QIAmini RNA Tissue/Cells Kit

The QIAmini RNA Tissue/Cells (48) Kit contains reagents and single-use prefilled cartridges for the purification of total RNA and miRNA from tissue and cell samples using QIAmini. This simple protocol consists of manual lysis followed by hands-free, magnetic-bead-based purification. Following lysis and Proteinase K digestion, then you transfer the lysate into the prefilled cartridge, load the cartridge into the QIAmini and start the protocol. Prefilled cartridges minimize pipetting and reduce the risk of human error, leading to reproducible RNA recovery (including small RNAs such as miRNA). Purified RNA is suitable for a wide range of downstream applications including RT-qPCR, dPCR and RNA sequencing. The QIAmini RNA Tissue/Cells Kit is designed for laboratories looking for easy-to-use, compact and standardized RNA purifications for research.

The QIAmini RNA Tissue/Cells Kit together with QIAmini purifies high-yield total RNA from cultured cells and a wide variety of tissue, including fibrous, fatty or RNase-rich tissues. DNA digestion (performed automatically by QIAmini) reliably removes genomic contamination  Efficient genomic DNA removal during RNA extraction. Overall, high-purity, high-yield RNA isolation increases sensitivity of downstream applications. This kit purifies total RNA, including miRNA and other small RNAs  Efficient recovery of miRNA from diverse tissue and cell samples, while greatly reducing the time you spend performing a purification  Processing time comparison to the QIAmini RNA Tissue/Cells Kit.

The RNA quality is comparable whether purified by QIAmini RNA Tissue/Cells Kit and or a trusted manual spin-column kit  High RNA yield and purity across diverse tissue and cell samples.

The QIAmini RNA Tissue/Cells (48) Kit uses magnetic-bead technology for automated purification of RNA and miRNA from lysed tissue and cell samples. Following manual lysis in Buffer RLT and Proteinase K digestion, the lysate is transferred into a prefilled reagent cartridge. Before loading the cartridge into QIAmini, the desired elution volume (50–200 µL; default: 100 µL) is added. The QIAmini automatically performs nucleic acid binding to magnetic beads, wash steps, integrated genomic DNA digestion and elution. The cartridge-based workflow simplifies reagent handling and reduces manual pipetting during nucleic acid purification.

The simple and intuitive protocol requires only a few preparation and loading steps before fully automated purification begins  QIAmini RNA Tissue/Cells Kit workflow:

1. Prepare samples by lysing tissue or cells in Buffer RLT followed by Proteinase K digestion.
2. Transfer the lysate into the prefilled cartridge, add elution buffer and optionally add the DNase for automated genomic DNA digestion.
3. Load the cartridge onto the QIAmini workdeck and start the protocol.*
4. QIAmini performs binding, washing, genomic DNA digestion and elution.
5. Recover purified RNA from the elution well.

*Protocols must be uploaded to the QIAmini before usage. This is a simple one-time procedure using the barcode scanner, a USB drive or a mobile device with Bluetooth. For a comprehensive list of available protocols see QIAmini Suite.

RNA purified using the QIAmini RNA Tissue/Cells Kit can be used in downstream applications including:

• RT-qPCR
• Digital PCR (dPCR)
• RNA sequencing (RNA-seq)
• Gene expression analysis
• miRNA analysis
• Biomarker research