Deep dive into OEM oligo modifications and bulk manufacturing capabilities
Commercial assay developers face the challenge of ensuring consistent, high-quality components for their products, regardless of the required and ordered volume. At OEM by QIAGEN, we enable our OEM partners to benefit from economies of scale, combined with the knowledge that there is no compromise on the quality of OEM oligonucleotides we provide.
We are capable of incorporating a large selection of modifications in oligonucleotide development and manufacturing, according to GMP guidelines. We are continually expanding our product portfolio with new dyes, quenchers and other modified oligos. For a detailed view of our standard modifications, please review these tables.
Modifications
Fluorophores
| Fluorophores | 5' | Internal | 3' |
|---|---|---|---|
| FAM | ✔ | ✔ | ✔ |
| Fluoresceim dT | ✔ | ✔ | — |
| HEX | ✔ | ✔ | ✔ |
| Quasar® 670 | ✔ | — | — |
| Quasar 670 | ✔ | ✔ | ✔ |
| ROX | ✔ | ✔ | ✔ |
| CAL Fluor® Orange | ✔ | — | — |
| CAL FluorRed | ✔ | — | — |
| Yakima® Yellow | ✔ | — | — |
| TAMRA | ✔ | ✔ | ✔ |
| Chromis 500 | — | — | Inquire |
| Chromis 530 | — | — | Inquire |
| Chromis 645 | — | — | Inquire |
| Chromis 570 | ✔ | ✔ | E |
| Texas Red | ✔ | ✔ | ✔ |
| DY 530 | ✔ | ✔ | ✔ |
| DY 547 | ✔ | ✔ | ✔ |
| DY 495 | ✔ | ✔ | ✔ |
| Cy 3 | ✔ | ✔ | — |
| Cy 3.5 | Inquire | Inquire | — |
| Cy 5 | ✔ | ✔ | — |
| Cy 5.5 | ✔ | ✔ | — |
| Cyanine 5 | ✔ | ✔ | Inquire |
| Cyanine 3 | ✔ | ✔ | Inquire |
| JOE | ✔ | ✔ | ✔ |
Quenchers
| Quenchers | 5' | Internal | 3' |
|---|---|---|---|
| Dabcyl | ✔ | ✔ | ✔ |
| Dabcyl -Succinyl | ✔ | ✔ | — |
| Dabcyl | ✔ | ✔ | ✔ |
| Dabcyl dT | ✔ | — | — |
| Black Hole Quencher® 1 | ✔ | ✔ | ✔ |
| Black Hole Quencher 2 | ✔ | ✔ | ✔ |
| MGB® Eclipse® Dark Quencher | — | — | — |
| Eclipse Dark Quesncher | — | — | Inquire |
| TAMRA | ✔ | ✔ | ✔ |
| Tide Quencher 2 | — | — | Inquire |
| Tide Quencher 3 | — | — | Inquire |
| Tide Quencher 4 | — | — | Inquire |
| BBQ 650 | Inquire | Inquire | ✔ |
Modifiers
Modified bases
| Modified bases | 5' | Internal | 3' |
|---|---|---|---|
| Methyl dC | ✔ | ✔ | — |
| Inosine | ✔ | ✔ | — |
| Inverted dG (dG-5') | — | — | Inquire |
| Inverted dT (dT-5') | — | — | ✔ |
| Inverted dA (dA-5') | ✔ | ✔ | — |
| 2'-OMe-RNA | ✔ | ✔ | — |
Tm Modifiers
| Tm Modifiers | 5' | Internal | 3' |
|---|---|---|---|
| MGB | Inquire | Inquire | Inquire |
| Super A® | ✔ | ✔ | — |
| Super T® | ✔ | ✔ | — |
| Super G® | ✔ | ✔ | — |
| pdU | ✔ | ✔ | — |
| pdC | ✔ | ✔ | — |
Amino
| Amino | 5' | Internal | 3' |
|---|---|---|---|
| Amino ON | — | — | ✔ |
| PT-Aminomodifier C6 | — | — | ✔ |
| MMT-Aminomodifier C6 | ✔ | — | — |
| MMT-Aminomodifier C12 | ✔ | — | — |
| Aminomodifier C6 dT | ✔ | ✔ | ✔ |
| TFA-Pentylaminolinker | ✔ | — | — |
| Aminomodifier C6 dC | ✔ | ✔ | Inquire |
Biotin
| Biotin | 5' | Internal | 3' |
|---|---|---|---|
| Biotin C3 | — | — | ✔ |
| 5'-Biotin | ✔ | — | — |
| Biotin-TEG | ✔ | — | ✔ |
| Biotin-ON | ✔ | ✔ | ✔ |
Thiol Modifier
| Thiol Modifier | 5' | Internal | 3' |
|---|---|---|---|
| Thiol Modifier S-S | Inquire | Inquire | Inquire |
Phosphate
| Phosphate | 5' | Internal | 3' |
|---|---|---|---|
| Phosphorylation | ✔ | — | ✔ |
Phosphorothiate linker
| Phosphorothiate linker | 5' | Internal | 3' |
|---|---|---|---|
| Phosphorothiate bonds | — | Inquire | — |
Spacers
| Spacers | 5' | Internal | 3' |
|---|---|---|---|
| Spacer C12 | ✔ | ✔ | — |
| Spacer 18 (HEG) | ✔ | ✔ | — |
| Spacer C3 | — | — | Inquire |
| Spacer C6 | — | — | ✔ |
| PEO | ✔ | ✔ | — |
Dual-labeled probes
SCORPIONS
Additional OEM Capabilities
| Formulation capabilities | QC support* | Consulting service |
|---|---|---|
|
•Concentration targeting •Lyophilized or in the buffer of your choice •Bulk or aliquots in tubes •Custom mixing •Custom labeling on the product vial |
•Reversed Phase HPLC (RP-HPLC) •Reversed Phase UPLC (RP-UPLC) •Anion Exchange HPLC (IEX-HPLC) •ESI-TOF Mass Spectrometry •Amino reactivity determination |
•Implement tailored modifications and design •Manufacturing performance analysis •Modification evaluation •Sequence design support •Chemical stability studies •Process and result documentation |
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