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The integrity of my DNA sample (e.g. QIAxcel Connect) is bad.
Make sure to use the ‘Fast’ version of the EZ2 AllPrep DNA/RNA FFPE protocol as this provides less fragmented DNA. However, this can negatively impact its performance in other downstream applications such as qPCR.
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EZ2 AllPrep DNA/RNA FFPE Kit
The EZ2 AllPrep DNA/RNA FFPE Kit is used with the EZ2 Connect instrument. It automates the extraction of both DNA and RNA, from exactly the same piece of sample in two distinct elution fractions.
The kit technology allows the reversal of crosslinking caused by the sample’s exposure to formalin without overnight proteinase K incubation.