What types of priming are compatible with EnzScript M-MLV RT RNase H-?
EnzScript M-MLV RT RNase H- is compatible with random hexamer priming, poly oligo(dT)/anchored poly oligo(dT) priming, and gene-specific priming (GSP). Random hexamer priming is the most non-specific priming method and is commonly used for full coverage of fragmented RNA in library construction protocols. Poly oligo(dT) / anchored poly oligo(dT) is a more specific priming method where the primer hybridizes to the polyA tail of mRNA found in eukaryotes. GSP is the most specific priming method in which the primer hybridizes to a specific target or gene of interest.
As a general precaution against RNase degradation of the RNA template, inclusion of an RNase Inhibitor (ENZ Y9240) is suggested especially when RNA input amount is low (< 100 ng).
As a general precaution against RNase degradation of the RNA template, inclusion of an RNase Inhibitor (ENZ Y9240) is suggested especially when RNA input amount is low (< 100 ng).