Investigator IDplex Plus Kit

Für die Multiplex-Amplifikation von 13 CODIS-Kernloci sowie D2S1338, D19S433 und Amelogenin, auch direkt aus Abstrichen und Papieren

S_1084_5_GEN_V2

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Investigator IDplex Plus Kit (100)

Cat. No. / ID:  381625

Primermischung, Schnellreaktionsgemisch einschließlich HotStarTaq Plus DNA-Polymerase, Kontroll-DNA, Allelleiter IDplex Plus, DNA-Größenstandard 550 (BTO) und nukleasefreies Wasser
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KitAccessories
Investigator IDplex Plus
Matrix Standard
Reactions
100
400
Dieses Produkt enthält Stoffe, die unter REACH (EG 1907/2006 Anhang XIV) geregelt sind. Die Verwendung dieses Produkts in der EU ist im Rahmen einer Ausnahmeregelung zulässig (Artikel 56(3)). Weitere Informationen finden Sie in der REACH-Meldung und im Sicherheitsdatenblatt zu diesem Produkt, die beide im Abschnitt „Ressourcen“ dieser Seite zu finden sind.
Investigator IDplex Kits sind für molekularbiologische Anwendungen in den Bereichen Forensik, Personenidentifizierung und Vaterschaftstests bestimmt. Diese Produkte sind nicht zur Diagnose, Prävention oder Behandlung einer Erkrankung vorgesehen. Diese Produkte sind nicht in allen Ländern verfügbar.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Gleichzeitige Amplifikation von 16 Loci einschließlich CODIS-Kern
  • Hervorragend geeignet für die forensische Fallarbeit und Referenzproben
  • Unkomplizierte Protokolle mit gebrauchsfertigem Fast Reaction Mix
  • Optimiertes Primerdesign zur Reduzierung der Markerüberlappung

Product Details

Das Investigator IDplex Plus Kit ist ein Multiplex-STR-Assay zur Personenidentifizierung, der die 13 CODIS (Combined DNA Index System) Kernloci, D2S1338, D19S433 und Amelogenin abdeckt.

Das Kit beruht auf unserer Fast-Cycling-Technologie, die eine Amplifikation in etwa 90 Minuten gewährleistet. Das Investigator IDplex Plus Kit ist hochempfindlich und liefert schnelle und zuverlässige Ergebnisse aus DNA-Spuren. Es eignet sich für alle forensischen Anwendungen und Vaterschaftstests. Das genetische Analysegerät wird mit BT5 Matrix kalibriert.

Das Investigator IDplex Kit erfüllt die Anforderungen von ISO 18385.

Performance

Mit Hilfe der Fast-Cycling-Technologie liefert das Investigator IDplex Kit zuverlässige Ergebnisse für STR-Assays in ca. 90 Minuten aus extrahierter DNA.

Der Fast Reaction Mix basiert auf der bewährten QIAGEN-Multiplex-PCR-Technologie, die zur Erhöhung der Inhibitortoleranz optimiert wurde. Die Assay-Leistung, die in Gegenwart steigender Konzentrationen von 6 unterschiedlichen Inhibitoren (Huminsäure, Hämatin, Gerbsäure, Indigokarmin, Kalzium und Kollagen) getestet wurde, zeigt eine hohe Sensitivität und eine überragende Inhibitorresistenz. Dies ermöglicht verlässliche, qualitativ hochwertige Ergebnisse von Fallproben.

Das Investigator IDplex Kit wurde mit dem GeneAmp 9700 Thermocycler und dem Applied Biosystems 3500 Genetic Analyzer validiert und evaluiert.

Die empfohlene DNA-Menge unter Standardbedingungen für die Verwendung mit dem Investigator IDplex Plus Kit beträgt 0,5 ng. Interne Validierungen haben ergeben, dass mit 0,2–2 ng DNA robuste und ausgewogene Ergebnisse und mit < 0,1 ng DNA zuverlässige Ergebnisse erhalten werden.

Principle

Das Investigator IDplex Kit nutzt die Fast-Cycling-PCR-Technologie von QIAGEN für die schnelle, gleichzeitige Amplifikation der 13 CODIS (Combined DNA Index System)-Kernloci, D2S1338, D19S433 und Amelogenin. Sorgfältig entworfene Primer verringern die Überlappung von Loci-Allelen und minimieren so die Wahrscheinlichkeit von Fehlinterpretationen.

Procedure

Die 15 polymorphen autosomalen STR-Marker (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, TH01, TPOX und vWA) und das geschlechtsspezifische Amelogenin werden gleichzeitig in einer einzigen PCR amplifiziert und mit einem der in der Tabelle „Farbstoffe und entsprechende Marker des Investigator IDplex Plus Kit“ aufgeführten Farbstoffe markiert.

 

Farbstoffe und entsprechende Marker des Investigator IDplex Plus Kits
Farbstoff Marker
6-FAM Amelogenin TH01 D3S1358 vWA D21S11
BTG TPOX D7S820 D19S433 D5S818 D2S1338
BTY D16S539 CSF1PO D13S317 FGA  
BTR D18S51 D8S1179      

Die amplifizierten Loci werden dann einer Kapillarelektrophorese unterzogen. Die Ergebnisse können mit spezieller Software, wie GeneMapper ID-X, analysiert werden. Weitere Informationen finden sich in der Tabelle „Technische Daten“.

Applications

Investigator IDplex Plus Kits dienen der raschen und zuverlässigen Erstellung von DNA-Profilen aus Blut, Wangenschleimhautabstrichen und forensischen Spuren oder Referenzproben und eignen sich besonders für:

  • Kriminaltechnische Datenbank, forensische Referenz- und Fallproben
  • Vaterschaftstests
  • Bevölkerungsgenetische Studien
  • Sonstige Anwendungen zur Personenidentifizierung

 

Technische Daten
Merkmale Investigator IDplex Plus Kit
DNA-Eingabemenge Bis zu 15 µl
Volumen pro Reaktion 25 µl
Matrix BT5
Fluoreszenzfarbstoffe 6-FAM, BTG, BTY, BTO
Gentechnische Analysegeräte ABI PRISM 3100/3100 Avant, Applied
Biosystems 3130/3130xl oder Applied
Biosystems 3500 Genetic Analyzers
Software für die Auswertung Applied Biosystems GeneMapper ID oder
GeneMapper ID-X

Resources

Brochures & Guides (2)
Advanced sample collection, automation and STR solutions for kinship testing
Kit Handbooks (1)
Quick-Start Protocols (1)
Template Files (3)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Technical Information (3)

The most comprehensive and versatile coverage of current international STR standards for capillary electrophoresis analysis



Quality initiatives for human identity testing and forensics 
Technical Information and Important Notes (1)
Certificates of Analysis (1)

FAQ

Is the Fast Reaction Mix of the Investigator IDplex Plus Kit ready to use?
Yes. The Fast Reaction Mix of the Investigator IDplex Plus Kit contains HotStarTaq Plus enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only Investigator IDplex Plus Primer Mix and template DNA need to be added. Nuclease-free Water should be added to complete the reaction volume.
FAQ ID -3008
What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?

We recommend first adding master mix to the PCR plate and then adding the punch to avoid punches getting lost or mixed up due to electrostatic effects. Furthermore, we strongly recommend spinning down the PCR plate after setup to ensure all punches get fully submerged.

FAQ ID -3096
Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?
Yes. The protocol is generic and has successfully been tested on various commonly used instruments.
FAQ ID -3089
Can the PCR protocol of the Investigator IDplex Plus Kit be run on different PCR endpoint cyclers?
Yes. The protocol of the Investigator IDplex Plus Kit is generic and has been successfully tested on various commonly used instruments.
FAQ ID -3009
What is the maximum DNA input volume that can be used in the Investigator IDplex Plus Kit?
15 µl input volume can be used with the Investigator IDplex Plus Kit.
FAQ ID -3014
Can additional positive controls be used for the Investigator IDplex GO! Kit?

Amplification of Control DNA 9948 is appropriate as a PCR performance check. However, if you have access to reference samples at constant quality (for example, swabs or FTA donated by lab personnel), these samples may be used as additional in process controls.

FAQ ID -3094
Which filter set should be used with Investigator IDplex Plus Kit?
If you are using an ABI PRISM 3100-Avant/3100 or 3130/3130xl Genetic Analyzer, please use Any5Dye, if available. If you are using the Applied Biosystems 3500/3500xL Genetic Analyzer, please use filter set AnyDye. Otherwise, filter set G5 should be used. If you are using an ABI PRISM 310 Genetic Analyzer, filter set G5 should be used.
FAQ ID -3017
Does the Investigator IDplex Plus assay contain an internal control?
No. Unlike some other QIAGEN STR products (e.g., the Investigator Argus Y-12 QS Kit) there is no QS quality sensor included in the Investigator IDplex Plus Kit.
FAQ ID -3011
Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
No. Up to 2 µl can be added on top of a 25 µl reaction.
FAQ ID -3088
Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?
We do not recommend using more than one punch of 1.2 mm diameter for blood or buccal cells on FTA, or 2 µl of a crude buccal swab lysate. To adapt protocols to the type and quality of samples used in your laboratory, and to the CE instrumentation in use, adjust PCR cycle numbers and CE injection conditions.
FAQ ID -3090
Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?
Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards.

 

FAQ ID -3095
What is the optimal sample DNA concentration for the Investigator IDplex Plus Kit?
The optimum sample DNA input range of the Investigator IDplex Plus Kit is between 200–500 pg. However, reliable results can be obtained with less than 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.
FAQ ID -3015
Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?

No. Amplification products of marker FGA have a slightly different size between IDplex Plus and IDplex GO! kits due to a change in the fluorescent dye linker used. Use of the IDplex Plus ladder will frequently cause off ladder calls for FGA alleles.

 

FAQ ID -3093
Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?

Yes. The Fast Reaction Mix contains Taq enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only IDplex GO! Primer Mix and sample needs to be added.

FAQ ID -3087
Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?

Yes. Sample materials used for direct amplification vary with regard to quality and quantity of DNA present, depending on sampling materials, procedures, and storage conditions. In order to obtain the highest possible success rates in routine use, it is essential to run a representative batch of samples for evaluation of optimal conditions. Furthermore, we recommend revisiting this aspect after a certain period of routine use to ensure the evaluated conditions are optimal and re-adjust if necessary.

FAQ ID -3091
Have primer sequences of the Investigator IDplex Plus Kit been changed compared to the former Investigator IDplex assay?
No, the primer site locations of the markers in the Investigator IDplex Plus Kit have not been changed. Only minor changes have been introduced for some markers, e.g., to improve A addition to the PCR products.
FAQ ID -3012
What is the correct matrix to use with Investigator IDplex Plus Kit?
Matrix BT5 should be used with Investigator IDplex Plus Kit.
FAQ ID -3016
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?

The BT5 matrix standard is designed for use with our 5-dye Investigator STR Assays. We do not disclose the containing dyes.

FAQ ID - 3364
Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator IDplex Plus Kit?
Yes. For example, cycle numbers can be increased to 32 or 34 in order to obtain higher signals for very diluted samples. However, please note that this promotes the amplification of nonspecific products or amplification from spurious background DNA.
FAQ ID -3010
What does the Investigator IDplex Plus Kit contain?
The Investigator IDplex Plus Kit contains Primer mix, Fast Reaction Mix, Control DNA allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water.
FAQ ID -3013
Where can I find the template files for the Investigator IDplex Plus Kit?
The template files for the Investigator IDplex Plus Kit for various softwares and analyzers can be downloaded from the Resource tab on the product webpage.
FAQ ID -3019
Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?

No, primer site locations of markers have not been changed. Two additional SNP primers have been added to allow balanced amplification of vWA and D16S539 alleles carrying known primer binding site mutations. The mutations covered were observed in concordance studies in samples of African origin at an elevated level. The labelled FGA forward primer has been slightly changed with regard to the linker of the fluorescent dye. The change does not affect PCR amplification, but causes a slight size shift during capillary electrophoresis. The allelic ladder therefore has been changed accordingly.

FAQ ID -3092
Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit?

For Any5Dye filter set with the Investigator IDplex Plus Kit, please use:

  • Condition Bound: Lower 1.0 – Upper 20
  • Sensitivity: 0.1
  • Quality Value: 0.80
FAQ ID -3018