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Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?
No. The IC RNA Assay is detected in Cy5. The SARS-CoV-2 Neo Assay detects two viral targets in the Cy5 channel. When using the IC RNA with the SARS-CoV-2 Neo Assay, the signals cannot be discriminated.
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SARS-CoV-2 Neo Assay Kit
To ensure future-proof detection of SARS-CoV-2, we developed a novel assay kit covering six targets across four genomic regions (ORF1ab, ORF3a, ORF7a and N gene) of the virus. Using multiple targets in multiple regions increases the likelihood of successful detection.
Probes are coupled to different dyes and can be measured in the FAM, ROX and Cy5 channels on a cycler of your choice.
This kit can be used in combination with the QIAprep& Viral RNA UM Kit chemistry and SARS-CoV-2 Neo Positive Control to provide fast and easy detection. Sample types compatible for detecting SARS-CoV-2 include nasal, nasopharyngeal, oropharyngeal swabs, lolly (lollipop swab), saliva, and gargles.
QIAprep& Viral RNA UM Kit
The QIAprep& Viral RNA UM Kit is an innovative liquid-based approach to viral RNA epidemiology. The kit is optimized for ultrafast sample preparation and sensitive real-time PCR detection of enveloped RNA viruses such as coronaviruses from human samples (nasal, oro- and nasopharyngeal swabs) collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. The kit is also compatible with neat saliva and gargle samples. Fewer and simpler workflow steps accelerate the turnaround time and increase testing frequency while decreasing plastic usage, cost and hands-on time. The method can be seamlessly integrated into automated workflows with standard lab equipment at all throughput needs. The QIAprep& Viral RNA QIAsymphony Kit can be used for automated sample preparation and assay set up on the QIAsymphony SP/AS. The QIAprep& Viral RNA QIAsymphony Kit requires the installation of a custom protocol available from QIAGEN Services.
The QIAprep& Viral RNA UM Kit can be used in conjunction with the SARS-CoV-2 N1+N2 Assay Kit.
Want to try the QIAprep& Viral RNA UM Kit for the first time? Register to start your trial.
For labs that need to track currently relevant mutations of the virus, wet-lab tested LNA-based qPCR genotyping assays have been designed to detect the variants of concern (VOCs). The assay performance in terms of specificity and sensitivity has been verified using the QIAprep& Viral RNA UM Kit. View the assays here.