General Guidelines for Handling DNA

DNA cleanup

Cleanup of DNA is often a prerequisite for efficient downstream applications such as cloning, sequencing, microarray analysis, or amplification.

The use of a dedicated kit for this application may be necessary, since kits can vary depending on the type of reaction and DNA fragment size (e.g., PCR products, gel extraction, enzymatic reactions, nucleotide removal, dye terminator removal) and the required elution volume.

Standard PCR cleanup only requires the removal of ~20 b oligos. In next-generation sequencing (NGS) library preparation, often, much larger primers — almost in the range of a PCR amplicon — must be removed, and PCR cleanup may not be sufficient. For this specialized “size selection” procedures, dedicated kits or PEG-based precipitation (8) are necessary.

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