PCR Master Mix Calculator
1. Choose PCR Product you want to work with.
Custom
Volume of template per reaction
µL
Reaction Volume
µL
Number of reactions
Extra reactions
Total volume
µL
Concentration
Volume
stock
Final
per max
for premix
PCR Buffer
x
x
Taq Polymerase
U/uL
U/uL
dNTP-Mix
mM
mM
Primer A
uM
uM
Primer B
uM
uM
Optional: MgCl2
mM
mM
Optional Tracer
x
x
Optional: Q-Solution
x
x
Water
Template DNA
Volume
per max
for premix
PCR Buffer
0 µL
0 µL
Taq Polymerase
0 µL
0 µL
dNTP-Mix
0 µL
0 µL
Primer A
0 µL
0 µL
Primer B
0 µL
0 µL
Optional: MgCl2
0 µL
0 µL
Optional Tracer
0 µL
0 µL
Optional: Q-Solution
0 µL
0 µL
Water
Template DNA
Volume without template
Volume with template
Related products
Cat. No. / ID. 203207
HotStarTaq DNA Polymerase (5000 U)
1 x 5000 units HotStarTaq DNA Polymerase, 1 x HotStarTaq Buffer Set (1 x 22 ml PCR Buffer, 1 x 40 ml Q-Solution, 1 x 22 ml MgCl2)
Cat. No. / ID. 203123 icon_0368_ls_gen_eco_friendly-s
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
Cat. No. / ID. 203144 icon_0368_ls_gen_eco_friendly-s
AllTaq Master Mix Kit (500)
For 500 x 20 µl PCR amplifications: 2 x 1.25 ml AllTaq Master Mix (4x), 1 x 200 µl Template Tracer (25x), 2 x 50 µl Master Mix Tracer (125x), 5 x 1.9 ml RNase-Free Water
Cat. No. / ID. RP725A
TaqNova DNA Polymerase (2500 U)
5 U/µL concentration with 10x TaqNova KCl Reaction Buffer, 10x TaqNova (NH4)2SO4 Reaction Buffer, and 50 mM MgCl2
Storage temperature: -20°C
Cat. No. / ID. RP905A
TaqNova HS DNA Polymerase (500 U)
TaqNova HS DNA Polymerase, 10x TaqNova HS Reaction Buffer, 50 mM MgCl2
Storage temperature: -20°C
Cat. No. / ID. 201203
Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
Cat. No. / ID. 201223
Taq PCR Core Kit (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2, dNTP Mix