Selection Guide

Choose from a wide range of REPLI-g Kits

Dedicated buffers and reagents have been specifically developed to deliver high yields of DNA from a wide range of sample types, including single cells, limited tissue material, FFPE samples, mitochondrial DNA and purified DNA, with complete sequence representation and unbiased amplification.

REPLI-g Kits
REPLI-g Kit specifications
 Kit Application Starting material Kit output Yield
(µg/reaction)
Reaction
time (h)
Hands-on
time (min) 
REPLI-g Single
Cell Kit
 
Single-cell whole
genome amplification
Single cells,
1–1000 cells,
tissue, purified
gDNA (1–10 ng)
Whole genome
amplified DNA for
analysis by cGH array,
microarray, qPCR or
NGS with the highest
sensitivity
40 µg
8–16 h
 15 min
REPLI-g WTA
Single Cell Kit
Single-cell whole
transcriptome
amplification
Single cells
(mammalian),
1–1000 cells,
purified RNA
(10 pg – 100 ng)
Whole transcriptome
amplified cDNA for
subsequent analysis by
microarray, qPCR or NGS
with the highest sensitivity
20 µg cDNA
4 h
 20 min
REPLI-g Cell
WGA & WTA Kit
Parrallel whole
genome and
whole transcriptome
amplification
starting with a
few cells
25–1000
mammalian cells
Whole genome
amplified DNA
and whole transcriptome
amplified cDNA
Approx. 20 µg cDNA,
approx. 20 µg DNA
4 h
 20 min
REPLI-g Mini Kit Small-sample
whole genome
amplification
    10–40 µg
10–16 h
 15 min
REPLI-g UltraFast
Mini Kit
Small-sample
whole genome
amplification,
with a focus on
short time-to-result
  Whole genome
amplified DNA for
subsequent analysis by
microarray, cGH array,
qPCR, Sanger sequencing
or NGS
7–10 µg
1.5 h
 15 min
REPLI-g Midi Kit Small-sample
whole genome
amplification,
with a focus on
high yield
>10 ng gDNA,
0.5 µl blood or
cells (600 cells/µl)
  40 µg
8–16 h
 15 min
REPLI-g Screening Kit Small-sample
whole genome
amplification,
with a focus on
high sample
throughput
    8 µg
12–16 h
 15 min
REPLI-g FFPE Kit Whole genome
amplification of
DNA from
formalin-fixed,
paraffin-embedded
(FFPE) tissue
FFPE tissue
section (1 cm diameter,
10–10 µm thick);
100 ng gDNA from
FFPE tissue
  Standard yield:
≤10; high-yield: ≤40
Standard yield: 4 h;
high yield: 10 h
 40 min
REPLI-g Mitochondrial
DNA Kit
Whole genome
amplification of
mitochondrial DNA
>1 ng purified gDNA   8 µg
8 h
 15 min

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