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How long can I store the reconstituted Internal Control and the reconstituted Positive Control?
Reconstituted QIAcuity Mycoplasma Internal Control aliquots can be stored at −30°C to −15°C for up to six weeks. More than five freeze–thaw cycles should be avoided.
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QIAcuity Mycoplasma Quant Kit and Standards
The QIAcuity Mycoplasma Quant Kit reliably detects and quantifies the presence of mycoplasma rRNA in various sample types. Combined with a recommended sample prep method, we offer a NAT (Nucleic Acid Technique) workflow for mycoplasma testing that is compliant with the European, US and Japanese Pharmacopeia. The robust process eliminates time-consuming cultivation of mycoplasma. The workflow is particularly suited to sample types of varying purity (for example, cell bank samples, in-process samples, such as virus harvest and final lots/batches).
The QIAcuity Mycoplasma Quant Kit provides a consistent, accurate, precise and highly reproducible detection of mycoplasma presence. The detection via rRNA enables a higher sensitivity than using DNA because of the multiple copies present in a single bacterial cell. The assay is still able to detect DNA if the RT-step is skipped, enabling a high degree of flexibility.
The kit is compatible with the QIAcuity Digital PCR System and the QIAcuity Nanoplates.
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