QIAsymphony RGQ

Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs

Unprecedented flexibility
Workflow optimization and utility
Maximized ease of use and convenience
Regulated assays and laboratory-developed tests
Designed for largest range of applications

QIAGEN's premier sample-to-result workflow solution comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and a broad range of ready-to-use assays on the Rotor-Gene Q real-time PCR cycler. QIAGEN continues to expand the QIAsymphony series with new features, assay menus, and workflows.

Explore the virtual demo to learn more about QIAsymphony RGQ.

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Product		Cat. no.	List price:
QIAsymphony RGQ, System QIAsymphony SP, QIAsymphony AS, Rotor-Gene Q 5plex HRM; includes required accessories and consumables, installation, and training; includes 1-year warranty on parts and labor		9001850	Inquire
QIAsymphony Cabinet SP/AS Accessory for correct positioning of the QIAsymphony SP/AS instruments		9020246	Inquire

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The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application.

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There are 2 modes of system operation: integrated or independent.|Probit analysis: CMV (QIAsymphony RGQ system). Analytical sensitivity of the artus CMV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.|Probit analysis: EBV (QIAsymphony RGQ system). Analytical sensitivity of the artus EBV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.|Probit analysis: HBV (QIAsymphony RGQ system). Analytical sensitivity of the artus HBV QS-RGQ Kit with the QIAsymphony RGQ system.|Probit analysis: HCV (QIAsymphony RGQ system). Analytical sensitivity of the artus HCV QS-RGQ Kit with the QIAsymphony RGQ system.|Probit analysis: HI Virus-1 (QIAsymphony RGQ system). Analytical sensitivity of the artus HI Virus-1 QS-RGQ Kit with the QIAsymphony RGQ system.|

Performance

The QIAsymphony RGQ, in combination with artus QS-RGQ Kits, enables highly sensitive and specific detection of:

CMV DNA (see figure "Highly sensitive detection of CMV DNA")
EBV DNA (see figure "Highly sensitive detection of EBV DNA")
HBV DNA (see figure "Highly sensitive detection of HBV DNA")
HIV-1 RNA (see figure "Highly sensitive detection of HIV-1 RNA")
HCV RNA (see figure "Highly sensitive detection of HCV RNA")

Principle

QIAsymphony RGQ can save you time by processing up to 96 samples, from sample to result, in one integrated run. A dedicated range of kits enable open or closed systems for molecular testing.

QIAsymphony RGQ is the integrated system to maximize your efficiency and optimize your workflows, from sample to result. QIAsymphony RGQ synergizes the automated sample preparation and assay setup on the QIAsymphony SP/AS with manual transfer to the trusted Rotor-Gene Q for detection. The QIAsymphony SP automates sample preparation from a wide range of sample types. The QIAsymphony AS delivers highly reliable and reproducible PCR assay setup and is highly suited for laboratories that perform complex PCR-based molecular testing on a day-to-day basis. The QIAsymphony AS enables setup of multiple assays per run or sample and supports artus QS-RGQ products, for a complete CE-IVD compliant workflow, as well as in-house assays. The Rotor-Gene Q enables sensitive, precise real-time PCR analysis with CE-IVD-marked artus QS-RGQ Kits or in-house assays.

Kits for complete CE-IVD compliant workflow on the QIAsymphony RGQ
Kits	Instrument	Method
QIAsymphony DSP Virus/Pathogen Kits	QIAsymphony SP	Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
artus QS-RGQ Kits	QIAsymphony AS	Real-time PCR (multiplex; sample and internal control)

Kits for in-house tests on the QIAsymphony RGQ
Application	Kits	Instrument	Method
Purification	QIAsymphony DSP AXpH DNA Kit	QIAsymphony SP	Purification of DNA from liquid-based cytology media
	QIAsymphony DSP DNA Kits	QIAsymphony SP	Purification of DNA from human whole blood, buffy coat, tissues, FFPE tissues, cultured cells and bacterial cultures, as well as viral DNA from human whole blood
	QIAsymphony DSP Virus/Pathogen Kits	QIAsymphony SP	Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
	QIAsymphony Certal Kits	QIAsymphony SP	Purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations
Assay setup — pathogen detection	QuantiFast Pathogen +IC Kits	QIAsymphony AS	Multiplex real-time PCR and RT-PCR using sequence-specific probes
	QuantiTect Virus Kits	QIAsymphony AS	Highly sensitive detection of multiple viral DNA and RNA targets in the same tube
	QIAGEN OneStep RT-PCR Kit	QIAsymphony AS	Sensitive RT-PCR, with simple setup, using any RNA template
Assay setup — gene expression	Rotor-Gene SYBR^® Green Kits	QIAsymphony AS	Ultrafast, precise real-time PCR and RT-PCR using SYBR^® Green I
	Rotor-Gene Probe Kits	QIAsymphony AS	Ultrafast, precise real-time PCR and RT-PCR using sequence-specific probes
	Rotor-Gene Multiplex Kits	QIAsymphony AS	Ultrafast, precise multiplex real-time PCR and RT-PCR using sequence-specific probes
Assay setup — residual DNA detection	Certal Detection Kits	QIAsymphony AS	Duplex real-time PCR detection of resDNA target and a generic internal control

Procedure

The QIAsymphony RGQ workflow starts with purification of nucleic acids on the QIAsymphony SP instrument. The eluates, containing purified nucleic acids from the sample preparation procedure, are transferred to the QIAsymphony AS module, which performs assay setup. The assays are then manually transferred to the Rotor-Gene Q for PCR and data analysis.

To suit individual workflow requirements, there are 2 modes of system operation: integrated or independent. In the integrated mode, samples processed on the QIAsymphony SP are transferred automatically to the QIAsymphony AS, reducing manual handling steps and documentation. For added flexibility, the QIAsymphony SP and AS can also be operated independently of each other in independent mode. See "QIAsymphony workflows".

Applications

The QIAsymphony RGQ is the most versatile system for all molecular testing needs — today and tomorrow. The QIAsymphony RGQ allows consolidation of commerical assays, as well as in-house–developed tests, on a single platform.

The broad range of applications for open and closed systems includes:

artus transplantation panel (CMV, EBV, HSV, BK virus, and VZV)
artus blood-borne virus panel (HIV, HCV, and HBV)
In-house tests

Brochures & Guides

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	(EN) - QIAsymphony RGQ Success Story	Show details
	(EN) - QIAsymphony RGQ — Pure Performance	Show details

Application Notes

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	(EN) - Clinical evaluation of the artus HCV QS-RGQ Kit	Show details
	(EN) - Evaluation of the artus CMV QS-RGQ assay for automated extraction, assay setup, and detection of CMV DNA in clinical respiratory and urine samples	Show details

FAQs

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	What is the elution volume for all CE-marked artus QS-Rotor-Gene Q assays? FAQ ID -2432	View
	What is the batch size of the QIASymphony Rotor-Gene Q and how many PCR´s can be processed per assay module run? FAQ ID -2433	View
	Is barcode reading supported by QS-RGQ? FAQ ID -2437	View
	There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do? FAQ ID - 3532	View
	The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony? FAQ ID - 3533	View
	Is it possible to process samples on the AS-module while SP is running? FAQ ID -2435	View
	What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP? FAQ ID -1915	View
	Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -1916	View
	How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP? FAQ ID -1917	View
	Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2162	View
	What RNA elution volumes are available with the QIAsymphony RNA Kit? FAQ ID -1943	View
	What are the recommended lysis times for tissue samples on the QIAsymphony SP? FAQ ID -2993	View
	Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP? FAQ ID -2030	View
	Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument? FAQ ID -2027	View
	How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation? FAQ ID -1941	View
	Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run? FAQ ID -2040	View
	What sample types can be processed using the QIAsymphony Virus/Bacteria protocols? FAQ ID -2036	View
	What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901	View
	What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029	View
	What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP? FAQ ID -2028	View
	What are the expected yields of total RNA isolated with the QIAsymphony SP? FAQ ID -1942	View
	How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2157	View
	What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2158	View
	Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921	View
	Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913	View
	What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP? FAQ ID -1918	View
	Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols? FAQ ID -1920	View
	What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -1919	View
	Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument? FAQ ID -2039	View
	Can the QIAsymphony RNA Kit be used to purify RNA from blood? FAQ ID -1945	View
	What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -2990	View
	Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol? FAQ ID -2038	View
	Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2154	View
	What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit? FAQ ID -2925	View
	How should DNA isolated on the QIAsymphony be stored? FAQ ID -2165	View
	What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2156	View
	What sample input volumes can be used on the QIAsymphony SP? FAQ ID -1907	View
	What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP? FAQ ID -1939	View
	How does magnetic-particle carry-over in QIAsymphony eluates need to be handled? FAQ ID -2166	View
	Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035	View
	Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL? FAQ ID -2032	View
	Is there a charge for user training on the QIAsymphony SP System? FAQ ID -1900	View
	How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? FAQ ID -1905	View
	Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2991	View
	Why do I have to use an ACS (Assay Control Set)? FAQ ID -2933	View
	Is the QIAsymphony RNA Kit compatible with stabilized samples? FAQ ID -1944	View
	Does the QIAsymphony SP system generate run reports and/or log files? FAQ ID -1911	View
	Can uneven sample numbers be processed on the QIAsymphony SP module? FAQ ID -1906	View
	Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -2926	View
	Is it possible to modify QIAsymphony protocols? FAQ ID -1903	View
	Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP? FAQ ID -1937	View
	Is sample pretreatment required for purifying RNA on the QIAsymphony SP? FAQ ID -1938	View
	Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2160	View
	How can the QIAsymphony SP system be decontaminated? FAQ ID -1914	View
	What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit? FAQ ID -2034	View
	How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2927	View
	Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)? FAQ ID -1909	View
	What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits? FAQ ID -2037	View
	Do all QIAsymphony DNA Investigator protocols use carrier RNA? FAQ ID -2033	View
	What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2928	View
	Can I program my own protocols for the QIAsymphony SP? FAQ ID -1904	View
	What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP? FAQ ID -2989	View
	How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP? FAQ ID -1940	View
	What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP? FAQ ID -2992	View
	Can protocol runs on the QIAsymphony SP be interrupted and resumed? FAQ ID -1912	View
	How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol? FAQ ID -2159	View
	Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163	View
	What is the range of elution volumes on the QIAsymphony SP? FAQ ID -1908	View
	Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP? FAQ ID -2031	View

Labware Files and Documents

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	(EN) - QIAsymphony SP/AS adapters and tube inserts	Show details
	(EN) - QIAsymphony Labware V5 (ZIP) Labware package SOW-516-0-08 for use with the QIAsymphony SP/AS instruments (software version 5.0)	Show details

Application/Protocol Documents

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	(EN) - Certal Residual RGQ-5-plex-HRM RD72 V2 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - Certal Residual RGQ-5-plex-HRM RS4 V2 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-96 (15+10) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-96 (40+10) 4ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-96 (45+5) 4ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-LC (15+5) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-RGQ (15+10) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-RGQ (20+5) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-RGQ RD72 (20+5) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter spp QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter spp QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter spp QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Cronobacter spp QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Cronobacter spp QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Cronobacter spp QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Listeria spp QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Listeria spp QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Listeria spp QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter triple QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter triple QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter triple QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon L. monocytogenes QIAGEN-PCR-96 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon L. monocytogenes QS-RGQ RD72 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon L. monocytogenes QS-RGQ RS V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Salmonella spp QIAGEN-PCR-96 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Salmonella spp QS-RGQ RD72 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Salmonella spp QS-RGQ RS V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	Settings to run artus QS-RGQ Kits (Rotor-Gene Q software 2.1, or higher)	Show details

Instrument User Manuals

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	(EN) - Rotor-Gene Q Installation Guide For use with Rotor-Gene Q Software version 2.3.4	Show details
	(EN) - Rotor-Gene Q User Manual For Rotor-Gene Q instruments using Rotor-Gene Q Software version 2.3.4	Show details
	Rotor-Gene Q Important Note Guideline for ISO 20836:2020	Show details
	QIAsymphony SP/AS User Manual - Operating the QIAsymphony SP For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details
	QIAsymphony SP/AS General Description For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details

Instrument Technical Documents

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	(EN) - QIAsymphony Cabinet SP/AS Specifications QIAsymphony Cabinet SP/AS documents	Show details
	(EN) - QIAsymphony Cabinet SP/AS User Guide QIAsymphony Cabinet SP/AS document	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator HE (High Efficiency) Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator ADV (Advanced) HE (High Efficiency) Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator ADV (Advanced) Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	(EN) - Differences between QIAsymphony software versions 4.0.2 and 4.0.3 Differences between QIAsymphony software versions	Show details
	Differences between QIAsymphony software versions 4.0.3/4.1 and 5.0.3	Show details
	(EN) - QIAsymphony File Transfer User Guide Software document for QIAsymphony software version 4.0.1	Show details

Protocol Files

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	(EN) - QIAsymphony V4 AS mericon Protocols For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	(EN) - QIAsymphony V4 AS QIAGEN PCR Applications For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	QIAsymphony SP Applications Investigator V5 For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)	Show details
	(EN) - QIAsymphony V4 AS Certal Protocols For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	(EN) - QIAsymphony V4 AS Installation Protocols For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	QIAsymphony V5 SP Applications For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	QIAsymphony SP Bisulfite140_LC_V1 & QIAsymphony Bisulfite140_HC_V1	Show details

Supporting Software and Tools

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	Language Package – French French language package for QIAsymphony software version 5.0.4	Show details
	QIAsymphony Management Console For QIAsymphony software version 5.0.4	Show details

Scientific Posters

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	(EN) - Development of a new platform for fully automated purification of nucleic acids from a broad spectrum of forensic specimens	Show details
	(EN) - Application of a HT magnetic bead-based DNA extraction system to diverse mAb process intermediates	Show details

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