Managing instrument methods in PyroMark Q24 MDx Software
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The PyroMark Q24 MDx dispenses the correct volume of enzyme mix, substrate mix, and nucleotides using variable pulse times and dispensing pressures. The settings for these pulse times and dispensing pressures are specific for each lot of PyroMark Q24 Cartridges and correspond to the method number printed on the PyroMark Q24 Cartridge. The instrument method should be selected in the PyroMark Q24 MDx Software according to the reagents and reagent cartridge that will be used for the run. The settings and instrument method files provided on this page are only for use with PyroMark Q24 MDx Software and PyroMark Gold Q24 Reagents.
Import an instrument method file in PyroMark Q24 MDx Software
Manually create a new instrument method file in PyroMark Q24 MDx Software
Download PyroMark Q24 MDx instrument method files
Important: The settings and instrument method files provided on this page should only be used for Pyrosequencing analyses set up with PyroMark Q24 MDx Software and PyroMark Gold Q24 Reagents.

Import a downloadable instrument method file in PyroMark Q24 MDx Software
  1. Access the available downloadable instrument method files below or under the "Resources" tab on the PyroMark Q24 MDx product page.
  2. Click the instrument method file that corresponds to the method number printed on the label of the cartridge to be used.
  3. Save the instrument method file to the computer running PyroMark Q24 MDx Software.
  4. In the PyroMark Q24 MDx Software, open the "Instrument Method" dialog box from the "Tools" menu.
  5. Click the "Import" button and locate the downloaded instrument method file.
  6. The downloaded instrument method file will now appear in the "Instrument Methods" dialog box under "Methods provided by user" and can be selected in the dropdown list when creating a new Run Setup.
Manually create new instrument method files in PyroMark Q24 MDx Software
  1. Open the "Instrument Method" dialog box from the "Tools" menu.
  2. Click an existing instrument method, e.g., "PyroMark Q24 Method 001 Rev. A created 2008-04-23" which is already implemented in the PyroMark Q24 MDx Software 2.0.
  3. In the table below, find the dispensing pressure and pulse time settings that correspond to the method number printed on the label of the cartridge to be used.
  4. Enter the dispensing pressures and pulse times in the corresponding fields of the "Instrument Method" dialog box.
    Optional: enter information in the notes field, such as file name and date.
  5. Click "Save As" and save the method with a new name (e.g., PyroMark Q24 MDx Method 0003).
  6. When creating a new Run Setup, select this new method (e.g., PyroMark Q24 MDx Method 0003) from the instrument method dropdown list.
Instrument methods for various lots of PyroMark Q24 Cartridges (cat. no. 979302) for the PyroMark Q24 MDx instrument
   Dispensing pressures (mbar)  Pulse times (ms)
 Method  Nucleotides  Reagents  Enzyme mix  Substrate mix  Nucleotides
 0001  625  400  49  45  4.4
 0002  625  400  55  50  4.4
 0003  625  400  63  57  4.4
 0004  625  400  63  57  4.2
 0005  625  400  62  57  4.3
 0006  625  400  62  57  4.5
 0007  625  400  62  57  4.0
 0008  625  400  57  49  4.5
 0009  625  400  57  49  4.0
 0010  625  400  60  54  4.0
 0011  625  400  62  57  4.5
 0012  625  400  62  57  4.5
 0013  625  400  62  55  4.5
 0014  625  400  62  56  4.5
 0015  625  400  60  54  4.5

 

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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0015
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0014
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0011
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0010
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0009
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0008
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0007
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0006
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0005
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0004
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0003
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0002
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Instrument methods file for use with PyroMark Q24 MDx Software, PyroMark Gold Q24 Reagents, and PyroMark Q24 Cartridges marked with method code 0001
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