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Animal Cell Culture

This section provides useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects). It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols.

Molecular benchguide

Introduction

  • Animal cell cultures
    • Primary cell cultures
    • Finite cell cultures
    • Continuous cell lines

Safety and handling considerations

  • Safety and handling considerations for animal cell culture
    • Legislation and regulatory guidelines
    • Safety considerations and biohazards
    • Handling cell cultures
    • Aseptic technique and minimization of aerosols
    • Laminar flow hoods

Contamination

  • Contamination
    • Microbial contamination
    • Mycoplasmal infection — detection
    • Mycoplasmal infection — eradication
    • Cross-contamination of cell lines

Cell culture conditions

  • Cell culture conditions
    • Media and serum
    • Incubation conditions
    • Cell culture vessel
    • Cell banking
    • Culture instability

Essential protocols for animal cell culture

  • Essential protocols for animal cell culture
    • Maintaining cell cultures
    • Cell thawing
    • Trypsinizing cells
    • Passaging cells
  • Counting cells
    • Cell counting using a hemocytometer
  • Freezing and viability staining of cells
    • Freezing of cells
    • Viability staining

References