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PCR Master Mix Calculator

1. Choose PCR Product you want to work with.
2. Fill in the volume of template DNA you want to use, the number of PCR reactions you intend to run and the individual reaction volume. 
You can add some extra volume for pipetting.
Volume of template per reaction
µL
Reaction Volume
µL
Number of reactions
Extra reactions
Total volume
µL
3. Fill in your stock concentrations and the desired final concentrations for components.
Concentration
Volume
stock
Final
per max
for premix
PCR Buffer
x
Taq Polymerase
U/uL
dNTP-Mix
mM
Primer A
uM
Primer B
uM
Optional: MgCl2
mM
Optional Tracer
x
Optional: Q-Solution
x
Water
Template DNA
Volume
per max
for premix
PCR Buffer
0 µL
0 µL
Taq Polymerase
0 µL
0 µL
dNTP-Mix
0 µL
0 µL
Primer A
0 µL
0 µL
Primer B
0 µL
0 µL
Optional: MgCl2
0 µL
0 µL
Optional Tracer
0 µL
0 µL
Optional: Q-Solution
0 µL
0 µL
Water
Template DNA
Volume without template
Volume with template
 

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