Real-time qPCR, also known as real-time quantitative polymerase chain reaction, is a powerful technique for amplifying and quantifying targeted DNA molecules. This method combines PCR amplification with fluorescent dye detection, allowing for the monitoring of the reaction in real time. Key features of real-time qPCR include high sensitivity, specificity and the ability to provide quantitative data on gene expression levels, making it an indispensable tool in various research and diagnostic applications.
Our real-time qPCR solutions are designed to enhance your workflow with unparalleled accuracy and efficiency. Whether you are conducting gene expression analysis, genotyping or pathogen detection, our advanced platforms offer reliable performance and reproducible results. Explore our comprehensive range of real-time qPCR products to elevate your research and achieve precise, high-quality data. Trust in our expertise to support your real-time polymerase chain reaction needs and drive your scientific discoveries forward.
Applications
qPCR Chemistry
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Looking for one of our other qPCR solutions?
Does your project have unique requirements? Our complete range of real-time PCR solutions has you covered. Check out these other offerings or contact one of our experts to find the best tools for your research.
Experiment Design
Ensuring high-quality, reproducible results
qPCR is a ubiquitous research tool, but improperly designed experiments will generate questionable findings. The MIQE guidelines define the standards for designing and reporting qPCR experiments.
What makes a good qPCR assay? High accuracy, dynamic range, reliability, reproducibility, sensitivity and specificity. The primer design algorithm and the reaction buffer conditions must work together to provide the best results. Designing assays without fully understanding the critical design aspects can unknowingly compromise the results.
We can help remove the guesswork from your experiments. Our broad selection of qPCR kits and an unparalleled choice of predesigned assays and panels along the option to design your own using our advanced algorithms ensure quality qPCR results time and again.
Consider these factors for useful and meaningful results
Resources and Tools
Latest – webinars and other tools
Guides and tips for great results
Real-Time Cyclers
Raise your qPCR workflow to the next level
It is increasingly challenging to find the right real-time PCR cycler for your laboratory’s application requirements. For best results, you need low operating costs, high throughput, the ability to multiplex with fast analysis times and reliable reproducibility. As the list of features and specifications grows, an understanding of some of the key performance benefits such as precision, uniformity, dynamic range and resolution becomes critical.
Choose the instrument that suits your needs from our portfolio of real-time PCR cyclers.