Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing editing events can be challenging for both novices and experts. Suboptimal gRNA design and delivery of the CRISPR components into the target cell can severely affect editing efficiency. This, in turn, can make the already laborious process of characterizing the editing event even more time-consuming and inefficient.

To help you detect and validate your gene edits more effectively, we’ve developed an optimized workflow. The new workflow offers everything you need to characterize your editing event faster and easier, from sample preparation to target amplification and analysis.
In this webinar, you’ll learn how to:

  • save time by skipping the purification step and reducing your cell culture time 
  • design PCR and Sanger sequencing primers with ease
  • accurately check technical failures and input quality 
  • verify your editing efficiency with confidence using the new sequencing analysis tool
About the speaker
Dr. Domenica Martorana,
Dr. Domenica Martorana is an R&D scientist working on gene regulation product development at QIAGEN, Hilden, Germany. She studied Molecular and Cellular Biology at the Georg-August University in Göttingen (Germany), where she received her PhD in 2019. Her PhD research focused on the transcriptional regulation of the unfolded protein response in both fungi and higher eukaryotes. Along the way, she has gathered extensive experience in gene editing and gene regulation in different international projects. Since joining QIAGEN in 2019, Dr Martorana has been involved in developing CRISPR- and functional gene regulation-related products.
Date of recording:Wednesday, 28 July 2021
Duration:40 minutes
Biomedical Research
Cancer (other / various)
Tips & Tricks