Ph-positive acute lymphoblastic leukemia (ALL) patients previously diagnosed with a BCR-ABL mbcr fusion gene event (see figures BCR-ABL mbcr fusion gene transcript and ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen BCR-ABL1 mbcr Kit provides 5 standard dilutions for BCR-ABL mbcr and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of BCR-ABL mbcr has been standardized according to Europe Against Cancer (EAC) recommendations and the ipsogen BCR-ABL1 mbcr Kit uses this validated technology to calibrate results.

Procedure

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen BCR-ABL1 mbcr Kit allows detection and quantification of BCR-ABL mbcr p190 e1a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen BCR-ABL1 mbcr Kit enable sensitive and reliable detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, for in vitro diagnostic use.

Supporting data and figures

Resources

Kit Handbooks (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.