Wide Variety of Applications using HRM Technology

Versatile technology for a multitude of applications
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HRM is a versatile and cost-effective method that has been shown to be successfully used for a diverse range of applications, as illustrated by various publications. From analysis of SNPs, mutations, insertions, deletions, and genetic variations such as microsatellites and haplotypes, to detection of changes in CpG methylation status, exploit the full potential of HRM for your research. QIAGEN offers a complete solution for HRM analysis, no matter what your application!

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Fast and accurate detection of class IV SNPs
Successful detection of mutations
Reliable pathogen detection
Highly sensitive DNA methylation analysis 

Fast and accurate detection of class IV SNPs
The Type-it HRM PCR Kit provides the ultimate solution for fast and accurate genotyping via HRM analysis. The kit is optimized to even enable successful analysis of genomic loci that are difficult to amplify.

Procedure: HRM analysis with EvaGreen Dye
Template: Wild-type, heterozygous, and mutant DNAs (1 ng each) for SNP rs 2270938
PCR kit: Type-it HRM PCR Kit
Instrument: Rotor-Gene Q
Assay: HRM assay for SNP rs 2270938
Analysis: Rotor-Gene ScreenClust HRM Software 
View data
 
Successful detection of mutations
Gene mutations (insertions and deletions) are readily detected and reliably distinguished using the Type-it HRM PCR Kit, resulting in distinct melting curves.
Procedure: HRM analysis with EvaGreen Dye
Template: Wild-type and mutant DNA sequences in EGFR exon 19
PCR kit: Type-it HRM PCR Kit
Instrument: Rotor-Gene Q 5plex HRM
Assay: HRM assay for EGFR exon 19
Analysis: Rotor-Gene HRM Software and Rotor-Gene HRM ScreenClust HRM Software
View data

Reliable pathogen detection
HRM can be successfully utilized in microbiology applications such as pathogen typing. Using HRM, 8 different bacterial strains were identified, discriminating the samples accurately by analyzing the 16S rRNA gene.
Procedure: HRM analysis with EvaGreen Dye
Template: Bacterial DNA from various strains
PCR kit: Type-it HRM PCR Kit
Instrument: Rotor-Gene Q 5plex HRM
Assay: HRM assay for the 16S rRNA gene
Analysis: Rotor-Gene HRM Software
View data

Highly sensitive DNA methylation analysis
Using the EpiTect HRM PCR Kit and the Rotor-Gene Q, even low percentages of methylated DNA are detected reliably.
Procedure: HRM analysis with EvaGreen Dye
Template: Mixtures of methylated and unmethylated DNA
PCR kit: EpiTect HRM PCR Kit
Instrument: Rotor-Gene Q 5plex HRM
Assay: HRM methylation assay for the APC (adenomatosis polyposis coli) gene promoter region
Analysis: Rotor-Gene HRM Software
View data

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