Combating contamination in microbiome and metagenome research
In sensitive microbial and microbiome studies with low biomass samples, standard commercial PCR kits and reagents can introduce tiny quantities of contaminating nucleic acids that affect experimental results and conclusions. This risk is increased with generic priming strategies such as 16S amplification and subsequent sequencing, with added signals from contaminating bacterial DNA.
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The first step in avoiding contamination is to use reagents that don’t include it.
Ultra-clean production enables absolute certainty in your reagents
Manufactured under special conditions to ensure the purity of reagents and components, our UCP kits:
- Eliminate residual DNA and background noise using ultra-clean, nucleic acid-depleted reagents
- Produce reliable, robust results against inhibitors over a wide range of GC content
- Fulfill the needs of NGS workflows and library preparation
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Antibody-mediated hot startPolymerase and exonuclease activity are controlled for superior specificity and sensitivity -
Automation readyIncreased room temperature stability enables automation at room temperature and storage at 4°C -
In-process controlsVisual pipetting control and gel tracking dyes prevent pipetting errors and ensure process success -
Suited for high sample input4x-concentrated master mix formulation enables higher sample input volumes
Our range of UCP PCR Kits is highly suited for microbiome and other sensitive microbial research applications such as pathogen detection, microbial ID, food surveillance, QC or residual DNA testing and studies of host-microbe interactions. UCP will bring greater precision to your experiments and confidence in your results.
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