Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?
Yes, it is possible. Mix 20 µl of Cignal lentiviral particles with SureENTRY Transduction Reagent into 30 µl growth medium (without antibiotics) and then add the mixture to the cells. The final concentration of SureENTRY Transduction Reagent is 8 µg/ml in each well.