Epigenomics

There’s more to the story than DNA sequence

The genetic sequence alone does not represent the full picture of gene expression or cellular function. Epigenetic mechanisms, including DNA methylation, can influence gene activity without changing the DNA sequence. DNA methylation is a stable, heritable, covalent modification to DNA, occurring mainly at CpG dinucleotides, but is also found at non-CpG sites. Methylation is associated with normal developmental processes, as well as the changes that are observable during oncogenesis and other pathological processes, such as gene silencing of tumor suppressor or DNA repair genes. Interrogating DNA methylation degree can be challenging in FFPE and liquid biopsy samples, amplifying the need for robust, reliable solutions that work with low-input samples. QIAGEN’s Sample to Insight solutions for epigenetics applications address these challenges to deliver reliable data and powerful insights.

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Events, stories and more

Studying human disorders with highly multiplexed targeted DNA methylation analysis
In this webinar, Dr. Jörg Tost, Director of the Laboratory for Epigenetics and Environment at CEA, Université Paris-Saclay, describes how QIAseq Targeted Methyl Panels are a well-suited and flexible tool for the simultaneous validation of DNA methylation changes in thousands of CpGs in large cohorts of individuals.

Photo copyright F. Rhodes - CEA
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Methylation status reveals the purity of derived cells
Epigenetic regulation across the genome, and methylation patterns in particular, are important modulators of basic biological processes, as well as the switch between health and disease. In this blog post, discover how understanding the methylation status of specialized endothelial cells in the liver may one day lead to a long-term cell therapy for hemophilia.
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Learn more about targeted methylation detection
Discover the benefits of targeted bisulfite sequencing, an efficient, streamlined approach to maximize throughput and minimize cost when analyzing DNA methylation.
View the brochure
Newest publication using our technology
Muhammad, A. J. et al. (2020)

Molecular Analysis of Fetal and Adult Primary Human Liver Sinusoidal Endothelial Cells: A Comparison to Other Endothelial Cells.
READ THE PAPER

Hypomethylation vs. hypermethylation

Targeted methylation sequencing

Fastest workflow with the lowest sample input for methylation analysis

High performance from tissue, FFPE and liquid biopsy samples with just 1–10 ng of DNA input
Go from DNA to data analysis in one day
Integrated analysis workflows for the non-bioinformatics researcher
Flexibility to customize or fully design panels to your project

Are you seeing a true variant or a false positive?

Each bisulfite-treated DNA molecule is tagged with a unique molecular index (UMI) before amplification. During analysis, PCR duplicates and false positives are removed to give an accurate methylation profile.

Can you cover every region, even regions with high GC-content?

Single primer extension technology (SPE) uses only one specific primer plus a universal primer for each amplicon, compared to the traditional 2-primer approach.

This means:

Lower risk of primer dimers and dropouts
Higher uniformity
Higher library complexity
Only a single pool of primers required

Scaling up your targeted panel content? SPE doesn’t require a primer redesign!

From gDNA to methylome insights in one tube and one day

Save precious samples: Low-input volume requirement safeguards limited samples
Save time: Go from genomic DNA to NGS library in a day using the single-tube protocol
Trust results: Highly specific and uniform bisulfite treatment to convert unmethylated cytosines to uracil
Integrated data analysis: Pre-made and customizable bioinformatics workflows for every researcher

Explore our QIAseq Targeted Methyl and Custom Panels.

Our Illumina-compatible panels include:

Human Breast Cancer Panel
Human Colorectal Cancer Panel
Immuno-Oncology Panel
Human T-cell Infiltration Panel

Fully automate your Pyrosequencing

Pyrosequencing integrates sequence detection and quantification and plays a vital role in research applications looking at epigenetic DNA methylation in gene expression regulation. Discover an easy-to-use, automated protocol that prepares single-stranded DNA samples without manual interaction from the user.

AUTOMATE YOUR WORKFLOW

Watch the latest webinars on methylation analysis

Unlock liquid biopsy samples with NGS-based methylation detection from 10 ng DNA
In this new webinar, we introduce a novel, highly sensitive and faster NGS-based approach to targeted methylation detection using the QIAseq Targeted and Custom Methyl Panels.
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Streamlining analysis of targeted NGS methylation data using QIAGEN CLC Genomics Workbench software
In this webinar, we describe additional aspects of our Sample to Insight solution, focusing on the analysis and visualization of targeted methyl panel data using QIAGEN CLC Genomics Workbench software.
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All photos taken prior to COVID-19

Reveal epigenetic mechanisms in difficult samples