Kit is a ready-to-use kit for the detection of Wilm's tumor (WT1) gene transcripts using real-time PCR. The kit is based on the amplification and quantification of specific Wilm's tumor (WT1) gene transcripts, relative to ABL control gene expression, in total RNA isolated from patients with acute myeloid leukemia (AML). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen
Kit includes specific plasmids and primers and probe mixes for WT1 and ABL (see figures WT1 gene transcript
and ABL control gene transcript
). Included are 5 standard dilutions for Wilm's tumor (WT1) and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. These components have been validated together in the context of a collaborative study led by a group of experts from the European Leukemia Net (ELN) consortium.