Cat. No. / ID: 339325
miRCURY LNA miRNA Focus PCR Panels enable exceptionally sensitive and specific miRNA profiling with the miRCURY LNA miRNA PCR System. These PCR primer panels are enhanced with LNA technology and are specific for particular sample types, diseases or pathways, providing a flexible solution that allows you to focus on the miRNAs relevant to your application. miRCURY LNA miRNA Focus PCR Panels are pre-aliquoted in 96- or 384-well PCR plates and are ready for use.
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Why study miRNAs in serum and plasma?
miRNAs are present in small amounts in serum and plasma. They circulate in the blood in a highly stable, cell-free form, suggesting that they are protected from the RNA-degrading enzymes found in blood (see figures miRNAs are stable in serum and plasma and Complete miRNA serum profile).
miRNA profiles have been shown to be altered in serum and plasma taken from patients suffering from cancer and other diseases. This makes miRNAs promising candidates for use in non-invasive discovery of important new biomarkers for a wide range of diseases and biological processes. Blood samples are easy to obtain and carry little risk to the patient, compared with biopsies and surgical procedures.
Challenges of miRNA profiling in serum and plasma
Serum and plasma only contain a fraction of the RNA found in whole blood (see figure Blood serum and plasma contain small amounts of RNA). Most of the RNA is found in the buffy coat, which mainly consists of white blood cells and platelets. To get accurate miRNA profiles, it is important to ensure that the serum/plasma is not contaminated by RNA from blood cells, as this would skew the results of the experiment.
Several other challenges exist when profiling miRNAs in serum and plasma, including:
Overcoming these challenges requires an extremely accurate miRNA qPCR system. The miRCURY LNA miRNA PCR system has a proven track record for reliable detection of miRNAs in serum and plasma samples. >
miRNA and cancer
Numerous studies have demonstrated links between miRNA dysregulation and various disease states, including a diverse array of human cancers. miRNAs have been shown to act as both oncogenes and tumor suppressors. The high relative stability of miRNA in common clinical source materials (e.g., FFPE blocks, plasma, urine, saliva, etc.) and the ability of miRNAs to function as molecular markers for discrete tissue types and disease states have positioned miRNA quantification as a promising new tool for a wide range of applications. miRNAs can be used as disease classifiers for different types of cancers or as biomarkers to monitor the effects of therapy on cancer prognosis and progression.
Identify miRNA biomarkers or new classifiers
Cancer Focus miRNA PCR Panels can be used for many types of cancer miRNA profiling from samples including FFPE and biofluids. These miRNA profiles can be used to identify unique cancer signatures and help identify biomarkers or classifiers for cancer types and progression (see figures Six types of cancers compared and miRNA expression from four types of cancer). >
How the assays were selected
All miRNA assays in the Exosome Focus miRNA PCR Panels were selected based on a combination of experimental data from in-house studies on exosomes using our miRCURY LNA miRNA PCR System and results published in peer-reviewed journals. The assays are not a complete list targeting all miRNAs found in exosomes, but they target those miRNAs typically found (see figure Urine and CSF miRNA profiles). If missing some miRNAs is a concern, we recommend running a full miRNome study using the miRCURY LNA miRNA miRNome PCR Panels.