Webinars
Feb 20, 2017
1:00 PM–2:00 PM (EST)
Online
Webinar
Duration: 1hrs
Single cell analysis is getting into the focus of many research fields as it demonstrates the individual contribution of every cell in a heterogeneous population without obscuring a biological response that may otherwise occur when cells are assessed in bulk. Although single-cell research ins currently gaining momentum, yet it remains challenging due to the lack of affordable methods to precisely isolate a single cell from a heterogeneous cell population without causing high cellular stress.

The QIAscout system is an effective and fast methods to isolate viable single cells ensuring minimal manipulation of the cellular status. The QIAscout array is ideal for various cell types like adherent cells, suspension cells, cell lines, primary cells, and fluorescent cell lines providing an environment suitable for their growth and viability similar to any standard cell culture dish. The QIAscout array consists of 12,000 microrafts that can be selectively isolated containing the single cell of interest.

This novel single cell isolation methods is ideal to separate single cells for further downstream analysis or cultivation of clonal sub-populations. Single cell isolation with QIAscout is compatible with multiple downstream applications such as whole genome and transcriptome amplification methods, PCR and NGS.
Feb 21, 2017
9:30 AM–10:30 AM (EST)
Online
Webinar
Duration: 1hrs

Epigenetic changes, such as the methylation of cytosine, have been identified as key factors in various diseases. They play a crucial role in the regulation of important cellular processes, such as gene expression and cellular differentiation. Here, we describe a post-bisulfite library construction (PBLC) protocol that can overcome challenges frequently encountered with the classic whole genome bisulfite sequencing (WGBS) method, including low library yields and PCR amplification bias. We explore the establishment of a PBLC protocol that uses the combination of the EpiTect Fast Bisulfite Kit – for bisulfite conversion of non-methylated cytosines – along with the end-polishing and ligation chemistries of the QIAseq Ultralow Input Library Kit.

Feb 21, 2017
1:00 PM–2:00 PM (EST)
Online
Webinar
Duration: 1hrs

The gut microbiota composition changes dramatically throughout aging and disease. A healthy gut microbiota is typically characterized by large bacterial taxonomic diversity and functional capacity, whereas frailty and aging are associated with loss of diversity and expansion of more pathogenic bacterial species. However, in order to accurately profile changes in microbial communities, the reproducible isolation of high-quality DNA is an important step.  Automation enables reliable and reproducible isolation of DNA of superior quality, which can be used directly for downstream sequencing applications. In this webinar, we will discuss the development of a semi-automated workflow to profile the gut microbiota of young and old individuals and identify changes in bacterial composition and function that occur with age. This workflow will help to simplify and streamline the DNA extraction process for samples with high inhibitor content and subsequent microbial community analyses.

Feb 22, 2017
1:00 PM–2:00 PM (EST)
Online
Webinar
Duration: 1hrs
Pancreatic cancer is one of the most lethal malignancies with a poor prognosis. Understanding the mechanisms of tumor progression is therefore essential. Liquid biopsies are non-invasive methods for diagnostics to detect early stage cancer resulting in more successful treatment.  One liquid biopsy technique is the detection of RNA from tumor- derived exosomes. These exosomes participate in generating a metastatic niche. Using QIAGEN Bioinformatics solutions with a publicly available dataset, we have examined the transcriptome of Kupffer cells after uptake of pancreatic tumor-derived exosomes that induce the formation of a liver metastatic niche and analyzed the pathways and biological processes involved in this process.
Feb 23, 2017
1:00 PM–2:00 PM (EST)
Online
Webinar
Duration: 1hrs

Breast cancer is the most common cancer in women, accounting for 23% of all cancer diagnoses. Early detection of breast cancer metastasis would allow for changes in treatment strategy before the disease progression becomes apparent. In this webinar, we will demonstrate the enrichment and detection value – as well as the prognostic and predictive value – of the AdnaTestBreast in breast cancer.

 

Using the Combination of Combinations Principle (COCP), AdnaTest BreastCancer enables highly specific immunomagnetic cell selection for enriching circulating tumor cells (CTCs) from peripheral blood. It also allows sensitive analysis of breast cancer-associated gene expression in enriched CTCs by reverse transcription and PCR. The high performance of AdnaTest BreastCancer has been demonstrated in dozens of peer-reviewed publications. Several studies have reported that HER2 expression is different in primary tumors compared to CTCs. In addition, AdnaTestBreast HER2 overexpression profile corresponds to HER2 metastatic phenotype; and HER2 detected by the AdnaTestBreast and AdnaTest EMT-2/StemCell seems to initiate EMT and tumor stemness.

Feb 27, 2017
9:30 AM–10:30 AM (EST)
Online
Webinar
Duration: 1hrs

Breast cancer is the most common cancer in women, accounting for 23% of all cancer diagnoses. Early detection of breast cancer metastasis would allow for changes in treatment strategy before the disease progression becomes apparent. In this webinar, we will demonstrate the enrichment and detection value – as well as the prognostic and predictive value – of the AdnaTestBreast in breast cancer.

 

Using the Combination of Combinations Principle (COCP), AdnaTest BreastCancer enables highly specific immunomagnetic cell selection for enriching circulating tumor cells (CTCs) from peripheral blood. It also allows sensitive analysis of breast cancer-associated gene expression in enriched CTCs by reverse transcription and PCR. The high performance of AdnaTest BreastCancer has been demonstrated in dozens of peer-reviewed publications. Several studies have reported that HER2 expression is different in primary tumors compared to CTCs. In addition, AdnaTestBreast HER2 overexpression profile corresponds to HER2 metastatic phenotype; and HER2 detected by the AdnaTestBreast and AdnaTest EMT-2/StemCell seems to initiate EMT and tumor stemness.

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