QIAmini DNA Tissue/Cells Kit

The QIAmini DNA Tissue/Cells (48) Kit contais reagents and single-use prefilled cartridges for the purification of genomic DNA from tissue samples, cultured cells or buccal swabs using QIAmini. This simple protocol is manual lysis followed by hands-free, magnetic-bead-based purification. Following lysis and Proteinase K digestion, you transfer the lysate into the prefilled cartridge, load the cartridge into the QIAmini and start the protocol. Prefilled cartridges minimize pipetting and reduce the risk of human error, leading to reproducible DNA recovery. Purified DNA is suitable for a wide range of downstream applications including qPCR, dPCR and DNA sequencing. The QIAmini DNA Tissue/Cells Kit is designed for laboratories looking for easy-to-use, walk-away and standardized DNA purifications for research.

The QIAmini DNA Tissue/Cells Kit together with QIAmini purifies high-yield DNA from tissue samples (such as brain, liver or spleen), cultured cells (such as Jurkat cells) or buccal swabs. The DNA yields for various tissues purified by QIAmini workflow are similar to platforms from an alternative suppliers and trusted manual spin column kits  Comparable DNA yield from fresh and frozen tissue samples. Performance of DNA from tissues was similar in qPCR  Comparable qPCR amplification from fresh and frozen tissue samples, and performance was slightly better than an alternative supplier in dPCR experiments  Comparable dPCR amplification from tissue samples.

DNA purified from cells using the QIAmini DNA Tissue/Cells Kit had higher yield and performance in qPCR and dPCR when compared to an alternate supplier, and similar yield and performance to a trusted manual spin column kit  Comparable DNA yield, qPCR amplification and dPCR performance from cultured cells.

Finally, QIAmini DNA Tissue/Cells Kit greatly reduces the hands-on time spent performing purifications compared to manual extraction method and processing times for DNA extractions on QIAmini were similar to those of an alternative supplier  Processing time comparison to the QIAmini DNA Tissue/Cells Kit.

The QIAmini DNA Tissue/Cells (48) Kit uses magnetic-bead technology for automated purification of genomic DNA from lysed tissue, cells or buccal swabs. Based on the material, samples are homogenied and lysed before loading into the prefilled cartridge. The prefilled reagent cartridge (with frame) is put in QIAmini. After the protocol is selected, QIAmini automatically performs nucleic acid binding to magnetic beads, wash steps, integrated RNA digestion and elution. The elution volume depends on the sample material used, for tissue and cell samples it is 200 µL and for low biomass samples such as buccal swabs, 60 µL. This cartridge-based protocol simplifies reagent handling and reduces manual pipetting during nucleic acid purification. The purified DNA can be either used immediately in downstream applications or stored for future use.

The simple and intuitive protocol requires only a few preparation and loading steps before fully automated purification begins  QIAmini DNA Tissue/Cells workflow:

1. Prepare samples by lysing tissue, cells or buccal swabs in Buffer ATL followed by Proteinase K digestion. Depending on the sample material, mechanical disruption and homogenization may be recommended.
2. Transfer the lysate together with binding buffer VXL into the prefilled cartridge in well 1 and add elution buffer to well 5.
3. Load the cartridge onto the QIAmini workdeck and start the protocol.*
4. QIAmini performs binding and washing as well as elution of genomic DNA. RNA digestion is optional and be performed off-board.
5. Recover purified DNA from the elution well to a clean tube.

*Protocols must be uploaded to the QIAmini before usage. This is a simple one-time procedure using the barcode scanner, a USB drive or a mobile device with Bluetooth. For a comprehensive list of available protocols see QIAmini Suite.

DNA purified using the QIAmini DNA Tissue/Cells Kit can be used in downstream applications including:

• Quantitative PCR (qPCR)
• Digital PCR (dPCR)
• NGS
• long-read sequencing