Single cell analysis has become a powerful technology for studying small samples and rare cells, and for dissecting complex populations such as heterogeneous tumors. Single cell technology is enabling many new insights into diverse research areas from oncology, immunology and microbiology to neuroscience, stem cells and embryology. 

Researchers are interested in various downstream analyses of an individual single cell but it is challenged by the low amount of DNA within one single cell which is insufficient to drive the reaction of most genomic downstream applications such as microarray, qPCR or NGS analysis. Whole genome amplification (WGA) overcomes the limitation of sample DNA from a single cell enabling multiple downstream applications. Because each single cell sample represents only one genome, it is essential to have a high uniformity of amplification and no loss of loci to ensure reliable and consistent results.

This webinar introduces an updated method for whole genome amplification for single cells using Multiple Displacement Amplification (MDA), the first step in gaining insights from limited samples. Join us to learn more about the new REPLI-g Advanced!
About the speaker
Ruth Klaever, Ph.D., Scientist Product Development
Dr. Ruth Kläever studied Biology (B.Sc.) and Molecular Biomedicine (M.Sc.) at the Universities of Marburg (Germany), Münster (Germany) and Salamanca (Spain). She performed her Ph.D. studies at the Centre of Reproductive Medicine and Andrology in Münster as a member of the joint graduate school CEDAD-IMPRS. Her research focused on epigenetics in male germ cells, and after finishing her Ph.D. in December 2013 she continued her research projects on a postdoctoral position.In August 2014 Dr. Ruth Kläver joined the Product Development department of QIAGEN. She was heading sub-projects in the development of the PyroMark Q48 Autoprep. In addition, she is supporting live cycle management of the PyroMark product portfolio. Since December 2015 she is working in the field of single cell analysis and is responsible for the development of the QIAscout instrument.
Date of recording:Friday, 30 April 2021
Duration:60 minutes
Single Cell Analysis
Next Generation Sequencing
Informatics & Data