RNeasy PowerMax Soil Pro Kit – Soil Total RNA Isolation

For isolation of microbial RNA from large volumes of soil samples including compost, sediment, and manure

S_1321_3_LS_QF_RNeasy_PowerMax_Pro_Soil_Kit_10

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

RNeasy PowerMax Soil Pro Kit (10)

Cat. No. / ID:  47082

For isolation of microbial RNA from large quantities of soil
400,00 $
Log in To see your account pricing.
The RNeasy PowerMax Soil Pro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Uses second-generation Inhibitor Removal Technology® (IRT)
  • Efficient bead beating and lysis chemistry ensures high RNA yields from large sample quantities
  • Suitable for soil types rich in humic acid, including compost, sediment, and manure
  • Ready-to-use RNA suitable for downstream applications such as RT-PCR, qPCR, and next-gen sequencing

Product Details

The RNeasy PowerMax Soil Pro Kit offers a unique and efficient method for isolating total RNA from challenging soil samples, particularly those rich in PCR inhibitors. Using the advanced Inhibitor Removal Technology® (IRT) from QIAGEN, this kit is tailored for samples with high humic acid content. The result? High-quality RNA primed for downstream applications.

Performance

Use of the RNeasy PowerMax Soil Pro Kit results in high-yield, high-purity RNA from diverse soil sources (see figures  RNA Yields – Qubit Quantifications,  RNA Purity 260/280 Ratios). The kit also includes IRT technology for removal of PCR inhibitors (see figure  Inhibitor Sensitive RT-qPCR). Furthermore, the RNeasy PowerMax Soil Pro Kit results in high-quality RNA from various soil sources (see figures  RIN Values). 

See figures

Principle

Soil samples undergo mechanical and chemical disruption to release microbial RNA. The patent-pending IRT technology works to remove PCR inhibitors commonly found in soils, ensuring purified RNA. The RNA is then processed and eluted, ready for downstream applications.

Procedure

Begin with 5–15 g of soil. Each sample undergoes homogenization in a 50 mL bead-beating tube, followed by microbial cell lysis. The addition of IRT removes inhibitory substances. Lysates are processed through the MB Maxi Spin Column where DNA is eliminated, and RNA is purified. After a series of washes, RNA is eluted in RNase-free water, ready for any downstream use.


Sample Storage and Preservation: It's crucial to preserve soil samples promptly post-collection to maintain RNA integrity. Efficient disruption and homogenization are critical to successful nucleic acid purification. Purified samples should be stored at –90 to –65°C.

 

Applications

The purified RNA from the RNeasy PowerMax Soil Pro Kit can be used in diverse applications including RT-PCR, qPCR, and advanced techniques such as RNAseq (metatranscriptome) analysis. 

Supporting data and figures

Specifications

FeaturesSpecifications
Time per run or per prep2 h for 8 samples
Sample size5–15 g
Throughput1–8 samples
Sample typesAll soil samples including compost, sediment and manure
ProcessingBead beating
FormatMaxi silica spin column
Storage temperatureStore at room temperature (15–30°C) and 2–8°C (CD2, RDD and DNase I)

Resources

Quick-Start Protocols (1)
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)