What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?

Expected PCR results when using the EpiTect Control DNA Set in combination with the EpiTect MSP Kit can be found in the table below:

 

Type of DNA Primer for unmethylated & unconverted genomic DNA Primer for unmethylated & bisulfite converted target DNA Primer for methylated & bisulfite converted target DNA
Unmethylated & untreated control DNA PCR product NO PCR product NO PCR product
Unmethylated & bisulfite converted control DNA NO PCR product PCR product NO PCR product
Methylated & bisulfite converted control DNA NO PCR product NO PCR product PCR product
No template control NO PCR product NO PCR product NO PCR product

 

 

 

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