REPLI-g Single Cell DNA Library Kit
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Dedicated buffers and reagents have been specifically developed to deliver high yields of DNA from a wide range of sample types, including single cells, limited tissue material, FFPE samples, mitochondrial DNA and purified DNA, with complete sequence representation and unbiased amplification.

 

REPLI-g Kit specifications
Kit Application  Starting material Kit output  Yield (µg/reaction) Reaction time (h) Hands-on time (min)
REPLI-g Single Cell RNA Library Kit
Single-cell gene expression analysis by RNAseq
Single cells (mammalian),
1–1000 cells, purified RNA (50 pg–100 ng)



PCR-free NGS library for NGS on Illumina instruments

           
2–4 nM library
7 h for NGS-ready RNA library

<60 min
REPLI-g Single Cell DNA Library Kit
Single-cell DNAseq (genome sequence, SNV, CNV, genomic rearrangements)
 Single cells,
1–1000 cells, purified gDNA (10 pg–10 ng)
2–4 nM library
5.5–6 h for NGS-ready DNA library
<60 min
REPLI-g Single Cell Kit
Single-cell whole genome amplification
 Single cells,
1–1000 cells, tissue, purified gDNA (1–10 ng)
Whole genome amplified DNA for analysis by cGH array, microarray, qPCR or NGS with the highest sensitivity
40 µg
8–16 h
15 min
REPLI-g WTA Single Cell Kit
Single-cell whole transcriptome amplification
Single cells (mammalian),
1–1000 cells, purified RNA (10 pg – 100 ng)
Whole transcriptome amplified cDNA for subsequent analysis by microarray, qPCR or NGS with the highest sensitivity
20 µg cDNA
4 h
20 min
REPLI-g Cell WGA & WTA Kit
Parrallel whole genome and whole transcriptome amplification starting with a few cells
25–1000 mammalian cells
Whole genome amplified DNA and whole transcriptome amplified cDNA
Approx. 20 µg cDNA, approx. 20 µg DNA
4 h
20 min
REPLI-g Mini Kit
Small-sample whole genome amplification




>10 ng gDNA, 0.5 µl blood or cells (600 cells/µl)









Whole genome amplified DNA for subsequent analysis by microarray, cGH array, qPCR, Sanger sequencing or NGS





 10–40 µg
10–16 h
15 min
REPLI-g UltraFast Mini Kit
Small-sample whole genome amplification, with a focus on short time-to-result
7–10 µg
1.5 h
15 min
REPLI-g Midi Kit
Small-sample whole genome amplification, with a focus on high yield
40 µg
8–16 h
15 min
REPLI-g Screening Kit
Small-sample whole genome amplification, with a focus on high sample throughput
8 µg
12–16 h
15 min
REPLI-g FFPE Kit
Whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
FFPE tissue section (1 cm diameter, 10–10 µm thick); 100 ng gDNA from FFPE tissue
Standard yield: ≤10; high-yield: ≤40
Standard yield: 4 h;
high yield: 10 h
40 min
REPLI-g Mitochondrial DNA Kit
Whole genome amplification of mitochondrial DNA
>1 ng purified gDNA
8 µg
8 h
15 min


Top products
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REPLI-g Single Cell DNA Library Kit
For DNA library construction from single cells for Illumina sequencing applications
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REPLI-g Single Cell RNA Library Kit
For RNA library construction from single cells for Illumina sequencing applications
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For parallel whole genome and whole transcriptome amplification from cells and limited samples
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For whole transcriptome amplification of total RNA or mRNA from single cells
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For highly uniform whole genome amplification and quality assessment from small or precious samples
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REPLI-g Single Cell Kit (24)
For highly uniform whole genome amplification (WGA) from single cells or limited sample material
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For highly uniform whole genome amplification from small or precious samples
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For highly uniform whole genome amplification from small or precious samples
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For ultrafast highly uniform whole genome amplification from small or precious samples
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For high-throughput manual or automated whole genome amplification from small or precious samples
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REPLI-g Mitochondrial DNA Kit
For highly uniform whole genome amplification from human and non-human mitochondrial DNA
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For direct whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
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Kit Handbooks
9

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For whole genome amplification from single cells, limited samples, or purified genomic DNA
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For whole transcriptome amplification of total RNA from single cells
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For parallel whole genome amplification and whole transcriptome amplification from cells and limited samples
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For whole genome amplification from purified genomic DNA, blood, and cells
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For fast whole genome amplification from purified genomic DNA, blood, and cells
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For whole genome amplification from purified genomic DNA, blood, and cells
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For direct whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
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