Maximizing PCR success
The reproducibility issue – a growing concern affecting many scientists
Publication of reliable and reproducible studies sets the foundation for future scientific endeavors to build upon. However, many scientists cannot reproduce their peers’ results and sometimes even their own results. This irreproducibility crisis wastes valuable time and resources and can damage scientific credibility. Fortunately, you can help tackle the issue and promote high-quality science by using standardized PCR methods.
Producing meaningful results – Minimize PCR errors and variabilities
Avoid handling variations- Multi-step procedures increase variables
- Undetected human errors can have a big downstream impact
Remove biological variables- Contaminating biomolecules alter results
- Sample to sample variations lead to misinterpretation
- Appropriate controls alert you to performance discrepancies
Select appropriate chemistry- Meaningful results require robust and reliable components
- Choose assay formats with foolproof procedures
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