Tips & tricks to help you characterize CRISPR gene edits
CRISPR technology has advanced rapidly and revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. However, the path from gene edit to biological insight can be challenging for both novices and experts. For example, suboptimal gRNA design and delivery of the CRISPR components into the target cell can severely affect editing efficiency. This, in turn, can lead to a long and tedious screening process.
So, what can you do when characterizing gene edits turns out to be more challenging than you expected? This tips & tricks webinar has you covered.
You will learn:
- What to do when you only have a limited amount of cell material and cultivation time
- How to deal with inhibitors
- How to design sensitive and specific assays
- How to deal with long targets