PCR/qPCR/dPCR
Specific, sensitive and robust PCR with confidence
When performing gene amplification, your experiments’ success can depend on several critical factors, including the throughput, assay sensitivity, robustness of the reaction even with difficult templates, and reliable data normalization. You may also need to overcome the specific challenges associated with the PCR method you are using.
Whichever method you choose, we offer a wealth of optimized solutions for end-point PCR, qPCR and dPCR. Our kits, assays, instruments and accessories help you amplify and detect targets with confidence and streamline your workflow for virtually any application.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
Browse PCR/qPCR/dPCR
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134 Products found
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAcuity Digital PCR System
For nanoplate-based digital PCR applications
QIAcuity OneStep Advanced Probe Kit
For one-step RT-PCR on the QIAcuity digital PCR instruments
QuantiTect PCR Kits
For qPCR and two-step qRT-PCR using SYBR Green I and for qPCR and two-step qRT-PCR and also multiplex qPCR and two-step multiplex qRT-PCR using sequence-specific probes
QuantiTect RT-PCR Kits
For one-step qRT-PCR using SYBR® Green I and one-step qRT-PCR and multiplex qRT-PCR using sequence-specific probes for gene expression analysis
QuantiNova PCR Kits
For highly sensitive, specific and ultrafast probe-based real-time PCR and multiplex PCR, and unparalleled results using SYBR Green-based qPCR
miRCURY LNA Digital PCR Assays
For extremely sensitive and specific miRNA quantification using QIAcuity EvaGreen-based dPCR
QuantiNova RT-PCR Kits
For highly sensitive and specific one-step qRT-PCR and multiplex qRT-PCR using sequence-specific probes or one-step qPCR using SYBR® Green I for gene expression analysis
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
dPCR Microbial DNA Detection Assays
For digital PCR detection of microbial targets, including bacterial, fungal, parasitic, viral, antibiotic resistance or virulence factor genes
QIAcuityDx Digital PCR System for in vitro diagnostics
A single touchpoint, in vitro diagnostic digital PCR (dPCR) system that seamlessly integrates partitioning/processing, PCR and analysis.
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
QIAcuity Cell and Gene Therapy (CGT) dPCR Assays
For vector genome titration and vector copy number determination with superior accuracy, reproducibility and speed on the QIAcuity Digital PCR System
QIAcuity Residual DNA Quantification and Sizing Kits
For quantification and sizing of host cell DNA with higher accuracy and precision using the QIAcuity Digital PCR System
QIAcuity OneStep Advanced EG Kit
For dye-based one-step RT-PCR on the QIAcuity digital PCR instruments
miRCURY LNA dPCR Custom Assays for miRNA
For extremely sensitive and specific miRNA quantification using QIAcuity EvaGreen-based dPCR
Q-Solution Kit
This kit includes Q-Solution and MgCl2 for obtaining better results from PCR reactions with more challenging setups.
QIAcuity Mycoplasma Quant Kit and Standards
For digital PCR quantification and detection of mycoplasma contamination using the QIAcuity Digital PCR System
QIAcuity UCP Probe PCR Kit
Kits from Ultra Clean Production for use with the QIAcuity digital PCR instruments
dPCR Copy Number Variation (CNV) Probe Assays
For locus-specific analysis of copy number variations and alterations by singleplex or multiplex dPCR
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
VeraSeqâ„¢ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
QIAcuity mericon Food Testing Kits
For digital PCR authentication of food and animal feed using QIAcuity
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
VeraSeqâ„¢ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeqâ„¢ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
mericon Ingredient Authentication Assay
For real-time PCR authentication of food and animal feed ingredients
QIAcuity Nanoplates and Accessories
For use with the QIAcuity digital PCR instruments
QIAGEN OneStep RT-PCR Kit
For highly sensitive and specific one-step RT-PCR
QIAGEN Multiplex PCR Kit
For highly specific and sensitive multiplex PCR without optimization requirements
QIAcuity Probe PCR Kit
For use with the QIAcuity digital PCR instruments
QuantiTect Reverse Transcription Kit
For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis
REPLI-g Single Cell Kit
For highly uniform whole genome amplification (WGA) from single cells or limited sample material
HotStarTaq DNA Polymerase
For highly specific amplification with minimal optimization
HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Rotor-Gene Q
For outstanding performance in real-time PCR
QIAquant
For fast and high performance real-time PCR in a 96- or 384-well format
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
QuantiFast Pathogen +IC Kits
For sensitive and reliable detection of viral RNA/DNA and bacterial DNA, including internal control
miRCURY LNA RT Kit
For optimal first-strand cDNA synthesis with the miRCURY LNA miRNA PCR Systems using SYBR Green or hydrolysis probe
miRCURY LNA miRNA PCR Assays
For extremely sensitive and specific miRNA quantification using LNA-optimized, SYBR® Green-based miRNA PCR
RT2 SYBR® Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Taq DNA Polymerase
Taq DNA Polymerase delivers fast PCR set up with minimal optimization for efficient amplification of routine, low-copy, longer or difficult targets
QuantiNova Pathogen +IC Kit
For ultrafast, simultaneous detection of viral RNA/DNA and bacterial DNA, including internal control
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
QIAcuity EvaGreen (EG) PCR Kit
For use with the QIAcuity digital PCR instruments
QuantiTect Primer Assays
For SYBR® Green-based expression analysis of individual genes using bioinformatically-verified assays
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays
QuantiTect Virus Kits
For highly sensitive detection of viral RNA and/or DNA
Omniscript RT Kit
For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
RT2 qPCR Primer Assays
For accurate and reliable gene expression analysis using laboratory-verified assays
REPLI-g Advanced DNA Single Cell Kit
For whole genome amplification (WGA) from single eukaryotic cells, limited samples or purified genomic DNA
Sensiscript RT Kit
For reverse transcription of less than 50 ng RNA per reaction for end-point PCR
Custom RT2 Profiler PCR Arrays
For reliable and sensitive gene expression profiling using laboratory-verified assays
HotStarTaq Plus DNA Polymerase
For fast and highly specific amplification in all applications
miRCURY LNA miRNA Custom PCR Panels
Custom 96- and 384-well plates of LNA-enhanced, SYBR® Green-based miRNA qPCR assays
Certal Residual DNA Detection Kits
For sensitive probe-based detection of residual CHO host cell DNA, including an internal control
dPCR LNA Mutation Assays
For detecting specific DNA sequence mutations related to cancer and oncogenesis
miRCURY LNA miRNA Focus PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
Taq PCR Master Mix Kit
Taq PCR Master Mix Kit is a 2x solution of Taq DNA polymerase, dNTPs, MgCl2 and PCR buffer to simplify your workflow, reduce contamination risk and improve throughput and reproducibility
HotStarTaq Plus Master Mix Kit
For fast and highly specific amplification in all applications
QuantiFast Multiplex PCR Kits
For fast, multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes
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