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What is the recommended HpaII concentration?
Enzyme concentration depends on sample input and incubation time. When following our protocol described in the handbook HpaII enzymes work in the following range:
- IVGN0936: 0.025 – 0.125 U/µL
- ER0511/ER0512: 0.25 – 0.5 U/µL
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Viral Vector Direct Lysis Kits
Adeno-associated virus and lentivirus are widely used viral vectors in cell and gene therapy applications. However, the process of generation and purification of the viral vectors requires precise quality control to enable safe and reliable dosing during clinical studies or patient care. The ability to accurately quantify vector titers as well as determine contaminations is key to safe and effective AAV-based and LV-based therapies.
With the introduction of direct vector lysis solutions for AAV or LV that include reagents for either 100 or 1000 reactions, we now have an optimized and standardized workflow for direct viral vector lysis through to an accurate and precise way of determining the viral titer.