TaqNova HS DNA Polymerase

For high PCR-specificity applications with fast enzyme activation time

S_1356_1_LS_OEM_TaqNova_HS_DNA_Polymerase_200_U
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TaqNova HS DNA Polymerase (200 U)

Cat. No. / ID:  RP902A

TaqNova HS DNA Polymerase, 10x TaqNova HS Reaction Buffer, 50 mM MgCl2 Storage temperature: -20°C
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$104.00
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Quantity
200 U
500 U
2500 U
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The TaqNova HS DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM

Features

  • Catalyzes DNA synthesis in a 5’ → 3’ direction
  • Shows no 3′ → 5’ exonuclease activity but has a 5’ → 3’ exonuclease activity
  • Enables easy setup of a hot-start PCR reaction at room temperature
  • Minimizes amplification of non-specific products and primer-dimers
  • Amplifies fragments of up to 5 kb and leaves ´A´ overhangs

Product Details

TaqNova HS DNA Polymerase is a mixture of of recombinant thermostable Taq DNA polymerase from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy setup of a hot-start PCR reaction at room temperature.


It is recommended for a wide range of demanding applications that require highly specific amplification. The TaqNovaHS polymerase is a universal and easy-to-use DNA polymerase that works rapidly and effectively in various PCR conditions. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3′ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.


It is supplied with 20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.


One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.

 

Performance

Assay Specification
Functional activity Passed
DNase contamination None detected
Single-stranded exonuclease None detected
Double-stranded exonuclease None detected
Double-stranded endonuclease None detected

 

Principle

The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures, which prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The antibody is released from the polymerase during normal cycling conditions. The use of the TaqNovaHS Polymerase does not require any additional incubation step to activate the enzyme.

Procedure

Quality Control

DNase activity and DNase contamination were evaluated by incubating 1 μg of DNA with 10 units of the enzyme for 4 hours at 37°C. Results are visualized on an ethidium bromide-stained agarose gel. The product was extensively tested in PCR reaction.

Applications

This is used for applications such as:

  • Hot-start and real-time PCR
  • Singleplex and multiplex PCR
  • PCR with DNA from various kinds of specimen
  • Specific amplification of difficult templates (GC-rich)

 

Resources

Quick-Start Protocols (1)
Safety Data Sheets (1)
Certificates of Analysis (1)