质保、质控和安全数据 - FAQ



常见问题打印 Bookmark 分享 more.. 您无权限查看此资源分类选项按字母顺序排序按相关性排列 What are exo-resistant random hexamers used in the REPLI-g reaction? FAQ ID -710 浏览 The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem? FAQ ID -776 浏览 Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit? FAQ ID -797 浏览 What is the basic technology behind the Strep-tag Protein Purification System? FAQ ID -740 浏览 What are the expected average DNA yields from plant extractions on the BioSprint 96? FAQ ID -771 浏览 If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits? FAQ ID -718 浏览 How to quantitate DNA purified using the BioSprint 96? FAQ ID -773 浏览 How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead? FAQ ID -734 浏览 Is it possible to use LiquiChip beads for FACS applications? FAQ ID -733 浏览 Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately? FAQ ID -763 浏览 Is GFP excited and detected by the green laser of the LiquiChip System? FAQ ID -737 浏览 Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples? FAQ ID -755 浏览 Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not? FAQ ID -758 浏览 Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers? FAQ ID -715 浏览 Why should DNA or cDNA targets be less than 250 bp long for real-time PCR? FAQ ID-751 浏览 Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits? FAQ ID -719 浏览 Why might my gene of interest drop out during WGA if it is not near a telomere or centromere? FAQ ID -703 浏览 Will the random hexamers in the REPLI-g reaction interfere with downstream analysis? FAQ ID -713 浏览 Why is there no detection of free reporter fluorescence in the LiquiChip System? FAQ ID -732 浏览 What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR? FAQ ID -782 浏览 What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit? FAQ ID -779 浏览 What is the enzyme used in the REPLI-g reaction? FAQ ID -704 浏览 What type of Sequencing Services does QIAGEN offer? FAQ ID -781 浏览 Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604? FAQ ID -766 浏览 What is the general sensitivity level of the LiquiChip System? FAQ ID -731 浏览 Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing? FAQ ID -741 浏览 Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size? FAQ ID -778 浏览 Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen? FAQ ID -757 浏览 Can lyophilized plant material be used for DNA extraction on the BioSprint 96? FAQ ID -772 浏览 Can the REPLI-g WGA enzyme work with labeled dNTPs? FAQ ID -706 浏览 Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately? FAQ ID -744 浏览 Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates? FAQ ID -722 浏览 Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately? FAQ ID -791 浏览 Any data on the fidelity of the REPLI-g MDA technique? FAQ ID -707 浏览 Are Centromeres and Telomeres amplified using REPLI-g WGA? FAQ ID -702 浏览 Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region? FAQ ID -712 浏览 Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit? FAQ ID -716 浏览 How can one perform multiplex real-time PCR analysis using the LightCycler 2.0? FAQ ID -780 浏览 How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression? FAQ ID -788 浏览 How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination? FAQ ID -783 浏览 How many cells can be used with the FastLane Cell cDNA Kit? FAQ ID -796 浏览 Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA? FAQ ID -746 浏览 Can frozen blood be used for whole genome amplification with REPLI-g? FAQ ID -794 浏览 Do I have to switch off the laser of the LiquiChip Workstation overnight? FAQ ID -736 浏览 How many reporter dyes can be used in the same analysis using the LiquiChip Workstation? FAQ ID -738 浏览 Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits? FAQ ID -714 浏览 Do you synthesize thiophosphate modified random hexamers? FAQ ID -711 浏览 Has anyone verified whole genome amplification accuracy with Sequencing? FAQ ID -701 浏览 Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA? FAQ ID -793 浏览 How best to handle the light sensitive LiquiChip reagents? FAQ ID -735 浏览 Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR? FAQ ID -729 浏览 Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2? FAQ ID -397 浏览 Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit? FAQ ID -358 浏览 Is it possible to store TAGzyme enzymes at°C? -80 浏览 I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do? FAQ ID -364 浏览 What are the calculated molecular weights of the TAGZyme enzymes? FAQ ID -329 浏览 How complete is the removal of DAPase in the TAGZyme process? FAQ ID -319 浏览 How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs? FAQ ID -392 浏览 How do I submit a siRNA order by telephone or online? FAQ ID -399 浏览 How many transfections can I perform with 20 nmol of HPP grade siRNA? FAQ ID -367 浏览 Which primers can I use for sequencing pQE-expression vector constructs? FAQ ID -343 浏览 What is the standard assay volume recommended for LiquiChip assays? FAQ ID -330 浏览 What is the acceptable maximum g force for centrifugation of Xa Removal Resin? FAQ ID -320 浏览 What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules? FAQ ID -307 浏览 What is the composition of Buffer FG3? FAQ ID -356 浏览 Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits? FAQ ID -354 浏览 303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? 浏览 Can I use a partial MinElute 96 UF plate? FAQ ID -355 浏览 Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells? FAQ ID -372 浏览 Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits? FAQ ID -306 浏览 Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable? FAQ ID -308 浏览 Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein? FAQ ID -359 浏览 Are QIAGEN monoclonal Anti His Antibodies glycosylated? FAQ ID -334 浏览 Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit? FAQ ID -332 浏览 Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR? FAQ ID -328 浏览 Do I need to anneal, deprotect or desalt my QIAGEN siRNA? FAQ ID -398 浏览 How are siRNAs introduced into C. elegans? FAQ ID -396 浏览 Do pQE vectors contain the CAT gene? FAQ ID -362 浏览 How can I improve the expression of proteins containing hydrophobic regions? FAQ ID -339 浏览 How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution? FAQ ID -324 浏览 Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector? FAQ ID -323 浏览 Can RNAprotect Tissue Reagent be used to store previously isolated RNA? FAQ ID -337 浏览 Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil? FAQ ID -360 浏览 Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining? FAQ ID -312 浏览 Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton? FAQ ID -327 浏览 Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex? FAQ ID -318 浏览 What data documentation does QIAGEN's Sequencing Service provide for customers? FAQ ID -832 浏览 What are the sequences of the FlexiTube siRNAs? FAQ ID -851 浏览 My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols? FAQ ID -872 浏览 What is the proper way to turn on or turn off the EZ1 instrument? FAQ ID -870 浏览 What is the Energy Mix in the EasyXpress Kits? FAQ ID -846 浏览 What is the concentration of the primers in a reaction using the QuantiTect Primer Assays? FAQ ID -850 浏览 I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA? FAQ ID -829 浏览 Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit? FAQ ID -835 浏览 Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate? FAQ ID -854 浏览 Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit? FAQ ID -855 浏览 Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit? FAQ ID -841 浏览 Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit? FAQ ID -876 浏览 Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit? FAQ ID -837 浏览 Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity? FAQ ID -875 浏览 Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit? FAQ ID -878 浏览 Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation? FAQ ID -862 浏览 Will acetone precipitation recommended in the Qproteome Protocols denature Protein? FAQ ID -807 浏览 Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein? FAQ ID -877 浏览 Will the sequences of the QuantiTect Primer Assays be provided? FAQ ID -804 浏览 Will the FastLane Cell cDNA Kit work with suspension cells? FAQ ID -801 浏览 Which resin is used in the QIAexpress Ni-NTA Fast Start Columns? FAQ ID -836 浏览 What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in? FAQ ID -815 浏览 What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit? FAQ ID -873 浏览 What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment? FAQ ID -831 浏览 Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx? FAQ ID -806 浏览 Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit? FAQ ID -839 浏览 Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit? FAQ ID -808 浏览 Where can I find QIAGEN products for a specific gene or gene product? FAQ ID -803 浏览 Can the QuantiTect Primer Assays be used for detection of genomic DNA? FAQ ID -811 浏览 Do I need to use RNase inhibitors with the RNeasy Kits? FAQ ID -813 浏览 Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent? FAQ ID -824 浏览 Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR? FAQ ID -834 浏览 Can the FastLane Cell cDNA Kit be used with tissue? FAQ ID -821 浏览 Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits? FAQ ID -883 浏览 Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit? FAQ ID -881 浏览 895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit? 浏览 Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4? -15 浏览 Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1? FAQ ID -823 浏览 Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID -828 浏览 Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation? FAQ ID -856 浏览 Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast? FAQ ID -838 浏览 Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits? FAQ ID -874 浏览 Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit? FAQ ID -812 浏览 Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems? FAQ ID -863 浏览 Do you have information on in vivo RNA interference experiments? FAQ ID -817 浏览 Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately? FAQ ID -825 浏览 I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast? FAQ ID -822 浏览 How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits? FAQ ID -880 浏览 How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit? FAQ ID -843 浏览 Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.? FAQ ID -893 浏览 Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii? FAQ ID -885 浏览 Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum? FAQ ID -886 浏览 Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.? FAQ ID -884 浏览 Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.? FAQ ID -887 浏览 Do you have a protocol for the isolation of plasmid DNA from yeast? FAQ ID -853 浏览 Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits? FAQ ID -894 浏览 Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.? FAQ ID -890 浏览 Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans? FAQ ID -888 浏览 Do you have a protocol for the isolation of plasmid DNA from Proteus spp.? FAQ ID -889 浏览 What are your recommendations for cotransfecting several plasmids? FAQ ID -124 浏览 What are the recommended culture and buffer volumes for a very low-copy plasmid? FAQ ID -168 浏览 Is mRNA isolation necessary for sensitive RT-PCR? FAQ ID -111 浏览 When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit? FAQ ID -100 浏览 Which Anti-His Antibody is the most sensitive for my protein of interest? FAQ ID -172 浏览 Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101 浏览 What is the recommended culture medium for the QIAprep System? FAQ ID -154 浏览 How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification? FAQ ID -102 浏览 How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology? FAQ ID -134 浏览 How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit? FAQ ID -141 浏览 How can I isolate RNA from 1 gram of plant tissue? FAQ ID -128 浏览 How can I improve the performance of the HiSpeed QIAprecipitator module? FAQ ID -144 浏览 What can I use to isolate RNA smaller than 200 nucleotides? FAQ ID -115 浏览 What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize? FAQ ID -170 浏览 What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit? FAQ ID -167 浏览 How should RNeasy Kits be stored and how long are they stable? FAQ ID -103 浏览 Is it possible to cleave the 6xHis-tag from an expressed protein? FAQ ID -140 浏览 How should QIAGEN Plasmid Purification Kits be stored and for how long? FAQ ID -192 浏览 What are the features and benefits of the QIAexpress 6xHis Tag System? FAQ ID -193 浏览 Do you have transfection data for QIAGEN Transfection Reagents? FAQ ID -158 浏览 Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit? FAQ ID -129 浏览 Does QIAGEN offer vectors for direct cloning of PCR products? FAQ ID -146 浏览 How can I avoid variable transfection efficiencies with SuperFect Reagent? FAQ ID -179 浏览 Can DyeEx be used to clean up labeled DNA other than sequencing templates? FAQ ID -137 浏览 Are QIAamp DNA isolation kits suitable for apoptosis studies? FAQ ID -149 浏览 Can I use a QIAfilter Cartridge for purifying large plasmids? FAQ ID -121 浏览 Can I use Omniscript or Sensiscript RT's at a higher temperature? FAQ ID -116 浏览 How do you ensure that RNeasy buffers are RNase-free? FAQ ID -113 浏览 How can I purify DNA from soil, food and sewage samples for PCR? FAQ ID -118 浏览 How should I store Anti·His and Tag·100 Antibodies? FAQ ID -194 浏览 How do I prepare an insert for pQE vectors? FAQ ID -185 浏览 How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit? FAQ ID -143 浏览 What are the compatibilities of different reagents with Ni-NTA matrices? FAQ ID -49 浏览 How can I prevent running out of M48 Kit reagents before the full number of samples are processed? FAQ ID -1042 浏览 Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48? FAQ ID -1019 浏览 Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation? FAQ ID -1040 浏览 Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits? FAQ ID -1071 浏览 Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA? FAQ ID -1063 浏览 Is it possible to use the PhosphoProtein Purification Kit in batch format? FAQ ID -1069 浏览 What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components? FAQ ID -1077 浏览 What is the difference between TurboCapture Microplate Holders Type A and Type B? FAQ ID -1038 浏览 What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies? FAQ ID -1093 浏览 Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit? FAQ ID -1049 浏览 How can I transfect siRNA into insect cells such as Drosophila melanogaster S2? FAQ ID -1046 浏览 How can I tell TurboCapture Microplate Holder Type A from Type B? FAQ ID -1039 浏览 How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits? FAQ ID -1086 浏览 How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent? FAQ ID -1058 浏览 What is the error rate of HotStar HiFidelity DNA Polymerase? FAQ ID -1048 浏览 Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells? FAQ ID -1092 浏览 Which kit should I use for RNA isolation from Cartilage? FAQ ID -1026 浏览 Where can I find the serial number on QIAGEN's BioRobots? FAQ ID -1064 浏览 Which kit can be used for simultaneous purification of DNA and RNA from biopsies? FAQ ID -1089 浏览 Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column? FAQ ID -1044 浏览 Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature? FAQ ID -1083 浏览 Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit? FAQ ID -1054 浏览 Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase? FAQ ID -1053 浏览 Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits? FAQ ID -1084 浏览 What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve? FAQ ID -1079 浏览 What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit? FAQ ID -1090 浏览 What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)? FAQ ID -1055 浏览 What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits? FAQ ID -1034 浏览 How can I prevent clogging of QIAfilter cartridges? FAQ ID -1060 浏览 What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit? FAQ ID -1067 浏览 Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays? FAQ ID -1065 浏览 Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase? FAQ ID -1050 浏览 Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent? FAQ ID -1020 浏览 Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96? FAQ ID -1007 浏览 Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48? FAQ ID -1017 浏览 Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument? FAQ ID -1032 浏览 Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene? FAQ ID -1091 浏览 Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit? FAQ ID -1078 浏览 Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells? FAQ ID -1036 浏览 Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably? FAQ ID -1066 浏览 Can the AllPrep RNA/Protein Kit be used with tissue? FAQ ID -1095 浏览 Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies? FAQ ID -1073 浏览 How can air bubbles in PhosphoProtein Purification Columns be avoided? FAQ ID -1070 浏览 How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits? FAQ ID -1062 浏览 How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set? FAQ ID -1087 浏览 Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot? FAQ ID - 1004 浏览 Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks? FAQ ID -1014 浏览 Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent? FAQ ID -1013 浏览 Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect? FAQ ID -1012 浏览 Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot? FAQ ID -1009 浏览 Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits? FAQ ID -1011 浏览 Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot? FAQ ID -1008 浏览 Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot? FAQ ID -1010 浏览 Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot? FAQ ID -1006 浏览 Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot? FAQ ID - 1003 浏览 Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles? FAQ ID -2471 浏览 If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have? FAQ ID -266 浏览 Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA? FAQ ID -297 浏览 What are the main differences between other magnetic beads and the BioMag particles? FAQ ID -272 浏览 My BioMag is about to expire, is it still any good? FAQ ID -268 浏览 How magnetically responsive is BioMag? FAQ ID -265 浏览 If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos? FAQ ID -279 浏览 Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol. FAQ ID -283 浏览 What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit? FAQ ID -292 浏览 What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit? FAQ ID -295 浏览 What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific? FAQ ID -275 浏览 Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit? FAQ ID -287 浏览 Can I select FITC labeled cells with BioMag? FAQ ID -278 浏览 Can I use BioMag particles with any specific primary antibody? FAQ ID -273 浏览 Are BioMag particles stable in other solvents? FAQ ID -267 浏览 Can BioMag be used for positive and negative selection? FAQ ID -269 浏览 How can I avoid poor immunolocalization morphology with Anti-His Antibodies? FAQ ID -200 浏览 How can I prevent non-specific binding when using BioMag? FAQ ID -277 浏览 How do I prevent bubbles from forming in my Ni-NTA agarose column? FAQ ID -285 浏览 How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit? FAQ ID -201 浏览 Is your Nuclease-Free Water fluorescence-free? FAQ ID -1291 浏览 What quality level does your Nuclease-Free Water have? FAQ ID -1292 浏览 How should the GelPilot DNA Loading Dye be handled? FAQ ID -1287 浏览 Does GelPilot DNA Loading Dye contain ethidium bromide? FAQ ID -1286 浏览 Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time? FAQ ID -1299 浏览 Are MaXtract tubes siliconized? FAQ ID -1298 浏览 Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples? FAQ ID -1211 浏览 What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1205 浏览 Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit? FAQ ID -1213 浏览 Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1? FAQ ID -1232 浏览 Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately? FAQ ID -1217 浏览 Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II? FAQ ID -1225 浏览 What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II? FAQ ID -1224 浏览 What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II? FAQ ID -1223 浏览 What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ? FAQ ID -1208 浏览 Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells? FAQ ID -1202 浏览 What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose? FAQ ID -1206 浏览 What is the protein yield when using the Qproteome Plasma Membrane Protein Kit? FAQ ID -1201 浏览 What is the size of the BioRobot EZ1? FAQ ID -1245 浏览 Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II? FAQ ID -1226 浏览 Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit? FAQ ID -1207 浏览 Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit? FAQ ID -1258 浏览 Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)? FAQ ID -1216 浏览 Can glycosylated proteins be expressed using the EasyXpress Insect Kit II? FAQ ID -1227 浏览 Can RNAprotect Saliva Reagent be purchased separately? FAQ ID -1214 浏览 Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells? FAQ ID -1215 浏览 Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused? FAQ ID -1204 浏览 Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples? FAQ ID -1200 浏览 Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen? FAQ ID -1248 浏览 How can I decontaminate the BioRobot EZ1? FAQ ID -1231 浏览 How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit? FAQ ID -1212 浏览 How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take? FAQ ID -1222 浏览 How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate? FAQ ID -1219 浏览 Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1? FAQ ID -1260 浏览 Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA? -1 浏览 How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure? FAQ ID -1203 浏览 Which fixation method is used in the PAXgene Tissue System? FAQ ID - 3600 浏览 What is the fixation reagent of PAXgene Tissue FIX? FAQ ID - 3601 浏览 What is the stabilization reagent of PAXgene Tissue STABILIZER? FAQ ID - 3602 浏览 What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)? FAQ ID - 3604 浏览 Why are two reagents used in the PAXgene Tissue System? FAQ ID - 3603 浏览 Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER? FAQ ID - 3605 浏览 Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run? FAQ ID - 3606 浏览 Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue? FAQ ID - 3607 浏览 Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing? FAQ ID - 3608 浏览 Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues? FAQ ID - 3609 浏览 How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3611 浏览 Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3610 浏览 How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3612 浏览 Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue? FAQ ID - 3614 浏览 What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue? FAQ ID - 3613 浏览 Can proteins be extracted from PAXgene Tissue fixed specimens? FAQ ID - 3616 浏览 Does the PAXgene Tissue RNA Kit co-purify small RNAs? FAQ ID - 3615 浏览 Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens? FAQ ID - 3617 浏览 What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID - 3618 浏览 How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue? FAQ ID - 3619 浏览 Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit? FAQ ID - 3623 浏览 How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)? FAQ ID - 3622 浏览 Are QR codes recognized by QIAsymphony? FAQ ID - 3625 浏览 Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube? FAQ ID - 3626 浏览 What is the reagent in the PAXgene Blood ccfDNA Tube? FAQ ID - 3632 浏览 Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence? FAQ ID - 3627 浏览 What is the shelf life of unused PAXgene Blood ccfDNA Tubes? FAQ ID - 3633 浏览 How should unused PAXgene Blood ccfDNA Tubes be stored? FAQ ID - 3634 浏览 Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)? FAQ ID - 3635 浏览 Are PAXgene Blood ccfDNA Tubes sterile? FAQ ID - 3636 浏览 What is the expected ccfDNA yield from plasma generated from 10 ml of blood? FAQ ID - 3637 浏览 Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes? FAQ ID - 3638 浏览 How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature? FAQ ID - 3639 浏览 Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C? FAQ ID - 3640 浏览 Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C? FAQ ID - 3641 浏览 Can whole blood be frozen in PAXgene Blood ccfDNA Tubes? FAQ ID - 3642 浏览 Is double centrifugation required for plasma processing? FAQ ID - 3643 浏览 How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored? FAQ ID - 3644 浏览 What should I do if cryoprecipitates form while thawing a frozen plasma sample? FAQ ID - 3646 浏览 Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols? FAQ ID - 3648 浏览 How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols? FAQ ID - 3649 浏览 How long after first use can a reagent cartridge be stored for further use? FAQ ID - 3650 浏览 For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended? FAQ ID - 3652 浏览 Which tubes are recommended for the QIAsymphony sample rack FAQ ID - 3653 浏览 Which protocol line should I use to process plasma samples? FAQ ID - 3654 浏览 Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl? FAQ ID - 3656 浏览 How should eluates produced with the PAXgene Blood ccfDNA System be stored? FAQ ID - 3657 浏览 Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System? FAQ ID - 3658 浏览 What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit? FAQ ID - 3651 浏览 Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit? FAQ ID - 3655 浏览 Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube? FAQ ID - 3628 浏览 Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm? FAQ ID - 3629 浏览 Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube? FAQ ID - 3630 浏览 What is the blood draw volume of the PAXgene Blood ccfDNA Tube FAQ ID - 3631 浏览 What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version? FAQ ID - 3685 浏览 Where can I find the gene list for a specific RT² Profiler PCR Array? FAQ ID - 3684 浏览 Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit? FAQ ID - 3680 浏览 Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit? FAQ ID - 3660 浏览 Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples? FAQ ID - 3661 浏览 Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits? FAQ ID - 3662 浏览 Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library? FAQ ID - 3666 浏览 What method is used to eliminate adapter dimers from the QIAseq miRNA library? FAQ ID - 3667 浏览 Does a QIAseq miRNA library include hY4 Y RNA? FAQ ID - 3670 浏览 What is the sequence of the QIAseq miRNA NGS 3’ Adapter? FAQ ID - 3671 浏览 Do the covered regions in the BED file include the primers? FAQ ID - 3689 浏览 What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box? FAQ ID - 3697 浏览 Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA? FAQ ID - 3637 浏览 Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System? FAQ ID -1191 浏览 Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide? FAQ ID -1107 浏览 Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH? FAQ ID -1123 浏览 What are the applications the EasyXtal 15-Well Tool can be used for? FAQ ID -1111 浏览 Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots? FAQ ID -1118 浏览 Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol? FAQ ID -1187 浏览 What cell lines have been tested with the Fastlane Cell cDNA Kit? FAQ ID -1175 浏览 What does 'QIAGEN Certified Solution' for Protein Crystallization mean? FAQ ID -1121 浏览 How many protein crystallization conditions are available at QIAGEN? FAQ ID -1119 浏览 How much detection reagent do you generally recommend for LiquiChip Assays? FAQ ID -1159 浏览 How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected? FAQ ID -1189 浏览 How many drops can be set up on a protein crystallization support? FAQ ID -1110 浏览 How many LiquiChip beads are recommended for an xMAP experiment? FAQ ID -1161 浏览 How much solution can I add to an EasyXtal 15-Well Tool reservoir well? FAQ ID -1114 浏览 In which formats are crystallization condition Screening Suites available? FAQ ID -1127 浏览 If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human? FAQ ID -1145 浏览 If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work? FAQ ID -1136 浏览 How sensitive is the LiquiChip Reader? FAQ ID -1157 浏览 How much time is needed for a LiquiChip assay point measurement? FAQ ID -1162 浏览 Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)? FAQ ID -1133 浏览 What is the delivery time for QuantiTect Primer Assays? FAQ ID -1142 浏览 Where can I find the composition tables for solutions used in individual Crystallization Screening Suites? FAQ ID -1124 浏览 What kind of strategy for initial screening of protein crystallization conditions do you recommend? FAQ ID -1120 浏览 When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat? FAQ ID -1139 浏览 What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol? FAQ ID -1193 浏览 What starting material can be used with the Qproteome Mitochondria Isolation Kit? FAQ ID -1184 浏览 Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube? FAQ ID -1153 浏览 Are EasyXtal 15-Well Tools stackable? FAQ ID -1116 浏览 Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN? FAQ ID -1138 浏览 Which QuantiTect Primer Assay should I choose for my gene of interest? FAQ ID -1135 浏览 Why does the QuantiTect Primer Assay for my gene of interest have only one version number? FAQ ID -1134 浏览 Which chemical products do you use to prepare screening solutions for protein crystallization? FAQ ID -1122 浏览 Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand? FAQ ID -1195 浏览 Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively? FAQ ID -1198 浏览 Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1199 浏览 Why is there no QuantiTect Primer Assay for my gene of interest? FAQ ID -1140 浏览 What is the EasyXtal 15-Well Tool made of? FAQ ID -1106 浏览 What is the dynamic range of detection of the LiquiChip Reader? FAQ ID -1158 浏览 Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation? FAQ ID -1185 浏览 Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit? FAQ ID -1151 浏览 Can the EasyXtal 15-Well Tools be reused? FAQ ID -1117 浏览 Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells? FAQ ID -1194 浏览 Can your Tag antibody recognize rat MAPK1 also? -100 浏览 Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN? FAQ ID -1156 浏览 Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays? FAQ ID -1197 浏览 Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused? FAQ ID -1173 浏览 Are scoring sheets available for each crystallization condition Screening Suite? FAQ ID -1125 浏览 Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation? FAQ ID -1188 浏览 Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit? FAQ ID -1196 浏览 Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits? FAQ ID -1164 浏览 Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits? FAQ ID -1146 浏览 How are NeXtal deep-well blocks sealed? Can they be resealed? FAQ ID -1128 浏览 Does the EasyXtal Tool conform to an SBS format? FAQ ID -1115 浏览 Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks? FAQ ID -1160 浏览 How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools? FAQ ID -1113 浏览 Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint? FAQ ID -1167 浏览 How did you determine that your AllStar Negative Control siRNA binds to RISC? FAQ ID -1180 浏览 How is the quality of the crystallization condition Screening Suites guaranteed? FAQ ID -1126 浏览 How do I use EasyXtal Crystallization Tools? FAQ ID -1105 浏览 How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease? FAQ ID -1109 浏览 Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit? FAQ ID -1190 浏览 Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples? FAQ ID -1174 浏览 Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol? FAQ ID -1186 浏览 Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports? FAQ ID -1108 浏览 Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1? FAQ ID -1131 浏览 Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments? FAQ ID -1165 浏览 Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments? FAQ ID -1166 浏览 Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1? FAQ ID -1168 浏览 Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids? FAQ ID -2890 浏览 Which analyses can be performed with the PyroMark Q96 ID software version 1.0? FAQ ID -2845 浏览 Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines? FAQ ID -2887 浏览 How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin? FAQ ID -2895 浏览 Can I use my own siRNA design? FAQ ID -2897 浏览 Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827 浏览 Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector? FAQ ID -2896 浏览 Can I directly transfect SureSilencing shRNA Plasmids into my cells? FAQ ID -2899 浏览 Do any of the SureSilencing shRNA Plasmids contain inducible promoters? FAQ ID -2888 浏览 Can PyroMark Gold reagents be vortexed? FAQ ID -2844 浏览 What are the features of the new PyroMark Q96 ID software 2.5? FAQ ID -2867 浏览 What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids? FAQ ID -2885 浏览 Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene? FAQ ID -2882 浏览 What else do I need to complete an experiment using the SureSilencing shRNA Plasmids? FAQ ID -2889 浏览 What cell lines can I use with the SureSilencing shRNA Plasmids? FAQ ID -2891 浏览 How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes? FAQ ID -2893 浏览 Is the expression level of miRNA precursors lower compared to that of mature miRNAs? FAQ ID -2807 浏览 How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865 浏览 What is the PyroMark Q96 Data Converter? FAQ ID -2868 浏览 What is the pyrosequencing data exchange tool for? FAQ ID -2864 浏览 What is the composition of Buffer APP? FAQ ID -2804 浏览 What is the composition of Buffer FRN? FAQ ID -2802 浏览 What is the composition of Buffer APL? FAQ ID -2803 浏览 Which analyses can be performed with the PyroMark Q96 MD software? FAQ ID -2866 浏览 Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836 浏览 What is the SureSilencing shRNA Plasmids guarantee? FAQ ID -2898 浏览 What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids? FAQ ID -2886 浏览 Does the PyroMark CpG LINE assay target mouse transposons as well? -1 浏览 How do I find the SureSilencing shRNA Plasmids for my gene of interest? FAQ ID -2883 浏览 How are the SureSilencing shRNA Plasmids shipped? FAQ ID -2884 浏览 What is the concentration of SureENTRY Transduction Reagent? FAQ ID - 3708 浏览 Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate? FAQ ID - 3710 浏览 Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately? FAQ ID - 3712 浏览 What sample tubes can I use on my QIAsymphony SP system? FAQ ID - 3713 浏览 How can I decontaminate my QIAsymphony SP / AS system? FAQ ID - 3714 浏览 Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)? FAQ ID - 3717 浏览 How is the Pyromark Q48 cartridge to be cleaned? FAQ ID - 3718 浏览 Can the disc and absorber strips for the Pyromark Q48 be reused? FAQ ID - 3720 浏览 What is the dead volume of the cartridges in PyroMark Q48? FAQ ID - 3719 浏览 How much DNA is needed in the PyroMark Q48? FAQ ID - 3721 浏览 Can I use PyroMark Q48 for diagnostics? FAQ ID - 3722 浏览 Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep? FAQ ID - 3723 浏览 Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control? FAQ ID - 3724 浏览 Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit? FAQ ID - 3725 浏览 Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately? FAQ ID - 3726 浏览 Does QIAGEN offer an anti-GFP gapmer control? FAQ ID - 3739 浏览 Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1? FAQ ID - 3745 浏览 How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C? FAQ ID - 3744 浏览 Do I need to buy special kits for the QIAcube Connect? FAQ ID - 141485 浏览 Can I program my own protocols for the QIAcube Connect? FAQ ID - 141486 浏览 Can the QIAcube Connect rotor and/or buckets be removed for cleaning? FAQ ID - 141491 浏览 Can purification columns from other suppliers be processed on the QIAcube Connect? FAQ ID - 141487 浏览 How can I get software updates for the QIAcube Connect? FAQ ID - 141494 浏览 Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect? FAQ ID - 141496 浏览 Do I need to discard partially used QIAcube Connect tip racks? FAQ ID - 141499 浏览 How can I decontaminate the QIAcube Connect system? FAQ ID - 141500 浏览 Can the QIAcube Connect heater/shaker be used independently from protocol runs? FAQ ID - 141502 浏览 How can I load new protocols onto the QIAcube Connect? FAQ ID - 141501 浏览 What dedicated QIAcube Connect kits are available? FAQ ID - 141504 浏览 Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect? FAQ ID - 1414507 浏览 Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings? FAQ ID - 141505 浏览 Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect? FAQ ID - 141506 浏览 Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? FAQ ID - 141508 浏览 How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect? FAQ ID - 141509 浏览 Can the CB and RB sample tubes be used interchangeably? FAQ ID - 141510 浏览 Which network settings are supported by the QIAcube Connect and Q-Base? FAQ ID - 141517 浏览 Why can I not find the Q-Base device in the device list during the first installation? FAQ ID - 141516 浏览 Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App? FAQ ID - 1414518 浏览 Why is the QIAcube Connect App reacting so slowly? FAQ ID - 141519 浏览 Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)? FAQ ID - 1414520 浏览 Why does the connection test fail? FAQ ID - 141522 浏览 How can I accurately determine the amount of input material for the EpiTect Hi-C Kit? FAQ ID - 143065 浏览 What sample source do you support? FAQ ID - 143066 浏览 How long does it take to complete the EpiTect Hi-C protocol? FAQ ID - 143068 浏览 How many reactions are in a kit? FAQ ID - 143067 浏览 Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol? FAQ ID - 143069 浏览 Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease? FAQ ID - 143070 浏览 Are there stopping points in the Hi-C workflow? FAQ ID - 143071 浏览 With which platform can I perform my sequencing? FAQ ID - 143072 浏览 With which read length should the EpiTect Hi-C NGS libraries be sequenced FAQ ID - 143073 浏览 How do I know if my Hi-C sample is a good candidate for deep sequencing? FAQ ID - 143074 浏览 What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform? FAQ ID - 143076 浏览 Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments? FAQ ID - 143078 浏览 How critical is the time of the temperature change from 60°C to 80°C? FAQ ID - 143768 浏览 Is it possible to use Lyse&Spin baskets? FAQ ID - 143762 浏览 Why is DTT used only for semen samples? FAQ ID - 143761 浏览 Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells? FAQ ID - 143079 浏览 Can the lysis volume be reduced for higher sensitivity? FAQ ID - 143763 浏览 What if only one heater shaker is available? FAQ ID - 143764 浏览 Why is there no male DNA obtained from sexual assault samples? FAQ ID - 143767 浏览 If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.) FAQ ID - 143766 浏览 Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable? FAQ ID -147395 浏览 What RNAs has QIAseq FastSelect been designed to remove? FAQ ID -147910 浏览 How does QIAseq FastSelect work? FAQ ID -147907 浏览 Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature? FAQ ID -147909 浏览 Will QIAseq FastSelect work in other species? FAQ ID -147911 浏览 What library prep kits has QIAseq FastSelect been tested with? FAQ ID -147912 浏览 I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit? FAQ ID -147913 浏览 What RNA range has the QIAseq FastSelect been tested with? FAQ ID -147914 浏览 Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S? FAQ ID -147915 浏览 Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.? FAQ ID -147916 浏览 Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription? FAQ ID -1 浏览 How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32 浏览 When is chloramphenicol amplification of plasmids performed? FAQ ID -3 浏览 What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit? FAQ ID -45 浏览 What are the most commonly used protease inhibitors? FAQ ID -53 浏览 How should I propagate pQE expression plasmids? FAQ ID -58 浏览 How can I increase the amount of soluble recombinant protein in E. coli expression? FAQ ID -64 浏览 How can I remove imidazole from a protein sample? FAQ ID -91 浏览 Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97 浏览 How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28 浏览 How should I store plant material for DNA isolation using the DNeasy Plant Kit? FAQ ID -114 浏览 Do I need to use an RNase inhibitor in my RT reaction? FAQ ID -119 浏览 Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? FAQ ID -127 浏览 Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup? FAQ ID -130 浏览 How does imidazole affect my quantitation of protein? FAQ ID -132 浏览 Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel? FAQ ID -133 浏览 What is the difference between disruption and homogenization in the RNeasy System? FAQ ID -139 浏览 What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips? FAQ ID -142 浏览 Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins? FAQ ID -147 浏览 What is the genotype of the EZ Competent Cells? FAQ ID -157 浏览 What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit? FAQ ID -160 浏览 What are the molecular weights of proteins in the 6xHis Protein Ladder? FAQ ID -169 浏览 How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent? FAQ ID -176 浏览 How can I improve recoveries when using the QIAquick Kits? FAQ ID -180 浏览 How can I improve transfection efficiency using Effectene Transfection Reagent? FAQ ID -181 浏览 What is the principle behind Effectene Transfection Reagent? FAQ ID -184 浏览 What is the composition of Buffer EB? FAQ ID -199 浏览 Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps? FAQ ID - 3709 浏览 Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C? FAQ ID - 3711 浏览 What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits? FAQ ID - 3715 浏览 Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)? FAQ ID - 3716 浏览 How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023 浏览 What is the recipe for 1x PBS solution? FAQ ID -1030 浏览 Where can I find a protocol for cleanup of already purified plasmid DNA? FAQ ID -1031 浏览 How do I perform an Acetone Precipitation for concentrating and desalting protein samples? FAQ ID -1035 浏览 What is the difference between Buffers RLT and RLT Plus? FAQ ID -1043 浏览 What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit? FAQ ID -1047 浏览 Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel? FAQ ID -1051 浏览 Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase? FAQ ID -1052 浏览 How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits? FAQ ID -1061 浏览 What should I use as a standard for absolute quantification in real-time PCR? FAQ ID -1085 浏览 What does transcript variant mean when searching for specific QuantiTect Primer Assays? FAQ ID -1137 浏览 What is a QuantiTect Primer Assay? FAQ ID -1141 浏览 Do QuantiTect Primer Assays contain SYBR Green dye? FAQ ID -1143 浏览 What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II? FAQ ID -1218 浏览 What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit? FAQ ID -1220 浏览 What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II? FAQ ID -1221 浏览 Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA? FAQ ID -1250 浏览 Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA? FAQ ID -1251 浏览 What is the pH value of QIAGEN's Nuclease-Free Water? FAQ ID -1290 浏览 Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 浏览 Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA? FAQ ID -1328 浏览 Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time? FAQ ID -1352 浏览 How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit? FAQ ID -1354 浏览 What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit? FAQ ID -1356 浏览 How do I resuspend my FlexiPlate siRNAs? FAQ ID -1360 浏览 Are there any specific cleaning solutions recommended for the QIAcube? FAQ ID -1416 浏览 Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 浏览 Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1577 浏览 What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1580 浏览 What is the binding capacity of the Ni-NTA Superflow Cartridges? FAQ ID -1603 浏览 How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644 浏览 Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer? FAQ ID -1659 浏览 Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs? FAQ ID -1664 浏览 What is the composition of Buffer PE? FAQ ID -1691 浏览 What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738 浏览 How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced? FAQ ID -1838 浏览 Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer? FAQ ID -1848 浏览 What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID -1852 浏览 If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated? FAQ ID -1858 浏览 What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901 浏览 Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913 浏览 How much sample can be used with the RNeasy Plus 96 Kit? FAQ ID -1992 浏览 What is the composition of Buffer P2? FAQ ID -203 浏览 A white precipitate has formed in my 10x RT buffer. Is it still ok to use? FAQ ID -216 浏览 For how long are BioMag Particles stable? FAQ ID -262 浏览 I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing? FAQ ID -263 浏览 Can I clean up my DNase treated RNA samples using RNeasy columns? FAQ ID -286 浏览 How can one determine the optimal annealing temperature for a specific PCR assay? FAQ ID -288 浏览 What is the composition of the QIAGEN Multiplex PCR Buffer? FAQ ID -289 浏览 Can I use HEPES buffer instead of phosphate in my Ni-NTA column? FAQ ID -291 浏览 Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit? FAQ ID -293 浏览 What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit? FAQ ID -2004 浏览 What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029 浏览 How can a C-terminal 6xHis-tagged QIAgene be expressed? FAQ ID -2041 浏览 Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 浏览 Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results? FAQ ID -2079 浏览 Does the Rotor-Gene Q software have the capacity to export data? FAQ ID -2089 浏览 Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 浏览 Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163 浏览 Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types? FAQ ID -2190 浏览 How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192 浏览 Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 浏览 Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 浏览 What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2203 浏览 What buffer conditions give the best resolution for agarose gel electrophoresis? FAQ ID -2256 浏览 How much DNA should be loaded per well of an agarose gel? FAQ ID -2259 浏览 Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 浏览 What is the "seed sequence" of a miRNA? FAQ ID -2262 浏览 Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits? FAQ ID -2301 浏览 Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit? FAQ ID -2315 浏览 How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit? FAQ ID -2340 浏览 How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? FAQ ID -2341 浏览 How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? FAQ ID -2350 浏览 Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? FAQ ID -2371 浏览 Can I buy QIAquick and MinElute columns separately? FAQ ID -2460 浏览 What is the PAXgene 96 filter plate? FAQ ID -2486 浏览 Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube? FAQ ID -2509 浏览 Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System? FAQ ID -2514 浏览 How long is the fixation time? FAQ ID -2519 浏览 What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits? FAQ ID -2543 浏览 Are there any accessories required for sample disruption with the TissueLyser LT? FAQ ID -2551 浏览 Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit? FAQ ID -2603 浏览 During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit FAQ ID -2607 浏览 What is the SBTengine® software? FAQ ID -2628 浏览 How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment? FAQ ID -2654 浏览 What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis? FAQ ID -2659 浏览 How can I avoid or remove genomic DNA contamination from the total RNA preparation? FAQ ID -2662 浏览 What are the differences between one-step and two-step RT--PCR? FAQ ID -2666 浏览 What is qPCR? FAQ ID -2668 浏览 What are the common primer and probe chemistries utilized for qPCR assays? FAQ ID -2669 浏览 What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes? FAQ ID -2671 浏览 What negative controls are typically included in qPCR and/or qRT-PCR experiments? FAQ ID -2672 浏览 What positive controls are typically included in qPCR and/or qRT-PCR experiments? FAQ ID -2673 浏览 What are the guidelines for choosing a housekeeping gene for normalizing qPCR results? FAQ ID -2674 浏览 Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays? FAQ ID -2675 浏览 How does HotStart PCR help minimize nonspecific amplification events? FAQ ID -2676 浏览 What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry? FAQ ID -2678 浏览 What is the delta Rn value? FAQ ID -2681 浏览 What is the threshold cycle or Ct value? FAQ ID -2682 浏览 Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay? FAQ ID -2684 浏览 Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay? FAQ ID -2685 浏览 Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay? FAQ ID -2686 浏览 Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase? FAQ ID -2689 浏览 Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC? FAQ ID -2690 浏览 What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed? FAQ ID -2691 浏览 What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach? FAQ ID -2692 浏览 What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed? FAQ ID -2693 浏览 How do I determine the amplification efficiency of my qPCR assay? FAQ ID -2694 浏览 How do I determine the linear dynamic range of my qPCR or qRT-PCR assay? FAQ ID -2696 浏览 What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them? FAQ ID -2705 浏览 What do I need to complete a RT² qPCR Primer Assay? FAQ ID -2707 浏览 How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 浏览 What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 浏览 How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 浏览 How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 浏览 At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 浏览 What is RNA interference (RNAi)? FAQ ID -2755 浏览 What are the key factors for success in an RNAi experiment? FAQ ID -2758 浏览 What criteria should one use in choosing between siRNA versus shRNA for their studies? FAQ ID -2771 浏览 How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 浏览 What are the SureSilencing shRNA Plasmids? FAQ ID -2784 浏览 What is the composition of Buffer S3? FAQ ID -2788 浏览 What is the composition of Buffer ETR? FAQ ID -2789 浏览 What is the composition of Buffer BB? FAQ ID -2790 浏览 What is the composition of Buffer RLT plus? FAQ ID -2794 浏览 What is the composition of Buffer RLC? FAQ ID -2795 浏览 What is the composition of Buffer RPE? FAQ ID -2797 浏览 What is the composition of Buffer RWT? FAQ ID -2798 浏览 What is the composition of gDNA Eliminator Solution? FAQ ID -2799 浏览 What is the composition of Buffer RDD? FAQ ID -2800 浏览 What is the composition of Buffer PKD? FAQ ID -2801 浏览 What is the composition of Buffer ALO? FAQ ID -2805 浏览 What is the composition of RBC Lysis Solution? FAQ ID -2808 浏览 What is the composition of Cell Lysis Solution? FAQ ID -2809 浏览 What is the composition of Protein Precipitation Solution? FAQ ID -2810 浏览 What is the composition of Cell Suspension Solution? FAQ ID -2811 浏览 What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits? FAQ ID -2812 浏览 What is the composition of DNA Hydration Solution in Gentra Puregene Kits? FAQ ID -2813 浏览 What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841 浏览 What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0? FAQ ID -2849 浏览 Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest? FAQ ID -2894 浏览 Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -2926 浏览 What is the composition of Buffer G2? FAQ ID -2943 浏览 What is the composition of Buffer B1? FAQ ID -2944 浏览 What is the composition of Buffer B2? FAQ ID -2945 浏览 How much DNA does a human cell contain? FAQ ID -2948 浏览 How much RNA does a bacterial cell contain? FAQ ID -2949 浏览 How much DNA and RNA can be expected from human blood cells? FAQ ID -2950 浏览 What is the cellular composition of human blood? FAQ ID -2951 浏览 How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation? FAQ ID -2954 浏览 Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers? FAQ ID -2980 浏览 Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2987 浏览 What is the sequence of the miScript Universal Primer? FAQ ID -2994 浏览 How can I keep my centrifuge tubes endotoxin-free? FAQ ID -301 浏览 Can Buffers N3 and P3 be used interchangeably? FAQ ID -310 浏览 Are QIAprep and QIAquick Spin columns interchangeable? FAQ ID -311 浏览 Can I store agarose gel slices containing DNA for gel extraction at a later point? FAQ ID -313 浏览 How can QIAGEN Protease and Proteinase K be inactivated? FAQ ID -315 浏览 Does the 5x OneStep RT-PCR Buffer contain BSA? FAQ ID -326 浏览 What is the origin of replication and the plasmid copy number of the pQE vectors? FAQ ID -338 浏览 Is QIAGEN carrier RNA sold separately? FAQ ID -351 浏览 What is the white insoluble precipitate in my resuspended plasmid DNA pellet? FAQ ID -352 浏览 What is the composition of PBS? FAQ ID -361 浏览 How can I express toxic protein in E. coli? FAQ ID -373 浏览 How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? FAQ ID -374 浏览 What are the dissociation constants for the Anti-His Antibodies? FAQ ID -385 浏览 What is the average molecular weight of a siRNA, and how do I convert uM to ug values? FAQ ID -388 浏览 Can siRNA silence bacterial genes? FAQ ID -395 浏览 How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit? FAQ ID -3003 浏览 Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples? FAQ ID -3032 浏览 Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits? FAQ ID -3053 浏览 Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings? FAQ ID -3054 浏览 What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced? FAQ ID -3055 浏览 What is the thermal output for a BioRobot MDx and BioRobot Universal? FAQ ID -3056 浏览 What is the thermal output for a QIAsymphony SP and AS instrument? FAQ ID -3057 浏览 How precise is the QIAgility? FAQ ID -3063 浏览 What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility? FAQ ID -3064 浏览 How long does it take to set up a 96-well plate PCR reaction on the QIAgility? FAQ ID -3066 浏览 Can I use a bar code scanner or reader on the QIAgility? FAQ ID -3067 浏览 How can I find the run parameters for a BioSprint protocol that I am running? FAQ ID -3069 浏览 Can BioSprint 96 be connected to LIMS? FAQ ID -3070 浏览 What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit? FAQ ID -3081 浏览 What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits? FAQ ID -3082 浏览 What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols? FAQ ID -3083 浏览 What is the concentration of the different Proteinase K solutions sold by QIAGEN? FAQ ID -3084 浏览 Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit? FAQ ID -3085 浏览 What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)? FAQ ID -3086 浏览 How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 浏览 Can we do modification XXXXX for custom siRNA that is not on the web page? FAQ ID -3107 浏览 What is the stability of siRNA lyophilized and resuspended? FAQ ID -3109 浏览 Do you offer FlexiTube or FlexiPlate siRNAs for rat? FAQ ID -3114 浏览 Is it possible to extract RNA from frozen blood? FAQ ID -3119 浏览 Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 浏览 What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails -48 浏览 Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit? FAQ ID -3125 浏览 Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor? FAQ ID -3132 浏览 How do I remove the RNA contamination from purified DNA? FAQ ID -3133 浏览 What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini? FAQ ID -3140 浏览 What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ? FAQ ID -3169 浏览 What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs? FAQ ID -3174 浏览 What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed? FAQ ID -3175 浏览 What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q? FAQ ID -3178 浏览 Is REST software Windows 7 compatible? FAQ ID -3182 浏览 Can the Rotor-Gene Q perform gradient PCR? FAQ ID -3183 浏览 What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 浏览 What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 浏览 What is LLOQ? FAQ ID — 3401 浏览 What is the difference between LLOQ and LOD? FAQ ID — 3402 浏览 What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays? FAQ ID - 3427 浏览 What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit? FAQ ID - 3429 浏览 Can the miScript II RT Kit be used to quantify plant miRNAs? FAQ ID - 3431 浏览 What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system? FAQ ID - 3444 浏览 Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation? FAQ ID - 3467 浏览 What is the proper disposal procedure for waste from the sample preparation? FAQ ID - 3470 浏览 What is the expected yield from 8.5 ml of blood? FAQ ID - 3496 浏览 What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain? FAQ ID - 3501 浏览 How should blood samples drawn into PAXgene Blood DNA Tubes be transported? FAQ ID - 3507 浏览 How long can samples in PAXgene Blood DNA Tubes be stored at room temperature? FAQ ID - 3508 浏览 What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method? FAQ ID - 3510 浏览 Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3512 浏览 What does PAXgene Tissue STABILIZER contain? FAQ ID - 3513 浏览 What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield? FAQ ID - 3516 浏览 Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing? FAQ ID - 3517 浏览 What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit? FAQ ID - 3519 浏览 Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material? FAQ ID - 3521 浏览 Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library? FAQ ID - 3534 浏览 Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used? FAQ ID - 3559 浏览 Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3573 浏览 Is clot detection possible on the QIAsymphony? FAQ - ID 3588 浏览 How can I determine if a tube is suitable for clot detection on QIAsymphony? FAQ ID - 3590 浏览 How should I thaw frozen plasma samples? FAQ ID - 3645 浏览 How do Unique Molecular Indexes (UMIs) improve quantification? FAQ ID - 3669 浏览 Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)? FAQ ID - 3686 浏览 I would like to stop during the library preparation procedure. When can I do that? FAQ ID - 3687 浏览 Which regions are covered by the GR DNAseq V2 Panels (#181900)? FAQ ID - 3688 浏览 When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off? FAQ ID - 3690 浏览 How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber? FAQ ID - 3691 浏览 Are peak heights of Q48 comparable to other PyroMark instruments? FAQ ID - 3692 浏览 How much space does the PyroMark Q48 Autoprep instrument need? FAQ ID - 3693 浏览 Can unused wells in a pyrosequencing discs be used in the next run? FAQ ID - 3694 浏览 Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)? FAQ ID - 3695 浏览 What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)? FAQ ID - 3696 浏览 What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center? FAQ ID - 3698 浏览 How do I prepare Buffer MP? FAQ ID - 3620 浏览 Are the QIAshredder columns included in various QIAGEN kits the same? FAQ ID - 3624 浏览 What is the composition of buffer QBT? FAQ ID -411 浏览 What is the composition of buffer QC? FAQ ID -412 浏览 What is the composition of buffer QF? FAQ ID -413 浏览 What is the composition of buffer STE? FAQ ID -415 浏览 What is the composition of buffer TE? FAQ ID -416 浏览 What is the composition of buffer FWB2? FAQ ID -417 浏览 What is the composition of buffer P3? FAQ ID -418 浏览 What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors? FAQ ID -470 浏览 Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 浏览 Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed? FAQ ID -474 浏览 Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit? FAQ ID -482 浏览 Can Ni-NTA resins be used to purify protein with an internal His-tag? FAQ ID -496 浏览 How do I calculate the percentage of silencing with real-time RT-PCR for siRNA? FAQ ID -498 浏览 Do you have a protocol for the removal of endotoxins from already purified plasmid DNA? FAQ ID -500 浏览 Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit? FAQ ID -518 浏览 What is the composition of the siRNA resuspension & annealing buffer? FAQ ID -522 浏览 Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls? FAQ ID -523 浏览 What is Tissue-Tek O.C.T., and what is it used for? FAQ ID -530 浏览 Why do replicates in real-time PCR have different plateau heights? FAQ ID -539 浏览 How can I avoid primer-dimer formation during PCR amplification? FAQ ID -544 浏览 How can I tell if I have primer-dimers in my PCR reaction? FAQ ID -552 浏览 How should I store cDNA produced using Omniscript Reverse Transcriptase? FAQ ID -561 浏览 Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits? FAQ ID -563 浏览 What is the composition of Buffer ER? Is it available separately? FAQ ID -571 浏览 What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried? FAQ ID -572 浏览 Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit? FAQ ID -575 浏览 Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? FAQ ID -577 浏览 What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit? FAQ ID -600 浏览 Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit? FAQ ID -603 浏览 What do you recommend for the cleanup of genomic DNA (gDNA)? FAQ ID -618 浏览 How can I improve my RNA yield from liver sample when using RNeasy Kits? FAQ ID -623 浏览 Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100 浏览 What 96-well plates do you recommend for use with the LiquiChip Workstation? FAQ ID -630 浏览 What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample? FAQ ID -631 浏览 Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? FAQ ID -633 浏览 Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing? FAQ ID -637 浏览 What is the shelf-life of my QIAGEN Kit? FAQ ID -651 浏览 What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification? FAQ ID -665 浏览 Can I purchase Phi29 DNA polymerase only? FAQ ID -708 浏览 What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit? FAQ ID -721 浏览 I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20 浏览 I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745 浏览 How much DNA is obtained in the average PCR reaction? FAQ ID -750 浏览 Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA? FAQ ID -754 浏览 Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns? FAQ ID -759 浏览 How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN? FAQ ID -762 浏览 What is the difference between Ni-NTA Agarose and Ni-NTA Superflow? FAQ ID -764 浏览 What is the composition of Buffer N3? FAQ ID -767 浏览 Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa? FAQ ID -770 浏览 How should fluorescent labeled probes be stored? FAQ ID -784 浏览 Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA? FAQ ID -785 浏览 Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable? FAQ ID -786 浏览 How can I get specific information and a quote for my individual Sequencing Service needs? FAQ ID -792 浏览 Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells? FAQ ID -795 浏览 How can I separate PCR fragments that are small and very close in size on an agarose gel? FAQ ID -800 浏览 Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins? FAQ ID -802 浏览 What Disinfectant Solution do you recommend for the BioRobot MDx? FAQ ID -809 浏览 Is the E. coli Host Strain M15[pREP4] resistant to Zeocin? FAQ ID -842 浏览 What annealing temperature should be used with the QuantiTect Primer Assays? FAQ ID -849 浏览 Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 浏览 Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent? FAQ ID -865 浏览 Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis? FAQ ID -879 浏览 Do you have a protocol for the isolation of genomic DNA from frozen clotted blood? FAQ ID -900. Test 浏览 Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)? FAQ ID -907 浏览 Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908 浏览 What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy? FAQ ID -942 浏览 Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA? FAQ ID -959 浏览 Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates? FAQ ID -971 浏览 Do you have a protocol for the purification of PCR products using the BioSprint System? FAQ ID -984 浏览 Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent? FAQ ID -997 浏览 How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE? FAQ ID - 3707 浏览 How can I increase expression of my 6xHis-tagged protein in E. coli? FAQ ID -63 浏览 Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit? 148723 浏览 What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit? 148724 浏览 What is the composition of buffer OW2? FAQ ID -419 浏览 What is the composition of buffer OBB? FAQ ID -421 浏览 Do I need to buy special kits for the QIAcube Connect MDx? 148725 浏览 What applications are offered on the QIAcube Connect MDx instrument? 148726 浏览 Can I program my own protocols for the QIAcube Connect MDx? 148727 浏览 Can purification columns from other suppliers be processed on the QIAcube Connect MDx? 148728 浏览 What are the dimensions and weight of the QIAcube Connect MDx? 148729 浏览 Can the QIAcube rotor adapters be autoclaved? 148730 浏览 Can the QIAcube rotor and/or buckets be removed for cleaning? 148731 浏览 Can the QIAcube be connected to a laboratory information management system? 148732 浏览 Are run reports and/or log files available on the QIAcube? 148733 浏览 How can I get software updates for the QIAcube Connect MDx? 148734 浏览 Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion? 148735 浏览 Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? 148736 浏览 Do I need to discard partially used QIAcube tip racks? 148738 浏览 How can I decontaminate the QIAcube Connect MDx system? 148739 浏览 How can I load new protocols onto the QIAcube Connect MDx? 148740 浏览 Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs? 148741 浏览 Can the QIAcube Connect MDx centrifuge be used independently from protocol runs? 148742 浏览 What dedicated QIAcube Kits are available? 148743 浏览 Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument? 148744 浏览 Can I run life science and custom protocols on a QIAcube Connect MDx? 148745 浏览 What are the heating/cooling rates in the QIAquant instruments? 153919 浏览 Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results? 153920 浏览 What sample volume is suitable for use in the QIAquant? 153921 浏览 What types of reaction vessels are required for use in the QIAquant? 153922 浏览 Is the QIAquant equipped with a temperature gradient function? 153923 浏览 Are the 96- and 384-sample blocks interchangeable? 153924 浏览 Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel? 153925 浏览 How many molecules can be detected in the system? 153926 浏览 What applications can the QIAquant be used for? 153927 浏览 Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels? 153928 浏览 Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument? 153929 浏览 Is the software of the QIAquant compatible with 21CFR part11? 153930 浏览 Is it possible to import a standard curve from a previous run? 153947 浏览 Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running? 153948 浏览 Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384? 153950 浏览 Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously? 153951 浏览 Is it possible to establish an internet connection with QIAquant instrument? 153952 浏览 Can I connect more than one QIAquant instrument to the same computer? 153953 浏览 What is the algorithm behind data normalization in the QIAquant Software? 153954 浏览 Is it possible to edit the sample sheet after the run in the QIAquant Software? 153955 浏览 What are the LIMS capacity of the software? 153949 浏览 Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export? 153946 浏览 I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them? FAQ ID - 3560 浏览 Where can I find ScreenGel profiles for the Large Fragment Marker Kit? 154189 浏览 Do I need to dilute the size marker? 154191 浏览 Can I start a QIAxcel run without using a process profile? 154188 浏览 There is n/a as peak assignment instead of the size of my fragments. How can I solve this? 154190 浏览 Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing? 153891 浏览 What are recommended stopping points in the procedure of the QIAamp DNA FFPE Advanced Kits? 153892 浏览 Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure? 153893 浏览 Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C? 153894 浏览 Can the 2nd Prot K step be omitted? 153896 浏览 What size of DNA can be expected? 153897 浏览 What is the difference of buffer FTB versus Buffer ATL? 153895 浏览 Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments? 3761 浏览 How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite? 3762 浏览 Can I see error codes on the instrument touchscreen? 3763 浏览 How are results stored within the QIAcuity Software Suite? 3764 浏览 What is the scope of a regular maintenance of the QIAcuity? 3765 浏览 Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex? 3766 浏览 Can I use an empty nanoplate without seal to perform a dry run? 3767 浏览 Does an incorrect handling of a plate might affect the results? 3768 浏览 What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF? 3769 浏览 Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not? 3770 浏览 Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020? 3771 浏览 How can I get the latest VPF (Volume Precision Factor)? 3772 浏览 Where can I find assays specifically designed for QIAcuity? 3773 浏览 Can I prepare a dPCR reaction directly in QIAcuity Nanoplate? 3774 浏览 Can I use the dPCR master mix in a qPCR cycler for optimization purposes? 3775 浏览 What is the purpose of the reference channel in QIAcuity? 3776 浏览 How to prepare DNA prior to dPCR? 3778 浏览 Can I use a custom master mix instead of a QIAGEN master mix? 3777 浏览 Which sample preparation method is recommended for QIAcuity dPCR? 3779 浏览 What are the storage conditions and expiry date of QIAcuity consumables? 3780 浏览 What is the input volume for QIAcuity Nanoplate well? 3782 浏览 Can I use the QIAcuity Nanoplate in more than one runs? 3781 浏览 What is the VPF (Volume Precision Factor)? 3784 浏览 When and how often do I need a new VPF (Volume Precision Factor)? 3785 浏览 Is a standard curve needed in dPCR? 3786 浏览 Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design? 3788 浏览 Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine? 3791 浏览 Is there something to consider when working with transduced cells treated with Polybrene? 3790 浏览 For sequencing application, does the lysate require purification? 3792 浏览 Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated? 3793 浏览 Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing? 3794 浏览 Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol? 3795 浏览 What is the minimum cell number needed for cell lysis? 3789 浏览 For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided? 3796 浏览 What is the sequence of the QIAseq miRNA NGS 5’ Adapter? FAQ ID - 3672 浏览 What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits? 3753 浏览 Are there other options for paraffin removal? 3754 浏览 Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure? 3755 浏览 Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C? 3756 浏览 What is the difference between Buffer FTB and Buffer ATL? 3757 浏览 Can the 2nd Proteinase K step be omitted? 3758 浏览 What size of DNA can be expected? 3759 浏览 Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing? 3760 浏览 Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis? FAQ ID -1056 浏览 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 浏览 Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 浏览 Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 浏览 When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 浏览 Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 浏览 CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 浏览 What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 浏览 Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 浏览 I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 浏览 What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 浏览 What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 浏览 Do you sell CoralLoad dye separately? FAQ ID - 3368 浏览 Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 浏览 How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at 2–8°C? FAQ ID - 3379 浏览 Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)? FAQ ID - 3384 浏览 What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 浏览 Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 浏览 Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 浏览 Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 浏览 Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 浏览 Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 浏览 What is the RNase A concentration and composition of Buffer P1? FAQ ID -198 浏览 Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)? 3797 浏览 Where can I download the protocol for the KingFisher Flex? 3798 浏览 Who can help in case of issues with the third party instrument? 3799 浏览 Is the virus inactivated during the procedure? 3800 浏览 Can I use sample volumes other than 300 µl? 3801 浏览 Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry? 3802 浏览 I would like to use the kit on another third party instrument. Who should I contact? 3803 浏览 How long does a saliva collection take? 3804 浏览 What can I do to stimulate saliva flow? 3805 浏览 Can I stop saliva collection before the fill line is reached? 3806 浏览 What can I do if I don't see the fill line anymore due to saliva bubbles? 3807 浏览 What happens if I accidentally unscrew the funnel before saliva collection is completed? 3808 浏览 May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely? 3809 浏览 What is in the additive in the PAXgene Saliva Collector? 3810 浏览 What is the shelf life of unused PAXgene Saliva Collectors? 3811 浏览 How should unused PAXgene Saliva Collector be stored? 3812 浏览 What should I avoid before sample collection? 3813 浏览 How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva? 3814 浏览 How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature? 3815 浏览 Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C? 3816 浏览 Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C? 3817 浏览 Is RNA preserved in saliva collected in PAXgene Saliva Collector? 3818 浏览 Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector? 3819 浏览 What should I do if stabilized saliva samples are very heterogenous? 3820 浏览 What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector? 3823 浏览 Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument? 3824 浏览 Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation? 3826 浏览 Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector? 3827 浏览 Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector? 3828 浏览 Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity? 3829 浏览 Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack? 3825 浏览 What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector? 3822 浏览 Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector? 3821 浏览 What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments? FAQ ID - 3837 浏览 Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits? FAQ ID - 3836 浏览 What is the sequence of the UDI 5' Adapter? FAQ ID - 3835 浏览 Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit? FAQ ID - 3674 浏览 What can be used to validate results obtained with the QIAseq miRNA Library Kit? FAQ ID - 3675 浏览 What is the maximum number of available sample indexes? FAQ ID - 3665 浏览 What are recommended stopping points during the QIAseq miRNA Library Kit procedure? FAQ ID - 3663 浏览 Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit? FAQ ID - 3659 浏览 What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit? FAQ ID - 3668 浏览 What is the acceptable number of reads per sample per miRNA sequencing run? FAQ ID - 3673 浏览 Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays? FAQ ID -1169 浏览 Is training of lab personnel included with the purchase of a BioRobot EZ1? FAQ ID -1239 浏览 Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot? FAQ ID -1240 浏览 How can we get our BioRobot EZ1 upgraded when new protocols are launched? FAQ ID -1241 浏览 Where can I find information about the worktable setup on the BioRobot EZ1? FAQ ID -1243 浏览 What disposables are required for nucleic acid isolations on the BioRobot EZ1? FAQ ID -1246 浏览 How should I prepare buffy coat samples for use on the BioRobot EZ1? FAQ ID -1249 浏览 Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1? FAQ ID -985 浏览 What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer? FAQ ID -528 浏览 Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1? FAQ ID -975 浏览 Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1? FAQ ID -976 浏览 Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1? FAQ ID -992 浏览 Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)? FAQ ID -1025 浏览 What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)? FAQ ID - 3838 浏览 What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-? FAQ ID - 3839 浏览 What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-? FAQ ID - 3840 浏览 What PCR enzymes can be used following the first-strand synthesis? FAQ ID - 3841 浏览 What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)? FAQ ID - 3842 浏览 What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24? 2857 浏览 Does the PyroMark LINE assay target all LINE sequences? 2860 浏览 What is the amplicon length of the PyroMark CpG LINE assay? 2856 浏览 How do I perform the PyroMark Q24 Advanced upgrade? FAQ ID -3163 浏览 Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system? FAQ ID -3160 浏览 What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents? FAQ ID -3165 浏览 How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094 浏览 We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions? FAQ ID - 3621 浏览 What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855 浏览 How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24? FAQ ID -2095 浏览 How long does a single run on the PyroMark Q24 take? FAQ ID -2096 浏览 3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram FAQ ID - 3843 浏览 When do I have to change the pulse settings/methods in a pyrosequencing run setup? FAQ ID -2942 浏览 What is a PyroMark instrument method or instrument code? FAQ ID -2941 浏览 Can the PyroMark Q96 CpG LINE assay be used with an ID system? 2861 浏览 How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused? FAQ ID -2863 浏览 How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage. FAQ ID -2878 浏览 Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872 浏览 What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875 浏览 Is the CpG software included in the PyroMark instruments to study methylation status? FAQ ID -2842 浏览 What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881 浏览 Where can I find explanations to the warning given by the PyroMark software after run data analysis? FAQ ID -2874 浏览 Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838 浏览 How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations? FAQ ID -2848 浏览 What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837 浏览 What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879 浏览 How many CpG sites are analyzed by the PyroMark CpG LINE assay? 2858 浏览 Is there a user manual available for the PyroMark Assay design software? FAQ ID -2851 浏览 Which operating system is compatible with PyroMark IdentiFire Software? FAQ ID - 3340 浏览 How do I set up a PyroMark CpG Assay? FAQ ID -2814 浏览 What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007 浏览 Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823 浏览 Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818 浏览 How are the PyroMark CpG Assays reconstituted? FAQ ID -2817 浏览 How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816 浏览 What are the features of PyroMark CpG Assays, for example, in terms of design and validation? FAQ ID -2821 浏览 What is included in a PyroMark Custom Assay? FAQ ID -2815 浏览 In which format can PyroMark CpG Assays be ordered? FAQ ID -2824 浏览 Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822 浏览 Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862 浏览 What is the recommended amplicon size for CpG assays? FAQ ID -2825 浏览 What kind of reading length can I expect when using pyrosequencing technology for sequence analysis? FAQ ID -2216 浏览 Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843 浏览 Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832 浏览 What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826 浏览 What is the concentration of PyroMark Control Oligo? FAQ ID -2846 浏览 What is the sample throughput of pyrosequencing systems? FAQ ID -2215 浏览 How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877 浏览 What is the sensitivity limitation for pyrosequencing? FAQ ID -2840 浏览 What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852 浏览 Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839 浏览 How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871 浏览 Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850 浏览 What are critical steps during CTC enrichment? 3830 浏览 How pure are the CTCs enriched from blood samples using the AdnaTest? 3831 浏览 Can frozen blood be used for the procedure? 3832 浏览 Can the EZ2 AdnaTest be used for other body fluids than blood? 3833 浏览 Can Heparin blood be used? 3834 浏览 Can the kit be used on the EZ2 Connect? FAQ ID - 3845 浏览 Can I use a different elution buffer? FAQ ID - 3847 浏览 What is the average amount of cfDNA present in 1 ml plasma? FAQ ID - 3846 浏览 Can the kit be used on the EZ1 Advanced XL? FAQ ID - 3844 浏览 Do you have a protocol for purification of cytoplasmic RNA from animal cells? FAQ ID -1257 浏览 What kits does QIAGEN offer to extract RNA from whole blood? FAQ ID -304 浏览 What is the composition of Buffer RLT? FAQ ID -2793 浏览 What is the key technical challenge in isolating high quality RNA from cell or tissue samples? FAQ ID -2656 浏览 Do you have a protocol for the isolation of RNA from leukocytes in milk? FAQ ID -952 浏览 What is the maximum binding capacity of RNeasy spin columns? FAQ ID -290 浏览 Can QIAquick Kits be used to clean up RNA samples? FAQ ID -490 浏览 Are RNeasy spin columns sold separately? FAQ ID -159 浏览 Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution? FAQ ID -619 浏览 Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues? FAQ ID -467 浏览 How can I minimize degradation of RNA from my pancreas sample? FAQ ID -624 浏览 What is the composition of Buffer RW1? FAQ ID -2796 浏览 Do you have a protocol for purification of total DNA from crude lysates? FAQ ID -1255 浏览 Do you have a protocol for isolation of genomic DNA from saliva and mouthwash? FAQ ID -917 浏览 Do you have a protocol for purification of total DNA from yeast? FAQ ID -1253 浏览 Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser? FAQ ID -924 浏览 Do you have a protocol for purification of total DNA from insects? FAQ ID -1254 浏览 How can I precipitate genomic DNA using isopropanol? FAQ ID -2953 浏览 What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)? FAQ ID - 3447 浏览 Do you have a protocol for the isolation of genomic DNA from sperm? FAQ ID -909 浏览 What is the composition of buffer AE? FAQ ID -730 浏览 How do I safely inactivate biohazardous flow-through material? FAQ ID -12 浏览 What is the advantage of running an analytical gel with fractions of my plasmid preparation? FAQ ID -769 浏览 What is the recipe for LB? FAQ ID -212 浏览 How do I know if my plasmid is a high- or low copy number type? FAQ ID -350 浏览 Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription? FAQ ID -366 浏览 I left Buffer P1 at room temperature after addition of RNase A, what shall I do? FAQ ID -859 浏览 How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305 浏览 With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns? FAQ ID -864 浏览 What is the recipe for 2x YT? FAQ ID -213 浏览 What are the additional plasmid bands I see on my gel? FAQ ID -1059 浏览 Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis? FAQ ID -896 浏览 What is the composition of Buffer PB? FAQ ID -2791 浏览 Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit? FAQ ID -3102 浏览 Why is my plasmid DNA yield low? FAQ ID -768 浏览 Why do I get genomic DNA contamination in my plasmid prep? FAQ ID -353 浏览 Do you have a protocol for the isolation of plasmid DNA from Agrobacterium? FAQ ID -898 浏览 I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that? FAQ ID -1045 浏览 What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent? FAQ ID -861 浏览 What is the recipe for SOC medium? FAQ ID -798 浏览 How much RNA does a typical mammalian cell contain? FAQ ID -2946 浏览 Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage? FAQ ID -1037 浏览 What are the effects of low A260/A230 ratios in RNA preparations on downstream applications? FAQ ID -2248 浏览 What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728 浏览 Do I need to purchase any consumable for processing the kit on EZ2 Connect? FAQ ID - 3849 浏览 Are we able to customize protocol on the EZ2 Connect? FAQ ID - 3850 浏览 Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit? FAQ ID - 3851 浏览 Can the kit be run on EZ1 instruments? FAQ ID - 3852 浏览 How can I check the integrity of RNA purified using RNeasy Kits? FAQ ID -1024 浏览 What are the limits of detection of the QIAxcel Connect? FAQ ID - 3853 浏览 When should the N2 bottle used with the QIAxcel Connect System be changed? FAQ ID - 3854 浏览 What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID - 3855 浏览 Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect? FAQ ID - 3856 浏览 What are the differences between QIAxcel Advanced and QIAxcel Connect? FAQ ID - 3857 浏览 Why does an intensity calibration of the gel cartridge for the QIAxcel Connect need to be performed? FAQ ID - 3858 浏览 What is the QIAxcel Connect System? FAQ ID - 3859 浏览 Can I combine your CGT dPCR assays with custom designed assays? FAQ ID - 3861 浏览 How many assays can be multiplexed? FAQ ID - 3862 浏览 In which channels can you detect the CGT dPCR assays? FAQ ID - 3863 浏览 How can I resuspend the lyophilized assay? FAQ ID - 3864 浏览 What quenchers are used? FAQ ID - 3865 浏览 Do I need to use restriction enzymes when quantifying AAV genomes? FAQ ID - 3866 浏览 Can the CGT dPCR assays also be used for the quantification of non-AAV samples? FAQ ID - 3868 浏览 Can I use the CGT dPCR assays for the titer determination of RNA viruses? FAQ ID - 3869 浏览 How do I know if the assays are compatible with the sample DNA of interest? FAQ ID - 3870 浏览 Are the assays also compatible with qPCR? FAQ ID - 3871 浏览 Can they be used with lentiviral vectors? FAQ ID - 3872 浏览 Is restriction enzyme digestion required? FAQ ID - 3875 浏览 dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter? FAQ ID - 3876 浏览 My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate? FAQ ID - 3877 浏览 Is dPCR sensitive to inhibition? FAQ ID - 3880 浏览 Is DNA extraction from the samples needed? FAQ ID - 3873 浏览 How much sample can I load for detecting host cell contamination? FAQ ID - 3879 浏览 Where can I find a copy of the .kpf file for a BioSprint protocol? FAQ ID -3073 浏览 Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730? FAQ ID -3075 浏览 What is the minimum number of samples that can be extracted on a BioSprint 96? FAQ ID -3076 浏览 Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored? FAQ ID -3077 浏览 Can I use specific primers with the FastLane Kits? FAQ ID -3078 浏览 Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C? FAQ ID -3079 浏览 Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification? FAQ ID -3080 浏览 Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect? FAQ ID - 3881 浏览 Do we have specificity and sensitivity data for this kit? FAQ ID - 3882 浏览 Do we have specificity and sensitivity data for this kit? FAQ ID - 3883 浏览 Is it possible to scale up sample volume and reaction volume? FAQ ID - 3884 浏览 What color channels are used? FAQ ID - 3885 浏览 What is the percentage of false negative results that QIAGEN obtained during validations? FAQ ID - 3886 浏览 What is the minimum and maximum concentration of effective RNA for the test? FAQ ID - 3887 浏览 Anything important when starting the cycler run? FAQ ID - 3888 浏览 Is it possible to use ABI instruments without ROX as a passive reference? FAQ ID - 3889 浏览 What is the shelf-life of the Neo Assay Kit? FAQ ID - 3890 浏览 Can the SARS-CoV-2 Neo Assay Kit detect all variants? FAQ ID - 3891 浏览 Why did we not choose commonly used genes such as the S gene, E gene, or RdRP? FAQ ID - 3892 浏览 Why did you choose ORF1a+ORF1b and ORF3a+ORF7a? FAQ ID - 3893 浏览 Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay? FAQ ID - 3894 浏览 Can I use the IC RNA of the QIAprep& Viral RNA UM Kit? FAQ ID - 3895 浏览 Which positive control should I use? FAQ ID - 3896 浏览 How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit? FAQ ID - 3897 浏览 How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature? FAQ ID - 3898 浏览 How can PCR cycling conditions be optimized for Phoenix Hot Start Taq? FAQ ID - 3899 浏览 Can Phoenix Hot Start Taq utilize cDNA as template for PCR? FAQ ID - 3900 浏览 Is Phoenix Hot Start Taq capable of multiplex PCR? FAQ ID - 3901 浏览 How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer? FAQ ID - 3902 浏览 When should I use Phoenix Hot Start Taq GC reaction Buffer? FAQ ID - 3903 浏览 What is the amplification length limit of Phoenix Hot Start Taq? FAQ ID - 3904 浏览 What is the fidelity/error rate of Phoenix Hot Start Taq? FAQ ID - 3905 浏览 Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage? FAQ ID - 3906 浏览 How can yield for long targets be increased when using Phoenix Hot Start Taq? FAQ ID - 3907 浏览 How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase? FAQ ID - 3908 浏览 How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers? FAQ ID - 3909 浏览 When should I use 5X VeraSeq GC Buffer? FAQ ID - 3910 浏览 What is the amplification length limit of VeraSeq 2.0 DNA Polymerase? FAQ ID - 3911 浏览 Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage? FAQ ID - 3913 浏览 What is the amplification length limit of VeraSeq 2.0 DNA Polymerase? FAQ ID - 3912 浏览 How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase? FAQ ID - 3914 浏览 Will VeraSeq 2.0 DNA Polymerase incorporate dUTP? FAQ ID - 3915 浏览 Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme? FAQ ID - 3916 浏览 How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling? FAQ ID - 3917 浏览 What denaturation temperature should be used in cycling conditions? FAQ ID - 3918 浏览 What annealing temperature should be used in the cycling conditions? FAQ ID - 3919 浏览 What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature? FAQ ID - 3920 浏览 How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers? FAQ ID - 3922 浏览 How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase? FAQ ID - 3921 浏览 When should I use 5X VeraSeq GC Buffer? FAQ ID - 3923 浏览 What is the amplification length limit of VeraSeq ULtra DNA Polymerase? FAQ ID - 3924 浏览 What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase? FAQ ID - 3925 浏览 How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase? FAQ ID - 3926 浏览 Will VeraSeq ULtra DNA Polymerase incorporate dUTP? FAQ ID - 3927 浏览 Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang? FAQ ID - 3928 浏览 Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme? FAQ ID - 3929 浏览 What denaturation temperature should be used in the cycling conditions? FAQ ID - 3931 浏览 What annealing temperature should be used in the cycling conditions? FAQ ID - 3932 浏览 What are the ResDNA Quant Kit components? FAQ ID - 3878 浏览 How can I load new protocols onto the QIAcube? FAQ ID -1421 浏览 Are run reports and/or log files available on the QIAcube? FAQ ID -1412 浏览 What applications are offered on the QIAcube? FAQ ID -1403 浏览 Can the QIAcube heater/shaker be used independently from protocol runs? FAQ ID -1422 浏览 What EZ1 DNA Investigator protocol is recommended for very precious samples? FAQ ID -1154 浏览 When should the Normalization protocol be used? FAQ ID -1155 浏览 What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap? FAQ ID - 3364 浏览 Does the EpiTect Hi-C Data Analysis Portal cost money? FAQ ID - 143077 浏览 What is the effect of using amounts of input material per sample that fall outside the recommended range? FAQ ID - 143064 浏览 What is the required amount of input material per sample for the EpiTect Hi-C Kit? FAQ ID - 143063 浏览 Does QIAGEN provide data analysis to accompany the EpiTect Hi-C Kit? FAQ ID - 143075 浏览 How can I extract DNA from a polyacrylamide (PAGE) gel? FAQ ID -120 浏览 Why does my DNA sample float out of the slot when loading it onto an agarose gel? FAQ ID -205 浏览 What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick? FAQ ID -148 浏览 I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost? FAQ ID -756 浏览 Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels? FAQ ID -576 浏览 Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL? FAQ ID -2032 浏览 Can the QIAcube be connected to a laboratory information management system? FAQ ID -1411 浏览 What are the dimensions and weight of the QIAcube? FAQ ID -1407 浏览 What dedicated QIAcube Kits are available? FAQ ID -2337 浏览 How can I decontaminate the QIAcube system? FAQ ID -1419 浏览 Can the QIAcube rotor and/or buckets be removed for cleaning? FAQ ID -1410 浏览 Can the QIAcube centrifuge be used independently from protocol runs? FAQ ID -1423 浏览 Do I need to buy special kits for the QIAcube? FAQ ID -1402 浏览 Do I need to discard partially used QIAcube tip racks? FAQ ID -1418 浏览 Can I program my own protocols for the QIAcube? FAQ ID -1405 浏览 Can the CGT dPCR assays also be used for the analysis of AAV genome integrity? FAQ ID - 3867 浏览 Is the QIAprep&amp CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN? 3787 浏览 Do you have a protocol for the isolation of viral RNA from stool? FAQ ID -918 浏览 Which extraction kits are recommended? FAQ ID - 3874 浏览 How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C? FAQ ID - 3934 浏览 What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)? FAQ ID - 3935 浏览 What is the amount of standard for the different kit? FAQ ID - 3936 浏览 How did you calculate the conversion factor? FAQ ID - 3937 浏览 What does the standard kit contain? FAQ ID - 3938 浏览 What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)? FAQ ID - 3939 浏览 How long are sample lysates stable in the fridge/ freezer? FAQ ID - 143765 浏览 Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument? FAQ ID -2027 浏览 Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP? FAQ ID -2031 浏览 After bisulfite conversion, can the DNA be stored? FAQ ID -2409 浏览 Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit? FAQ ID - 3940 浏览 When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit? FAQ ID - 3941 浏览 What size of RNA can be expected? FAQ ID - 3942 浏览 Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit? FAQ ID - 3947 浏览 When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit FAQ ID - 3948 浏览 Are there other options for paraffin removal? FAQ ID - 3949 浏览 What are recommended stopping points in the procedure of the EZ2 AllPrep DNA/RNA FFPE Kit? FAQ ID - 3950 浏览 Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)? FAQ ID - 3951 浏览 An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away. FAQ ID - 3952 浏览 Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue? FAQ ID - 3953 浏览 What size of RNA can be expected? FAQ ID - 3954 浏览 Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID - 3955 浏览 My overall RNA yield is fine but RT-PCR performance poor – how can I improve this? FAQ ID - 3956 浏览 Why are my DV200 values of my RNA sample so low? FAQ ID - 3957 浏览 The integrity of my DNA sample (e.g. QIAxcel Connect) is bad. FAQ ID - 3958 浏览 How do I proceed if the amount of starting material cannot be calculated in detail? FAQ ID - 3943 浏览 Are there other options for paraffin removal? FAQ ID - 3946 浏览 Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID - 3944 浏览 My overall RNA yield is fine but RT-PCR performance poor – how can I improve this? FAQ ID - 3945 浏览 Is this available without glycerol? FAQ ID - 3959 浏览 What types of priming are compatible with EnzScript M-MLV RT RNase H-? FAQ ID - 3961 浏览 What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)? FAQ ID - 3964 浏览 What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)? FAQ ID - 3960 浏览 What PCR enzymes can be used following first-strand synthesis? FAQ ID - 3963 浏览 Is the kit compatible with unpurified in-process samples? FAQ ID - 3965 浏览 Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration? FAQ ID - 3971 浏览 Do you recommend storage of highly diluted AAV samples? FAQ ID - 3972 浏览 Is it possible to store the lysates? How can the lysates be stored? FAQ ID - 3973 浏览 Can I use MspI or HpaII restriction enzymes from other suppliers? FAQ ID - 3975 浏览 How many AAV genome copies can I load into the QIAcuity? FAQ ID - 3977 浏览 Do you need to dilute the lysates before setting up the PCR mix? FAQ ID - 3978 浏览 How do I know if the CGT dPCR assays are compatible with the sample DNA of interest? FAQ ID - 3980 浏览 Are the CGT dPCR assays also compatible with qPCR? FAQ ID - 3981 浏览 Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays? FAQ ID - 3982 浏览 What is included in the SureSilencing shRNA Plasmids shipment? FAQ ID -2785 浏览 Is the QIAxcel Advanced discontinued? FAQ ID - 3983 浏览 What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit? FAQ ID - 3984 浏览 What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits? FAQ ID - 3985 浏览 What water should I use to prepare the buffer concentrates? FAQ ID - 3986 浏览 What is the new Waste Tube made of? FAQ ID - 3987 浏览 Are the QIAwave kits based on a different chemistry than the legacy kits? FAQ ID - 3990 浏览 Is there a way to compare the environmental impacts of the kit? FAQ ID - 3991 浏览 Can the QIAwave kits be recycled? FAQ ID - 3992 浏览 Does the reuse of the Waste Tubes increase the risk of cross-contamination? FAQ ID - 3988 浏览 I don’t have any glassware in the lab, is the QIAwave kit still a good option for me? FAQ ID - 3989 浏览 Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument? FAQ ID - 3993 浏览 Is the kit compatible with unpurified in-process samples? FAQ ID - 3994 浏览 My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction? FAQ ID - 3995 浏览 Can I use Internal Control as overall control? FAQ ID - 3996 浏览 How long can I store the reconstituted Internal Control and the reconstituted Positive Control? FAQ ID - 3997 浏览 Do you recommend storage of highly diluted mycoplasma samples? FAQ ID - 3998 浏览 What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit? FAQ ID - 3999 浏览 On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 浏览 Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex? FAQ ID -2569 浏览 Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System? FAQ ID -2571 浏览 Which human target is used for the quantification in the Investigator Quantiplex Kit? FAQ ID -2577 浏览 Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated? FAQ ID - 3728 浏览 Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3? FAQ ID -2570 浏览 Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit? FAQ ID - 3730 浏览 Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit? FAQ ID - 3743 浏览 Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit? FAQ ID - 3742 浏览 Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System? FAQ ID - 3732 浏览 How long does a run take? FAQ ID -2566 浏览 Investigator Quantiplex: For which real-time cyclers has the kit been validated? FAQ ID -2567 浏览 What are the storage conditions? FAQ ID - 3748 浏览 Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit? FAQ ID -2568 浏览 How can I streamline the quantification workflow? FAQ ID - 3738 浏览 Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q? FAQ ID - 3729 浏览 Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated? FAQ ID - 3741 浏览 Can I optimize a dPCR assay on the QIAcuity using gradient temperature? FAQ ID - 3783 浏览 Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits? FAQ ID -2126 浏览 Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2130 浏览 Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2131 浏览 What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761 浏览 Can I use REPLI-g for SNP Genotyping? FAQ ID -700 浏览 Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit? FAQ ID -2058 浏览 What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System? FAQ ID - 3506 浏览 Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes? FAQ ID -2595 浏览 Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays? FAQ ID -717 浏览 Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes? FAQ ID -1979 浏览 Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits? FAQ ID -1452 浏览 How is "Touchdown PCR" used to increase PCR specificity? FAQ ID -75 浏览 What should the starting template DNA quality and quantity be for PCR? FAQ ID -74 浏览 What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit? FAQ ID -165 浏览 Why do I get smeared PCR products? FAQ ID -87 浏览 Does QIAGEN sell Q-Solution separately? FAQ ID -204 浏览 Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? FAQ ID -1745 浏览 Is Q-Solution required for PCR with QIAGEN's PCR kits? FAQ ID -380 浏览下一页回到顶部联系QIAGEN 全球各分公司联系方式技术支持客户服务

What are exo-resistant random hexamers used in the REPLI-g reaction?

FAQ ID -710

The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?

FAQ ID -776

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Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?

FAQ ID -797

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What is the basic technology behind the Strep-tag Protein Purification System?

FAQ ID -740

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What are the expected average DNA yields from plant extractions on the BioSprint 96?

FAQ ID -771

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If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?

FAQ ID -718

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How to quantitate DNA purified using the BioSprint 96?

FAQ ID -773

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How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

FAQ ID -734

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Is it possible to use LiquiChip beads for FACS applications?

FAQ ID -733

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Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?

FAQ ID -763

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Is GFP excited and detected by the green laser of the LiquiChip System?

FAQ ID -737

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Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?

FAQ ID -755

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Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?

FAQ ID -758

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Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?

FAQ ID -715

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Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?

FAQ ID-751

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Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?

FAQ ID -719

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Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?

FAQ ID -703

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Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?

FAQ ID -713

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Why is there no detection of free reporter fluorescence in the LiquiChip System?

FAQ ID -732

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What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?

FAQ ID -782

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What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?

FAQ ID -779

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What is the enzyme used in the REPLI-g reaction?

FAQ ID -704

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What type of Sequencing Services does QIAGEN offer?

FAQ ID -781

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Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?

FAQ ID -766

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What is the general sensitivity level of the LiquiChip System?

FAQ ID -731

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Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?

FAQ ID -741

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Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?

FAQ ID -778

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Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?

FAQ ID -757

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Can lyophilized plant material be used for DNA extraction on the BioSprint 96?

FAQ ID -772

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Can the REPLI-g WGA enzyme work with labeled dNTPs?

FAQ ID -706

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Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?

FAQ ID -744

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Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?

FAQ ID -722

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Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?

FAQ ID -791

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Any data on the fidelity of the REPLI-g MDA technique?

FAQ ID -707

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Are Centromeres and Telomeres amplified using REPLI-g WGA?

FAQ ID -702

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Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?

FAQ ID -712

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Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?

FAQ ID -716

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How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?

FAQ ID -780

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How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?

FAQ ID -788

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How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?

FAQ ID -783

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How many cells can be used with the FastLane Cell cDNA Kit?

FAQ ID -796

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Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?

FAQ ID -746

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Can frozen blood be used for whole genome amplification with REPLI-g?

FAQ ID -794

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Do I have to switch off the laser of the LiquiChip Workstation overnight?

FAQ ID -736

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How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?

FAQ ID -738

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Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?

FAQ ID -714

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Do you synthesize thiophosphate modified random hexamers?

FAQ ID -711

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Has anyone verified whole genome amplification accuracy with Sequencing?

FAQ ID -701

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Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?

FAQ ID -793

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How best to handle the light sensitive LiquiChip reagents?

FAQ ID -735

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Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?

FAQ ID -729

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Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?

FAQ ID -397

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Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?

FAQ ID -358

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Is it possible to store TAGzyme enzymes at°C?

-80

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I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?

FAQ ID -364

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What are the calculated molecular weights of the TAGZyme enzymes?

FAQ ID -329

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How complete is the removal of DAPase in the TAGZyme process?

FAQ ID -319

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How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?

FAQ ID -392

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How do I submit a siRNA order by telephone or online?

FAQ ID -399

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How many transfections can I perform with 20 nmol of HPP grade siRNA?

FAQ ID -367

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Which primers can I use for sequencing pQE-expression vector constructs?

FAQ ID -343

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What is the standard assay volume recommended for LiquiChip assays?

FAQ ID -330

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What is the acceptable maximum g force for centrifugation of Xa Removal Resin?

FAQ ID -320

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What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?

FAQ ID -307

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What is the composition of Buffer FG3?

FAQ ID -356

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Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?

FAQ ID -354

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303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?

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Can I use a partial MinElute 96 UF plate?

FAQ ID -355

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Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?

FAQ ID -372

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Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?

FAQ ID -306

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Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?

FAQ ID -308

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Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?

FAQ ID -359

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Are QIAGEN monoclonal Anti His Antibodies glycosylated?

FAQ ID -334

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Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?

FAQ ID -332

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Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?

FAQ ID -328

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Do I need to anneal, deprotect or desalt my QIAGEN siRNA?

FAQ ID -398

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How are siRNAs introduced into C. elegans?

FAQ ID -396

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Do pQE vectors contain the CAT gene?

FAQ ID -362

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How can I improve the expression of proteins containing hydrophobic regions?

FAQ ID -339

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How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?

FAQ ID -324

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Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?

FAQ ID -323

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Can RNAprotect Tissue Reagent be used to store previously isolated RNA?

FAQ ID -337

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Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?

FAQ ID -360

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Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?

FAQ ID -312

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Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?

FAQ ID -327

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Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?

FAQ ID -318

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What data documentation does QIAGEN's Sequencing Service provide for customers?

FAQ ID -832

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What are the sequences of the FlexiTube siRNAs?

FAQ ID -851

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My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?

FAQ ID -872

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What is the proper way to turn on or turn off the EZ1 instrument?

FAQ ID -870

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What is the Energy Mix in the EasyXpress Kits?

FAQ ID -846

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What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?

FAQ ID -850

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I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?

FAQ ID -829

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Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?

FAQ ID -835

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Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?

FAQ ID -854

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Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?

FAQ ID -855

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Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?

FAQ ID -841

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Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -876

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Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?

FAQ ID -837

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Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?

FAQ ID -875

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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?

FAQ ID -878

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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?

FAQ ID -862

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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?

FAQ ID -807

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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?

FAQ ID -877

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Will the sequences of the QuantiTect Primer Assays be provided?

FAQ ID -804

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Will the FastLane Cell cDNA Kit work with suspension cells?

FAQ ID -801

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Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?

FAQ ID -836

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What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?

FAQ ID -815

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What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?

FAQ ID -873

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What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?

FAQ ID -831

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Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?

FAQ ID -806

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Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?

FAQ ID -839

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Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?

FAQ ID -808

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Where can I find QIAGEN products for a specific gene or gene product?

FAQ ID -803

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Can the QuantiTect Primer Assays be used for detection of genomic DNA?

FAQ ID -811

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Do I need to use RNase inhibitors with the RNeasy Kits?

FAQ ID -813

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Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?

FAQ ID -824

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Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?

FAQ ID -834

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Can the FastLane Cell cDNA Kit be used with tissue?

FAQ ID -821

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Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?

FAQ ID -883

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Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?

FAQ ID -881

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895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?

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Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?

-15

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Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?

FAQ ID -823

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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID -828

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Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?

FAQ ID -856

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Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?

FAQ ID -838

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Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?

FAQ ID -874

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Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?

FAQ ID -812

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Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?

FAQ ID -863

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Do you have information on in vivo RNA interference experiments?

FAQ ID -817

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Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?

FAQ ID -825

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I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?

FAQ ID -822

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How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?

FAQ ID -880

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How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?

FAQ ID -843

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Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?

FAQ ID -893

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Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?

FAQ ID -885

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Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?

FAQ ID -886

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Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?

FAQ ID -884

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Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?

FAQ ID -887

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Do you have a protocol for the isolation of plasmid DNA from yeast?

FAQ ID -853

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Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?

FAQ ID -894

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Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?

FAQ ID -890

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Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?

FAQ ID -888

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Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?

FAQ ID -889

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What are your recommendations for cotransfecting several plasmids?

FAQ ID -124

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What are the recommended culture and buffer volumes for a very low-copy plasmid?

FAQ ID -168

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Is mRNA isolation necessary for sensitive RT-PCR?

FAQ ID -111

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When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?

FAQ ID -100

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Which Anti-His Antibody is the most sensitive for my protein of interest?

FAQ ID -172

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Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?

FAQ ID -101

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What is the recommended culture medium for the QIAprep System?

FAQ ID -154

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How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?

FAQ ID -102

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How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?

FAQ ID -134

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How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?

FAQ ID -141

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How can I isolate RNA from 1 gram of plant tissue?

FAQ ID -128

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How can I improve the performance of the HiSpeed QIAprecipitator module?

FAQ ID -144

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What can I use to isolate RNA smaller than 200 nucleotides?

FAQ ID -115

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What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?

FAQ ID -170

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What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?

FAQ ID -167

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How should RNeasy Kits be stored and how long are they stable?

FAQ ID -103

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Is it possible to cleave the 6xHis-tag from an expressed protein?

FAQ ID -140

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How should QIAGEN Plasmid Purification Kits be stored and for how long?

FAQ ID -192

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What are the features and benefits of the QIAexpress 6xHis Tag System?

FAQ ID -193

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Do you have transfection data for QIAGEN Transfection Reagents?

FAQ ID -158

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Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?

FAQ ID -129

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Does QIAGEN offer vectors for direct cloning of PCR products?

FAQ ID -146

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How can I avoid variable transfection efficiencies with SuperFect Reagent?

FAQ ID -179

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Can DyeEx be used to clean up labeled DNA other than sequencing templates?

FAQ ID -137

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Are QIAamp DNA isolation kits suitable for apoptosis studies?

FAQ ID -149

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Can I use a QIAfilter Cartridge for purifying large plasmids?

FAQ ID -121

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Can I use Omniscript or Sensiscript RT's at a higher temperature?

FAQ ID -116

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How do you ensure that RNeasy buffers are RNase-free?

FAQ ID -113

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How can I purify DNA from soil, food and sewage samples for PCR?

FAQ ID -118

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How should I store Anti·His and Tag·100 Antibodies?

FAQ ID -194

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How do I prepare an insert for pQE vectors?

FAQ ID -185

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How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?

FAQ ID -143

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What are the compatibilities of different reagents with Ni-NTA matrices?

FAQ ID -49

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How can I prevent running out of M48 Kit reagents before the full number of samples are processed?

FAQ ID -1042

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Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?

FAQ ID -1019

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Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?

FAQ ID -1040

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Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?

FAQ ID -1071

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Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?

FAQ ID -1063

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Is it possible to use the PhosphoProtein Purification Kit in batch format?

FAQ ID -1069

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What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?

FAQ ID -1077

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What is the difference between TurboCapture Microplate Holders Type A and Type B?

FAQ ID -1038

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What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?

FAQ ID -1093

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Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?

FAQ ID -1049

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How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?

FAQ ID -1046

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How can I tell TurboCapture Microplate Holder Type A from Type B?

FAQ ID -1039

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How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?

FAQ ID -1086

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How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?

FAQ ID -1058

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What is the error rate of HotStar HiFidelity DNA Polymerase?

FAQ ID -1048

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Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?

FAQ ID -1092

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Which kit should I use for RNA isolation from Cartilage?

FAQ ID -1026

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Where can I find the serial number on QIAGEN's BioRobots?

FAQ ID -1064

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Which kit can be used for simultaneous purification of DNA and RNA from biopsies?

FAQ ID -1089

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Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?

FAQ ID -1044

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Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?

FAQ ID -1083

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Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?

FAQ ID -1054

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Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?

FAQ ID -1053

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Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?

FAQ ID -1084

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What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?

FAQ ID -1079

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What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?

FAQ ID -1090

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What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?

FAQ ID -1055

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What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?

FAQ ID -1034

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How can I prevent clogging of QIAfilter cartridges?

FAQ ID -1060

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What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?

FAQ ID -1067

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Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?

FAQ ID -1065

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Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?

FAQ ID -1050

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Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?

FAQ ID -1020

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Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?

FAQ ID -1007

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Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?

FAQ ID -1017

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Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?

FAQ ID -1032

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Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?

FAQ ID -1091

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Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -1078

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Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?

FAQ ID -1036

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Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?

FAQ ID -1066

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Can the AllPrep RNA/Protein Kit be used with tissue?

FAQ ID -1095

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Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?

FAQ ID -1073

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How can air bubbles in PhosphoProtein Purification Columns be avoided?

FAQ ID -1070

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How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?

FAQ ID -1062

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How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?

FAQ ID -1087

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Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?

FAQ ID - 1004

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Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?

FAQ ID -1014

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Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?

FAQ ID -1013

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Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?

FAQ ID -1012

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Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?

FAQ ID -1009

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Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?

FAQ ID -1011

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Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?

FAQ ID -1008

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Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?

FAQ ID -1010

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Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?

FAQ ID -1006

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Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?

FAQ ID - 1003

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Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?

FAQ ID -2471

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If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?

FAQ ID -266

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Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?

FAQ ID -297

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What are the main differences between other magnetic beads and the BioMag particles?

FAQ ID -272

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My BioMag is about to expire, is it still any good?

FAQ ID -268

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How magnetically responsive is BioMag?

FAQ ID -265

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If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?

FAQ ID -279

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Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.

FAQ ID -283

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What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?

FAQ ID -292

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What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?

FAQ ID -295

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What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?

FAQ ID -275

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Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?

FAQ ID -287

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Can I select FITC labeled cells with BioMag?

FAQ ID -278

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Can I use BioMag particles with any specific primary antibody?

FAQ ID -273

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Are BioMag particles stable in other solvents?

FAQ ID -267

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Can BioMag be used for positive and negative selection?

FAQ ID -269

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How can I avoid poor immunolocalization morphology with Anti-His Antibodies?

FAQ ID -200

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How can I prevent non-specific binding when using BioMag?

FAQ ID -277

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How do I prevent bubbles from forming in my Ni-NTA agarose column?

FAQ ID -285

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How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?

FAQ ID -201

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Is your Nuclease-Free Water fluorescence-free?

FAQ ID -1291

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What quality level does your Nuclease-Free Water have?

FAQ ID -1292

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How should the GelPilot DNA Loading Dye be handled?

FAQ ID -1287

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Does GelPilot DNA Loading Dye contain ethidium bromide?

FAQ ID -1286

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Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?

FAQ ID -1299

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Are MaXtract tubes siliconized?

FAQ ID -1298

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Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?

FAQ ID -1211

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What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1205

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Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?

FAQ ID -1213

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Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?

FAQ ID -1232

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Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?

FAQ ID -1217

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Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?

FAQ ID -1225

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What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?

FAQ ID -1224

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What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?

FAQ ID -1223

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What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?

FAQ ID -1208

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Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?

FAQ ID -1202

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What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?

FAQ ID -1206

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What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1201

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What is the size of the BioRobot EZ1?

FAQ ID -1245

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Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?

FAQ ID -1226

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Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?

FAQ ID -1207

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Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?

FAQ ID -1258

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Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?

FAQ ID -1216

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Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?

FAQ ID -1227

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Can RNAprotect Saliva Reagent be purchased separately?

FAQ ID -1214

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Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?

FAQ ID -1215

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Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?

FAQ ID -1204

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Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?

FAQ ID -1200

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Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?

FAQ ID -1248

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How can I decontaminate the BioRobot EZ1?

FAQ ID -1231

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How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?

FAQ ID -1212

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How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?

FAQ ID -1222

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How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?

FAQ ID -1219

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Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?

FAQ ID -1260

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Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?

-1

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How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?

FAQ ID -1203

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Which fixation method is used in the PAXgene Tissue System?

FAQ ID - 3600

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What is the fixation reagent of PAXgene Tissue FIX?

FAQ ID - 3601

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What is the stabilization reagent of PAXgene Tissue STABILIZER?

FAQ ID - 3602

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What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?

FAQ ID - 3604

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Why are two reagents used in the PAXgene Tissue System?

FAQ ID - 3603

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Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?

FAQ ID - 3605

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Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?

FAQ ID - 3606

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Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?

FAQ ID - 3607

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Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?

FAQ ID - 3608

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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?

FAQ ID - 3609

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How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3611

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Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3610

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How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3612

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Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?

FAQ ID - 3614

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What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?

FAQ ID - 3613

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Can proteins be extracted from PAXgene Tissue fixed specimens?

FAQ ID - 3616

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Does the PAXgene Tissue RNA Kit co-purify small RNAs?

FAQ ID - 3615

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Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?

FAQ ID - 3617

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What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID - 3618

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How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?

FAQ ID - 3619

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Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit?

FAQ ID - 3623

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How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?

FAQ ID - 3622

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Are QR codes recognized by QIAsymphony?

FAQ ID - 3625

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Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?

FAQ ID - 3626

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What is the reagent in the PAXgene Blood ccfDNA Tube?

FAQ ID - 3632

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Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?

FAQ ID - 3627

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What is the shelf life of unused PAXgene Blood ccfDNA Tubes?

FAQ ID - 3633

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How should unused PAXgene Blood ccfDNA Tubes be stored?

FAQ ID - 3634

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Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?

FAQ ID - 3635

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Are PAXgene Blood ccfDNA Tubes sterile?

FAQ ID - 3636

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What is the expected ccfDNA yield from plasma generated from 10 ml of blood?

FAQ ID - 3637

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Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?

FAQ ID - 3638

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How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?

FAQ ID - 3639

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Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?

FAQ ID - 3640

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Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?

FAQ ID - 3641

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Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?

FAQ ID - 3642

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Is double centrifugation required for plasma processing?

FAQ ID - 3643

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How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?

FAQ ID - 3644

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What should I do if cryoprecipitates form while thawing a frozen plasma sample?

FAQ ID - 3646

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Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?

FAQ ID - 3648

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How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?

FAQ ID - 3649

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How long after first use can a reagent cartridge be stored for further use?

FAQ ID - 3650

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For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?

FAQ ID - 3652

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Which tubes are recommended for the QIAsymphony sample rack

FAQ ID - 3653

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Which protocol line should I use to process plasma samples?

FAQ ID - 3654

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Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?

FAQ ID - 3656

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How should eluates produced with the PAXgene Blood ccfDNA System be stored?

FAQ ID - 3657

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Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?

FAQ ID - 3658

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What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?

FAQ ID - 3651

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Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?

FAQ ID - 3655

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Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?

FAQ ID - 3628

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Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?

FAQ ID - 3629

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Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?

FAQ ID - 3630

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What is the blood draw volume of the PAXgene Blood ccfDNA Tube

FAQ ID - 3631

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What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?

FAQ ID - 3685

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Where can I find the gene list for a specific RT² Profiler PCR Array?

FAQ ID - 3684

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Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?

FAQ ID - 3680

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Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?

FAQ ID - 3660

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Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?

FAQ ID - 3661

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Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?

FAQ ID - 3662

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Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?

FAQ ID - 3666

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What method is used to eliminate adapter dimers from the QIAseq miRNA library?

FAQ ID - 3667

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Does a QIAseq miRNA library include hY4 Y RNA?

FAQ ID - 3670

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What is the sequence of the QIAseq miRNA NGS 3’ Adapter?

FAQ ID - 3671

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Do the covered regions in the BED file include the primers?

FAQ ID - 3689

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What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?

FAQ ID - 3697

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Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?

FAQ ID - 3637

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Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?

FAQ ID -1191

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Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?

FAQ ID -1107

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Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?

FAQ ID -1123

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What are the applications the EasyXtal 15-Well Tool can be used for?

FAQ ID -1111

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Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?

FAQ ID -1118

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Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?

FAQ ID -1187

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What cell lines have been tested with the Fastlane Cell cDNA Kit?

FAQ ID -1175

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What does 'QIAGEN Certified Solution' for Protein Crystallization mean?

FAQ ID -1121

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How many protein crystallization conditions are available at QIAGEN?

FAQ ID -1119

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How much detection reagent do you generally recommend for LiquiChip Assays?

FAQ ID -1159

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How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?

FAQ ID -1189

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How many drops can be set up on a protein crystallization support?

FAQ ID -1110

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How many LiquiChip beads are recommended for an xMAP experiment?

FAQ ID -1161

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How much solution can I add to an EasyXtal 15-Well Tool reservoir well?

FAQ ID -1114

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In which formats are crystallization condition Screening Suites available?

FAQ ID -1127

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If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?

FAQ ID -1145

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If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?

FAQ ID -1136

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How sensitive is the LiquiChip Reader?

FAQ ID -1157

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How much time is needed for a LiquiChip assay point measurement?

FAQ ID -1162

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Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?

FAQ ID -1133

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What is the delivery time for QuantiTect Primer Assays?

FAQ ID -1142

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Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?

FAQ ID -1124

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What kind of strategy for initial screening of protein crystallization conditions do you recommend?

FAQ ID -1120

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When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?

FAQ ID -1139

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What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?

FAQ ID -1193

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What starting material can be used with the Qproteome Mitochondria Isolation Kit?

FAQ ID -1184

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Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?

FAQ ID -1153

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Are EasyXtal 15-Well Tools stackable?

FAQ ID -1116

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Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?

FAQ ID -1138

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Which QuantiTect Primer Assay should I choose for my gene of interest?

FAQ ID -1135

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Why does the QuantiTect Primer Assay for my gene of interest have only one version number?

FAQ ID -1134

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Which chemical products do you use to prepare screening solutions for protein crystallization?

FAQ ID -1122

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Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?

FAQ ID -1195

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Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?

FAQ ID -1198

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Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1199

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Why is there no QuantiTect Primer Assay for my gene of interest?

FAQ ID -1140

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What is the EasyXtal 15-Well Tool made of?

FAQ ID -1106

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What is the dynamic range of detection of the LiquiChip Reader?

FAQ ID -1158

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Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?

FAQ ID -1185

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Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?

FAQ ID -1151

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Can the EasyXtal 15-Well Tools be reused?

FAQ ID -1117

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Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells?

FAQ ID -1194

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Can your Tag antibody recognize rat MAPK1 also?

-100

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Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN?

FAQ ID -1156

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Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays?

FAQ ID -1197

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Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?

FAQ ID -1173

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Are scoring sheets available for each crystallization condition Screening Suite?

FAQ ID -1125

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Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?

FAQ ID -1188

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Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1196

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Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?

FAQ ID -1164

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Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?

FAQ ID -1146

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How are NeXtal deep-well blocks sealed? Can they be resealed?

FAQ ID -1128

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Does the EasyXtal Tool conform to an SBS format?

FAQ ID -1115

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Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?

FAQ ID -1160

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How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?

FAQ ID -1113

浏览

Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?

FAQ ID -1167

浏览

How did you determine that your AllStar Negative Control siRNA binds to RISC?

FAQ ID -1180

浏览

How is the quality of the crystallization condition Screening Suites guaranteed?

FAQ ID -1126

浏览

How do I use EasyXtal Crystallization Tools?

FAQ ID -1105

浏览

How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?

FAQ ID -1109

浏览

Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?

FAQ ID -1190

浏览

Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples?

FAQ ID -1174

浏览

Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?

FAQ ID -1186

浏览

Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports?

FAQ ID -1108

浏览

Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1?

FAQ ID -1131

浏览

Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?

FAQ ID -1165

浏览

Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?

FAQ ID -1166

浏览

Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?

FAQ ID -1168

浏览

Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?

FAQ ID -2890

浏览

Which analyses can be performed with the PyroMark Q96 ID software version 1.0?

FAQ ID -2845

浏览

Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?

FAQ ID -2887

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How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?

FAQ ID -2895

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Can I use my own siRNA design?

FAQ ID -2897

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Can I order the nucleotides from PyroMark Gold Reagents separately?

FAQ ID -2827

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Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?

FAQ ID -2896

浏览

Can I directly transfect SureSilencing shRNA Plasmids into my cells?

FAQ ID -2899

浏览

Do any of the SureSilencing shRNA Plasmids contain inducible promoters?

FAQ ID -2888

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Can PyroMark Gold reagents be vortexed?

FAQ ID -2844

浏览

What are the features of the new PyroMark Q96 ID software 2.5?

FAQ ID -2867

浏览

What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?

FAQ ID -2885

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Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?

FAQ ID -2882

浏览

What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?

FAQ ID -2889

浏览

What cell lines can I use with the SureSilencing shRNA Plasmids?

FAQ ID -2891

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How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?

FAQ ID -2893

浏览

Is the expression level of miRNA precursors lower compared to that of mature miRNAs?

FAQ ID -2807

浏览

How many times can the CDTs, NDTs, and RDTs be used?

FAQ ID -2865

浏览

What is the PyroMark Q96 Data Converter?

FAQ ID -2868

浏览

What is the pyrosequencing data exchange tool for?

FAQ ID -2864

浏览

What is the composition of Buffer APP?

FAQ ID -2804

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What is the composition of Buffer FRN?

FAQ ID -2802

浏览

What is the composition of Buffer APL?

FAQ ID -2803

浏览

Which analyses can be performed with the PyroMark Q96 MD software?

FAQ ID -2866

浏览

Which PyroMark Gold Q96 Reagent should be used for which instrument and application?

FAQ ID -2836

浏览

What is the SureSilencing shRNA Plasmids guarantee?

FAQ ID -2898

浏览

What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids?

FAQ ID -2886

浏览

Does the PyroMark CpG LINE assay target mouse transposons as well?

-1

浏览

How do I find the SureSilencing shRNA Plasmids for my gene of interest?

FAQ ID -2883

浏览

How are the SureSilencing shRNA Plasmids shipped?

FAQ ID -2884

浏览

What is the concentration of SureENTRY Transduction Reagent?

FAQ ID - 3708

浏览

Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?

FAQ ID - 3710

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Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?

FAQ ID - 3712

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What sample tubes can I use on my QIAsymphony SP system?

FAQ ID - 3713

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How can I decontaminate my QIAsymphony SP / AS system?

FAQ ID - 3714

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Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?

FAQ ID - 3717

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How is the Pyromark Q48 cartridge to be cleaned?

FAQ ID - 3718

浏览

Can the disc and absorber strips for the Pyromark Q48 be reused?

FAQ ID - 3720

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What is the dead volume of the cartridges in PyroMark Q48?

FAQ ID - 3719

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How much DNA is needed in the PyroMark Q48?

FAQ ID - 3721

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Can I use PyroMark Q48 for diagnostics?

FAQ ID - 3722

浏览

Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?

FAQ ID - 3723

浏览

Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?

FAQ ID - 3724

浏览

Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?

FAQ ID - 3725

浏览

Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?

FAQ ID - 3726

浏览

Does QIAGEN offer an anti-GFP gapmer control?

FAQ ID - 3739

浏览

Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?

FAQ ID - 3745

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How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?

FAQ ID - 3744

浏览

Do I need to buy special kits for the QIAcube Connect?

FAQ ID - 141485

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Can I program my own protocols for the QIAcube Connect?

FAQ ID - 141486

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Can the QIAcube Connect rotor and/or buckets be removed for cleaning?

FAQ ID - 141491

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Can purification columns from other suppliers be processed on the QIAcube Connect?

FAQ ID - 141487

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How can I get software updates for the QIAcube Connect?

FAQ ID - 141494

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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?

FAQ ID - 141496

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Do I need to discard partially used QIAcube Connect tip racks?

FAQ ID - 141499

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How can I decontaminate the QIAcube Connect system?

FAQ ID - 141500

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Can the QIAcube Connect heater/shaker be used independently from protocol runs?

FAQ ID - 141502

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How can I load new protocols onto the QIAcube Connect?

FAQ ID - 141501

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What dedicated QIAcube Connect kits are available?

FAQ ID - 141504

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Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?

FAQ ID - 1414507

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Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?

FAQ ID - 141505

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Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?

FAQ ID - 141506

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Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?

FAQ ID - 141508

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How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?

FAQ ID - 141509

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Can the CB and RB sample tubes be used interchangeably?

FAQ ID - 141510

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Which network settings are supported by the QIAcube Connect and Q-Base?

FAQ ID - 141517

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Why can I not find the Q-Base device in the device list during the first installation?

FAQ ID - 141516

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Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?

FAQ ID - 1414518

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Why is the QIAcube Connect App reacting so slowly?

FAQ ID - 141519

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Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?

FAQ ID - 1414520

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Why does the connection test fail?

FAQ ID - 141522

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How can I accurately determine the amount of input material for the EpiTect Hi-C Kit?

FAQ ID - 143065

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What sample source do you support?

FAQ ID - 143066

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How long does it take to complete the EpiTect Hi-C protocol?

FAQ ID - 143068

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How many reactions are in a kit?

FAQ ID - 143067

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Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?

FAQ ID - 143069

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Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?

FAQ ID - 143070

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Are there stopping points in the Hi-C workflow?

FAQ ID - 143071

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With which platform can I perform my sequencing?

FAQ ID - 143072

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With which read length should the EpiTect Hi-C NGS libraries be sequenced

FAQ ID - 143073

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How do I know if my Hi-C sample is a good candidate for deep sequencing?

FAQ ID - 143074

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What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform?

FAQ ID - 143076

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Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments?

FAQ ID - 143078

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How critical is the time of the temperature change from 60°C to 80°C?

FAQ ID - 143768

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Is it possible to use Lyse&Spin baskets?

FAQ ID - 143762

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Why is DTT used only for semen samples?

FAQ ID - 143761

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Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?

FAQ ID - 143079

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Can the lysis volume be reduced for higher sensitivity?

FAQ ID - 143763

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What if only one heater shaker is available?

FAQ ID - 143764

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Why is there no male DNA obtained from sexual assault samples?

FAQ ID - 143767

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If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)

FAQ ID - 143766

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Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?

FAQ ID -147395

浏览

What RNAs has QIAseq FastSelect been designed to remove?

FAQ ID -147910

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How does QIAseq FastSelect work?

FAQ ID -147907

浏览

Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?

FAQ ID -147909

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Will QIAseq FastSelect work in other species?

FAQ ID -147911

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What library prep kits has QIAseq FastSelect been tested with?

FAQ ID -147912

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I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?

FAQ ID -147913

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What RNA range has the QIAseq FastSelect been tested with?

FAQ ID -147914

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Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?

FAQ ID -147915

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Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?

FAQ ID -147916

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Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?

FAQ ID -1

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How should I quantify RNA isolated with RNeasy Kits?

FAQ ID -32

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When is chloramphenicol amplification of plasmids performed?

FAQ ID -3

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What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?

FAQ ID -45

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What are the most commonly used protease inhibitors?

FAQ ID -53

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How should I propagate pQE expression plasmids?

FAQ ID -58

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How can I increase the amount of soluble recombinant protein in E. coli expression?

FAQ ID -64

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How can I remove imidazole from a protein sample?

FAQ ID -91

浏览

Why does my isolated RNA have a low OD 260/280 ratio?

FAQ ID -97

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How can I avoid little or no RNA yields when using an RNeasy Kit?

FAQ ID -28

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How should I store plant material for DNA isolation using the DNeasy Plant Kit?

FAQ ID -114

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Do I need to use an RNase inhibitor in my RT reaction?

FAQ ID -119

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Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?

FAQ ID -127

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Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?

FAQ ID -130

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How does imidazole affect my quantitation of protein?

FAQ ID -132

浏览

Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?

FAQ ID -133

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What is the difference between disruption and homogenization in the RNeasy System?

FAQ ID -139

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What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?

FAQ ID -142

浏览

Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?

FAQ ID -147

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What is the genotype of the EZ Competent Cells?

FAQ ID -157

浏览

What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?

FAQ ID -160

浏览

What are the molecular weights of proteins in the 6xHis Protein Ladder?

FAQ ID -169

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How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?

FAQ ID -176

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How can I improve recoveries when using the QIAquick Kits?

FAQ ID -180

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How can I improve transfection efficiency using Effectene Transfection Reagent?

FAQ ID -181

浏览

What is the principle behind Effectene Transfection Reagent?

FAQ ID -184

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What is the composition of Buffer EB?

FAQ ID -199

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Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?

FAQ ID - 3709

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Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?

FAQ ID - 3711

浏览

What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?

FAQ ID - 3715

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Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?

FAQ ID - 3716

浏览

How can I check for purity of RNA isolated using RNeasy Kits?

FAQ ID -1023

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What is the recipe for 1x PBS solution?

FAQ ID -1030

浏览

Where can I find a protocol for cleanup of already purified plasmid DNA?

FAQ ID -1031

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How do I perform an Acetone Precipitation for concentrating and desalting protein samples?

FAQ ID -1035

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What is the difference between Buffers RLT and RLT Plus?

FAQ ID -1043

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What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?

FAQ ID -1047

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Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?

FAQ ID -1051

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Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?

FAQ ID -1052

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How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?

FAQ ID -1061

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What should I use as a standard for absolute quantification in real-time PCR?

FAQ ID -1085

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What does transcript variant mean when searching for specific QuantiTect Primer Assays?

FAQ ID -1137

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What is a QuantiTect Primer Assay?

FAQ ID -1141

浏览

Do QuantiTect Primer Assays contain SYBR Green dye?

FAQ ID -1143

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What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?

FAQ ID -1218

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What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?

FAQ ID -1220

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What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?

FAQ ID -1221

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Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?

FAQ ID -1250

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Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?

FAQ ID -1251

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What is the pH value of QIAGEN's Nuclease-Free Water?

FAQ ID -1290

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Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?

FAQ ID -1327

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Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?

FAQ ID -1328

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Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?

FAQ ID -1352

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How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?

FAQ ID -1354

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What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?

FAQ ID -1356

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How do I resuspend my FlexiPlate siRNAs?

FAQ ID -1360

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Are there any specific cleaning solutions recommended for the QIAcube?

FAQ ID -1416

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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?

FAQ ID -1450

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Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1577

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What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1580

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What is the binding capacity of the Ni-NTA Superflow Cartridges?

FAQ ID -1603

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How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?

FAQ ID -1644

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Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?

FAQ ID -1659

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Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?

FAQ ID -1664

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What is the composition of Buffer PE?

FAQ ID -1691

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What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?

FAQ ID -1738

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How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?

FAQ ID -1838

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Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?

FAQ ID -1848

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What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID -1852

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If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

FAQ ID -1858

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What are the dimensions and weight of the QIAsymphony SP System?

FAQ ID -1901

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Are there any specific cleaning solutions recommended for the QIAsymphony SP?

FAQ ID -1913

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How much sample can be used with the RNeasy Plus 96 Kit?

FAQ ID -1992

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What is the composition of Buffer P2?

FAQ ID -203

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A white precipitate has formed in my 10x RT buffer. Is it still ok to use?

FAQ ID -216

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For how long are BioMag Particles stable?

FAQ ID -262

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I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?

FAQ ID -263

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Can I clean up my DNase treated RNA samples using RNeasy columns?

FAQ ID -286

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How can one determine the optimal annealing temperature for a specific PCR assay?

FAQ ID -288

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What is the composition of the QIAGEN Multiplex PCR Buffer?

FAQ ID -289

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Can I use HEPES buffer instead of phosphate in my Ni-NTA column?

FAQ ID -291

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Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?

FAQ ID -293

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What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?

FAQ ID -2004

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What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

FAQ ID -2029

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How can a C-terminal 6xHis-tagged QIAgene be expressed?

FAQ ID -2041

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Why is there more than one QIAgene for my gene of interest?

FAQ ID -2044

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Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

FAQ ID -2079

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Does the Rotor-Gene Q software have the capacity to export data?

FAQ ID -2089

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Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?

FAQ ID -2122

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Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2163

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Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?

FAQ ID -2190

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How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?

FAQ ID -2192

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Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?

FAQ ID -2196

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Why do I need normalization using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2198

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What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2203

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What buffer conditions give the best resolution for agarose gel electrophoresis?

FAQ ID -2256

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How much DNA should be loaded per well of an agarose gel?

FAQ ID -2259

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Why do I have wavy DNA bands on my agarose gel?

FAQ ID -2260

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What is the "seed sequence" of a miRNA?

FAQ ID -2262

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Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?

FAQ ID -2301

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Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?

FAQ ID -2315

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How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?

FAQ ID -2340

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How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?

FAQ ID -2341

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How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?

FAQ ID -2350

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Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?

FAQ ID -2371

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Can I buy QIAquick and MinElute columns separately?

FAQ ID -2460

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What is the PAXgene 96 filter plate?

FAQ ID -2486

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Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?

FAQ ID -2509

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Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?

FAQ ID -2514

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How long is the fixation time?

FAQ ID -2519

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What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?

FAQ ID -2543

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Are there any accessories required for sample disruption with the TissueLyser LT?

FAQ ID -2551

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Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?

FAQ ID -2603

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During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit

FAQ ID -2607

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What is the SBTengine® software?

FAQ ID -2628

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How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?

FAQ ID -2654

浏览

What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?

FAQ ID -2659

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How can I avoid or remove genomic DNA contamination from the total RNA preparation?

FAQ ID -2662

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What are the differences between one-step and two-step RT--PCR?

FAQ ID -2666

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What is qPCR?

FAQ ID -2668

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What are the common primer and probe chemistries utilized for qPCR assays?

FAQ ID -2669

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What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?

FAQ ID -2671

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What negative controls are typically included in qPCR and/or qRT-PCR experiments?

FAQ ID -2672

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What positive controls are typically included in qPCR and/or qRT-PCR experiments?

FAQ ID -2673

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What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?

FAQ ID -2674

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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?

FAQ ID -2675

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How does HotStart PCR help minimize nonspecific amplification events?

FAQ ID -2676

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What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?

FAQ ID -2678

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What is the delta Rn value?

FAQ ID -2681

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What is the threshold cycle or Ct value?

FAQ ID -2682

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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?

FAQ ID -2684

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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?

FAQ ID -2685

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Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?

FAQ ID -2686

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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?

FAQ ID -2689

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Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?

FAQ ID -2690

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What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?

FAQ ID -2691

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What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?

FAQ ID -2692

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What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?

FAQ ID -2693

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How do I determine the amplification efficiency of my qPCR assay?

FAQ ID -2694

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How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?

FAQ ID -2696

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What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?

FAQ ID -2705

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What do I need to complete a RT² qPCR Primer Assay?

FAQ ID -2707

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How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?

FAQ ID -2729

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What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?

FAQ ID -2739

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How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?

FAQ ID -2749

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How can I identify the binding sites of my transcription factor using ChIP?

FAQ ID -2753

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At what point can I stop and freeze my samples when using ChIP?

FAQ ID -2754

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What is RNA interference (RNAi)?

FAQ ID -2755

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What are the key factors for success in an RNAi experiment?

FAQ ID -2758

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What criteria should one use in choosing between siRNA versus shRNA for their studies?

FAQ ID -2771

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How does stable transfection of a shRNA-encoding plasmid work?

FAQ ID -2779

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What are the SureSilencing shRNA Plasmids?

FAQ ID -2784

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What is the composition of Buffer S3?

FAQ ID -2788

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What is the composition of Buffer ETR?

FAQ ID -2789

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What is the composition of Buffer BB?

FAQ ID -2790

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What is the composition of Buffer RLT plus?

FAQ ID -2794

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What is the composition of Buffer RLC?

FAQ ID -2795

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What is the composition of Buffer RPE?

FAQ ID -2797

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What is the composition of Buffer RWT?

FAQ ID -2798

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What is the composition of gDNA Eliminator Solution?

FAQ ID -2799

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What is the composition of Buffer RDD?

FAQ ID -2800

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What is the composition of Buffer PKD?

FAQ ID -2801

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What is the composition of Buffer ALO?

FAQ ID -2805

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What is the composition of RBC Lysis Solution?

FAQ ID -2808

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What is the composition of Cell Lysis Solution?

FAQ ID -2809

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What is the composition of Protein Precipitation Solution?

FAQ ID -2810

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What is the composition of Cell Suspension Solution?

FAQ ID -2811

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What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?

FAQ ID -2812

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What is the composition of DNA Hydration Solution in Gentra Puregene Kits?

FAQ ID -2813

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What is the meaning of the abbreviations NDT and CDT and what are the differences?

FAQ ID -2841

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What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?

FAQ ID -2849

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Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?

FAQ ID -2894

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Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

FAQ ID -2926

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What is the composition of Buffer G2?

FAQ ID -2943

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What is the composition of Buffer B1?

FAQ ID -2944

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What is the composition of Buffer B2?

FAQ ID -2945

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How much DNA does a human cell contain?

FAQ ID -2948

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How much RNA does a bacterial cell contain?

FAQ ID -2949

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How much DNA and RNA can be expected from human blood cells?

FAQ ID -2950

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What is the cellular composition of human blood?

FAQ ID -2951

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How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?

FAQ ID -2954

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Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?

FAQ ID -2980

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Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?

FAQ ID -2987

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What is the sequence of the miScript Universal Primer?

FAQ ID -2994

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How can I keep my centrifuge tubes endotoxin-free?

FAQ ID -301

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Can Buffers N3 and P3 be used interchangeably?

FAQ ID -310

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Are QIAprep and QIAquick Spin columns interchangeable?

FAQ ID -311

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Can I store agarose gel slices containing DNA for gel extraction at a later point?

FAQ ID -313

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How can QIAGEN Protease and Proteinase K be inactivated?

FAQ ID -315

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Does the 5x OneStep RT-PCR Buffer contain BSA?

FAQ ID -326

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What is the origin of replication and the plasmid copy number of the pQE vectors?

FAQ ID -338

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Is QIAGEN carrier RNA sold separately?

FAQ ID -351

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What is the white insoluble precipitate in my resuspended plasmid DNA pellet?

FAQ ID -352

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What is the composition of PBS?

FAQ ID -361

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How can I express toxic protein in E. coli?

FAQ ID -373

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How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

FAQ ID -374

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What are the dissociation constants for the Anti-His Antibodies?

FAQ ID -385

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What is the average molecular weight of a siRNA, and how do I convert uM to ug values?

FAQ ID -388

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Can siRNA silence bacterial genes?

FAQ ID -395

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How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?

FAQ ID -3003

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Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?

FAQ ID -3032

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Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?

FAQ ID -3053

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Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?

FAQ ID -3054

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What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?

FAQ ID -3055

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What is the thermal output for a BioRobot MDx and BioRobot Universal?

FAQ ID -3056

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What is the thermal output for a QIAsymphony SP and AS instrument?

FAQ ID -3057

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How precise is the QIAgility?

FAQ ID -3063

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What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?

FAQ ID -3064

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How long does it take to set up a 96-well plate PCR reaction on the QIAgility?

FAQ ID -3066

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Can I use a bar code scanner or reader on the QIAgility?

FAQ ID -3067

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How can I find the run parameters for a BioSprint protocol that I am running?

FAQ ID -3069

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Can BioSprint 96 be connected to LIMS?

FAQ ID -3070

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What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?

FAQ ID -3081

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What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?

FAQ ID -3082

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What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?

FAQ ID -3083

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What is the concentration of the different Proteinase K solutions sold by QIAGEN?

FAQ ID -3084

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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?

FAQ ID -3085

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What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?

FAQ ID -3086

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How to upload a Flexiplate on QIAGEN Webpage?

FAQ ID -3103

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Can we do modification XXXXX for custom siRNA that is not on the web page?

FAQ ID -3107

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What is the stability of siRNA lyophilized and resuspended?

FAQ ID -3109

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Do you offer FlexiTube or FlexiPlate siRNAs for rat?

FAQ ID -3114

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Is it possible to extract RNA from frozen blood?

FAQ ID -3119

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Is it possible to extract RNA from RNAprotect-stabilized-blood?

FAQ ID -3120

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What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails

-48

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Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?

FAQ ID -3125

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Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?

FAQ ID -3132

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How do I remove the RNA contamination from purified DNA?

FAQ ID -3133

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What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?

FAQ ID -3140

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What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?

FAQ ID -3169

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What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?

FAQ ID -3174

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What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?

FAQ ID -3175

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What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?

FAQ ID -3178

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Is REST software Windows 7 compatible?

FAQ ID -3182

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Can the Rotor-Gene Q perform gradient PCR?

FAQ ID -3183

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What is the expected amplicon size of the Microbial DNA qPCR Assays?

FAQ ID — 3396

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What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?

FAQ ID — 3397

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What is LLOQ?

FAQ ID — 3401

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What is the difference between LLOQ and LOD?

FAQ ID — 3402

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What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?

FAQ ID - 3427

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What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?

FAQ ID - 3429

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Can the miScript II RT Kit be used to quantify plant miRNAs?

FAQ ID - 3431

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What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?

FAQ ID - 3444

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Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?

FAQ ID - 3467

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What is the proper disposal procedure for waste from the sample preparation?

FAQ ID - 3470

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What is the expected yield from 8.5 ml of blood?

FAQ ID - 3496

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What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?

FAQ ID - 3501

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How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

FAQ ID - 3507

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How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

FAQ ID - 3508

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What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?

FAQ ID - 3510

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Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?

FAQ ID - 3512

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What does PAXgene Tissue STABILIZER contain?

FAQ ID - 3513

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What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?

FAQ ID - 3516

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Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?

FAQ ID - 3517

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What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?

FAQ ID - 3519

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Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?

FAQ ID - 3521

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Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?

FAQ ID - 3534

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Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?

FAQ ID - 3559

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Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?

FAQ ID - 3573

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Is clot detection possible on the QIAsymphony?

FAQ - ID 3588

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How can I determine if a tube is suitable for clot detection on QIAsymphony?

FAQ ID - 3590

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How should I thaw frozen plasma samples?

FAQ ID - 3645

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How do Unique Molecular Indexes (UMIs) improve quantification?

FAQ ID - 3669

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Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?

FAQ ID - 3686

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I would like to stop during the library preparation procedure. When can I do that?

FAQ ID - 3687

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Which regions are covered by the GR DNAseq V2 Panels (#181900)?

FAQ ID - 3688

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When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?

FAQ ID - 3690

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How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?

FAQ ID - 3691

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Are peak heights of Q48 comparable to other PyroMark instruments?

FAQ ID - 3692

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How much space does the PyroMark Q48 Autoprep instrument need?

FAQ ID - 3693

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Can unused wells in a pyrosequencing discs be used in the next run?

FAQ ID - 3694

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Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?

FAQ ID - 3695

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What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?

FAQ ID - 3696

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What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?

FAQ ID - 3698

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How do I prepare Buffer MP?

FAQ ID - 3620

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Are the QIAshredder columns included in various QIAGEN kits the same?

FAQ ID - 3624

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What is the composition of buffer QBT?

FAQ ID -411

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What is the composition of buffer QC?

FAQ ID -412

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What is the composition of buffer QF?

FAQ ID -413

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What is the composition of buffer STE?

FAQ ID -415

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What is the composition of buffer TE?

FAQ ID -416

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What is the composition of buffer FWB2?

FAQ ID -417

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What is the composition of buffer P3?

FAQ ID -418

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What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?

FAQ ID -470

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Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?

FAQ ID -473

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Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?

FAQ ID -474

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Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?

FAQ ID -482

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Can Ni-NTA resins be used to purify protein with an internal His-tag?

FAQ ID -496

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How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?

FAQ ID -498

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Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?

FAQ ID -500

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Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?

FAQ ID -518

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What is the composition of the siRNA resuspension & annealing buffer?

FAQ ID -522

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Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?

FAQ ID -523

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What is Tissue-Tek O.C.T., and what is it used for?

FAQ ID -530

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Why do replicates in real-time PCR have different plateau heights?

FAQ ID -539

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How can I avoid primer-dimer formation during PCR amplification?

FAQ ID -544

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How can I tell if I have primer-dimers in my PCR reaction?

FAQ ID -552

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How should I store cDNA produced using Omniscript Reverse Transcriptase?

FAQ ID -561

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Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?

FAQ ID -563

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What is the composition of Buffer ER? Is it available separately?

FAQ ID -571

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What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?

FAQ ID -572

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Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?

FAQ ID -575

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Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?

FAQ ID -577

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What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?

FAQ ID -600

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Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?

FAQ ID -603

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What do you recommend for the cleanup of genomic DNA (gDNA)?

FAQ ID -618

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How can I improve my RNA yield from liver sample when using RNeasy Kits?

FAQ ID -623

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Why do you recommend using Triton X for the purification of 6xHis-tagged protein?

-100

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What 96-well plates do you recommend for use with the LiquiChip Workstation?

FAQ ID -630

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What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?

FAQ ID -631

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Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?

FAQ ID -633

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Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?

FAQ ID -637

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What is the shelf-life of my QIAGEN Kit?

FAQ ID -651

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What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?

FAQ ID -665

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Can I purchase Phi29 DNA polymerase only?

FAQ ID -708

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What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?

FAQ ID -721

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I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?

-20

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I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?

FAQ ID -745

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How much DNA is obtained in the average PCR reaction?

FAQ ID -750

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Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?

FAQ ID -754

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Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?

FAQ ID -759

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How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?

FAQ ID -762

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What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?

FAQ ID -764

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What is the composition of Buffer N3?

FAQ ID -767

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Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?

FAQ ID -770

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How should fluorescent labeled probes be stored?

FAQ ID -784

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Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?

FAQ ID -785

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Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?

FAQ ID -786

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How can I get specific information and a quote for my individual Sequencing Service needs?

FAQ ID -792

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Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?

FAQ ID -795

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How can I separate PCR fragments that are small and very close in size on an agarose gel?

FAQ ID -800

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Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?

FAQ ID -802

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What Disinfectant Solution do you recommend for the BioRobot MDx?

FAQ ID -809

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Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?

FAQ ID -842

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What annealing temperature should be used with the QuantiTect Primer Assays?

FAQ ID -849

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Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?

FAQ ID -860

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Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?

FAQ ID -865

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Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?

FAQ ID -879

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Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

FAQ ID -900. Test

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Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?

FAQ ID -907

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Do you have a protocol for the isolation of genomic DNA from bone?

FAQ ID -908

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What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?

FAQ ID -942

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Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?

FAQ ID -959

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Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?

FAQ ID -971

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Do you have a protocol for the purification of PCR products using the BioSprint System?

FAQ ID -984

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Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

FAQ ID -997

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How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?

FAQ ID - 3707

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How can I increase expression of my 6xHis-tagged protein in E. coli?

FAQ ID -63

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Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?

148723

浏览

What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?

148724

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What is the composition of buffer OW2?

FAQ ID -419

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What is the composition of buffer OBB?

FAQ ID -421

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Do I need to buy special kits for the QIAcube Connect MDx?

148725

浏览

What applications are offered on the QIAcube Connect MDx instrument?

148726

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Can I program my own protocols for the QIAcube Connect MDx?

148727

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Can purification columns from other suppliers be processed on the QIAcube Connect MDx?

148728

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What are the dimensions and weight of the QIAcube Connect MDx?

148729

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Can the QIAcube rotor adapters be autoclaved?

148730

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Can the QIAcube rotor and/or buckets be removed for cleaning?

148731

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Can the QIAcube be connected to a laboratory information management system?

148732

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Are run reports and/or log files available on the QIAcube?

148733

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How can I get software updates for the QIAcube Connect MDx?

148734

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Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion?

148735

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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

148736

浏览

Do I need to discard partially used QIAcube tip racks?

148738

浏览

How can I decontaminate the QIAcube Connect MDx system?

148739

浏览

How can I load new protocols onto the QIAcube Connect MDx?

148740

浏览

Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs?

148741

浏览

Can the QIAcube Connect MDx centrifuge be used independently from protocol runs?

148742

浏览

What dedicated QIAcube Kits are available?

148743

浏览

Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?

148744

浏览

Can I run life science and custom protocols on a QIAcube Connect MDx?

148745

浏览

What are the heating/cooling rates in the QIAquant instruments?

153919

浏览

Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results?

153920

浏览

What sample volume is suitable for use in the QIAquant?

153921

浏览

What types of reaction vessels are required for use in the QIAquant?

153922

浏览

Is the QIAquant equipped with a temperature gradient function?

153923

浏览

Are the 96- and 384-sample blocks interchangeable?

153924

浏览

Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel?

153925

浏览

How many molecules can be detected in the system?

153926

浏览

What applications can the QIAquant be used for?

153927

浏览

Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels?

153928

浏览

Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument?

153929

浏览

Is the software of the QIAquant compatible with 21CFR part11?

153930

浏览

Is it possible to import a standard curve from a previous run?

153947

浏览

Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?

153948

浏览

Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384?

153950

浏览

Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?

153951

浏览

Is it possible to establish an internet connection with QIAquant instrument?

153952

浏览

Can I connect more than one QIAquant instrument to the same computer?

153953

浏览

What is the algorithm behind data normalization in the QIAquant Software?

153954

浏览

Is it possible to edit the sample sheet after the run in the QIAquant Software?

153955

浏览

What are the LIMS capacity of the software?

153949

浏览

Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export?

153946

浏览

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

FAQ ID - 3560

浏览

Where can I find ScreenGel profiles for the Large Fragment Marker Kit?

154189

浏览

Do I need to dilute the size marker?

154191

浏览

Can I start a QIAxcel run without using a process profile?

154188

浏览

There is n/a as peak assignment instead of the size of my fragments. How can I solve this?

154190

浏览

Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing?

153891

浏览

153892

浏览

Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure?

153893

浏览

Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C?

153894

浏览

Can the 2nd Prot K step be omitted?

153896

浏览

What size of DNA can be expected?

153897

浏览

What is the difference of buffer FTB versus Buffer ATL?

153895

浏览

Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?

3761

浏览

How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite?

3762

浏览

Can I see error codes on the instrument touchscreen?

3763

浏览

How are results stored within the QIAcuity Software Suite?

3764

浏览

What is the scope of a regular maintenance of the QIAcuity?

3765

浏览

Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex?

3766

浏览

Can I use an empty nanoplate without seal to perform a dry run?

3767

浏览

Does an incorrect handling of a plate might affect the results?

3768

浏览

What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?

3769

浏览

Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?

3770

浏览

Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?

3771

浏览

How can I get the latest VPF (Volume Precision Factor)?

3772

浏览

Where can I find assays specifically designed for QIAcuity?

3773

浏览

Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?

3774

浏览

Can I use the dPCR master mix in a qPCR cycler for optimization purposes?

3775

浏览

What is the purpose of the reference channel in QIAcuity?

3776

浏览

How to prepare DNA prior to dPCR?

3778

浏览

Can I use a custom master mix instead of a QIAGEN master mix?

3777

浏览

Which sample preparation method is recommended for QIAcuity dPCR?

3779

浏览

What are the storage conditions and expiry date of QIAcuity consumables?

3780

浏览

What is the input volume for QIAcuity Nanoplate well?

3782

浏览

Can I use the QIAcuity Nanoplate in more than one runs?

3781

浏览

What is the VPF (Volume Precision Factor)?

3784

浏览

When and how often do I need a new VPF (Volume Precision Factor)?

3785

浏览

Is a standard curve needed in dPCR?

3786

浏览

Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?

3788

浏览

Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine?

3791

浏览

Is there something to consider when working with transduced cells treated with Polybrene?

3790

浏览

For sequencing application, does the lysate require purification?

3792

浏览

Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?

3793

浏览

Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?

3794

浏览

Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?

3795

浏览

What is the minimum cell number needed for cell lysis?

3789

浏览

For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?

3796

浏览

What is the sequence of the QIAseq miRNA NGS 5’ Adapter?

FAQ ID - 3672

浏览

What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits?

3753

浏览

Are there other options for paraffin removal?

3754

浏览

Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?

3755

浏览

Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C?

3756

浏览

What is the difference between Buffer FTB and Buffer ATL?

3757

浏览

Can the 2nd Proteinase K step be omitted?

3758

浏览

What size of DNA can be expected?

3759

浏览

Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing?

3760

浏览

Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?

FAQ ID -1056

浏览

3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?

FAQ ID - 3323

浏览

Does salt concentration in the food matter when using the DNeasy mericon Food Kit?

FAQ ID - 3346

浏览

Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?

FAQ ID - 3347

浏览

When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?

FAQ ID - 3348

浏览

Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?

FAQ ID - 3349

浏览

CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?

FAQ ID - 3350

浏览

What is the composition of QIAzol? What is the color of this reagent?

FAQ ID - 3355

浏览

Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?

FAQ ID - 3362

浏览

I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?

FAQ ID - 3363

浏览

What is the QIAxcel QX RNA Size Marker 200-6000nt?

FAQ ID - 3365

浏览

What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?

FAQ ID - 3366

浏览

Do you sell CoralLoad dye separately?

FAQ ID - 3368

浏览

Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?

FAQ ID - 3369

浏览

How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at 2–8°C?

FAQ ID - 3379

浏览

Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)?

FAQ ID - 3384

浏览

What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?

FAQ ID - 3387

浏览

Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?

FAQ ID - 3388

浏览

Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?

FAQ ID - 3389

浏览

Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?

FAQ ID - 3390

浏览

Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?

FAQ ID - 3391

浏览

Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?

FAQ ID - 3392

浏览

What is the RNase A concentration and composition of Buffer P1?

FAQ ID -198

浏览

Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?

3797

浏览

Where can I download the protocol for the KingFisher Flex?

3798

浏览

Who can help in case of issues with the third party instrument?

3799

浏览

Is the virus inactivated during the procedure?

3800

浏览

Can I use sample volumes other than 300 µl?

3801

浏览

Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?

3802

浏览

I would like to use the kit on another third party instrument. Who should I contact?

3803

浏览

How long does a saliva collection take?

3804

浏览

What can I do to stimulate saliva flow?

3805

浏览

Can I stop saliva collection before the fill line is reached?

3806

浏览

What can I do if I don't see the fill line anymore due to saliva bubbles?

3807

浏览

What happens if I accidentally unscrew the funnel before saliva collection is completed?

3808

浏览

May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely?

3809

浏览

What is in the additive in the PAXgene Saliva Collector?

3810

浏览

What is the shelf life of unused PAXgene Saliva Collectors?

3811

浏览

How should unused PAXgene Saliva Collector be stored?

3812

浏览

What should I avoid before sample collection?

3813

浏览

How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva?

3814

浏览

How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature?

3815

浏览

Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C?

3816

浏览

Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C?

3817

浏览

Is RNA preserved in saliva collected in PAXgene Saliva Collector?

3818

浏览

Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?

3819

浏览

What should I do if stabilized saliva samples are very heterogenous?

3820

浏览

What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector?

3823

浏览

Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument?

3824

浏览

Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation?

3826

浏览

Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?

3827

浏览

Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?

3828

浏览

Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity?

3829

浏览

Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack?

3825

浏览

What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector?

3822

浏览

Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?

3821

浏览

What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments?

FAQ ID - 3837

浏览

Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?

FAQ ID - 3836

浏览

What is the sequence of the UDI 5' Adapter?

FAQ ID - 3835

浏览

Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit?

FAQ ID - 3674

浏览

What can be used to validate results obtained with the QIAseq miRNA Library Kit?

FAQ ID - 3675

浏览

What is the maximum number of available sample indexes?

FAQ ID - 3665

浏览

What are recommended stopping points during the QIAseq miRNA Library Kit procedure?

FAQ ID - 3663

浏览

Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?

FAQ ID - 3659

浏览

What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?

FAQ ID - 3668

浏览

What is the acceptable number of reads per sample per miRNA sequencing run?

FAQ ID - 3673

浏览

Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?

FAQ ID -1169

浏览

Is training of lab personnel included with the purchase of a BioRobot EZ1?

FAQ ID -1239

浏览

Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

FAQ ID -1240

浏览

How can we get our BioRobot EZ1 upgraded when new protocols are launched?

FAQ ID -1241

浏览

Where can I find information about the worktable setup on the BioRobot EZ1?

FAQ ID -1243

浏览

What disposables are required for nucleic acid isolations on the BioRobot EZ1?

FAQ ID -1246

浏览

How should I prepare buffy coat samples for use on the BioRobot EZ1?

FAQ ID -1249

浏览

Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?

FAQ ID -985

浏览

What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

FAQ ID -528

浏览

Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?

FAQ ID -975

浏览

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?

FAQ ID -976

浏览

Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?

FAQ ID -992

浏览

Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?

FAQ ID -1025

浏览

What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?

FAQ ID - 3838

浏览

What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?

FAQ ID - 3839

浏览

What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-?

FAQ ID - 3840

浏览

What PCR enzymes can be used following the first-strand synthesis?

FAQ ID - 3841

浏览

What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)?

FAQ ID - 3842

浏览

What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?

2857

浏览

Does the PyroMark LINE assay target all LINE sequences?

2860

浏览

What is the amplicon length of the PyroMark CpG LINE assay?

2856

浏览

How do I perform the PyroMark Q24 Advanced upgrade?

FAQ ID -3163

浏览

Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?

FAQ ID -3160

浏览

What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents?

FAQ ID -3165

浏览

How accurate and reliable is PyroMark Q24 in mutation analysis?

FAQ ID -2094

浏览

We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions?

FAQ ID - 3621

浏览

What is the use of the PyroMark Q24 Validation Oligo?

FAQ ID -2855

浏览

How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?

FAQ ID -2095

浏览

How long does a single run on the PyroMark Q24 take?

FAQ ID -2096

浏览

3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram

FAQ ID - 3843

浏览

When do I have to change the pulse settings/methods in a pyrosequencing run setup?

FAQ ID -2942

浏览

What is a PyroMark instrument method or instrument code?

FAQ ID -2941

浏览

Can the PyroMark Q96 CpG LINE assay be used with an ID system?

2861

浏览

How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused?

FAQ ID -2863

浏览

How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage.

FAQ ID -2878

浏览

Can unused wells in a pyrosequencing plate be used in the next run?

FAQ ID -2872

浏览

What is the reason for signals ceasing in the middle of a pyrosequencing run?

FAQ ID -2875

浏览

Is the CpG software included in the PyroMark instruments to study methylation status?

FAQ ID -2842

浏览

What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?

FAQ ID -2881

浏览

Where can I find explanations to the warning given by the PyroMark software after run data analysis?

FAQ ID -2874

浏览

Which heating block is recommended for the pyrosequencing annealing step?

FAQ ID -2838

浏览

How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations?

FAQ ID -2848

浏览

What kind of shaker should be used for the pyrosequencing binding step?

FAQ ID -2837

浏览

What is the reason for a high substrate peak in the pyrosequencing pyrogram?

FAQ ID -2879

浏览

How many CpG sites are analyzed by the PyroMark CpG LINE assay?

2858

浏览

Is there a user manual available for the PyroMark Assay design software?

FAQ ID -2851

浏览

Which operating system is compatible with PyroMark IdentiFire Software?

FAQ ID - 3340

浏览

How do I set up a PyroMark CpG Assay?

FAQ ID -2814

浏览

What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?

FAQ ID -2007

浏览

Will the primer sequence for the PyroMark CpG Assay be provided?

FAQ ID -2823

浏览

Does QIAGEN offer a design of Custom PyroMark CpG Assays?

FAQ ID -2818

浏览

How are the PyroMark CpG Assays reconstituted?

FAQ ID -2817

浏览

How are the PyroMark CpG Assays shipped and stored?

FAQ ID -2816

浏览

What are the features of PyroMark CpG Assays, for example, in terms of design and validation?

FAQ ID -2821

浏览

What is included in a PyroMark Custom Assay?

FAQ ID -2815

浏览

In which format can PyroMark CpG Assays be ordered?

FAQ ID -2824

浏览

Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?

FAQ ID -2822

浏览

Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?

FAQ ID -2862

浏览

What is the recommended amplicon size for CpG assays?

FAQ ID -2825

浏览

What kind of reading length can I expect when using pyrosequencing technology for sequence analysis?

FAQ ID -2216

浏览

Will dUTP in a PCR reaction affect pyrosequencing?

FAQ ID -2843

浏览

Which purity grade is recommended for pyrosequencing primers?

FAQ ID -2832

浏览

What concentration should be used for the sequencing primer in pyrosequencing?

FAQ ID -2826

浏览

What is the concentration of PyroMark Control Oligo?

FAQ ID -2846

浏览

What is the sample throughput of pyrosequencing systems?

FAQ ID -2215

浏览

How do I reduce background peaks in the pyrosequencing pyrogram?

FAQ ID -2877

浏览

What is the sensitivity limitation for pyrosequencing?

FAQ ID -2840

浏览

What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?

FAQ ID -2852

浏览

Which end of the PCR primer for pyrosequencing should be biotinylated?

FAQ ID -2839

浏览

How many nucleotides of a homopolymer can be resolved in pyrosequencing?

FAQ ID -2871

浏览

Where can I order the Streptavidin Sepharose beads for pyrosequencing?

FAQ ID -2850

浏览

What are critical steps during CTC enrichment?

3830

浏览

How pure are the CTCs enriched from blood samples using the AdnaTest?

3831

浏览

Can frozen blood be used for the procedure?

3832

浏览

Can the EZ2 AdnaTest be used for other body fluids than blood?

3833

浏览

Can Heparin blood be used?

3834

浏览

Can the kit be used on the EZ2 Connect?

FAQ ID - 3845

浏览

Can I use a different elution buffer?

FAQ ID - 3847

浏览

What is the average amount of cfDNA present in 1 ml plasma?

FAQ ID - 3846

浏览

Can the kit be used on the EZ1 Advanced XL?

FAQ ID - 3844

浏览

Do you have a protocol for purification of cytoplasmic RNA from animal cells?

FAQ ID -1257

浏览

What kits does QIAGEN offer to extract RNA from whole blood?

FAQ ID -304

浏览

What is the composition of Buffer RLT?

FAQ ID -2793

浏览

What is the key technical challenge in isolating high quality RNA from cell or tissue samples?

FAQ ID -2656

浏览

Do you have a protocol for the isolation of RNA from leukocytes in milk?

FAQ ID -952

浏览

What is the maximum binding capacity of RNeasy spin columns?

FAQ ID -290

浏览

Can QIAquick Kits be used to clean up RNA samples?

FAQ ID -490

浏览

Are RNeasy spin columns sold separately?

FAQ ID -159

浏览

Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?

FAQ ID -619

浏览

Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?

FAQ ID -467

浏览

How can I minimize degradation of RNA from my pancreas sample?

FAQ ID -624

浏览

What is the composition of Buffer RW1?

FAQ ID -2796

浏览

Do you have a protocol for purification of total DNA from crude lysates?

FAQ ID -1255

浏览

Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?

FAQ ID -917

浏览

Do you have a protocol for purification of total DNA from yeast?

FAQ ID -1253

浏览

Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?

FAQ ID -924

浏览

Do you have a protocol for purification of total DNA from insects?

FAQ ID -1254

浏览

How can I precipitate genomic DNA using isopropanol?

FAQ ID -2953

浏览

What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?

FAQ ID - 3447

浏览

Do you have a protocol for the isolation of genomic DNA from sperm?

FAQ ID -909

浏览

What is the composition of buffer AE?

FAQ ID -730

浏览

How do I safely inactivate biohazardous flow-through material?

FAQ ID -12

浏览

What is the advantage of running an analytical gel with fractions of my plasmid preparation?

FAQ ID -769

浏览

What is the recipe for LB?

FAQ ID -212

浏览

How do I know if my plasmid is a high- or low copy number type?

FAQ ID -350

浏览

Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?

FAQ ID -366

浏览

I left Buffer P1 at room temperature after addition of RNase A, what shall I do?

FAQ ID -859

浏览

How do I perform a DNA precipitation to concentrate my sample?

FAQ ID -305

浏览

With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?

FAQ ID -864

浏览

What is the recipe for 2x YT?

FAQ ID -213

浏览

What are the additional plasmid bands I see on my gel?

FAQ ID -1059

浏览

Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?

FAQ ID -896

浏览

What is the composition of Buffer PB?

FAQ ID -2791

浏览

Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?

FAQ ID -3102

浏览

Why is my plasmid DNA yield low?

FAQ ID -768

浏览

Why do I get genomic DNA contamination in my plasmid prep?

FAQ ID -353

浏览

Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?

FAQ ID -898

浏览

I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?

FAQ ID -1045

浏览

What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?

FAQ ID -861

浏览

What is the recipe for SOC medium?

FAQ ID -798

浏览

How much RNA does a typical mammalian cell contain?

FAQ ID -2946

浏览

Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?

FAQ ID -1037

浏览

What are the effects of low A260/A230 ratios in RNA preparations on downstream applications?

FAQ ID -2248

浏览

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

FAQ ID -728

浏览

Do I need to purchase any consumable for processing the kit on EZ2 Connect?

FAQ ID - 3849

浏览

Are we able to customize protocol on the EZ2 Connect?

FAQ ID - 3850

浏览

Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?

FAQ ID - 3851

浏览

Can the kit be run on EZ1 instruments?

FAQ ID - 3852

浏览

How can I check the integrity of RNA purified using RNeasy Kits?

FAQ ID -1024

浏览

What are the limits of detection of the QIAxcel Connect?

FAQ ID - 3853

浏览

When should the N2 bottle used with the QIAxcel Connect System be changed?

FAQ ID - 3854

浏览

What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID - 3855

浏览

Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?

FAQ ID - 3856

浏览

What are the differences between QIAxcel Advanced and QIAxcel Connect?

FAQ ID - 3857

浏览

Why does an intensity calibration of the gel cartridge for the QIAxcel Connect need to be performed?

FAQ ID - 3858

浏览

What is the QIAxcel Connect System?

FAQ ID - 3859

浏览

Can I combine your CGT dPCR assays with custom designed assays?

FAQ ID - 3861

浏览

How many assays can be multiplexed?

FAQ ID - 3862

浏览

In which channels can you detect the CGT dPCR assays?

FAQ ID - 3863

浏览

How can I resuspend the lyophilized assay?

FAQ ID - 3864

浏览

What quenchers are used?

FAQ ID - 3865

浏览

Do I need to use restriction enzymes when quantifying AAV genomes?

FAQ ID - 3866

浏览

Can the CGT dPCR assays also be used for the quantification of non-AAV samples?

FAQ ID - 3868

浏览

Can I use the CGT dPCR assays for the titer determination of RNA viruses?

FAQ ID - 3869

浏览

How do I know if the assays are compatible with the sample DNA of interest?

FAQ ID - 3870

浏览

Are the assays also compatible with qPCR?

FAQ ID - 3871

浏览

Can they be used with lentiviral vectors?

FAQ ID - 3872

浏览

Is restriction enzyme digestion required?

FAQ ID - 3875

浏览

dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter?

FAQ ID - 3876

浏览

My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate?

FAQ ID - 3877

浏览

Is dPCR sensitive to inhibition?

FAQ ID - 3880

浏览

Is DNA extraction from the samples needed?

FAQ ID - 3873

浏览

How much sample can I load for detecting host cell contamination?

FAQ ID - 3879

浏览

Where can I find a copy of the .kpf file for a BioSprint protocol?

FAQ ID -3073

浏览

Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?

FAQ ID -3075

浏览

What is the minimum number of samples that can be extracted on a BioSprint 96?

FAQ ID -3076

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Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?

FAQ ID -3077

浏览

Can I use specific primers with the FastLane Kits?

FAQ ID -3078

浏览

Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?

FAQ ID -3079

浏览

Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?

FAQ ID -3080

浏览

Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?

FAQ ID - 3881

浏览

Do we have specificity and sensitivity data for this kit?

FAQ ID - 3882

浏览

Do we have specificity and sensitivity data for this kit?

FAQ ID - 3883

浏览

Is it possible to scale up sample volume and reaction volume?

FAQ ID - 3884

浏览

What color channels are used?

FAQ ID - 3885

浏览

What is the percentage of false negative results that QIAGEN obtained during validations?

FAQ ID - 3886

浏览

What is the minimum and maximum concentration of effective RNA for the test?

FAQ ID - 3887

浏览

Anything important when starting the cycler run?

FAQ ID - 3888

浏览

Is it possible to use ABI instruments without ROX as a passive reference?

FAQ ID - 3889

浏览

What is the shelf-life of the Neo Assay Kit?

FAQ ID - 3890

浏览

Can the SARS-CoV-2 Neo Assay Kit detect all variants?

FAQ ID - 3891

浏览

Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?

FAQ ID - 3892

浏览

Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?

FAQ ID - 3893

浏览

Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay?

FAQ ID - 3894

浏览

Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?

FAQ ID - 3895

浏览

Which positive control should I use?

FAQ ID - 3896

浏览

How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?

FAQ ID - 3897

浏览

How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?

FAQ ID - 3898

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How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?

FAQ ID - 3899

浏览

Can Phoenix Hot Start Taq utilize cDNA as template for PCR?

FAQ ID - 3900

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Is Phoenix Hot Start Taq capable of multiplex PCR?

FAQ ID - 3901

浏览

How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?

FAQ ID - 3902

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When should I use Phoenix Hot Start Taq GC reaction Buffer?

FAQ ID - 3903

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What is the amplification length limit of Phoenix Hot Start Taq?

FAQ ID - 3904

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What is the fidelity/error rate of Phoenix Hot Start Taq?

FAQ ID - 3905

浏览

Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?

FAQ ID - 3906

浏览

How can yield for long targets be increased when using Phoenix Hot Start Taq?

FAQ ID - 3907

浏览

How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?

FAQ ID - 3908

浏览

How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?

FAQ ID - 3909

浏览

When should I use 5X VeraSeq GC Buffer?

FAQ ID - 3910

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What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3911

浏览

Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage?

FAQ ID - 3913

浏览

What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3912

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How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3914

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Will VeraSeq 2.0 DNA Polymerase incorporate dUTP?

FAQ ID - 3915

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Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme?

FAQ ID - 3916

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How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?

FAQ ID - 3917

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What denaturation temperature should be used in cycling conditions?

FAQ ID - 3918

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What annealing temperature should be used in the cycling conditions?

FAQ ID - 3919

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What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature?

FAQ ID - 3920

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How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?

FAQ ID - 3922

浏览

How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?

FAQ ID - 3921

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When should I use 5X VeraSeq GC Buffer?

FAQ ID - 3923

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What is the amplification length limit of VeraSeq ULtra DNA Polymerase?

FAQ ID - 3924

浏览

What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase?

FAQ ID - 3925

浏览

How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?

FAQ ID - 3926

浏览

Will VeraSeq ULtra DNA Polymerase incorporate dUTP?

FAQ ID - 3927

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Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang?

FAQ ID - 3928

浏览

Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme?

FAQ ID - 3929

浏览

What denaturation temperature should be used in the cycling conditions?

FAQ ID - 3931

浏览

What annealing temperature should be used in the cycling conditions?

FAQ ID - 3932

浏览

What are the ResDNA Quant Kit components?

FAQ ID - 3878

浏览

How can I load new protocols onto the QIAcube?

FAQ ID -1421

浏览

Are run reports and/or log files available on the QIAcube?

FAQ ID -1412

浏览

What applications are offered on the QIAcube?

FAQ ID -1403

浏览

Can the QIAcube heater/shaker be used independently from protocol runs?

FAQ ID -1422

浏览

What EZ1 DNA Investigator protocol is recommended for very precious samples?

FAQ ID -1154

浏览

When should the Normalization protocol be used?

FAQ ID -1155

浏览

What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?

FAQ ID - 3364

浏览

Does the EpiTect Hi-C Data Analysis Portal cost money?

FAQ ID - 143077

浏览

What is the effect of using amounts of input material per sample that fall outside the recommended range?

FAQ ID - 143064

浏览

What is the required amount of input material per sample for the EpiTect Hi-C Kit?

FAQ ID - 143063

浏览

Does QIAGEN provide data analysis to accompany the EpiTect Hi-C Kit?

FAQ ID - 143075

浏览

How can I extract DNA from a polyacrylamide (PAGE) gel?

FAQ ID -120

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Why does my DNA sample float out of the slot when loading it onto an agarose gel?

FAQ ID -205

浏览

What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?

FAQ ID -148

浏览

I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?

FAQ ID -756

浏览

Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?

FAQ ID -576

浏览

Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

FAQ ID -2032

浏览

Can the QIAcube be connected to a laboratory information management system?

FAQ ID -1411

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What are the dimensions and weight of the QIAcube?

FAQ ID -1407

浏览

What dedicated QIAcube Kits are available?

FAQ ID -2337

浏览

How can I decontaminate the QIAcube system?

FAQ ID -1419

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Can the QIAcube rotor and/or buckets be removed for cleaning?

FAQ ID -1410

浏览

Can the QIAcube centrifuge be used independently from protocol runs?

FAQ ID -1423

浏览

Do I need to buy special kits for the QIAcube?

FAQ ID -1402

浏览

Do I need to discard partially used QIAcube tip racks?

FAQ ID -1418

浏览

Can I program my own protocols for the QIAcube?

FAQ ID -1405

浏览

Can the CGT dPCR assays also be used for the analysis of AAV genome integrity?

FAQ ID - 3867

浏览

Is the QIAprep&amp CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN?

3787

浏览

Do you have a protocol for the isolation of viral RNA from stool?

FAQ ID -918

浏览

Which extraction kits are recommended?

FAQ ID - 3874

浏览

How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C?

FAQ ID - 3934

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What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)?

FAQ ID - 3935

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What is the amount of standard for the different kit?

FAQ ID - 3936

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How did you calculate the conversion factor?

FAQ ID - 3937

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What does the standard kit contain?

FAQ ID - 3938

浏览

What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)?

FAQ ID - 3939

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How long are sample lysates stable in the fridge/ freezer?

FAQ ID - 143765

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Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

FAQ ID -2027

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Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?

FAQ ID -2031

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After bisulfite conversion, can the DNA be stored?

FAQ ID -2409

浏览

Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?

FAQ ID - 3940

浏览

When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?

FAQ ID - 3941

浏览

What size of RNA can be expected?

FAQ ID - 3942

浏览

Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?

FAQ ID - 3947

浏览

When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit

FAQ ID - 3948

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Are there other options for paraffin removal?

FAQ ID - 3949

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FAQ ID - 3950

浏览

Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)?

FAQ ID - 3951

浏览

An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away.

FAQ ID - 3952

浏览

Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue?

FAQ ID - 3953

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What size of RNA can be expected?

FAQ ID - 3954

浏览

Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID - 3955

浏览

My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?

FAQ ID - 3956

浏览

Why are my DV200 values of my RNA sample so low?

FAQ ID - 3957

浏览

The integrity of my DNA sample (e.g. QIAxcel Connect) is bad.

FAQ ID - 3958

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How do I proceed if the amount of starting material cannot be calculated in detail?

FAQ ID - 3943

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Are there other options for paraffin removal?

FAQ ID - 3946

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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID - 3944

浏览

My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?

FAQ ID - 3945

浏览

Is this available without glycerol?

FAQ ID - 3959

浏览

What types of priming are compatible with EnzScript M-MLV RT RNase H-?

FAQ ID - 3961

浏览

What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)?

FAQ ID - 3964

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What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?

FAQ ID - 3960

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What PCR enzymes can be used following first-strand synthesis?

FAQ ID - 3963

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Is the kit compatible with unpurified in-process samples?

FAQ ID - 3965

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Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration?

FAQ ID - 3971

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Do you recommend storage of highly diluted AAV samples?

FAQ ID - 3972

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Is it possible to store the lysates? How can the lysates be stored?

FAQ ID - 3973

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Can I use MspI or HpaII restriction enzymes from other suppliers?

FAQ ID - 3975

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How many AAV genome copies can I load into the QIAcuity?

FAQ ID - 3977

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Do you need to dilute the lysates before setting up the PCR mix?

FAQ ID - 3978

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How do I know if the CGT dPCR assays are compatible with the sample DNA of interest?

FAQ ID - 3980

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Are the CGT dPCR assays also compatible with qPCR?

FAQ ID - 3981

浏览

Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays?

FAQ ID - 3982

浏览

What is included in the SureSilencing shRNA Plasmids shipment?

FAQ ID -2785

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Is the QIAxcel Advanced discontinued?

FAQ ID - 3983

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What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit?

FAQ ID - 3984

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What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?

FAQ ID - 3985

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What water should I use to prepare the buffer concentrates?

FAQ ID - 3986

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What is the new Waste Tube made of?

FAQ ID - 3987

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Are the QIAwave kits based on a different chemistry than the legacy kits?

FAQ ID - 3990

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Is there a way to compare the environmental impacts of the kit?

FAQ ID - 3991

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Can the QIAwave kits be recycled?

FAQ ID - 3992

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Does the reuse of the Waste Tubes increase the risk of cross-contamination?

FAQ ID - 3988

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I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?

FAQ ID - 3989

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Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument?

FAQ ID - 3993

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Is the kit compatible with unpurified in-process samples?

FAQ ID - 3994

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My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction?

FAQ ID - 3995

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Can I use Internal Control as overall control?

FAQ ID - 3996

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How long can I store the reconstituted Internal Control and the reconstituted Positive Control?

FAQ ID - 3997

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Do you recommend storage of highly diluted mycoplasma samples?

FAQ ID - 3998

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What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit?

FAQ ID - 3999

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On which instrumentation will the RT² Profiler PCR Array work?

FAQ ID -2719

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Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?

FAQ ID -2569

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Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?

FAQ ID -2571

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Which human target is used for the quantification in the Investigator Quantiplex Kit?

FAQ ID -2577

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Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?

FAQ ID - 3728

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Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3?

FAQ ID -2570

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Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit?

FAQ ID - 3730

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Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit?

FAQ ID - 3743

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Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit?

FAQ ID - 3742

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Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System?

FAQ ID - 3732

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How long does a run take?

FAQ ID -2566

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Investigator Quantiplex: For which real-time cyclers has the kit been validated?

FAQ ID -2567

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What are the storage conditions?

FAQ ID - 3748

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Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?

FAQ ID -2568

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How can I streamline the quantification workflow?

FAQ ID - 3738

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Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q?

FAQ ID - 3729

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Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated?

FAQ ID - 3741

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Can I optimize a dPCR assay on the QIAcuity using gradient temperature?

FAQ ID - 3783

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?

FAQ ID -2126

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Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2130

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2131

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What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

FAQ ID -761

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Can I use REPLI-g for SNP Genotyping?

FAQ ID -700

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Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?

FAQ ID -2058

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What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?

FAQ ID - 3506

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Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?

FAQ ID -2595

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Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?

FAQ ID -717

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Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?

FAQ ID -1979

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?

FAQ ID -1452

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How is "Touchdown PCR" used to increase PCR specificity?

FAQ ID -75

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What should the starting template DNA quality and quantity be for PCR?

FAQ ID -74

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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?

FAQ ID -165

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Why do I get smeared PCR products?

FAQ ID -87

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Does QIAGEN sell Q-Solution separately?

FAQ ID -204

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Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?

FAQ ID -1745

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Is Q-Solution required for PCR with QIAGEN's PCR kits?

FAQ ID -380

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常见问题