FAQ-3985
What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?
The yield and integrity of genomic DNA depend on the disruption and homogenization method used with the QIAwave DNA/RNA kits.
Homogenization with the TissueRuptor II (or other rotor-stator homogenizer) or the TissueLyser III results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, resulting in higher DNA yields.
Homogenization with the TissueRuptor II (or other rotor-stator homogenizer) or the TissueLyser III results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, resulting in higher DNA yields.
Related products
QIAwave DNA/RNA Mini Kit
The QIAwave DNA/RNA Mini Kit is a more eco-friendly version of our standard AllPrep DNA/RNA Mini Kit designed for purifying both genomic DNA and total RNA from the same cell or tissue sample. Since there is no need to divide the sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application.
The QIAwave DNA/RNA Mini Kit uses 52% less plastic and 58% less cardboard than our standard kit and offers Waste Tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the box lid. So, while the kit packaging and components of our QIAwave DNA/RNA Mini Kit may look different, it’s as easy to use as the AllPrep DNA/RNA Mini Kit, and the chemistry and performance are identical.
Please be aware that you will need sterile glass bottles to store the reconstituted buffers.
In partnership with My Green Lab, we've assessed the environmental impact of this kit. My Green Lab ACT labels evaluate and score products on several sustainability criteria:
Manufacturing
Responsible chemical management
Sustainable content within products and packaging materials
Disposal of the packaging at the end of life
Products are scored from 1 to 10 except for energy and water consumption, which are scored 1 point per kWh or gallon, respectively. A low score means a lower impact (see figures "QIAwave DNA/RNA Mini Kit ACT label US, EU and UK").