What is ccfDNA?
Many studies have shown that elevated levels of ccfDNA also known as ctDNA, can be found in the blood of patients suffering from various diseases, especially cancer. ccfDNA differs from normal cell-free DNA by the presence of somatic mutations, which can be used as genetic markers to determine therapeutic response and guide treatment decisions. Levels of mutant ccfDNA can also be useful for following-up cancer patients and detecting minimal residual disease.
How can I overcome the challenges of isolating ccfDNA?
Our QIAamp MinElute ccfDNA and EZ1 ccfDNA Kits use a unique bead and column based method to reliably deliver high-quality material from human plasma or serum for research by providing: efficient recovery of fragmented DNA, scalable sample input volume of 1–10 ml, no organic extraction or ethanol precipitation, effective removal of contaminants and inhibitors.
Can I isolate more than one analyte from one sample?
The QIAamp ccfDNA/RNA Kit offers co-isolation of ccfDNA and ccfRNA from the same plasma sample, providing excellent yield and quality of both analytes in the same eluate.
This allows you to analyze both DNA and RNA mutations in the same liquid biopsy sample or can boost detection sensitivity to discover low-frequency tumor mutations for research.