How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?

Increase the polymerase concentration to 2 U/50µL for targets ≥ 1 kb.  Use of a PCR enhancer containing Betaine, DTT, BSA, and DMSO (3) may also be used to improve yield of complex targets (especially targets ≥ 3 kb). Increasing the extension time and/or the template concentration may also be helpful.