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QPCR

 

Q-Rex: PCR software that does it all

The Q-Rex operating and analysis software provides unique features to facilitate fast analysis of your experiments on the QIAquant and Rotor-Gene Q real-time PCR instruments.

It enables experiment setup and data acquisition and performs basic analyses. In addition, you can expand Q-Rex functionality with plug-ins for specific capabilities such as absolute quantification and genotype analysis.

The software is suitable for use by operators new to real-time PCR while maintaining the capacity for highly complex data analysis required for advanced research.

Get the most out of your real-time PCR data with Q-Rex Software and QIAquant for simple experiment setup and fast analysis of your real-time PCR.
  • See how Q-Rex helps you normalize your data
    Normalization is the first step for analysis of your qPCR data. During normalization, background noise is subtracted to clear up the real signal.
  • Q-Rex can help you to set the optimal threshold
    Cq values are among the most important data for qPCR analysis. Cq values are defined by the threshold, so interpretation of your results relies on correctly setting this significant analysis parameter.
  • Look at raw data side-by-side with normalized data
    Raw data of a qPCR experiment show the unfiltered, true signals. Q-Rex lets you look at raw data side-by side with normalized data to help you troubleshoot when results don’t look as expected.
  • Simplifying gene expression analysis with Q-Rex
    With gene expression, changes in expression of a specific gene of interest can be evaluated. The delta delta Cq method is the most commonly used analysis method for calculation of the fold change in gene expression.
  • Plug-ins expand Q-Rex software for your specific analysis needs
    Q-Rex core software supports you through basic experiment setup and data acquisition. For specific analytical requirements, you can easily expand the capacity of Q-Rex software with plug‑ins. Q‑Rex plug-ins allow you to create the exact software configuration you need without installing functions you will never use.
  • Video thumbnail
    See how Q-Rex helps you normalize your data
  • Video thumbnail
    Q-Rex can help you to set the optimal threshold
  • Video thumbnail
    Look at raw data side-by-side with normalized data
  • Video thumbnail
    Simplifying gene expression analysis with Q-Rex
  • Plug-ins expand Q-Rex software for your specific analysis needs
The core software of Q-Rex enables experiment setup and data acquisition, while the plug-ins expand the capacity of Q-Rex with specific analytical capabilities. It allows you to easily create the exact software configuration to meet your specific analysis needs without installing functions you will never use. Let’s take a closer look at the plug-ins and what they can do:
Q-Rex Basic Plug-in
  • Suitable for instrument users getting started with general qPCR and RT-PCR
  • Drives the hardware, sets up qPCR, melt analyses and multiplexing experiments
  • Collects raw data and performs basic Cq analysis

Q-Rex Gene Expression Advanced Plug-in
  • Analyzes changes in gene expression relative to a reference sample
  • Allows multiple reference gene normalization based on algorithms recommended in industry-leading literature 
  • Defines a stable reference gene with the help of plots 
  • Allows normalization of values in relation to a defined calibrator sample
  • Defines the real qPCR efficiency for accurate results 

Q-Rex Absolute Quantification Plug-in
  • Calculates the absolute concentration of targets based on a standard curve
  • Applications include:
    • Quantification of NGS libraries 
    • Quantification for exosome enrichment 
    • Quantification for assessing genome structure like telomeres

Q-Rex End Point Analysis Plug-in
  • Can be used for probe-based SNP genotyping 
  • Automates genotype calling based on advanced algorithms for easy and convenient genotype analysis
  • Delivers both raw and normalized data analysis

Q-Rex Melt Curve Analysis Plug-in
  • Performs advanced analysis that can be applied for genotyping or multiplex pathogen detection of >15 targets
  • New data processing algorithms for easy and improved melt peak detection
  • High multiplex performance comes from use of TaqMelt™ technology and powerful algorithms that improve analysis and enable combination potential

Q-Rex Target Detection Plug-in
  • Versatile; detects the presence or absence of targets based on user-defined criteria 
  • Delivers results as an intuitive stoplight visualization 
  • For the Rotor-Gene Q 
    • Includes pre-defined assay rules for several QIAGEN Assays
    • Accommodates multiplexing with FRET probes
  • For the QIAquant
    • Users can design lab-developed assays with adapted rules

Q-Rex Absolute Quantification Plug-in HID (only for RGQ)
  • Calculates the absolute concentration of targets in experimental samples using the amplification curves of samples of known concentration to establish a standard
  • Has an automatic threshold function based on the defined standards that scans through all possible threshold levels until the best fit is found for the standard curve

Q-Rex High Resolution Melt Plug-in (only for RGQ)
  • Simplifies high-resolution melting analysis to detect small sequence differences 
  • Performs automated genotype calling based on standards
  • Calculates confidence levels for all results
  • Results are reported on difference plots for meaningful and clear visualization

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