Product FAQ - QIAGEN



Product FAQ Print Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control? FAQ ID - 3724 View Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit? FAQ ID - 3725 View Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately? FAQ ID - 3726 View Does QIAGEN offer an anti-GFP gapmer control? FAQ ID - 3739 View Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1? FAQ ID - 3745 View How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C? FAQ ID - 3744 View Do I need to buy special kits for the QIAcube Connect? FAQ ID - 141485 View Can I program my own protocols for the QIAcube Connect? FAQ ID - 141486 View Can the QIAcube Connect rotor and/or buckets be removed for cleaning? FAQ ID - 141491 View Can purification columns from other suppliers be processed on the QIAcube Connect? FAQ ID - 141487 View How can I get software updates for the QIAcube Connect? FAQ ID - 141494 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect? FAQ ID - 141496 View Do I need to discard partially used QIAcube Connect tip racks? FAQ ID - 141499 View How can I decontaminate the QIAcube Connect system? FAQ ID - 141500 View Can the QIAcube Connect heater/shaker be used independently from protocol runs? FAQ ID - 141502 View How can I load new protocols onto the QIAcube Connect? FAQ ID - 141501 View What dedicated QIAcube Connect kits are available? FAQ ID - 141504 View Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect? FAQ ID - 1414507 View Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings? FAQ ID - 141505 View Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect? FAQ ID - 141506 View Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? FAQ ID - 141508 View How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect? FAQ ID - 141509 View Can the CB and RB sample tubes be used interchangeably? FAQ ID - 141510 View Which network settings are supported by the QIAcube Connect and Q-Base? FAQ ID - 141517 View Why can I not find the Q-Base device in the device list during the first installation? FAQ ID - 141516 View Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App? FAQ ID - 1414518 View Why is the QIAcube Connect App reacting so slowly? FAQ ID - 141519 View Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)? FAQ ID - 1414520 View Why does the connection test fail? FAQ ID - 141522 View How critical is the time of the temperature change from 60°C to 80°C? FAQ ID - 143768 View Is it possible to use Lyse&Spin baskets? FAQ ID - 143762 View Why is DTT used only for semen samples? FAQ ID - 143761 View Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells? FAQ ID - 143079 View Can the lysis volume be reduced for higher sensitivity? FAQ ID - 143763 View What if only one heater shaker is available? FAQ ID - 143764 View Why is there no male DNA obtained from sexual assault samples? FAQ ID - 143767 View If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.) FAQ ID - 143766 View Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable? FAQ ID -147395 View What RNAs has QIAseq FastSelect been designed to remove? FAQ ID -147910 View How does QIAseq FastSelect work? FAQ ID -147907 View Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature? FAQ ID -147909 View Will QIAseq FastSelect work in other species? FAQ ID -147911 View What library prep kits has QIAseq FastSelect been tested with? FAQ ID -147912 View I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit? FAQ ID -147913 View What RNA range has the QIAseq FastSelect been tested with? FAQ ID -147914 View Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S? FAQ ID -147915 View Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.? FAQ ID -147916 View Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription? FAQ ID -1 View How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32 View When is chloramphenicol amplification of plasmids performed? FAQ ID -3 View What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit? FAQ ID -45 View What are the most commonly used protease inhibitors? FAQ ID -53 View How should I propagate pQE expression plasmids? FAQ ID -58 View How can I increase the amount of soluble recombinant protein in E. coli expression? FAQ ID -64 View How can I remove imidazole from a protein sample? FAQ ID -91 View Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97 View How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28 View How should I store plant material for DNA isolation using the DNeasy Plant Kit? FAQ ID -114 View Do I need to use an RNase inhibitor in my RT reaction? FAQ ID -119 View Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? FAQ ID -127 View Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup? FAQ ID -130 View How does imidazole affect my quantitation of protein? FAQ ID -132 View Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel? FAQ ID -133 View What is the difference between disruption and homogenization in the RNeasy System? FAQ ID -139 View What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips? FAQ ID -142 View Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins? FAQ ID -147 View What is the genotype of the EZ Competent Cells? FAQ ID -157 View What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit? FAQ ID -160 View What are the molecular weights of proteins in the 6xHis Protein Ladder? FAQ ID -169 View How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent? FAQ ID -176 View How can I improve recoveries when using the QIAquick Kits? FAQ ID -180 View How can I improve transfection efficiency using Effectene Transfection Reagent? FAQ ID -181 View What is the principle behind Effectene Transfection Reagent? FAQ ID -184 View What is the composition of Buffer EB? FAQ ID -199 View Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps? FAQ ID - 3709 View Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C? FAQ ID - 3711 View What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits? FAQ ID - 3715 View Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)? FAQ ID - 3716 View How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023 View What is the recipe for 1x PBS solution? FAQ ID -1030 View Where can I find a protocol for cleanup of already purified plasmid DNA? FAQ ID -1031 View How do I perform an Acetone Precipitation for concentrating and desalting protein samples? FAQ ID -1035 View What is the difference between Buffers RLT and RLT Plus? FAQ ID -1043 View What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit? FAQ ID -1047 View Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel? FAQ ID -1051 View Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase? FAQ ID -1052 View How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits? FAQ ID -1061 View What should I use as a standard for absolute quantification in real-time PCR? FAQ ID -1085 View What does transcript variant mean when searching for specific QuantiTect Primer Assays? FAQ ID -1137 View What is a QuantiTect Primer Assay? FAQ ID -1141 View Do QuantiTect Primer Assays contain SYBR Green dye? FAQ ID -1143 View What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II? FAQ ID -1218 View What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit? FAQ ID -1220 View What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II? FAQ ID -1221 View Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA? FAQ ID -1250 View Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA? FAQ ID -1251 View What is the pH value of QIAGEN's Nuclease-Free Water? FAQ ID -1290 View Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 View Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA? FAQ ID -1328 View Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time? FAQ ID -1352 View How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit? FAQ ID -1354 View What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit? FAQ ID -1356 View How do I resuspend my FlexiPlate siRNAs? FAQ ID -1360 View Are there any specific cleaning solutions recommended for the QIAcube? FAQ ID -1416 View Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 View Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1577 View What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1580 View What is the binding capacity of the Ni-NTA Superflow Cartridges? FAQ ID -1603 View How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644 View Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer? FAQ ID -1659 View Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs? FAQ ID -1664 View What is the composition of Buffer PE? FAQ ID -1691 View What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738 View How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced? FAQ ID -1838 View Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer? FAQ ID -1848 View What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID -1852 View If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated? FAQ ID -1858 View What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901 View Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913 View How much sample can be used with the RNeasy Plus 96 Kit? FAQ ID -1992 View What is the composition of Buffer P2? FAQ ID -203 View A white precipitate has formed in my 10x RT buffer. Is it still ok to use? FAQ ID -216 View For how long are BioMag Particles stable? FAQ ID -262 View I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing? FAQ ID -263 View Can I clean up my DNase treated RNA samples using RNeasy columns? FAQ ID -286 View How can one determine the optimal annealing temperature for a specific PCR assay? FAQ ID -288 View What is the composition of the QIAGEN Multiplex PCR Buffer? FAQ ID -289 View Can I use HEPES buffer instead of phosphate in my Ni-NTA column? FAQ ID -291 View Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit? FAQ ID -293 View What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit? FAQ ID -2004 View What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029 View How can a C-terminal 6xHis-tagged QIAgene be expressed? FAQ ID -2041 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results? FAQ ID -2079 View Does the Rotor-Gene Q software have the capacity to export data? FAQ ID -2089 View Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 View Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163 View Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types? FAQ ID -2190 View How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 View What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2203 View What buffer conditions give the best resolution for agarose gel electrophoresis? FAQ ID -2256 View How much DNA should be loaded per well of an agarose gel? FAQ ID -2259 View Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 View What is the "seed sequence" of a miRNA? FAQ ID -2262 View Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits? FAQ ID -2301 View Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit? FAQ ID -2315 View How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit? FAQ ID -2340 View How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? FAQ ID -2341 View How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? FAQ ID -2350 View Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? FAQ ID -2371 View Can I buy QIAquick and MinElute columns separately? FAQ ID -2460 View What is the PAXgene 96 filter plate? FAQ ID -2486 View Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube? FAQ ID -2509 View Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System? FAQ ID -2514 View How long is the fixation time? FAQ ID -2519 View What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits? FAQ ID -2543 View Are there any accessories required for sample disruption with the TissueLyser LT? FAQ ID -2551 View Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit? FAQ ID -2603 View During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit FAQ ID -2607 View What is the SBTengine® software? FAQ ID -2628 View How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment? FAQ ID -2654 View What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis? FAQ ID -2659 View How can I avoid or remove genomic DNA contamination from the total RNA preparation? FAQ ID -2662 View What are the differences between one-step and two-step RT--PCR? FAQ ID -2666 View What is qPCR? FAQ ID -2668 View What are the common primer and probe chemistries utilized for qPCR assays? FAQ ID -2669 View What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes? FAQ ID -2671 View What negative controls are typically included in qPCR and/or qRT-PCR experiments? FAQ ID -2672 View What positive controls are typically included in qPCR and/or qRT-PCR experiments? FAQ ID -2673 View What are the guidelines for choosing a housekeeping gene for normalizing qPCR results? FAQ ID -2674 View Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays? FAQ ID -2675 View How does HotStart PCR help minimize nonspecific amplification events? FAQ ID -2676 View What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry? FAQ ID -2678 View What is the delta Rn value? FAQ ID -2681 View What is the threshold cycle or Ct value? FAQ ID -2682 View Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay? FAQ ID -2684 View Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay? FAQ ID -2685 View Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay? FAQ ID -2686 View Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase? FAQ ID -2689 View Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC? FAQ ID -2690 View What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed? FAQ ID -2691 View What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach? FAQ ID -2692 View What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed? FAQ ID -2693 View How do I determine the amplification efficiency of my qPCR assay? FAQ ID -2694 View How do I determine the linear dynamic range of my qPCR or qRT-PCR assay? FAQ ID -2696 View What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them? FAQ ID -2705 View What do I need to complete a RT² qPCR Primer Assay? FAQ ID -2707 View How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 View What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 View How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 View How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 View At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 View What is RNA interference (RNAi)? FAQ ID -2755 View What are the key factors for success in an RNAi experiment? FAQ ID -2758 View What criteria should one use in choosing between siRNA versus shRNA for their studies? FAQ ID -2771 View How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 View What are the SureSilencing shRNA Plasmids? FAQ ID -2784 View What is the composition of Buffer S3? FAQ ID -2788 View What is the composition of Buffer ETR? FAQ ID -2789 View What is the composition of Buffer BB? FAQ ID -2790 View What is the composition of Buffer RLT plus? FAQ ID -2794 View What is the composition of Buffer RLC? FAQ ID -2795 View What is the composition of Buffer RPE? FAQ ID -2797 View What is the composition of Buffer RWT? FAQ ID -2798 View What is the composition of gDNA Eliminator Solution? FAQ ID -2799 View What is the composition of Buffer RDD? FAQ ID -2800 View What is the composition of Buffer PKD? FAQ ID -2801 View What is the composition of Buffer ALO? FAQ ID -2805 View What is the composition of RBC Lysis Solution? FAQ ID -2808 View What is the composition of Cell Lysis Solution? FAQ ID -2809 View What is the composition of Protein Precipitation Solution? FAQ ID -2810 View What is the composition of Cell Suspension Solution? FAQ ID -2811 View What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits? FAQ ID -2812 View What is the composition of DNA Hydration Solution in Gentra Puregene Kits? FAQ ID -2813 View What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841 View What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0? FAQ ID -2849 View Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest? FAQ ID -2894 View Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -2926 View What is the composition of Buffer G2? FAQ ID -2943 View What is the composition of Buffer B1? FAQ ID -2944 View What is the composition of Buffer B2? FAQ ID -2945 View How much DNA does a human cell contain? FAQ ID -2948 View How much RNA does a bacterial cell contain? FAQ ID -2949 View How much DNA and RNA can be expected from human blood cells? FAQ ID -2950 View What is the cellular composition of human blood? FAQ ID -2951 View How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation? FAQ ID -2954 View Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers? FAQ ID -2980 View Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2987 View What is the sequence of the miScript Universal Primer? FAQ ID -2994 View How can I keep my centrifuge tubes endotoxin-free? FAQ ID -301 View Can Buffers N3 and P3 be used interchangeably? FAQ ID -310 View Are QIAprep and QIAquick Spin columns interchangeable? FAQ ID -311 View Can I store agarose gel slices containing DNA for gel extraction at a later point? FAQ ID -313 View How can QIAGEN Protease and Proteinase K be inactivated? FAQ ID -315 View Does the 5x OneStep RT-PCR Buffer contain BSA? FAQ ID -326 View What is the origin of replication and the plasmid copy number of the pQE vectors? FAQ ID -338 View Is QIAGEN carrier RNA sold separately? FAQ ID -351 View What is the white insoluble precipitate in my resuspended plasmid DNA pellet? FAQ ID -352 View What is the composition of PBS? FAQ ID -361 View How can I express toxic protein in E. coli? FAQ ID -373 View How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? FAQ ID -374 View What are the dissociation constants for the Anti-His Antibodies? FAQ ID -385 View What is the average molecular weight of a siRNA, and how do I convert uM to ug values? FAQ ID -388 View Can siRNA silence bacterial genes? FAQ ID -395 View How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit? FAQ ID -3003 View Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples? FAQ ID -3032 View Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits? FAQ ID -3053 View Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings? FAQ ID -3054 View What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced? FAQ ID -3055 View What is the thermal output for a BioRobot MDx and BioRobot Universal? FAQ ID -3056 View What is the thermal output for a QIAsymphony SP and AS instrument? FAQ ID -3057 View How precise is the QIAgility? FAQ ID -3063 View What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility? FAQ ID -3064 View How long does it take to set up a 96-well plate PCR reaction on the QIAgility? FAQ ID -3066 View Can I use a bar code scanner or reader on the QIAgility? FAQ ID -3067 View How can I find the run parameters for a BioSprint protocol that I am running? FAQ ID -3069 View Can BioSprint 96 be connected to LIMS? FAQ ID -3070 View What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit? FAQ ID -3081 View What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits? FAQ ID -3082 View What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols? FAQ ID -3083 View What is the concentration of the different Proteinase K solutions sold by QIAGEN? FAQ ID -3084 View Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit? FAQ ID -3085 View What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)? FAQ ID -3086 View Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit? FAQ ID -3088 View What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit? FAQ ID -3096 View How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 View Can we do modification XXXXX for custom siRNA that is not on the web page? FAQ ID -3107 View What is the stability of siRNA lyophilized and resuspended? FAQ ID -3109 View Do you offer FlexiTube or FlexiPlate siRNAs for rat? FAQ ID -3114 View Is it possible to extract RNA from frozen blood? FAQ ID -3119 View Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 View What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails -48 View Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit? FAQ ID -3125 View Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor? FAQ ID -3132 View How do I remove the RNA contamination from purified DNA? FAQ ID -3133 View What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini? FAQ ID -3140 View What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ? FAQ ID -3169 View What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs? FAQ ID -3174 View What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed? FAQ ID -3175 View What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q? FAQ ID -3178 View Is REST software Windows 7 compatible? FAQ ID -3182 View Can the Rotor-Gene Q perform gradient PCR? FAQ ID -3183 View What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 View What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 View What is LLOQ? FAQ ID — 3401 View What is the difference between LLOQ and LOD? FAQ ID — 3402 View What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays? FAQ ID - 3427 View What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit? FAQ ID - 3429 View Can the miScript II RT Kit be used to quantify plant miRNAs? FAQ ID - 3431 View What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system? FAQ ID - 3444 View Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation? FAQ ID - 3467 View What is the proper disposal procedure for waste from the sample preparation? FAQ ID - 3470 View What is the expected yield from 8.5 ml of blood? FAQ ID - 3496 View What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain? FAQ ID - 3501 View How should blood samples drawn into PAXgene Blood DNA Tubes be transported? FAQ ID - 3507 View How long can samples in PAXgene Blood DNA Tubes be stored at room temperature? FAQ ID - 3508 View What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method? FAQ ID - 3510 View Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3512 View What does PAXgene Tissue STABILIZER contain? FAQ ID - 3513 View What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield? FAQ ID - 3516 View Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing? FAQ ID - 3517 View What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit? FAQ ID - 3519 View Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material? FAQ ID - 3521 View Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library? FAQ ID - 3534 View Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used? FAQ ID - 3559 View Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3573 View Is clot detection possible on the QIAsymphony? FAQ - ID 3588 View How can I determine if a tube is suitable for clot detection on QIAsymphony? FAQ ID - 3590 View How should I thaw frozen plasma samples? FAQ ID - 3645 View How do Unique Molecular Indexes (UMIs) improve quantification? FAQ ID - 3669 View Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)? FAQ ID - 3686 View I would like to stop during the library preparation procedure. When can I do that? FAQ ID - 3687 View Which regions are covered by the GR DNAseq V2 Panels (#181900)? FAQ ID - 3688 View When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off? FAQ ID - 3690 View How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber? FAQ ID - 3691 View Are peak heights of Q48 comparable to other PyroMark instruments? FAQ ID - 3692 View How much space does the PyroMark Q48 Autoprep instrument need? FAQ ID - 3693 View Can unused wells in a pyrosequencing discs be used in the next run? FAQ ID - 3694 View Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)? FAQ ID - 3695 View What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)? FAQ ID - 3696 View What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center? FAQ ID - 3698 View How do I prepare Buffer MP? FAQ ID - 3620 View Are the QIAshredder columns included in various QIAGEN kits the same? FAQ ID - 3624 View What is the composition of buffer QBT? FAQ ID -411 View What is the composition of buffer QC? FAQ ID -412 View What is the composition of buffer QF? FAQ ID -413 View What is the composition of buffer STE? FAQ ID -415 View What is the composition of buffer TE? FAQ ID -416 View What is the composition of buffer FWB2? FAQ ID -417 View What is the composition of buffer P3? FAQ ID -418 View What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors? FAQ ID -470 View Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 View Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed? FAQ ID -474 View Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit? FAQ ID -482 View Can Ni-NTA resins be used to purify protein with an internal His-tag? FAQ ID -496 View How do I calculate the percentage of silencing with real-time RT-PCR for siRNA? FAQ ID -498 View Do you have a protocol for the removal of endotoxins from already purified plasmid DNA? FAQ ID -500 View Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit? FAQ ID -518 View What is the composition of the siRNA resuspension & annealing buffer? FAQ ID -522 View Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls? FAQ ID -523 View What is Tissue-Tek O.C.T., and what is it used for? FAQ ID -530 View Why do replicates in real-time PCR have different plateau heights? FAQ ID -539 View How can I avoid primer-dimer formation during PCR amplification? FAQ ID -544 View How can I tell if I have primer-dimers in my PCR reaction? FAQ ID -552 View How should I store cDNA produced using Omniscript Reverse Transcriptase? FAQ ID -561 View Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits? FAQ ID -563 View What is the composition of Buffer ER? Is it available separately? FAQ ID -571 View What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried? FAQ ID -572 View Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit? FAQ ID -575 View Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? FAQ ID -577 View What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit? FAQ ID -600 View Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit? FAQ ID -603 View What do you recommend for the cleanup of genomic DNA (gDNA)? FAQ ID -618 View How can I improve my RNA yield from liver sample when using RNeasy Kits? FAQ ID -623 View Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100 View What 96-well plates do you recommend for use with the LiquiChip Workstation? FAQ ID -630 View What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample? FAQ ID -631 View Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? FAQ ID -633 View Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing? FAQ ID -637 View What is the shelf-life of my QIAGEN Kit? FAQ ID -651 View What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification? FAQ ID -665 View Can I purchase Phi29 DNA polymerase only? FAQ ID -708 View What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit? FAQ ID -721 View I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20 View I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745 View How much DNA is obtained in the average PCR reaction? FAQ ID -750 View Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA? FAQ ID -754 View Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns? FAQ ID -759 View How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN? FAQ ID -762 View What is the difference between Ni-NTA Agarose and Ni-NTA Superflow? FAQ ID -764 View What is the composition of Buffer N3? FAQ ID -767 View Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa? FAQ ID -770 View How should fluorescent labeled probes be stored? FAQ ID -784 View Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA? FAQ ID -785 View Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable? FAQ ID -786 View How can I get specific information and a quote for my individual Sequencing Service needs? FAQ ID -792 View Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells? FAQ ID -795 View How can I separate PCR fragments that are small and very close in size on an agarose gel? FAQ ID -800 View Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins? FAQ ID -802 View What Disinfectant Solution do you recommend for the BioRobot MDx? FAQ ID -809 View Is the E. coli Host Strain M15[pREP4] resistant to Zeocin? FAQ ID -842 View What annealing temperature should be used with the QuantiTect Primer Assays? FAQ ID -849 View Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 View Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent? FAQ ID -865 View Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis? FAQ ID -879 View Do you have a protocol for the isolation of genomic DNA from frozen clotted blood? FAQ ID -900. Test View Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)? FAQ ID -907 View Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908 View What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy? FAQ ID -942 View Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA? FAQ ID -959 View Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates? FAQ ID -971 View Do you have a protocol for the purification of PCR products using the BioSprint System? FAQ ID -984 View Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent? FAQ ID -997 View How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE? FAQ ID - 3707 View How can I increase expression of my 6xHis-tagged protein in E. coli? FAQ ID -63 View Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit? 148723 View What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit? 148724 View What is the composition of buffer OW2? FAQ ID -419 View What is the composition of buffer OBB? FAQ ID -421 View Do I need to buy special kits for the QIAcube Connect MDx? 148725 View What applications are offered on the QIAcube Connect MDx instrument? 148726 View Can I program my own protocols for the QIAcube Connect MDx? 148727 View Can purification columns from other suppliers be processed on the QIAcube Connect MDx? 148728 View What are the dimensions and weight of the QIAcube Connect MDx? 148729 View Can the QIAcube rotor adapters be autoclaved? 148730 View Can the QIAcube rotor and/or buckets be removed for cleaning? 148731 View Can the QIAcube be connected to a laboratory information management system? 148732 View Are run reports and/or log files available on the QIAcube? 148733 View How can I get software updates for the QIAcube Connect MDx? 148734 View Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion? 148735 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? 148736 View Do I need to discard partially used QIAcube tip racks? 148738 View How can I decontaminate the QIAcube Connect MDx system? 148739 View How can I load new protocols onto the QIAcube Connect MDx? 148740 View Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs? 148741 View Can the QIAcube Connect MDx centrifuge be used independently from protocol runs? 148742 View What dedicated QIAcube Kits are available? 148743 View Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument? 148744 View Can I run life science and custom protocols on a QIAcube Connect MDx? 148745 View What are the heating/cooling rates in the QIAquant instruments? 153919 View Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results? 153920 View What sample volume is suitable for use in the QIAquant? 153921 View What types of reaction vessels are required for use in the QIAquant? 153922 View Is the QIAquant equipped with a temperature gradient function? 153923 View Are the 96- and 384-sample blocks interchangeable? 153924 View Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel? 153925 View How many molecules can be detected in the system? 153926 View What applications can the QIAquant be used for? 153927 View Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels? 153928 View Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument? 153929 View Is the software of the QIAquant compatible with 21CFR part11? 153930 View Is it possible to import a standard curve from a previous run? 153947 View Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running? 153948 View Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384? 153950 View Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously? 153951 View Is it possible to establish an internet connection with QIAquant instrument? 153952 View Can I connect more than one QIAquant instrument to the same computer? 153953 View What is the algorithm behind data normalization in the QIAquant Software? 153954 View Is it possible to edit the sample sheet after the run in the QIAquant Software? 153955 View What are the LIMS capacity of the software? 153949 View Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export? 153946 View I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them? FAQ ID - 3560 View Where can I find ScreenGel profiles for the Large Fragment Marker Kit? 154189 View Do I need to dilute the size marker? 154191 View Can I start a QIAxcel run without using a process profile? 154188 View There is n/a as peak assignment instead of the size of my fragments. How can I solve this? 154190 View Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing? 153891 View What are recommended stopping points in the procedure of the QIAamp DNA FFPE Advanced Kits? 153892 View Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure? 153893 View Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C? 153894 View Can the 2nd Prot K step be omitted? 153896 View What size of DNA can be expected? 153897 View What is the difference of buffer FTB versus Buffer ATL? 153895 View Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments? 3761 View How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite? 3762 View Can I see error codes on the instrument touchscreen? 3763 View How are results stored within the QIAcuity Software Suite? 3764 View What is the scope of a regular maintenance of the QIAcuity? 3765 View Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex? 3766 View Can I use an empty nanoplate without seal to perform a dry run? 3767 View Does an incorrect handling of a plate might affect the results? 3768 View What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF? 3769 View Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not? 3770 View Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020? 3771 View How can I get the latest VPF (Volume Precision Factor)? 3772 View Where can I find assays specifically designed for QIAcuity? 3773 View Can I prepare a dPCR reaction directly in QIAcuity Nanoplate? 3774 View Can I use the dPCR master mix in a qPCR cycler for optimization purposes? 3775 View What is the purpose of the reference channel in QIAcuity? 3776 View How to prepare DNA prior to dPCR? 3778 View Can I use a custom master mix instead of a QIAGEN master mix? 3777 View Which sample preparation method is recommended for QIAcuity dPCR? 3779 View What are the storage conditions and expiry date of QIAcuity consumables? 3780 View What is the input volume for QIAcuity Nanoplate well? 3782 View Can I use the QIAcuity Nanoplate in more than one runs? 3781 View What is the VPF (Volume Precision Factor)? 3784 View When and how often do I need a new VPF (Volume Precision Factor)? 3785 View Is a standard curve needed in dPCR? 3786 View Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design? 3788 View Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine? 3791 View Is there something to consider when working with transduced cells treated with Polybrene? 3790 View For sequencing application, does the lysate require purification? 3792 View Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated? 3793 View Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing? 3794 View Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol? 3795 View What is the minimum cell number needed for cell lysis? 3789 View For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided? 3796 View What is the sequence of the QIAseq miRNA NGS 5’ Adapter? FAQ ID - 3672 View What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits? 3753 View Are there other options for paraffin removal? 3754 View Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure? 3755 View Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C? 3756 View What is the difference between Buffer FTB and Buffer ATL? 3757 View Can the 2nd Proteinase K step be omitted? 3758 View What size of DNA can be expected? 3759 View Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing? 3760 View Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis? FAQ ID -1056 View 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 View Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 View Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 View When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 View What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 View Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 View I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 View What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 View What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 View Do you sell CoralLoad dye separately? FAQ ID - 3368 View Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 View What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 View Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 View Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 View Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 View Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 View Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 View What is the RNase A concentration and composition of Buffer P1? FAQ ID -198 View Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)? 3797 View Where can I download the protocol for the KingFisher Flex? 3798 View Who can help in case of issues with the third party instrument? 3799 View Is the virus inactivated during the procedure? 3800 View Can I use sample volumes other than 300 µl? 3801 View Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry? 3802 View I would like to use the kit on another third party instrument. Who should I contact? 3803 View How long does a saliva collection take? 3804 View What can I do to stimulate saliva flow? 3805 View Can I stop saliva collection before the fill line is reached? 3806 View What can I do if I don't see the fill line anymore due to saliva bubbles? 3807 View What happens if I accidentally unscrew the funnel before saliva collection is completed? 3808 View May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely? 3809 View What is in the additive in the PAXgene Saliva Collector? 3810 View What is the shelf life of unused PAXgene Saliva Collectors? 3811 View How should unused PAXgene Saliva Collector be stored? 3812 View What should I avoid before sample collection? 3813 View How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva? 3814 View How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature? 3815 View Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C? 3816 View Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C? 3817 View Is RNA preserved in saliva collected in PAXgene Saliva Collector? 3818 View Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector? 3819 View What should I do if stabilized saliva samples are very heterogenous? 3820 View What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector? 3823 View Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument? 3824 View Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation? 3826 View Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector? 3827 View Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector? 3828 View Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity? 3829 View Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack? 3825 View What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector? 3822 View Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector? 3821 View What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments? FAQ ID - 3837 View Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits? FAQ ID - 3836 View What is the sequence of the UDI 5' Adapter? FAQ ID - 3835 View Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit? FAQ ID - 3674 View What can be used to validate results obtained with the QIAseq miRNA Library Kit? FAQ ID - 3675 View What is the maximum number of available sample indexes? FAQ ID - 3665 View What are recommended stopping points during the QIAseq miRNA Library Kit procedure? FAQ ID - 3663 View Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit? FAQ ID - 3659 View What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit? FAQ ID - 3668 View What is the acceptable number of reads per sample per miRNA sequencing run? FAQ ID - 3673 View Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays? FAQ ID -1169 View Is training of lab personnel included with the purchase of a BioRobot EZ1? FAQ ID -1239 View Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot? FAQ ID -1240 View How can we get our BioRobot EZ1 upgraded when new protocols are launched? FAQ ID -1241 View Where can I find information about the worktable setup on the BioRobot EZ1? FAQ ID -1243 View What disposables are required for nucleic acid isolations on the BioRobot EZ1? FAQ ID -1246 View How should I prepare buffy coat samples for use on the BioRobot EZ1? FAQ ID -1249 View Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1? FAQ ID -985 View What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer? FAQ ID -528 View Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1? FAQ ID -975 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1? FAQ ID -976 View Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1? FAQ ID -992 View Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)? FAQ ID -1025 View What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)? FAQ ID - 3838 View What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-? FAQ ID - 3839 View What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-? FAQ ID - 3840 View What PCR enzymes can be used following the first-strand synthesis? FAQ ID - 3841 View What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)? FAQ ID - 3842 View What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24? 2857 View Does the PyroMark LINE assay target all LINE sequences? 2860 View What is the amplicon length of the PyroMark CpG LINE assay? 2856 View How do I perform the PyroMark Q24 Advanced upgrade? FAQ ID -3163 View Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system? FAQ ID -3160 View What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents? FAQ ID -3165 View How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094 View We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions? FAQ ID - 3621 View What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855 View How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24? FAQ ID -2095 View How long does a single run on the PyroMark Q24 take? FAQ ID -2096 View 3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram FAQ ID - 3843 View When do I have to change the pulse settings/methods in a pyrosequencing run setup? FAQ ID -2942 View What is a PyroMark instrument method or instrument code? FAQ ID -2941 View Can the PyroMark Q96 CpG LINE assay be used with an ID system? 2861 View How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused? FAQ ID -2863 View How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage. FAQ ID -2878 View Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872 View What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875 View Is the CpG software included in the PyroMark instruments to study methylation status? FAQ ID -2842 View What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881 View Where can I find explanations to the warning given by the PyroMark software after run data analysis? FAQ ID -2874 View Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838 View How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations? FAQ ID -2848 View What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837 View What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879 View How many CpG sites are analyzed by the PyroMark CpG LINE assay? 2858 View Is there a user manual available for the PyroMark Assay design software? FAQ ID -2851 View Which operating system is compatible with PyroMark IdentiFire Software? FAQ ID - 3340 View How do I set up a PyroMark CpG Assay? FAQ ID -2814 View What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007 View Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823 View Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818 View How are the PyroMark CpG Assays reconstituted? FAQ ID -2817 View How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816 View What are the features of PyroMark CpG Assays, for example, in terms of design and validation? FAQ ID -2821 View What is included in a PyroMark Custom Assay? FAQ ID -2815 View In which format can PyroMark CpG Assays be ordered? FAQ ID -2824 View Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822 View Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862 View What is the recommended amplicon size for CpG assays? FAQ ID -2825 View What kind of reading length can I expect when using pyrosequencing technology for sequence analysis? FAQ ID -2216 View Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843 View Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832 View What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826 View What is the concentration of PyroMark Control Oligo? FAQ ID -2846 View What is the sample throughput of pyrosequencing systems? FAQ ID -2215 View How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877 View What is the sensitivity limitation for pyrosequencing? FAQ ID -2840 View What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852 View Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839 View How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871 View Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850 View What are critical steps during CTC enrichment? 3830 View How pure are the CTCs enriched from blood samples using the AdnaTest? 3831 View Can frozen blood be used for the procedure? 3832 View Can the EZ2 AdnaTest be used for other body fluids than blood? 3833 View Can Heparin blood be used? 3834 View Can the kit be used on the EZ2 Connect? FAQ ID - 3845 View Can I use a different elution buffer? FAQ ID - 3847 View What is the average amount of cfDNA present in 1 ml plasma? FAQ ID - 3846 View Can the kit be used on the EZ1 Advanced XL? FAQ ID - 3844 View Do you have a protocol for purification of cytoplasmic RNA from animal cells? FAQ ID -1257 View What kits does QIAGEN offer to extract RNA from whole blood? FAQ ID -304 View What is the composition of Buffer RLT? FAQ ID -2793 View What is the key technical challenge in isolating high quality RNA from cell or tissue samples? FAQ ID -2656 View Do you have a protocol for the isolation of RNA from leukocytes in milk? FAQ ID -952 View What is the maximum binding capacity of RNeasy spin columns? FAQ ID -290 View Can QIAquick Kits be used to clean up RNA samples? FAQ ID -490 View Are RNeasy spin columns sold separately? FAQ ID -159 View Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution? FAQ ID -619 View Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues? FAQ ID -467 View How can I minimize degradation of RNA from my pancreas sample? FAQ ID -624 View What is the composition of Buffer RW1? FAQ ID -2796 View Do you have a protocol for purification of total DNA from crude lysates? FAQ ID -1255 View Do you have a protocol for isolation of genomic DNA from saliva and mouthwash? FAQ ID -917 View Do you have a protocol for purification of total DNA from yeast? FAQ ID -1253 View Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser? FAQ ID -924 View Do you have a protocol for purification of total DNA from insects? FAQ ID -1254 View How can I precipitate genomic DNA using isopropanol? FAQ ID -2953 View What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)? FAQ ID - 3447 View Do you have a protocol for the isolation of genomic DNA from sperm? FAQ ID -909 View What is the composition of buffer AE? FAQ ID -730 View How do I safely inactivate biohazardous flow-through material? FAQ ID -12 View What is the advantage of running an analytical gel with fractions of my plasmid preparation? FAQ ID -769 View What is the recipe for LB? FAQ ID -212 View How do I know if my plasmid is a high- or low copy number type? FAQ ID -350 View Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription? FAQ ID -366 View I left Buffer P1 at room temperature after addition of RNase A, what shall I do? FAQ ID -859 View How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305 View With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns? FAQ ID -864 View What is the recipe for 2x YT? FAQ ID -213 View What are the additional plasmid bands I see on my gel? FAQ ID -1059 View Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis? FAQ ID -896 View What is the composition of Buffer PB? FAQ ID -2791 View Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit? FAQ ID -3102 View Why is my plasmid DNA yield low? FAQ ID -768 View Why do I get genomic DNA contamination in my plasmid prep? FAQ ID -353 View Do you have a protocol for the isolation of plasmid DNA from Agrobacterium? FAQ ID -898 View I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that? FAQ ID -1045 View What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent? FAQ ID -861 View What is the recipe for SOC medium? FAQ ID -798 View How much RNA does a typical mammalian cell contain? FAQ ID -2946 View Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage? FAQ ID -1037 View What are the effects of low A260/A230 ratios in RNA preparations on downstream applications? FAQ ID -2248 View What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728 View Do I need to purchase any consumable for processing the kit on EZ2 Connect? FAQ ID - 3849 View Are we able to customize protocol on the EZ2 Connect? FAQ ID - 3850 View Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit? FAQ ID - 3851 View Can the kit be run on EZ1 instruments? FAQ ID - 3852 View How can I check the integrity of RNA purified using RNeasy Kits? FAQ ID -1024 View What are the limits of detection of the QIAxcel Connect? FAQ ID - 3853 View When should the N2 bottle used with the QIAxcel Connect System be changed? FAQ ID - 3854 View What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID - 3855 View Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect? FAQ ID - 3856 View What are the differences between QIAxcel Advanced and QIAxcel Connect? FAQ ID - 3857 View Why does an intensity calibration of the gel cartridge for the QIAxcel Connect need to be performed? FAQ ID - 3858 View What is the QIAxcel Connect System? FAQ ID - 3859 View Can I combine your CGT dPCR assays with custom designed assays? FAQ ID - 3861 View How many assays can be multiplexed? FAQ ID - 3862 View In which channels can you detect the CGT dPCR assays? FAQ ID - 3863 View How can I resuspend the lyophilized assay? FAQ ID - 3864 View What quenchers are used? FAQ ID - 3865 View Do I need to use restriction enzymes when quantifying AAV genomes? FAQ ID - 3866 View Can the CGT dPCR assays also be used for the quantification of non-AAV samples? FAQ ID - 3868 View Can I use the CGT dPCR assays for the titer determination of RNA viruses? FAQ ID - 3869 View How do I know if the assays are compatible with the sample DNA of interest? FAQ ID - 3870 View Are the assays also compatible with qPCR? FAQ ID - 3871 View Can they be used with lentiviral vectors? FAQ ID - 3872 View Is restriction enzyme digestion required? FAQ ID - 3875 View dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter? FAQ ID - 3876 View My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate? FAQ ID - 3877 View Is dPCR sensitive to inhibition? FAQ ID - 3880 View Is DNA extraction from the samples needed? FAQ ID - 3873 View How much sample can I load for detecting host cell contamination? FAQ ID - 3879 View Where can I find a copy of the .kpf file for a BioSprint protocol? FAQ ID -3073 View Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730? FAQ ID -3075 View What is the minimum number of samples that can be extracted on a BioSprint 96? FAQ ID -3076 View Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored? FAQ ID -3077 View Can I use specific primers with the FastLane Kits? FAQ ID -3078 View Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C? FAQ ID -3079 View Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification? FAQ ID -3080 View Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect? FAQ ID - 3881 View Do we have specificity and sensitivity data for this kit? FAQ ID - 3882 View Do we have specificity and sensitivity data for this kit? FAQ ID - 3883 View Is it possible to scale up sample volume and reaction volume? FAQ ID - 3884 View What color channels are used? FAQ ID - 3885 View What is the percentage of false negative results that QIAGEN obtained during validations? FAQ ID - 3886 View What is the minimum and maximum concentration of effective RNA for the test? FAQ ID - 3887 View Anything important when starting the cycler run? FAQ ID - 3888 View Is it possible to use ABI instruments without ROX as a passive reference? FAQ ID - 3889 View What is the shelf-life of the Neo Assay Kit? FAQ ID - 3890 View Can the SARS-CoV-2 Neo Assay Kit detect all variants? FAQ ID - 3891 View Why did we not choose commonly used genes such as the S gene, E gene, or RdRP? FAQ ID - 3892 View Why did you choose ORF1a+ORF1b and ORF3a+ORF7a? FAQ ID - 3893 View Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay? FAQ ID - 3894 View Can I use the IC RNA of the QIAprep& Viral RNA UM Kit? FAQ ID - 3895 View Which positive control should I use? FAQ ID - 3896 View How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit? FAQ ID - 3897 View Which materials can be used as input samples for the QIAsymphony DSP Circulating DNA Kit? FAQ ID - 3702 View Are there important considerations for plasma generation and urine handling? FAQ ID - 3699 View How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature? FAQ ID - 3898 View How can PCR cycling conditions be optimized for Phoenix Hot Start Taq? FAQ ID - 3899 View Can Phoenix Hot Start Taq utilize cDNA as template for PCR? FAQ ID - 3900 View Is Phoenix Hot Start Taq capable of multiplex PCR? FAQ ID - 3901 View How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer? FAQ ID - 3902 View When should I use Phoenix Hot Start Taq GC reaction Buffer? FAQ ID - 3903 View What is the amplification length limit of Phoenix Hot Start Taq? FAQ ID - 3904 View What is the fidelity/error rate of Phoenix Hot Start Taq? FAQ ID - 3905 View Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage? FAQ ID - 3906 View How can yield for long targets be increased when using Phoenix Hot Start Taq? FAQ ID - 3907 View How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase? FAQ ID - 3908 View How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers? FAQ ID - 3909 View When should I use 5X VeraSeq GC Buffer? FAQ ID - 3910 View What is the amplification length limit of VeraSeq 2.0 DNA Polymerase? FAQ ID - 3911 View Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage? FAQ ID - 3913 View What is the amplification length limit of VeraSeq 2.0 DNA Polymerase? FAQ ID - 3912 View How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase? FAQ ID - 3914 View Will VeraSeq 2.0 DNA Polymerase incorporate dUTP? FAQ ID - 3915 View Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme? FAQ ID - 3916 View How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling? FAQ ID - 3917 View What denaturation temperature should be used in cycling conditions? FAQ ID - 3918 View What annealing temperature should be used in the cycling conditions? FAQ ID - 3919 View What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature? FAQ ID - 3920 View How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers? FAQ ID - 3922 View How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase? FAQ ID - 3921 View When should I use 5X VeraSeq GC Buffer? FAQ ID - 3923 View What is the amplification length limit of VeraSeq ULtra DNA Polymerase? FAQ ID - 3924 View What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase? FAQ ID - 3925 View How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase? FAQ ID - 3926 View Will VeraSeq ULtra DNA Polymerase incorporate dUTP? FAQ ID - 3927 View Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang? FAQ ID - 3928 View Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme? FAQ ID - 3929 View What denaturation temperature should be used in the cycling conditions? FAQ ID - 3931 View What annealing temperature should be used in the cycling conditions? FAQ ID - 3932 View What are the ResDNA Quant Kit components? FAQ ID - 3878 View How can I load new protocols onto the QIAcube? FAQ ID -1421 View Are run reports and/or log files available on the QIAcube? FAQ ID -1412 View What applications are offered on the QIAcube? FAQ ID -1403 View Can the QIAcube heater/shaker be used independently from protocol runs? FAQ ID -1422 View What EZ1 DNA Investigator protocol is recommended for very precious samples? FAQ ID -1154 View When should the Normalization protocol be used? FAQ ID -1155 View What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap? FAQ ID - 3364 View How can I extract DNA from a polyacrylamide (PAGE) gel? FAQ ID -120 View Why does my DNA sample float out of the slot when loading it onto an agarose gel? FAQ ID -205 View What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick? FAQ ID -148 View I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost? FAQ ID -756 View Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels? FAQ ID -576 View Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL? FAQ ID -2032 View Can the QIAcube be connected to a laboratory information management system? FAQ ID -1411 View What are the dimensions and weight of the QIAcube? FAQ ID -1407 View What dedicated QIAcube Kits are available? FAQ ID -2337 View How can I decontaminate the QIAcube system? FAQ ID -1419 View Can the QIAcube rotor and/or buckets be removed for cleaning? FAQ ID -1410 View Can the QIAcube centrifuge be used independently from protocol runs? FAQ ID -1423 View Do I need to buy special kits for the QIAcube? FAQ ID -1402 View Do I need to discard partially used QIAcube tip racks? FAQ ID -1418 View Can I program my own protocols for the QIAcube? FAQ ID -1405 View Can the CGT dPCR assays also be used for the analysis of AAV genome integrity? FAQ ID - 3867 View Is the QIAprep&amp CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN? 3787 View Do you have a protocol for the isolation of viral RNA from stool? FAQ ID -918 View Which extraction kits are recommended? FAQ ID - 3874 View How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C? FAQ ID - 3934 View What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)? FAQ ID - 3935 View What is the amount of standard for the different kit? FAQ ID - 3936 View How did you calculate the conversion factor? FAQ ID - 3937 View What does the standard kit contain? FAQ ID - 3938 View What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)? FAQ ID - 3939 View How long are sample lysates stable in the fridge/ freezer? FAQ ID - 143765 View Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument? FAQ ID -2027 View Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP? FAQ ID -2031 View After bisulfite conversion, can the DNA be stored? FAQ ID -2409 View Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit? FAQ ID - 3940 View When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit? FAQ ID - 3941 View What size of RNA can be expected? FAQ ID - 3942 View Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit? FAQ ID - 3947 View When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit FAQ ID - 3948 View Are there other options for paraffin removal? FAQ ID - 3949 View What are recommended stopping points in the procedure of the EZ2 AllPrep DNA/RNA FFPE Kit? FAQ ID - 3950 View Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)? FAQ ID - 3951 View An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away. FAQ ID - 3952 View Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue? FAQ ID - 3953 View What size of RNA can be expected? FAQ ID - 3954 View Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID - 3955 View My overall RNA yield is fine but RT-PCR performance poor – how can I improve this? FAQ ID - 3956 View Why are my DV200 values of my RNA sample so low? FAQ ID - 3957 View The integrity of my DNA sample (e.g. QIAxcel Connect) is bad. FAQ ID - 3958 View How do I proceed if the amount of starting material cannot be calculated in detail? FAQ ID - 3943 View Are there other options for paraffin removal? FAQ ID - 3946 View Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID - 3944 View My overall RNA yield is fine but RT-PCR performance poor – how can I improve this? FAQ ID - 3945 View Is this available without glycerol? FAQ ID - 3959 View What types of priming are compatible with EnzScript M-MLV RT RNase H-? FAQ ID - 3961 View What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)? FAQ ID - 3964 View What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)? FAQ ID - 3960 View What PCR enzymes can be used following first-strand synthesis? FAQ ID - 3963 View Is the kit compatible with unpurified in-process samples? FAQ ID - 3965 View Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration? FAQ ID - 3971 View Do you recommend storage of highly diluted AAV samples? FAQ ID - 3972 View Is it possible to store the lysates? How can the lysates be stored? FAQ ID - 3973 View Can I use MspI or HpaII restriction enzymes from other suppliers? FAQ ID - 3975 View How many AAV genome copies can I load into the QIAcuity? FAQ ID - 3977 View Do you need to dilute the lysates before setting up the PCR mix? FAQ ID - 3978 View How do I know if the CGT dPCR assays are compatible with the sample DNA of interest? FAQ ID - 3980 View Are the CGT dPCR assays also compatible with qPCR? FAQ ID - 3981 View Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays? FAQ ID - 3982 View What is included in the SureSilencing shRNA Plasmids shipment? FAQ ID -2785 View Is the QIAxcel Advanced discontinued? FAQ ID - 3983 View What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit? FAQ ID - 3984 View What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits? FAQ ID - 3985 View What water should I use to prepare the buffer concentrates? FAQ ID - 3986 View What is the new Waste Tube made of? FAQ ID - 3987 View Are the QIAwave kits based on a different chemistry than the legacy kits? FAQ ID - 3990 View Is there a way to compare the environmental impacts of the kit? FAQ ID - 3991 View Can the QIAwave kits be recycled? FAQ ID - 3992 View Does the reuse of the Waste Tubes increase the risk of cross-contamination? FAQ ID - 3988 View I don’t have any glassware in the lab, is the QIAwave kit still a good option for me? FAQ ID - 3989 View Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument? FAQ ID - 3993 View Is the kit compatible with unpurified in-process samples? FAQ ID - 3994 View My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction? FAQ ID - 3995 View Can I use Internal Control as overall control? FAQ ID - 3996 View How long can I store the reconstituted Internal Control and the reconstituted Positive Control? FAQ ID - 3997 View Do you recommend storage of highly diluted mycoplasma samples? FAQ ID - 3998 View What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit? FAQ ID - 3999 View On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 View Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex? FAQ ID -2569 View Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System? FAQ ID -2571 View Which human target is used for the quantification in the Investigator Quantiplex Kit? FAQ ID -2577 View Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated? FAQ ID - 3728 View Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3? FAQ ID -2570 View Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit? FAQ ID - 3730 View Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit? FAQ ID - 3743 View Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit? FAQ ID - 3742 View Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System? FAQ ID - 3732 View How long does a run take? FAQ ID -2566 View Investigator Quantiplex: For which real-time cyclers has the kit been validated? FAQ ID -2567 View What are the storage conditions? FAQ ID - 3748 View Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit? FAQ ID -2568 View How can I streamline the quantification workflow? FAQ ID - 3738 View Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q? FAQ ID - 3729 View Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated? FAQ ID - 3741 View Can I optimize a dPCR assay on the QIAcuity using gradient temperature? FAQ ID - 3783 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits? FAQ ID -2126 View Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2130 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2131 View What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761 View Can I use REPLI-g for SNP Genotyping? FAQ ID -700 View Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit? FAQ ID -2058 View What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System? FAQ ID - 3506 View Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes? FAQ ID -2595 View Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays? FAQ ID -717 View Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes? FAQ ID -1979 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits? FAQ ID -1452 View How is "Touchdown PCR" used to increase PCR specificity? FAQ ID -75 View What should the starting template DNA quality and quantity be for PCR? FAQ ID -74 View What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit? FAQ ID -165 View Why do I get smeared PCR products? FAQ ID -87 View Does QIAGEN sell Q-Solution separately? FAQ ID -204 View Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? FAQ ID -1745 View Is Q-Solution required for PCR with QIAGEN's PCR kits? FAQ ID -380 View Have you tested the effect of inhibitors on PCR performance? FAQ ID -818 View What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits? FAQ ID -606 View Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility? FAQ ID - 3974 View What is the recommended HpaII concentration? FAQ ID - 3976 View Can I use the lysate from the CGT Viral Vector Lysis kit and combine it with another PCR system? FAQ ID - 3979 View Do you have any recommendations on how to process my AAV samples? FAQ ID - 3860 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care

Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?

FAQ ID - 3724

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Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?

FAQ ID - 3725

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Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?

FAQ ID - 3726

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Does QIAGEN offer an anti-GFP gapmer control?

FAQ ID - 3739

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Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?

FAQ ID - 3745

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How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?

FAQ ID - 3744

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Do I need to buy special kits for the QIAcube Connect?

FAQ ID - 141485

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Can I program my own protocols for the QIAcube Connect?

FAQ ID - 141486

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Can the QIAcube Connect rotor and/or buckets be removed for cleaning?

FAQ ID - 141491

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Can purification columns from other suppliers be processed on the QIAcube Connect?

FAQ ID - 141487

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How can I get software updates for the QIAcube Connect?

FAQ ID - 141494

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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?

FAQ ID - 141496

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Do I need to discard partially used QIAcube Connect tip racks?

FAQ ID - 141499

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How can I decontaminate the QIAcube Connect system?

FAQ ID - 141500

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Can the QIAcube Connect heater/shaker be used independently from protocol runs?

FAQ ID - 141502

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How can I load new protocols onto the QIAcube Connect?

FAQ ID - 141501

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What dedicated QIAcube Connect kits are available?

FAQ ID - 141504

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Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?

FAQ ID - 1414507

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Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?

FAQ ID - 141505

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Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?

FAQ ID - 141506

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Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?

FAQ ID - 141508

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How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?

FAQ ID - 141509

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Can the CB and RB sample tubes be used interchangeably?

FAQ ID - 141510

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Which network settings are supported by the QIAcube Connect and Q-Base?

FAQ ID - 141517

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Why can I not find the Q-Base device in the device list during the first installation?

FAQ ID - 141516

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Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?

FAQ ID - 1414518

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Why is the QIAcube Connect App reacting so slowly?

FAQ ID - 141519

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Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?

FAQ ID - 1414520

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Why does the connection test fail?

FAQ ID - 141522

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How critical is the time of the temperature change from 60°C to 80°C?

FAQ ID - 143768

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Is it possible to use Lyse&Spin baskets?

FAQ ID - 143762

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Why is DTT used only for semen samples?

FAQ ID - 143761

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Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?

FAQ ID - 143079

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Can the lysis volume be reduced for higher sensitivity?

FAQ ID - 143763

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What if only one heater shaker is available?

FAQ ID - 143764

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Why is there no male DNA obtained from sexual assault samples?

FAQ ID - 143767

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If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)

FAQ ID - 143766

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Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?

FAQ ID -147395

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What RNAs has QIAseq FastSelect been designed to remove?

FAQ ID -147910

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How does QIAseq FastSelect work?

FAQ ID -147907

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Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?

FAQ ID -147909

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Will QIAseq FastSelect work in other species?

FAQ ID -147911

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What library prep kits has QIAseq FastSelect been tested with?

FAQ ID -147912

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I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?

FAQ ID -147913

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What RNA range has the QIAseq FastSelect been tested with?

FAQ ID -147914

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Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?

FAQ ID -147915

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Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?

FAQ ID -147916

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Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?

FAQ ID -1

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How should I quantify RNA isolated with RNeasy Kits?

FAQ ID -32

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When is chloramphenicol amplification of plasmids performed?

FAQ ID -3

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What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?

FAQ ID -45

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What are the most commonly used protease inhibitors?

FAQ ID -53

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How should I propagate pQE expression plasmids?

FAQ ID -58

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How can I increase the amount of soluble recombinant protein in E. coli expression?

FAQ ID -64

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How can I remove imidazole from a protein sample?

FAQ ID -91

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Why does my isolated RNA have a low OD 260/280 ratio?

FAQ ID -97

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How can I avoid little or no RNA yields when using an RNeasy Kit?

FAQ ID -28

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How should I store plant material for DNA isolation using the DNeasy Plant Kit?

FAQ ID -114

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Do I need to use an RNase inhibitor in my RT reaction?

FAQ ID -119

View

Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?

FAQ ID -127

View

Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?

FAQ ID -130

View

How does imidazole affect my quantitation of protein?

FAQ ID -132

View

Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?

FAQ ID -133

View

What is the difference between disruption and homogenization in the RNeasy System?

FAQ ID -139

View

What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?

FAQ ID -142

View

Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?

FAQ ID -147

View

What is the genotype of the EZ Competent Cells?

FAQ ID -157

View

What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?

FAQ ID -160

View

What are the molecular weights of proteins in the 6xHis Protein Ladder?

FAQ ID -169

View

How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?

FAQ ID -176

View

How can I improve recoveries when using the QIAquick Kits?

FAQ ID -180

View

How can I improve transfection efficiency using Effectene Transfection Reagent?

FAQ ID -181

View

What is the principle behind Effectene Transfection Reagent?

FAQ ID -184

View

What is the composition of Buffer EB?

FAQ ID -199

View

Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?

FAQ ID - 3709

View

Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?

FAQ ID - 3711

View

What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?

FAQ ID - 3715

View

Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?

FAQ ID - 3716

View

How can I check for purity of RNA isolated using RNeasy Kits?

FAQ ID -1023

View

What is the recipe for 1x PBS solution?

FAQ ID -1030

View

Where can I find a protocol for cleanup of already purified plasmid DNA?

FAQ ID -1031

View

How do I perform an Acetone Precipitation for concentrating and desalting protein samples?

FAQ ID -1035

View

What is the difference between Buffers RLT and RLT Plus?

FAQ ID -1043

View

What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?

FAQ ID -1047

View

Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?

FAQ ID -1051

View

Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?

FAQ ID -1052

View

How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?

FAQ ID -1061

View

What should I use as a standard for absolute quantification in real-time PCR?

FAQ ID -1085

View

What does transcript variant mean when searching for specific QuantiTect Primer Assays?

FAQ ID -1137

View

What is a QuantiTect Primer Assay?

FAQ ID -1141

View

Do QuantiTect Primer Assays contain SYBR Green dye?

FAQ ID -1143

View

What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?

FAQ ID -1218

View

What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?

FAQ ID -1220

View

What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?

FAQ ID -1221

View

Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?

FAQ ID -1250

View

Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?

FAQ ID -1251

View

What is the pH value of QIAGEN's Nuclease-Free Water?

FAQ ID -1290

View

Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?

FAQ ID -1327

View

Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?

FAQ ID -1328

View

Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?

FAQ ID -1352

View

How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?

FAQ ID -1354

View

What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?

FAQ ID -1356

View

How do I resuspend my FlexiPlate siRNAs?

FAQ ID -1360

View

Are there any specific cleaning solutions recommended for the QIAcube?

FAQ ID -1416

View

Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?

FAQ ID -1450

View

Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1577

View

What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1580

View

What is the binding capacity of the Ni-NTA Superflow Cartridges?

FAQ ID -1603

View

How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?

FAQ ID -1644

View

Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?

FAQ ID -1659

View

Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?

FAQ ID -1664

View

What is the composition of Buffer PE?

FAQ ID -1691

View

What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?

FAQ ID -1738

View

How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?

FAQ ID -1838

View

Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?

FAQ ID -1848

View

What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID -1852

View

If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

FAQ ID -1858

View

What are the dimensions and weight of the QIAsymphony SP System?

FAQ ID -1901

View

Are there any specific cleaning solutions recommended for the QIAsymphony SP?

FAQ ID -1913

View

How much sample can be used with the RNeasy Plus 96 Kit?

FAQ ID -1992

View

What is the composition of Buffer P2?

FAQ ID -203

View

A white precipitate has formed in my 10x RT buffer. Is it still ok to use?

FAQ ID -216

View

For how long are BioMag Particles stable?

FAQ ID -262

View

I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?

FAQ ID -263

View

Can I clean up my DNase treated RNA samples using RNeasy columns?

FAQ ID -286

View

How can one determine the optimal annealing temperature for a specific PCR assay?

FAQ ID -288

View

What is the composition of the QIAGEN Multiplex PCR Buffer?

FAQ ID -289

View

Can I use HEPES buffer instead of phosphate in my Ni-NTA column?

FAQ ID -291

View

Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?

FAQ ID -293

View

What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?

FAQ ID -2004

View

What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

FAQ ID -2029

View

How can a C-terminal 6xHis-tagged QIAgene be expressed?

FAQ ID -2041

View

Why is there more than one QIAgene for my gene of interest?

FAQ ID -2044

View

Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

FAQ ID -2079

View

Does the Rotor-Gene Q software have the capacity to export data?

FAQ ID -2089

View

Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?

FAQ ID -2122

View

Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2163

View

Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?

FAQ ID -2190

View

How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?

FAQ ID -2192

View

Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?

FAQ ID -2196

View

Why do I need normalization using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2198

View

What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2203

View

What buffer conditions give the best resolution for agarose gel electrophoresis?

FAQ ID -2256

View

How much DNA should be loaded per well of an agarose gel?

FAQ ID -2259

View

Why do I have wavy DNA bands on my agarose gel?

FAQ ID -2260

View

What is the "seed sequence" of a miRNA?

FAQ ID -2262

View

Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?

FAQ ID -2301

View

Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?

FAQ ID -2315

View

How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?

FAQ ID -2340

View

How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?

FAQ ID -2341

View

How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?

FAQ ID -2350

View

Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?

FAQ ID -2371

View

Can I buy QIAquick and MinElute columns separately?

FAQ ID -2460

View

What is the PAXgene 96 filter plate?

FAQ ID -2486

View

Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?

FAQ ID -2509

View

Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?

FAQ ID -2514

View

How long is the fixation time?

FAQ ID -2519

View

What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?

FAQ ID -2543

View

Are there any accessories required for sample disruption with the TissueLyser LT?

FAQ ID -2551

View

Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?

FAQ ID -2603

View

During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit

FAQ ID -2607

View

What is the SBTengine® software?

FAQ ID -2628

View

How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?

FAQ ID -2654

View

What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?

FAQ ID -2659

View

How can I avoid or remove genomic DNA contamination from the total RNA preparation?

FAQ ID -2662

View

What are the differences between one-step and two-step RT--PCR?

FAQ ID -2666

View

What is qPCR?

FAQ ID -2668

View

What are the common primer and probe chemistries utilized for qPCR assays?

FAQ ID -2669

View

What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?

FAQ ID -2671

View

What negative controls are typically included in qPCR and/or qRT-PCR experiments?

FAQ ID -2672

View

What positive controls are typically included in qPCR and/or qRT-PCR experiments?

FAQ ID -2673

View

What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?

FAQ ID -2674

View

Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?

FAQ ID -2675

View

How does HotStart PCR help minimize nonspecific amplification events?

FAQ ID -2676

View

What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?

FAQ ID -2678

View

What is the delta Rn value?

FAQ ID -2681

View

What is the threshold cycle or Ct value?

FAQ ID -2682

View

Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?

FAQ ID -2684

View

Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?

FAQ ID -2685

View

Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?

FAQ ID -2686

View

Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?

FAQ ID -2689

View

Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?

FAQ ID -2690

View

What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?

FAQ ID -2691

View

What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?

FAQ ID -2692

View

What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?

FAQ ID -2693

View

How do I determine the amplification efficiency of my qPCR assay?

FAQ ID -2694

View

How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?

FAQ ID -2696

View

What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?

FAQ ID -2705

View

What do I need to complete a RT² qPCR Primer Assay?

FAQ ID -2707

View

How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?

FAQ ID -2729

View

What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?

FAQ ID -2739

View

How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?

FAQ ID -2749

View

How can I identify the binding sites of my transcription factor using ChIP?

FAQ ID -2753

View

At what point can I stop and freeze my samples when using ChIP?

FAQ ID -2754

View

What is RNA interference (RNAi)?

FAQ ID -2755

View

What are the key factors for success in an RNAi experiment?

FAQ ID -2758

View

What criteria should one use in choosing between siRNA versus shRNA for their studies?

FAQ ID -2771

View

How does stable transfection of a shRNA-encoding plasmid work?

FAQ ID -2779

View

What are the SureSilencing shRNA Plasmids?

FAQ ID -2784

View

What is the composition of Buffer S3?

FAQ ID -2788

View

What is the composition of Buffer ETR?

FAQ ID -2789

View

What is the composition of Buffer BB?

FAQ ID -2790

View

What is the composition of Buffer RLT plus?

FAQ ID -2794

View

What is the composition of Buffer RLC?

FAQ ID -2795

View

What is the composition of Buffer RPE?

FAQ ID -2797

View

What is the composition of Buffer RWT?

FAQ ID -2798

View

What is the composition of gDNA Eliminator Solution?

FAQ ID -2799

View

What is the composition of Buffer RDD?

FAQ ID -2800

View

What is the composition of Buffer PKD?

FAQ ID -2801

View

What is the composition of Buffer ALO?

FAQ ID -2805

View

What is the composition of RBC Lysis Solution?

FAQ ID -2808

View

What is the composition of Cell Lysis Solution?

FAQ ID -2809

View

What is the composition of Protein Precipitation Solution?

FAQ ID -2810

View

What is the composition of Cell Suspension Solution?

FAQ ID -2811

View

What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?

FAQ ID -2812

View

What is the composition of DNA Hydration Solution in Gentra Puregene Kits?

FAQ ID -2813

View

What is the meaning of the abbreviations NDT and CDT and what are the differences?

FAQ ID -2841

View

What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?

FAQ ID -2849

View

Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?

FAQ ID -2894

View

Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

FAQ ID -2926

View

What is the composition of Buffer G2?

FAQ ID -2943

View

What is the composition of Buffer B1?

FAQ ID -2944

View

What is the composition of Buffer B2?

FAQ ID -2945

View

How much DNA does a human cell contain?

FAQ ID -2948

View

How much RNA does a bacterial cell contain?

FAQ ID -2949

View

How much DNA and RNA can be expected from human blood cells?

FAQ ID -2950

View

What is the cellular composition of human blood?

FAQ ID -2951

View

How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?

FAQ ID -2954

View

Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?

FAQ ID -2980

View

Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?

FAQ ID -2987

View

What is the sequence of the miScript Universal Primer?

FAQ ID -2994

View

How can I keep my centrifuge tubes endotoxin-free?

FAQ ID -301

View

Can Buffers N3 and P3 be used interchangeably?

FAQ ID -310

View

Are QIAprep and QIAquick Spin columns interchangeable?

FAQ ID -311

View

Can I store agarose gel slices containing DNA for gel extraction at a later point?

FAQ ID -313

View

How can QIAGEN Protease and Proteinase K be inactivated?

FAQ ID -315

View

Does the 5x OneStep RT-PCR Buffer contain BSA?

FAQ ID -326

View

What is the origin of replication and the plasmid copy number of the pQE vectors?

FAQ ID -338

View

Is QIAGEN carrier RNA sold separately?

FAQ ID -351

View

What is the white insoluble precipitate in my resuspended plasmid DNA pellet?

FAQ ID -352

View

What is the composition of PBS?

FAQ ID -361

View

How can I express toxic protein in E. coli?

FAQ ID -373

View

How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

FAQ ID -374

View

What are the dissociation constants for the Anti-His Antibodies?

FAQ ID -385

View

What is the average molecular weight of a siRNA, and how do I convert uM to ug values?

FAQ ID -388

View

Can siRNA silence bacterial genes?

FAQ ID -395

View

How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?

FAQ ID -3003

View

Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?

FAQ ID -3032

View

Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?

FAQ ID -3053

View

Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?

FAQ ID -3054

View

What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?

FAQ ID -3055

View

What is the thermal output for a BioRobot MDx and BioRobot Universal?

FAQ ID -3056

View

What is the thermal output for a QIAsymphony SP and AS instrument?

FAQ ID -3057

View

How precise is the QIAgility?

FAQ ID -3063

View

What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?

FAQ ID -3064

View

How long does it take to set up a 96-well plate PCR reaction on the QIAgility?

FAQ ID -3066

View

Can I use a bar code scanner or reader on the QIAgility?

FAQ ID -3067

View

How can I find the run parameters for a BioSprint protocol that I am running?

FAQ ID -3069

View

Can BioSprint 96 be connected to LIMS?

FAQ ID -3070

View

What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?

FAQ ID -3081

View

What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?

FAQ ID -3082

View

What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?

FAQ ID -3083

View

What is the concentration of the different Proteinase K solutions sold by QIAGEN?

FAQ ID -3084

View

Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?

FAQ ID -3085

View

What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?

FAQ ID -3086

View

Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?

FAQ ID -3088

View

What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?

FAQ ID -3096

View

How to upload a Flexiplate on QIAGEN Webpage?

FAQ ID -3103

View

Can we do modification XXXXX for custom siRNA that is not on the web page?

FAQ ID -3107

View

What is the stability of siRNA lyophilized and resuspended?

FAQ ID -3109

View

Do you offer FlexiTube or FlexiPlate siRNAs for rat?

FAQ ID -3114

View

Is it possible to extract RNA from frozen blood?

FAQ ID -3119

View

Is it possible to extract RNA from RNAprotect-stabilized-blood?

FAQ ID -3120

View

What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails

-48

View

Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?

FAQ ID -3125

View

Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?

FAQ ID -3132

View

How do I remove the RNA contamination from purified DNA?

FAQ ID -3133

View

What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?

FAQ ID -3140

View

What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?

FAQ ID -3169

View

What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?

FAQ ID -3174

View

What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?

FAQ ID -3175

View

What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?

FAQ ID -3178

View

Is REST software Windows 7 compatible?

FAQ ID -3182

View

Can the Rotor-Gene Q perform gradient PCR?

FAQ ID -3183

View

What is the expected amplicon size of the Microbial DNA qPCR Assays?

FAQ ID — 3396

View

What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?

FAQ ID — 3397

View

What is LLOQ?

FAQ ID — 3401

View

What is the difference between LLOQ and LOD?

FAQ ID — 3402

View

What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?

FAQ ID - 3427

View

What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?

FAQ ID - 3429

View

Can the miScript II RT Kit be used to quantify plant miRNAs?

FAQ ID - 3431

View

What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?

FAQ ID - 3444

View

Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?

FAQ ID - 3467

View

What is the proper disposal procedure for waste from the sample preparation?

FAQ ID - 3470

View

What is the expected yield from 8.5 ml of blood?

FAQ ID - 3496

View

What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?

FAQ ID - 3501

View

How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

FAQ ID - 3507

View

How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

FAQ ID - 3508

View

What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?

FAQ ID - 3510

View

Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?

FAQ ID - 3512

View

What does PAXgene Tissue STABILIZER contain?

FAQ ID - 3513

View

What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?

FAQ ID - 3516

View

Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?

FAQ ID - 3517

View

What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?

FAQ ID - 3519

View

Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?

FAQ ID - 3521

View

Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?

FAQ ID - 3534

View

Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?

FAQ ID - 3559

View

Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?

FAQ ID - 3573

View

Is clot detection possible on the QIAsymphony?

FAQ - ID 3588

View

How can I determine if a tube is suitable for clot detection on QIAsymphony?

FAQ ID - 3590

View

How should I thaw frozen plasma samples?

FAQ ID - 3645

View

How do Unique Molecular Indexes (UMIs) improve quantification?

FAQ ID - 3669

View

Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?

FAQ ID - 3686

View

I would like to stop during the library preparation procedure. When can I do that?

FAQ ID - 3687

View

Which regions are covered by the GR DNAseq V2 Panels (#181900)?

FAQ ID - 3688

View

When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?

FAQ ID - 3690

View

How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?

FAQ ID - 3691

View

Are peak heights of Q48 comparable to other PyroMark instruments?

FAQ ID - 3692

View

How much space does the PyroMark Q48 Autoprep instrument need?

FAQ ID - 3693

View

Can unused wells in a pyrosequencing discs be used in the next run?

FAQ ID - 3694

View

Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?

FAQ ID - 3695

View

What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?

FAQ ID - 3696

View

What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?

FAQ ID - 3698

View

How do I prepare Buffer MP?

FAQ ID - 3620

View

Are the QIAshredder columns included in various QIAGEN kits the same?

FAQ ID - 3624

View

What is the composition of buffer QBT?

FAQ ID -411

View

What is the composition of buffer QC?

FAQ ID -412

View

What is the composition of buffer QF?

FAQ ID -413

View

What is the composition of buffer STE?

FAQ ID -415

View

What is the composition of buffer TE?

FAQ ID -416

View

What is the composition of buffer FWB2?

FAQ ID -417

View

What is the composition of buffer P3?

FAQ ID -418

View

What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?

FAQ ID -470

View

Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?

FAQ ID -473

View

Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?

FAQ ID -474

View

Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?

FAQ ID -482

View

Can Ni-NTA resins be used to purify protein with an internal His-tag?

FAQ ID -496

View

How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?

FAQ ID -498

View

Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?

FAQ ID -500

View

Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?

FAQ ID -518

View

What is the composition of the siRNA resuspension & annealing buffer?

FAQ ID -522

View

Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?

FAQ ID -523

View

What is Tissue-Tek O.C.T., and what is it used for?

FAQ ID -530

View

Why do replicates in real-time PCR have different plateau heights?

FAQ ID -539

View

How can I avoid primer-dimer formation during PCR amplification?

FAQ ID -544

View

How can I tell if I have primer-dimers in my PCR reaction?

FAQ ID -552

View

How should I store cDNA produced using Omniscript Reverse Transcriptase?

FAQ ID -561

View

Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?

FAQ ID -563

View

What is the composition of Buffer ER? Is it available separately?

FAQ ID -571

View

What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?

FAQ ID -572

View

Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?

FAQ ID -575

View

Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?

FAQ ID -577

View

What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?

FAQ ID -600

View

Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?

FAQ ID -603

View

What do you recommend for the cleanup of genomic DNA (gDNA)?

FAQ ID -618

View

How can I improve my RNA yield from liver sample when using RNeasy Kits?

FAQ ID -623

View

Why do you recommend using Triton X for the purification of 6xHis-tagged protein?

-100

View

What 96-well plates do you recommend for use with the LiquiChip Workstation?

FAQ ID -630

View

What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?

FAQ ID -631

View

Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?

FAQ ID -633

View

Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?

FAQ ID -637

View

What is the shelf-life of my QIAGEN Kit?

FAQ ID -651

View

What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?

FAQ ID -665

View

Can I purchase Phi29 DNA polymerase only?

FAQ ID -708

View

What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?

FAQ ID -721

View

I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?

-20

View

I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?

FAQ ID -745

View

How much DNA is obtained in the average PCR reaction?

FAQ ID -750

View

Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?

FAQ ID -754

View

Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?

FAQ ID -759

View

How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?

FAQ ID -762

View

What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?

FAQ ID -764

View

What is the composition of Buffer N3?

FAQ ID -767

View

Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?

FAQ ID -770

View

How should fluorescent labeled probes be stored?

FAQ ID -784

View

Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?

FAQ ID -785

View

Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?

FAQ ID -786

View

How can I get specific information and a quote for my individual Sequencing Service needs?

FAQ ID -792

View

Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?

FAQ ID -795

View

How can I separate PCR fragments that are small and very close in size on an agarose gel?

FAQ ID -800

View

Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?

FAQ ID -802

View

What Disinfectant Solution do you recommend for the BioRobot MDx?

FAQ ID -809

View

Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?

FAQ ID -842

View

What annealing temperature should be used with the QuantiTect Primer Assays?

FAQ ID -849

View

Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?

FAQ ID -860

View

Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?

FAQ ID -865

View

Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?

FAQ ID -879

View

Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

FAQ ID -900. Test

View

Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?

FAQ ID -907

View

Do you have a protocol for the isolation of genomic DNA from bone?

FAQ ID -908

View

What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?

FAQ ID -942

View

Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?

FAQ ID -959

View

Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?

FAQ ID -971

View

Do you have a protocol for the purification of PCR products using the BioSprint System?

FAQ ID -984

View

Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

FAQ ID -997

View

How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?

FAQ ID - 3707

View

How can I increase expression of my 6xHis-tagged protein in E. coli?

FAQ ID -63

View

Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?

148723

View

What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?

148724

View

What is the composition of buffer OW2?

FAQ ID -419

View

What is the composition of buffer OBB?

FAQ ID -421

View

Do I need to buy special kits for the QIAcube Connect MDx?

148725

View

What applications are offered on the QIAcube Connect MDx instrument?

148726

View

Can I program my own protocols for the QIAcube Connect MDx?

148727

View

Can purification columns from other suppliers be processed on the QIAcube Connect MDx?

148728

View

What are the dimensions and weight of the QIAcube Connect MDx?

148729

View

Can the QIAcube rotor adapters be autoclaved?

148730

View

Can the QIAcube rotor and/or buckets be removed for cleaning?

148731

View

Can the QIAcube be connected to a laboratory information management system?

148732

View

Are run reports and/or log files available on the QIAcube?

148733

View

How can I get software updates for the QIAcube Connect MDx?

148734

View

Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion?

148735

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

148736

View

Do I need to discard partially used QIAcube tip racks?

148738

View

How can I decontaminate the QIAcube Connect MDx system?

148739

View

How can I load new protocols onto the QIAcube Connect MDx?

148740

View

Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs?

148741

View

Can the QIAcube Connect MDx centrifuge be used independently from protocol runs?

148742

View

What dedicated QIAcube Kits are available?

148743

View

Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?

148744

View

Can I run life science and custom protocols on a QIAcube Connect MDx?

148745

View

What are the heating/cooling rates in the QIAquant instruments?

153919

View

Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results?

153920

View

What sample volume is suitable for use in the QIAquant?

153921

View

What types of reaction vessels are required for use in the QIAquant?

153922

View

Is the QIAquant equipped with a temperature gradient function?

153923

View

Are the 96- and 384-sample blocks interchangeable?

153924

View

Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel?

153925

View

How many molecules can be detected in the system?

153926

View

What applications can the QIAquant be used for?

153927

View

Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels?

153928

View

Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument?

153929

View

Is the software of the QIAquant compatible with 21CFR part11?

153930

View

Is it possible to import a standard curve from a previous run?

153947

View

Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?

153948

View

Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384?

153950

View

Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?

153951

View

Is it possible to establish an internet connection with QIAquant instrument?

153952

View

Can I connect more than one QIAquant instrument to the same computer?

153953

View

What is the algorithm behind data normalization in the QIAquant Software?

153954

View

Is it possible to edit the sample sheet after the run in the QIAquant Software?

153955

View

What are the LIMS capacity of the software?

153949

View

Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export?

153946

View

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

FAQ ID - 3560

View

Where can I find ScreenGel profiles for the Large Fragment Marker Kit?

154189

View

Do I need to dilute the size marker?

154191

View

Can I start a QIAxcel run without using a process profile?

154188

View

There is n/a as peak assignment instead of the size of my fragments. How can I solve this?

154190

View

Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing?

153891

View

153892

View

Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure?

153893

View

Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C?

153894

View

Can the 2nd Prot K step be omitted?

153896

View

What size of DNA can be expected?

153897

View

What is the difference of buffer FTB versus Buffer ATL?

153895

View

Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?

3761

View

How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite?

3762

View

Can I see error codes on the instrument touchscreen?

3763

View

How are results stored within the QIAcuity Software Suite?

3764

View

What is the scope of a regular maintenance of the QIAcuity?

3765

View

Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex?

3766

View

Can I use an empty nanoplate without seal to perform a dry run?

3767

View

Does an incorrect handling of a plate might affect the results?

3768

View

What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?

3769

View

Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?

3770

View

Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?

3771

View

How can I get the latest VPF (Volume Precision Factor)?

3772

View

Where can I find assays specifically designed for QIAcuity?

3773

View

Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?

3774

View

Can I use the dPCR master mix in a qPCR cycler for optimization purposes?

3775

View

What is the purpose of the reference channel in QIAcuity?

3776

View

How to prepare DNA prior to dPCR?

3778

View

Can I use a custom master mix instead of a QIAGEN master mix?

3777

View

Which sample preparation method is recommended for QIAcuity dPCR?

3779

View

What are the storage conditions and expiry date of QIAcuity consumables?

3780

View

What is the input volume for QIAcuity Nanoplate well?

3782

View

Can I use the QIAcuity Nanoplate in more than one runs?

3781

View

What is the VPF (Volume Precision Factor)?

3784

View

When and how often do I need a new VPF (Volume Precision Factor)?

3785

View

Is a standard curve needed in dPCR?

3786

View

Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?

3788

View

Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine?

3791

View

Is there something to consider when working with transduced cells treated with Polybrene?

3790

View

For sequencing application, does the lysate require purification?

3792

View

Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?

3793

View

Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?

3794

View

Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?

3795

View

What is the minimum cell number needed for cell lysis?

3789

View

For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?

3796

View

What is the sequence of the QIAseq miRNA NGS 5’ Adapter?

FAQ ID - 3672

View

What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits?

3753

View

Are there other options for paraffin removal?

3754

View

Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?

3755

View

Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C?

3756

View

What is the difference between Buffer FTB and Buffer ATL?

3757

View

Can the 2nd Proteinase K step be omitted?

3758

View

What size of DNA can be expected?

3759

View

Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing?

3760

View

Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?

FAQ ID -1056

View

3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?

FAQ ID - 3323

View

Does salt concentration in the food matter when using the DNeasy mericon Food Kit?

FAQ ID - 3346

View

Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?

FAQ ID - 3347

View

When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?

FAQ ID - 3348

View

Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?

FAQ ID - 3349

View

CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?

FAQ ID - 3350

View

What is the composition of QIAzol? What is the color of this reagent?

FAQ ID - 3355

View

Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?

FAQ ID - 3362

View

I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?

FAQ ID - 3363

View

What is the QIAxcel QX RNA Size Marker 200-6000nt?

FAQ ID - 3365

View

What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?

FAQ ID - 3366

View

Do you sell CoralLoad dye separately?

FAQ ID - 3368

View

Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?

FAQ ID - 3369

View

What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?

FAQ ID - 3387

View

Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?

FAQ ID - 3388

View

Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?

FAQ ID - 3389

View

Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?

FAQ ID - 3390

View

Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?

FAQ ID - 3391

View

Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?

FAQ ID - 3392

View

What is the RNase A concentration and composition of Buffer P1?

FAQ ID -198

View

Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?

3797

View

Where can I download the protocol for the KingFisher Flex?

3798

View

Who can help in case of issues with the third party instrument?

3799

View

Is the virus inactivated during the procedure?

3800

View

Can I use sample volumes other than 300 µl?

3801

View

Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?

3802

View

I would like to use the kit on another third party instrument. Who should I contact?

3803

View

How long does a saliva collection take?

3804

View

What can I do to stimulate saliva flow?

3805

View

Can I stop saliva collection before the fill line is reached?

3806

View

What can I do if I don't see the fill line anymore due to saliva bubbles?

3807

View

What happens if I accidentally unscrew the funnel before saliva collection is completed?

3808

View

May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely?

3809

View

What is in the additive in the PAXgene Saliva Collector?

3810

View

What is the shelf life of unused PAXgene Saliva Collectors?

3811

View

How should unused PAXgene Saliva Collector be stored?

3812

View

What should I avoid before sample collection?

3813

View

How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva?

3814

View

How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature?

3815

View

Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C?

3816

View

Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C?

3817

View

Is RNA preserved in saliva collected in PAXgene Saliva Collector?

3818

View

Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?

3819

View

What should I do if stabilized saliva samples are very heterogenous?

3820

View

What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector?

3823

View

Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument?

3824

View

Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation?

3826

View

Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?

3827

View

Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?

3828

View

Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity?

3829

View

Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack?

3825

View

What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector?

3822

View

Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?

3821

View

What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments?

FAQ ID - 3837

View

Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?

FAQ ID - 3836

View

What is the sequence of the UDI 5' Adapter?

FAQ ID - 3835

View

Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit?

FAQ ID - 3674

View

What can be used to validate results obtained with the QIAseq miRNA Library Kit?

FAQ ID - 3675

View

What is the maximum number of available sample indexes?

FAQ ID - 3665

View

What are recommended stopping points during the QIAseq miRNA Library Kit procedure?

FAQ ID - 3663

View

Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?

FAQ ID - 3659

View

What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?

FAQ ID - 3668

View

What is the acceptable number of reads per sample per miRNA sequencing run?

FAQ ID - 3673

View

Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?

FAQ ID -1169

View

Is training of lab personnel included with the purchase of a BioRobot EZ1?

FAQ ID -1239

View

Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

FAQ ID -1240

View

How can we get our BioRobot EZ1 upgraded when new protocols are launched?

FAQ ID -1241

View

Where can I find information about the worktable setup on the BioRobot EZ1?

FAQ ID -1243

View

What disposables are required for nucleic acid isolations on the BioRobot EZ1?

FAQ ID -1246

View

How should I prepare buffy coat samples for use on the BioRobot EZ1?

FAQ ID -1249

View

Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?

FAQ ID -985

View

What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

FAQ ID -528

View

Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?

FAQ ID -975

View

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?

FAQ ID -976

View

Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?

FAQ ID -992

View

Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?

FAQ ID -1025

View

What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?

FAQ ID - 3838

View

What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?

FAQ ID - 3839

View

What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-?

FAQ ID - 3840

View

What PCR enzymes can be used following the first-strand synthesis?

FAQ ID - 3841

View

What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)?

FAQ ID - 3842

View

What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?

2857

View

Does the PyroMark LINE assay target all LINE sequences?

2860

View

What is the amplicon length of the PyroMark CpG LINE assay?

2856

View

How do I perform the PyroMark Q24 Advanced upgrade?

FAQ ID -3163

View

Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?

FAQ ID -3160

View

What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents?

FAQ ID -3165

View

How accurate and reliable is PyroMark Q24 in mutation analysis?

FAQ ID -2094

View

We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions?

FAQ ID - 3621

View

What is the use of the PyroMark Q24 Validation Oligo?

FAQ ID -2855

View

How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?

FAQ ID -2095

View

How long does a single run on the PyroMark Q24 take?

FAQ ID -2096

View

3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram

FAQ ID - 3843

View

When do I have to change the pulse settings/methods in a pyrosequencing run setup?

FAQ ID -2942

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What is a PyroMark instrument method or instrument code?

FAQ ID -2941

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Can the PyroMark Q96 CpG LINE assay be used with an ID system?

2861

View

How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused?

FAQ ID -2863

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How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage.

FAQ ID -2878

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Can unused wells in a pyrosequencing plate be used in the next run?

FAQ ID -2872

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What is the reason for signals ceasing in the middle of a pyrosequencing run?

FAQ ID -2875

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Is the CpG software included in the PyroMark instruments to study methylation status?

FAQ ID -2842

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What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?

FAQ ID -2881

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Where can I find explanations to the warning given by the PyroMark software after run data analysis?

FAQ ID -2874

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Which heating block is recommended for the pyrosequencing annealing step?

FAQ ID -2838

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How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations?

FAQ ID -2848

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What kind of shaker should be used for the pyrosequencing binding step?

FAQ ID -2837

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What is the reason for a high substrate peak in the pyrosequencing pyrogram?

FAQ ID -2879

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How many CpG sites are analyzed by the PyroMark CpG LINE assay?

2858

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Is there a user manual available for the PyroMark Assay design software?

FAQ ID -2851

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Which operating system is compatible with PyroMark IdentiFire Software?

FAQ ID - 3340

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How do I set up a PyroMark CpG Assay?

FAQ ID -2814

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What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?

FAQ ID -2007

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Will the primer sequence for the PyroMark CpG Assay be provided?

FAQ ID -2823

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Does QIAGEN offer a design of Custom PyroMark CpG Assays?

FAQ ID -2818

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How are the PyroMark CpG Assays reconstituted?

FAQ ID -2817

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How are the PyroMark CpG Assays shipped and stored?

FAQ ID -2816

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What are the features of PyroMark CpG Assays, for example, in terms of design and validation?

FAQ ID -2821

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What is included in a PyroMark Custom Assay?

FAQ ID -2815

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In which format can PyroMark CpG Assays be ordered?

FAQ ID -2824

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Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?

FAQ ID -2822

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Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?

FAQ ID -2862

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What is the recommended amplicon size for CpG assays?

FAQ ID -2825

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What kind of reading length can I expect when using pyrosequencing technology for sequence analysis?

FAQ ID -2216

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Will dUTP in a PCR reaction affect pyrosequencing?

FAQ ID -2843

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Which purity grade is recommended for pyrosequencing primers?

FAQ ID -2832

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What concentration should be used for the sequencing primer in pyrosequencing?

FAQ ID -2826

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What is the concentration of PyroMark Control Oligo?

FAQ ID -2846

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What is the sample throughput of pyrosequencing systems?

FAQ ID -2215

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How do I reduce background peaks in the pyrosequencing pyrogram?

FAQ ID -2877

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What is the sensitivity limitation for pyrosequencing?

FAQ ID -2840

View

What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?

FAQ ID -2852

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Which end of the PCR primer for pyrosequencing should be biotinylated?

FAQ ID -2839

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How many nucleotides of a homopolymer can be resolved in pyrosequencing?

FAQ ID -2871

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Where can I order the Streptavidin Sepharose beads for pyrosequencing?

FAQ ID -2850

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What are critical steps during CTC enrichment?

3830

View

How pure are the CTCs enriched from blood samples using the AdnaTest?

3831

View

Can frozen blood be used for the procedure?

3832

View

Can the EZ2 AdnaTest be used for other body fluids than blood?

3833

View

Can Heparin blood be used?

3834

View

Can the kit be used on the EZ2 Connect?

FAQ ID - 3845

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Can I use a different elution buffer?

FAQ ID - 3847

View

What is the average amount of cfDNA present in 1 ml plasma?

FAQ ID - 3846

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Can the kit be used on the EZ1 Advanced XL?

FAQ ID - 3844

View

Do you have a protocol for purification of cytoplasmic RNA from animal cells?

FAQ ID -1257

View

What kits does QIAGEN offer to extract RNA from whole blood?

FAQ ID -304

View

What is the composition of Buffer RLT?

FAQ ID -2793

View

What is the key technical challenge in isolating high quality RNA from cell or tissue samples?

FAQ ID -2656

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Do you have a protocol for the isolation of RNA from leukocytes in milk?

FAQ ID -952

View

What is the maximum binding capacity of RNeasy spin columns?

FAQ ID -290

View

Can QIAquick Kits be used to clean up RNA samples?

FAQ ID -490

View

Are RNeasy spin columns sold separately?

FAQ ID -159

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Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?

FAQ ID -619

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Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?

FAQ ID -467

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How can I minimize degradation of RNA from my pancreas sample?

FAQ ID -624

View

What is the composition of Buffer RW1?

FAQ ID -2796

View

Do you have a protocol for purification of total DNA from crude lysates?

FAQ ID -1255

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Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?

FAQ ID -917

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Do you have a protocol for purification of total DNA from yeast?

FAQ ID -1253

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Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?

FAQ ID -924

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Do you have a protocol for purification of total DNA from insects?

FAQ ID -1254

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How can I precipitate genomic DNA using isopropanol?

FAQ ID -2953

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What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?

FAQ ID - 3447

View

Do you have a protocol for the isolation of genomic DNA from sperm?

FAQ ID -909

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What is the composition of buffer AE?

FAQ ID -730

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How do I safely inactivate biohazardous flow-through material?

FAQ ID -12

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What is the advantage of running an analytical gel with fractions of my plasmid preparation?

FAQ ID -769

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What is the recipe for LB?

FAQ ID -212

View

How do I know if my plasmid is a high- or low copy number type?

FAQ ID -350

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Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?

FAQ ID -366

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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?

FAQ ID -859

View

How do I perform a DNA precipitation to concentrate my sample?

FAQ ID -305

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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?

FAQ ID -864

View

What is the recipe for 2x YT?

FAQ ID -213

View

What are the additional plasmid bands I see on my gel?

FAQ ID -1059

View

Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?

FAQ ID -896

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What is the composition of Buffer PB?

FAQ ID -2791

View

Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?

FAQ ID -3102

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Why is my plasmid DNA yield low?

FAQ ID -768

View

Why do I get genomic DNA contamination in my plasmid prep?

FAQ ID -353

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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?

FAQ ID -898

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I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?

FAQ ID -1045

View

What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?

FAQ ID -861

View

What is the recipe for SOC medium?

FAQ ID -798

View

How much RNA does a typical mammalian cell contain?

FAQ ID -2946

View

Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?

FAQ ID -1037

View

What are the effects of low A260/A230 ratios in RNA preparations on downstream applications?

FAQ ID -2248

View

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

FAQ ID -728

View

Do I need to purchase any consumable for processing the kit on EZ2 Connect?

FAQ ID - 3849

View

Are we able to customize protocol on the EZ2 Connect?

FAQ ID - 3850

View

Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?

FAQ ID - 3851

View

Can the kit be run on EZ1 instruments?

FAQ ID - 3852

View

How can I check the integrity of RNA purified using RNeasy Kits?

FAQ ID -1024

View

What are the limits of detection of the QIAxcel Connect?

FAQ ID - 3853

View

When should the N2 bottle used with the QIAxcel Connect System be changed?

FAQ ID - 3854

View

What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID - 3855

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Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?

FAQ ID - 3856

View

What are the differences between QIAxcel Advanced and QIAxcel Connect?

FAQ ID - 3857

View

Why does an intensity calibration of the gel cartridge for the QIAxcel Connect need to be performed?

FAQ ID - 3858

View

What is the QIAxcel Connect System?

FAQ ID - 3859

View

Can I combine your CGT dPCR assays with custom designed assays?

FAQ ID - 3861

View

How many assays can be multiplexed?

FAQ ID - 3862

View

In which channels can you detect the CGT dPCR assays?

FAQ ID - 3863

View

How can I resuspend the lyophilized assay?

FAQ ID - 3864

View

What quenchers are used?

FAQ ID - 3865

View

Do I need to use restriction enzymes when quantifying AAV genomes?

FAQ ID - 3866

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Can the CGT dPCR assays also be used for the quantification of non-AAV samples?

FAQ ID - 3868

View

Can I use the CGT dPCR assays for the titer determination of RNA viruses?

FAQ ID - 3869

View

How do I know if the assays are compatible with the sample DNA of interest?

FAQ ID - 3870

View

Are the assays also compatible with qPCR?

FAQ ID - 3871

View

Can they be used with lentiviral vectors?

FAQ ID - 3872

View

Is restriction enzyme digestion required?

FAQ ID - 3875

View

dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter?

FAQ ID - 3876

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My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate?

FAQ ID - 3877

View

Is dPCR sensitive to inhibition?

FAQ ID - 3880

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Is DNA extraction from the samples needed?

FAQ ID - 3873

View

How much sample can I load for detecting host cell contamination?

FAQ ID - 3879

View

Where can I find a copy of the .kpf file for a BioSprint protocol?

FAQ ID -3073

View

Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?

FAQ ID -3075

View

What is the minimum number of samples that can be extracted on a BioSprint 96?

FAQ ID -3076

View

Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?

FAQ ID -3077

View

Can I use specific primers with the FastLane Kits?

FAQ ID -3078

View

Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?

FAQ ID -3079

View

Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?

FAQ ID -3080

View

Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?

FAQ ID - 3881

View

Do we have specificity and sensitivity data for this kit?

FAQ ID - 3882

View

Do we have specificity and sensitivity data for this kit?

FAQ ID - 3883

View

Is it possible to scale up sample volume and reaction volume?

FAQ ID - 3884

View

What color channels are used?

FAQ ID - 3885

View

What is the percentage of false negative results that QIAGEN obtained during validations?

FAQ ID - 3886

View

What is the minimum and maximum concentration of effective RNA for the test?

FAQ ID - 3887

View

Anything important when starting the cycler run?

FAQ ID - 3888

View

Is it possible to use ABI instruments without ROX as a passive reference?

FAQ ID - 3889

View

What is the shelf-life of the Neo Assay Kit?

FAQ ID - 3890

View

Can the SARS-CoV-2 Neo Assay Kit detect all variants?

FAQ ID - 3891

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Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?

FAQ ID - 3892

View

Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?

FAQ ID - 3893

View

Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay?

FAQ ID - 3894

View

Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?

FAQ ID - 3895

View

Which positive control should I use?

FAQ ID - 3896

View

How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?

FAQ ID - 3897

View

Which materials can be used as input samples for the QIAsymphony DSP Circulating DNA Kit?

FAQ ID - 3702

View

Are there important considerations for plasma generation and urine handling?

FAQ ID - 3699

View

How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?

FAQ ID - 3898

View

How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?

FAQ ID - 3899

View

Can Phoenix Hot Start Taq utilize cDNA as template for PCR?

FAQ ID - 3900

View

Is Phoenix Hot Start Taq capable of multiplex PCR?

FAQ ID - 3901

View

How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?

FAQ ID - 3902

View

When should I use Phoenix Hot Start Taq GC reaction Buffer?

FAQ ID - 3903

View

What is the amplification length limit of Phoenix Hot Start Taq?

FAQ ID - 3904

View

What is the fidelity/error rate of Phoenix Hot Start Taq?

FAQ ID - 3905

View

Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?

FAQ ID - 3906

View

How can yield for long targets be increased when using Phoenix Hot Start Taq?

FAQ ID - 3907

View

How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?

FAQ ID - 3908

View

How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?

FAQ ID - 3909

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When should I use 5X VeraSeq GC Buffer?

FAQ ID - 3910

View

What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3911

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Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage?

FAQ ID - 3913

View

What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3912

View

How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3914

View

Will VeraSeq 2.0 DNA Polymerase incorporate dUTP?

FAQ ID - 3915

View

Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme?

FAQ ID - 3916

View

How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?

FAQ ID - 3917

View

What denaturation temperature should be used in cycling conditions?

FAQ ID - 3918

View

What annealing temperature should be used in the cycling conditions?

FAQ ID - 3919

View

What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature?

FAQ ID - 3920

View

How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?

FAQ ID - 3922

View

How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?

FAQ ID - 3921

View

When should I use 5X VeraSeq GC Buffer?

FAQ ID - 3923

View

What is the amplification length limit of VeraSeq ULtra DNA Polymerase?

FAQ ID - 3924

View

What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase?

FAQ ID - 3925

View

How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?

FAQ ID - 3926

View

Will VeraSeq ULtra DNA Polymerase incorporate dUTP?

FAQ ID - 3927

View

Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang?

FAQ ID - 3928

View

Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme?

FAQ ID - 3929

View

What denaturation temperature should be used in the cycling conditions?

FAQ ID - 3931

View

What annealing temperature should be used in the cycling conditions?

FAQ ID - 3932

View

What are the ResDNA Quant Kit components?

FAQ ID - 3878

View

How can I load new protocols onto the QIAcube?

FAQ ID -1421

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Are run reports and/or log files available on the QIAcube?

FAQ ID -1412

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What applications are offered on the QIAcube?

FAQ ID -1403

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Can the QIAcube heater/shaker be used independently from protocol runs?

FAQ ID -1422

View

What EZ1 DNA Investigator protocol is recommended for very precious samples?

FAQ ID -1154

View

When should the Normalization protocol be used?

FAQ ID -1155

View

What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?

FAQ ID - 3364

View

How can I extract DNA from a polyacrylamide (PAGE) gel?

FAQ ID -120

View

Why does my DNA sample float out of the slot when loading it onto an agarose gel?

FAQ ID -205

View

What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?

FAQ ID -148

View

I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?

FAQ ID -756

View

Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?

FAQ ID -576

View

Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

FAQ ID -2032

View

Can the QIAcube be connected to a laboratory information management system?

FAQ ID -1411

View

What are the dimensions and weight of the QIAcube?

FAQ ID -1407

View

What dedicated QIAcube Kits are available?

FAQ ID -2337

View

How can I decontaminate the QIAcube system?

FAQ ID -1419

View

Can the QIAcube rotor and/or buckets be removed for cleaning?

FAQ ID -1410

View

Can the QIAcube centrifuge be used independently from protocol runs?

FAQ ID -1423

View

Do I need to buy special kits for the QIAcube?

FAQ ID -1402

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Do I need to discard partially used QIAcube tip racks?

FAQ ID -1418

View

Can I program my own protocols for the QIAcube?

FAQ ID -1405

View

Can the CGT dPCR assays also be used for the analysis of AAV genome integrity?

FAQ ID - 3867

View

Is the QIAprep&amp CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN?

3787

View

Do you have a protocol for the isolation of viral RNA from stool?

FAQ ID -918

View

Which extraction kits are recommended?

FAQ ID - 3874

View

How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C?

FAQ ID - 3934

View

What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)?

FAQ ID - 3935

View

What is the amount of standard for the different kit?

FAQ ID - 3936

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How did you calculate the conversion factor?

FAQ ID - 3937

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What does the standard kit contain?

FAQ ID - 3938

View

What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)?

FAQ ID - 3939

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How long are sample lysates stable in the fridge/ freezer?

FAQ ID - 143765

View

Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

FAQ ID -2027

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Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?

FAQ ID -2031

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After bisulfite conversion, can the DNA be stored?

FAQ ID -2409

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Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?

FAQ ID - 3940

View

When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?

FAQ ID - 3941

View

What size of RNA can be expected?

FAQ ID - 3942

View

Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?

FAQ ID - 3947

View

When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit

FAQ ID - 3948

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Are there other options for paraffin removal?

FAQ ID - 3949

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FAQ ID - 3950

View

Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)?

FAQ ID - 3951

View

An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away.

FAQ ID - 3952

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Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue?

FAQ ID - 3953

View

What size of RNA can be expected?

FAQ ID - 3954

View

Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID - 3955

View

My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?

FAQ ID - 3956

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Why are my DV200 values of my RNA sample so low?

FAQ ID - 3957

View

The integrity of my DNA sample (e.g. QIAxcel Connect) is bad.

FAQ ID - 3958

View

How do I proceed if the amount of starting material cannot be calculated in detail?

FAQ ID - 3943

View

Are there other options for paraffin removal?

FAQ ID - 3946

View

Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID - 3944

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My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?

FAQ ID - 3945

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Is this available without glycerol?

FAQ ID - 3959

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What types of priming are compatible with EnzScript M-MLV RT RNase H-?

FAQ ID - 3961

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What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)?

FAQ ID - 3964

View

What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?

FAQ ID - 3960

View

What PCR enzymes can be used following first-strand synthesis?

FAQ ID - 3963

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Is the kit compatible with unpurified in-process samples?

FAQ ID - 3965

View

Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration?

FAQ ID - 3971

View

Do you recommend storage of highly diluted AAV samples?

FAQ ID - 3972

View

Is it possible to store the lysates? How can the lysates be stored?

FAQ ID - 3973

View

Can I use MspI or HpaII restriction enzymes from other suppliers?

FAQ ID - 3975

View

How many AAV genome copies can I load into the QIAcuity?

FAQ ID - 3977

View

Do you need to dilute the lysates before setting up the PCR mix?

FAQ ID - 3978

View

How do I know if the CGT dPCR assays are compatible with the sample DNA of interest?

FAQ ID - 3980

View

Are the CGT dPCR assays also compatible with qPCR?

FAQ ID - 3981

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Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays?

FAQ ID - 3982

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What is included in the SureSilencing shRNA Plasmids shipment?

FAQ ID -2785

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Is the QIAxcel Advanced discontinued?

FAQ ID - 3983

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What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit?

FAQ ID - 3984

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What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?

FAQ ID - 3985

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What water should I use to prepare the buffer concentrates?

FAQ ID - 3986

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What is the new Waste Tube made of?

FAQ ID - 3987

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Are the QIAwave kits based on a different chemistry than the legacy kits?

FAQ ID - 3990

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Is there a way to compare the environmental impacts of the kit?

FAQ ID - 3991

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Can the QIAwave kits be recycled?

FAQ ID - 3992

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Does the reuse of the Waste Tubes increase the risk of cross-contamination?

FAQ ID - 3988

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I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?

FAQ ID - 3989

View

Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument?

FAQ ID - 3993

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Is the kit compatible with unpurified in-process samples?

FAQ ID - 3994

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My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction?

FAQ ID - 3995

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Can I use Internal Control as overall control?

FAQ ID - 3996

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How long can I store the reconstituted Internal Control and the reconstituted Positive Control?

FAQ ID - 3997

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Do you recommend storage of highly diluted mycoplasma samples?

FAQ ID - 3998

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What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit?

FAQ ID - 3999

View

On which instrumentation will the RT² Profiler PCR Array work?

FAQ ID -2719

View

Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?

FAQ ID -2569

View

Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?

FAQ ID -2571

View

Which human target is used for the quantification in the Investigator Quantiplex Kit?

FAQ ID -2577

View

Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?

FAQ ID - 3728

View

Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3?

FAQ ID -2570

View

Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit?

FAQ ID - 3730

View

Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit?

FAQ ID - 3743

View

Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit?

FAQ ID - 3742

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Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System?

FAQ ID - 3732

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How long does a run take?

FAQ ID -2566

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Investigator Quantiplex: For which real-time cyclers has the kit been validated?

FAQ ID -2567

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What are the storage conditions?

FAQ ID - 3748

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Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?

FAQ ID -2568

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How can I streamline the quantification workflow?

FAQ ID - 3738

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Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q?

FAQ ID - 3729

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Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated?

FAQ ID - 3741

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Can I optimize a dPCR assay on the QIAcuity using gradient temperature?

FAQ ID - 3783

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?

FAQ ID -2126

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Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2130

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2131

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What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

FAQ ID -761

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Can I use REPLI-g for SNP Genotyping?

FAQ ID -700

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Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?

FAQ ID -2058

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What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?

FAQ ID - 3506

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Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?

FAQ ID -2595

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Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?

FAQ ID -717

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Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?

FAQ ID -1979

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?

FAQ ID -1452

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How is "Touchdown PCR" used to increase PCR specificity?

FAQ ID -75

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What should the starting template DNA quality and quantity be for PCR?

FAQ ID -74

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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?

FAQ ID -165

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Why do I get smeared PCR products?

FAQ ID -87

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Does QIAGEN sell Q-Solution separately?

FAQ ID -204

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Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?

FAQ ID -1745

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Is Q-Solution required for PCR with QIAGEN's PCR kits?

FAQ ID -380

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Have you tested the effect of inhibitors on PCR performance?

FAQ ID -818

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What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?

FAQ ID -606

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Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility?

FAQ ID - 3974

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What is the recommended HpaII concentration?

FAQ ID - 3976

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Can I use the lysate from the CGT Viral Vector Lysis kit and combine it with another PCR system?

FAQ ID - 3979

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Do you have any recommendations on how to process my AAV samples?

FAQ ID - 3860

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