Single cell analysis is getting into the focus of many research fields as it demonstrates the individual contribution of every cell in a heterogeneous population without obscuring a biological response that may otherwise occur when cells are assessed in bulk. Although single-cell research is currently gaining momentum, yet it remains challenging due to the lack of affordable methods to precisely isolate a single cell from a heterogeneous cell population without causing high cellular stress.
The QIAscout system is an effective and fast method to isolate viable single cells ensuring minimal manipulation of the cellular status. The QIAscout array is ideal for various cell types like adherent cells, suspension cells, cell lines, primary cells, and fluorescent cell lines providing an environment suitable for their growth and viability similar to any standard cell culture dish. The QIAscout array consists of 12,000 microrafts that can be selectively isolated containing the single cell of interest.
This novel single cell isolation method is ideal to separate single cells for further downstream analysis or cultivation of clonal sub-populations. Single cell isolation with QIAscout is compatible with multiple downstream applications such as whole genome and transcriptome amplification methods, PCR and NGS.