qPCR Gene Expression
Gene expression is based on the quantification of corresponding mRNA or lncRNA, which is crucial for various fields, including cancer and disease research and biomarker identification. For the quantification of known targets, reverse transcription qPCR (RT-qPCR) remains the gold standard method, providing fast analysis of just a few or many genes.
mRNA expression profiling involves measuring the levels of messenger RNA (mRNA) to understand gene expression patterns, while miRNA profiling focuses on the quantification of microRNAs (miRNAs), which regulate gene expression. Both techniques are essential for gaining knowledge of cellular processes and disease mechanisms. Our gene expression products enable sensitive quantification of any type of RNA or transcript abundance, delivering unbiased gene expression profiles.
qPCR Chemistry
Process controls and features increase your success
Ultrafast one- or two-step RT-PCR procedure
Which QuantiNova kit is right for your workflow?
Complete Assay Systems
What you need to get started
Both systems use a universal reverse transcription kit (1). Each system has its own dedicated PCR mastermix (2), which is combined with your choice of predesigned or custom assays or panels (3).
QuantiNova LNA PCR System |
QuantiNova LNA Probe PCR System |
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Detection technology | SYBR® Green | FAM-labeled, LNA-enhanced hydrolysis probe |
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1 | Reverse Transcription | QuantiNova Reverse Transcription Kit | QuantiNova Reverse Transcription Kit |
2 | Mastermix for qPCR | QuantiNova SYBR Green PCR Kit | QuantiNova Probe PCR Kit |
3 | Assay or panel | • QuantiNova LNA PCR Assay • QuantiNova LNA PCR Custom Assay • QuantiNova LNA PCR Focus Panels • QuantiNova LNA PCR lncRNA Focus Panels • QuantiNova LNA PCR Flexible Panels • QuantiNova LNA PCR Custom Panels |
• QuantiNova LNA Probe PCR Assay • QuantiNova LNA Probe PCR Custom Assay • QuantiNova LNA Probe PCR Focus Panels • QuantiNova LNA Probe PCR lncRNA Focus Panels • QuantiNova LNA Probe PCR Flexible Panels • QuantiNova LNA Probe PCR Custom Panels |
The top benefits of QuantiNova LNA PCR
QuantiNova LNA PCR by the numbers
Fast workflow for all sample types, including tissues, cells, exosomes and FFPE samples
Products
Real-Time Cyclers
Raise your qPCR workflow to the next level
It is increasingly challenging to find the right real-time PCR cycler for your laboratory’s application requirements. For best results, you need low operating costs, high throughput, the ability to multiplex with fast analysis times and reliable reproducibility. As the list of features and specifications grows, an understanding of some of the key performance benefits such as precision, uniformity, dynamic range and resolution becomes critical.
Choose from our portfolio of real-time PCR cyclers the instrument that suits your needs