Microbial detection, Microbiome, Foundation, Sample technologies
Microbes and Microbiome

Microbial detection

Microbial detection and identification methods

Rising numbers of infectious diseases and growing outbreaks of epidemics require us to be more effective in microbial identification, especially for pathogens. However, microbiome samples are very challenging and require high-quality molecular methods for accurate identification of a particular organism, antimicrobial resistance genes or virulence genes in a microbial population. Here, you can explore the latest research and helpful tools for species-level detection in human health, environmental monitoring, or agricultural and industrial applications.

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High sensitivity, targeted NGS variant detection of viral and bacterial pathogens

The COVID 19 pandemic highlighted the need to quickly identify and differentiate pathogenic variants of concern. One strategy is microbial surveillance of patient areas to help circumvent disease outbreaks. Especially probe based NGS is an efficient enrichment solution. This webinar introduces our QIAseq xHYB Viral and Bacterial Panels, which use hybrid capture technology to provide high sensitivity targeted whole genome enrichment for microbial identification and DNA sequencing.

Pathogen detection using the new dPCR Microbial DNA Detection Assays on the QIAcuity

Digital PCR assays are highly valuable identification methods that can detect microbes and their genes to understand their biological functions. Especially hydrolysis probe based assays and digital PCR allow rapid identification of microbial species, antibiotic resistance genes and virulence genes from diverse samples. This webinar introduces the new dPCR Microbial DNA Detection Assay portfolio and explains how to set up a dPCR microbial DNA detection experiment for the correct identification of microbial species.

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Microbial, Microbe, Microbiome, Microbiota, Microbial DNA qPCR, C. difficile, C diff, C. diff, Cdiff, clostridium, artus, QIAsymphony, QS-RGQ, Rotor-Gene Q, RGQ, QS AS, QIAsymphony AS, bacteria, HAI, healthcare-associated infections, infection, infectious disease, MDx, diagnostic, routine , (Illustration, 3D Illustration, MDx)

Antimicrobial resistance Summit: Identifying antimicrobial resistance from Sample to Insight

Antimicrobial resistance (AMR) detection and surveillance is a high priority in healthcare and environmental settings for the safety of both patients and the general public. However, working with samples used for this type of monitoring, such as stool or wastewater, can be challenging. This online summit takes you through a series of biological samples and demonstrates the workflows and characteristics of each.

Wastewater surveillance to push against pandemics
Knowledge about microbes and pathogens in wastewater reservoirs inform public health authorities
Next generation sequencing for deeper insights

NGS determines the nucleic acid sequence that makes up a genome or targeted genomic regions of a microbe without any previous knowledge of its existence. This allows even the identification of novel pathogens. The unbiased nature of NGS also enables the identification of co-infections that other assays might otherwise ignore. Moreover, NGS can be used for pathogen surveillance to detect genetic variants that may be evolving.

Harness the power of hybrid capture technology for NGS based microbial identification:

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Using digital PCR to detect and quantify what goes unseen

As often in nature, the targets with low and rare abundance give you the most interesting insights. However, finding those targets is often a needle in a haystack problem. That’s where digital PCR comes in handy with its high precision and sensitivity. The new dPCR Microbial DNA Detection Assays for the QIAcuity Probe PCR Kit (for DNA targets) or the QIAcuity OneStep Advanced Probe Kit (for RNA or a mix of RNA and DNA targets) allow to identify >680 targets with up to 5 targets per reaction.

For more details on the assay performance, see the Product Profile:

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